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| Transgenic hemizygous mice express the Cre recombinase under the control of the human keratin 14 promoter. When these mice are crossed to a reporter line containing Gt(ROSA)26Sortm1Sor, Beta-galactosidase activity is detected in the oral ectoderm at 11.75 dpc, and in skin and throughout the dental epithelium at 14.5 dpc. This strain represents an effective tool for generating tissue-specific targeted mutants that would be useful to study developmentally critical gene function in the ectoderm and its derivatives. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Homozygote (Female x Male) 01-MAR-06 Species laboratory mouse Generation ?+N1F22 (31-DEC-07)
Generation DefinitionsDonating Investigator Andrew P McMahon, University of Southern California Description
Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express the Cre recombinase under the control of the human keratin 14 promoter. Cre transcript is detected in the skin. When crossed to a reporter line containing Gt(ROSA)26Sortm1Sor, Beta-galactosidase activity is detected in the oral ectoderm at 11.75 dpc, and at 14.5 dpc activity is detected in the skin and throughout the dental epithelium. This strain represents an effective tool for generating tissue-specific targeted mutants that would be useful to study developmentally critical gene function in the ectoderm and its derivatives.View cre expression characterization.
Development
A transgenic construct containing sequence encoding cre recombinase under the control of a human keratin 14 promoter/enhancer with the human growth hormone gene and a polyadenylation signal, was introduced into B6CBAF1 donor eggs.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
Related Strains
Facebase: tools
006084 B6.129P2(Cg)-Foxg1tm1(cre)Skm/J 003771 B6.Cg-Tg(Nes-cre)1Kln/J 005584 B6.Cg-Tg(Prrx1-cre)1Cjt/J 009388 B6;129S1-Osr2tm2(cre)Jian/J 006774 FVB-Tg(Col2a1-cre/ERT)KA3Smac/J 012719 STOCK Tgfb3tm1(cre)Vk/J 005107 STOCK Tg(KRT14-cre/ERT)20Efu/J 003829 STOCK Tg(Wnt1-cre)11Rth Tg(Wnt1-GAL4)11Rth/J View Facebase: tools (8 strains)
006403 129S.B6-Tg(KRT14-Esr1/HRAS)1Pkha/J 006661 129S.B6-Tg(KRT14-RAF1/ESR1)1Pkha/J 004853 B6.C3-Tg(KRT14-Birc5)19Gros/J 009687 B6.Cg-Tg(KRT14-Kitl*)4XTG2Bjl/J 007622 B6;SJL-Tg(KRT14-Hr)551Cct/J 007678 B6;SJL-Tg(KRT14-rtTA)208Jek/J 002980 C57BL/6-Tg(Cd152Ig)1Jbs/J 006822 FVB-Tg(KRT14-MAP2K1/Esr1)12Pkha/J 005705 FVB-Tg(KRT14-Vegfa)3Dtm/J 008099 FVB-Tg(KRT14-rtTA)F42Efu/J 017005 FVB.Cg-Tg(KRT14-Fyn*)aJsey/J 005107 STOCK Tg(KRT14-cre/ERT)20Efu/J
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedDevelopmental Biology Research
Craniofacial and Palate Defects
Orofacial clefting-specific cre expression
Research Tools
Cre-lox System
Cre Recombinase Expression
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(KRT14-cre)1Amc | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Andrew P McMahon | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | K14 cre; K14-cre; K14cre; Krt14Cre; | ||
| Mutation Made By | Joe Vaughan, Harvard University | ||
| Strain of Origin | (C57BL/6 x CBA)F1 | ||
| Site of Expression | skin, the oral ectoderm including the dental lamina at 11.75 d.p.c., and the dental epithelium by 14.5 d.p.c. | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | KRT14, keratin 14, human | ||
| Driver Note | KRT14 | ||
| General Note |
