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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Mutant Stock; Transgenic; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Generation ?+N1F11 (31-DEC-07) Donating Investigator Andrew McMahon, Harvard University Description
Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express the Cre recombinase under the control of the human keratin 14 promoter. Cre transcript is detected in the skin. When crossed to a reporter line containing Gt(ROSA)26Sortm1Sor, Beta-galactosidase activity is detected in the oral ectoderm at 11.75 dpc, and at 14.5 dpc activity is detected in the skin and throughout the dental epithelium. This strain represents an effective tool for generating tissue-specific targeted mutants that would be useful to study developmentally critical gene function in the ectoderm and its derivatives.Development
A transgenic construct containing sequence encoding cre recombinase under the control of a human keratin 14 promoter/enhancer with the human growth hormone gene and a polyadenylation signal, was introduced into B6CBAF1 donor eggs.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Wildtype mice from the colony can be used as controls. | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of KRT14
006403 129S.B6-Tg(KRT14-Esr1/HRAS)1Pkha/J 006661 129S.B6-Tg(KRT14-RAF1/ESR1)1Pkha/J 004853 B6.C3-Tg(KRT14-Birc5)19Gros/J 007622 B6;SJL-Tg(KRT14-Hr)551Cct/J 007678 B6;SJL-Tg(KRT14-rtTA)208Jek/J 002980 C57BL/6-Tg(Cd152Ig)1Jbs/J 006822 FVB-Tg(KRT14-MAP2K1/Esr1)12Pkha/J 005705 FVB-Tg(KRT14-Vegfa)3Dtm/J 008099 FVB-Tg(KRT14-rtTA)F42Efu/J 005107 STOCK Tg(KRT14-cre/Esr1)20Efu/J View Strains carrying other alleles of KRT14 (10 strains)
Additional Web Information
Cre-lox Systems
Genetic Quality Control Annual Report
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System (Cre Recombinase Expression)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(KRT14-cre)1Amc | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Andrew P McMahon | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | K14 cre; K14-cre; K14cre; | ||
| Mutation Made By | Joe Vaughan, Harvard University | ||
| Strain of Origin | (C57BL/6 x CBA)F1 | ||
| Site of Expression | skin, the oral ectoderm including the dental lamina at 11.75 d.p.c., and the dental epithelium by 14.5 d.p.c. | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | KRT14, keratin 14, human | ||
| General Note |
This allele is representative of 2 founders (#199 and #200) exhibiting the highest level of transgene expression. Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. | ||
| Molecular Note | The transgene is composed of a cre recombinase gene under the control of a human keratin 14 promoter. The human KRT14 promoter directs transgene expression in hair and skin. The transgene also contains a human growth hormone sequence and polyadenylationsignal. When crossed to a transgenic reporter line, beta-galactosidase activity was detected in the oral ectoderm at 11.75 dpc, and at 14.5 dpc activity is detected in the skin and throughout the dental epithelium. [MGI Ref ID J:65294] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Quantitative PCR, QPCR, vers. 1
Generic Cre, STD PCR, vers. 1
Tg(KRT14-cre)1Amc, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Dassule HR; Lewis P; Bei M; Maas R; McMahon AP. 2000. Sonic hedgehog regulates growth and morphogenesis of the tooth Development 127(22):4775-85. [PubMed: 11044393] [MGI Ref ID J:65294]
Additional References
Tg(KRT14-cre)1Amc relatedAzarova AM; Lyu YL; Lin CP; Tsai YC; Lau JY; Wang JC; Liu LF. 2007. From the Cover: Roles of DNA topoisomerase II isozymes in chemotherapy and secondary malignancies. Proc Natl Acad Sci U S A 104(26):11014-9. [PubMed: 17578914] [MGI Ref ID J:125761]
Cang Y; Zhang J; Nicholas SA; Kim AL; Zhou P; Goff SP. 2007. DDB1 is essential for genomic stability in developing epidermis. Proc Natl Acad Sci U S A 104(8):2733-7. [PubMed: 17301228] [MGI Ref ID J:125911]
Chen J; Den Z; Koch PJ. 2008. Loss of desmocollin 3 in mice leads to epidermal blistering. J Cell Sci 121(Pt 17):2844-9. [PubMed: 18682494] [MGI Ref ID J:140129]
Gritli-Linde A; Bei M; Maas R; Zhang XM; Linde A; McMahon AP. 2002. Shh signaling within the dental epithelium is necessary for cell proliferation, growth and polarization. Development 129(23):5323-37. [PubMed: 12403705] [MGI Ref ID J:80081]
Gritli-Linde A; Hallberg K; Harfe BD; Reyahi A; Kannius-Janson M; Nilsson J; Cobourne MT; Sharpe PT; McMahon AP; Linde A. 2007. Abnormal hair development and apparent follicular transformation to mammary gland in the absence of hedgehog signaling. Dev Cell 12(1):99-112. [PubMed: 17199044] [MGI Ref ID J:117334]
Klein OD; Lyons DB; Balooch G; Marshall GW; Basson MA; Peterka M; Boran T; Peterkova R; Martin GR. 2008. An FGF signaling loop sustains the generation of differentiated progeny from stem cells in mouse incisors. Development 135(2):377-85. [PubMed: 18077585] [MGI Ref ID J:130572]
Rice R; Spencer-Dene B; Connor EC; Gritli-Linde A; McMahon AP; Dickson C; Thesleff I; Rice DP. 2004. Disruption of Fgf10/Fgfr2b-coordinated epithelial-mesenchymal interactions causes cleft palate. J Clin Invest 113(12):1692-700. [PubMed: 15199404] [MGI Ref ID J:90909]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry The resulting chimeric animals were initially crossed to C57BL/6 mice, and then crossed to outbred Swiss Webster mice. Mice from two founder lines (199 and 200) were crossed with Shhn (null allele) mice for an unknown number of times. The Shhnallele was bred out of the colony at The Jackson Laboratory. Mating System Homozygote x Homozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $155.60 Female or Male Homozygous for Tg(KRT14-cre)1Amc *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $311.20 Homozygous for Tg(KRT14-cre)1Amc x Homozygous for Tg(KRT14-cre)1Amc
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $202.30 Female or Male Homozygous for Tg(KRT14-cre)1Amc *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $404.60 Homozygous for Tg(KRT14-cre)1Amc x Homozygous for Tg(KRT14-cre)1Amc
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Wildtype mice from the colony can be used as controls. | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice & Services Conditions of Use
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