This allele is representative of 2 founders (#199 and #200) exhibiting the highest level of transgene expression. Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. | ||
| Molecular Note | The transgene is composed of a cre recombinase gene under the control of a human keratin 14 promoter. The human KRT14 promoter directs transgene expression in hair and skin. The transgene also contains a human growth hormone sequence and polyadenylationsignal. When crossed to a transgenic reporter line, beta-galactosidase activity was detected in the oral ectoderm at 11.75 dpc, and at 14.5 dpc activity is detected in the skin and throughout the dental epithelium. [MGI Ref ID J:65294] | ||
| Gene Symbol and Name | Tg(KRT14-cre)1Amc, transgene insertion 1, Andrew P McMahon | ||
| Chromosome | UN | ||
| Gene Common Name(s) | K14 cre; K14-cre; K14cre; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Quantitative PCR, QPCR
Generic Cre, Standard PCR
Tg(KRT14-cre)1Amc, Melt Curve Analysis
Tg(KRT14-cre)1Amc, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Dassule HR; Lewis P; Bei M; Maas R; McMahon AP. 2000. Sonic hedgehog regulates growth and morphogenesis of the tooth Development 127(22):4775-85. [PubMed: 11044393] [MGI Ref ID J:65294]
Additional References
Tg(KRT14-cre)1Amc relatedAhn Y; Sanderson BW; Klein OD; Krumlauf R. 2010. Inhibition of Wnt signaling by Wise (Sostdc1) and negative feedback from Shh controls tooth number and patterning. Development 137(19):3221-31. [PubMed: 20724449] [MGI Ref ID J:168361]
Ahn Y; Sims C; Logue JM; Weatherbee SD; Krumlauf R. 2013. Lrp4 and Wise interplay controls the formation and patterning of mammary and other skin appendage placodes by modulating Wnt signaling. Development 140(3):583-93. [PubMed: 23293290] [MGI Ref ID J:194074]
Azarova AM; Lyu YL; Lin CP; Tsai YC; Lau JY; Wang JC; Liu LF. 2007. From the Cover: Roles of DNA topoisomerase II isozymes in chemotherapy and secondary malignancies. Proc Natl Acad Sci U S A 104(26):11014-9. [PubMed: 17578914] [MGI Ref ID J:125761]
Blanco S; Kurowski A; Nichols J; Watt FM; Benitah SA; Frye M. 2011. The RNA-methyltransferase Misu (NSun2) poises epidermal stem cells to differentiate. PLoS Genet 7(12):e1002403. [PubMed: 22144916] [MGI Ref ID J:179810]
Breart B; Ramos-Perez WD; Mendoza A; Salous AK; Gobert M; Huang Y; Adams RH; Lafaille JJ; Escalante-Alcalde D; Morris AJ; Schwab SR. 2011. Lipid phosphate phosphatase 3 enables efficient thymic egress. J Exp Med 208(6):1267-78. [PubMed: 21576386] [MGI Ref ID J:176831]
Cang Y; Zhang J; Nicholas SA; Kim AL; Zhou P; Goff SP. 2007. DDB1 is essential for genomic stability in developing epidermis. Proc Natl Acad Sci U S A 104(8):2733-7. [PubMed: 17301228] [MGI Ref ID J:125911]
Chakrabarti R; Hwang J; Andres Blanco M; Wei Y; Lukacisin M; Romano RA; Smalley K; Liu S; Yang Q; Ibrahim T; Mercatali L; Amadori D; Haffty BG; Sinha S; Kang Y. 2012. Elf5 inhibits the epithelial-mesenchymal transition in mammary gland development and breast cancer metastasis by transcriptionally repressing Snail2. Nat Cell Biol 14(11):1212-22. [PubMed: 23086238] [MGI Ref ID J:194019]
Chakrabarti R; Wei Y; Romano RA; DeCoste C; Kang Y; Sinha S. 2012. Elf5 regulates mammary gland stem/progenitor cell fate by influencing notch signaling. Stem Cells 30(7):1496-508. [PubMed: 22523003] [MGI Ref ID J:194574]
Chen D; Jarrell A; Guo C; Lang R; Atit R. 2012. Dermal beta-catenin activity in response to epidermal Wnt ligands is required for fibroblast proliferation and hair follicle initiation. Development 139(8):1522-33. [PubMed: 22434869] [MGI Ref ID J:183485]
Chen J; Den Z; Koch PJ. 2008. Loss of desmocollin 3 in mice leads to epidermal blistering. J Cell Sci 121(Pt 17):2844-9. [PubMed: 18682494] [MGI Ref ID J:140129]
Cheng X; Jin J; Hu L; Shen D; Dong XP; Samie MA; Knoff J; Eisinger B; Liu ML; Huang SM; Caterina MJ; Dempsey P; Michael LE; Dlugosz AA; Andrews NC; Clapham DE; Xu H. 2010. TRP channel regulates EGFR signaling in hair morphogenesis and skin barrier formation. Cell 141(2):331-43. [PubMed: 20403327] [MGI Ref ID J:164741]
Choi YS; Chakrabarti R; Escamilla-Hernandez R; Sinha S. 2009. Elf5 conditional knockout mice reveal its role as a master regulator in mammary alveolar development: failure of Stat5 activation and functional differentiation in the absence of Elf5. Dev Biol 329(2):227-41. [PubMed: 19269284] [MGI Ref ID J:148362]
Croyle MJ; Lehman JM; O'Connor AK; Wong SY; Malarkey EB; Iribarne D; Dowdle WE; Schoeb TR; Verney ZM; Athar M; Michaud EJ; Reiter JF; Yoder BK. 2011. Role of epidermal primary cilia in the homeostasis of skin and hair follicles. Development 138(9):1675-85. [PubMed: 21429982] [MGI Ref ID J:171221]
Cui CY; Childress V; Piao Y; Michel M; Johnson AA; Kunisada M; Ko MS; Kaestner KH; Marmorstein AD; Schlessinger D. 2012. Forkhead transcription factor FoxA1 regulates sweat secretion through Bestrophin 2 anion channel and Na-K-Cl cotransporter 1. Proc Natl Acad Sci U S A :. [PubMed: 22223659] [MGI Ref ID J:179924]
Driskell I; Oda H; Blanco S; Nascimento E; Humphreys P; Frye M. 2012. The histone methyltransferase Setd8 acts in concert with c-Myc and is required to maintain skin. EMBO J 31(3):616-29. [PubMed: 22117221] [MGI Ref ID J:181779]
Economou AD; Ohazama A; Porntaveetus T; Sharpe PT; Kondo S; Basson MA; Gritli-Linde A; Cobourne MT; Green JB. 2012. Periodic stripe formation by a Turing mechanism operating at growth zones in the mammalian palate. Nat Genet 44(3):348-51. [PubMed: 22344222] [MGI Ref ID J:181644]
Egawa G; Osawa M; Uemura A; Miyachi Y; Nishikawa S. 2009. Transient expression of ephrin b2 in perinatal skin is required for maintenance of keratinocyte homeostasis. J Invest Dermatol 129(10):2386-95. [PubMed: 19571816] [MGI Ref ID J:157054]
Evers BM; Farooqi MS; Shelton JM; Richardson JA; Goldstein JL; Brown MS; Liang G. 2010. Hair growth defects in Insig-deficient mice caused by cholesterol precursor accumulation and reversed by simvastatin. J Invest Dermatol 130(5):1237-48. [PubMed: 20090767] [MGI Ref ID J:159927]
Flowers MT; Paton CM; O'Byrne SM; Schiesser K; Dawson JA; Blaner WS; Kendziorski C; Ntambi JM. 2011. Metabolic changes in skin caused by scd1 deficiency: a focus on retinol metabolism. PLoS One 6(5):e19734. [PubMed: 21573029] [MGI Ref ID J:172432]
Foijer F; DiTommaso T; Donati G; Hautaviita K; Xie SZ; Heath E; Smyth I; Watt FM; Sorger PK; Bradley A. 2013. Spindle checkpoint deficiency is tolerated by murine epidermal cells but not hair follicle stem cells. Proc Natl Acad Sci U S A 110(8):2928-33. [PubMed: 23382243] [MGI Ref ID J:194539]
Franzke CW; Cobzaru C; Triantafyllopoulou A; Loffek S; Horiuchi K; Threadgill DW; Kurz T; van Rooijen N; Bruckner-Tuderman L; Blobel CP. 2012. Epidermal ADAM17 maintains the skin barrier by regulating EGFR ligand-dependent terminal keratinocyte differentiation. J Exp Med 209(6):1105-19. [PubMed: 22565824] [MGI Ref ID J:189051]
Funato N; Nakamura M; Richardson JA; Srivastava D; Yanagisawa H. 2012. Tbx1 regulates oral epithelial adhesion and palatal development. Hum Mol Genet 21(11):2524-37. [PubMed: 22371266] [MGI Ref ID J:183770]
Genetic Resource Sciences at The Jackson Laboratory. 2012. Expression/Specificity Patterns of Cre Alleles, 2012 MGI Direct Data Submission :. [MGI Ref ID J:184579]
Genetic Resource Sciences at The Jackson Laboratory. 2008. Expression/Specificity patterns of Cre transgenes MGI Direct Data Submission :. [MGI Ref ID J:137887]
Gonzalez-Gonzalez E; Ra H; Hickerson RP; Wang Q; Piyawattanametha W; Mandella MJ; Kino GS; Leake D; Avilion AA; Solgaard O; Doyle TC; Contag CH; Kaspar RL. 2009. siRNA silencing of keratinocyte-specific GFP expression in a transgenic mouse skin model. Gene Ther 16(8):963-72. [PubMed: 19474811] [MGI Ref ID J:173006]
Gritli-Linde A; Bei M; Maas R; Zhang XM; Linde A; McMahon AP. 2002. Shh signaling within the dental epithelium is necessary for cell proliferation, growth and polarization. Development 129(23):5323-37. [PubMed: 12403705] [MGI Ref ID J:80081]
Gritli-Linde A; Hallberg K; Harfe BD; Reyahi A; Kannius-Janson M; Nilsson J; Cobourne MT; Sharpe PT; McMahon AP; Linde A. 2007. Abnormal hair development and apparent follicular transformation to mammary gland in the absence of hedgehog signaling. Dev Cell 12(1):99-112. [PubMed: 17199044] [MGI Ref ID J:117334]
Guo J; Feng Y; Barnes P; Huang FF; Idell S; Su DM; Shams H. 2012. Deletion of FoxN1 in the thymic medullary epithelium reduces peripheral T cell responses to infection and mimics changes of aging. PLoS One 7(4):e34681. [PubMed: 22514652] [MGI Ref ID J:187080]
Guo J; Rahman M; Cheng L; Zhang S; Tvinnereim A; Su DM. 2011. Morphogenesis and maintenance of the 3D thymic medulla and prevention of nude skin phenotype require FoxN1 in pre- and post-natal K14 epithelium. J Mol Med (Berl) 89(3):263-77. [PubMed: 21109991] [MGI Ref ID J:187504]
Huh SH; Narhi K; Lindfors PH; Haara O; Yang L; Ornitz DM; Mikkola ML. 2013. Fgf20 governs formation of primary and secondary dermal condensations in developing hair follicles. Genes Dev 27(4):450-8. [PubMed: 23431057] [MGI Ref ID J:193958]
Ishikawa TO; Herschman HR. 2010. Conditional Bicistronic Cre Reporter Line Expressing Both Firefly Luciferase and beta-galactosidase. Mol Imaging Biol :. [PubMed: 20495880] [MGI Ref ID J:165837]
Klein OD; Lyons DB; Balooch G; Marshall GW; Basson MA; Peterka M; Boran T; Peterkova R; Martin GR. 2008. An FGF signaling loop sustains the generation of differentiated progeny from stem cells in mouse incisors. Development 135(2):377-85. [PubMed: 18077585] [MGI Ref ID J:130572]
Kurosaka H; Islam MN; Kuremoto K; Hayano S; Nakamura M; Kawanabe N; Yanagita T; Rice DP; Harada H; Taniuchi I; Yamashiro T. 2011. Core binding factor beta functions in the maintenance of stem cells and orchestrates continuous proliferation and differentiation in mouse incisors. Stem Cells 29(11):1792-803. [PubMed: 21898689] [MGI Ref ID J:190203]
Kyrylkova K; Kyryachenko S; Biehs B; Klein O; Kioussi C; Leid M. 2012. BCL11B regulates epithelial proliferation and asymmetric development of the mouse mandibular incisor. PLoS One 7(5):e37670. [PubMed: 22629441] [MGI Ref ID J:187316]
Landrette SF; Cornett JC; Ni TK; Bosenberg MW; Xu T. 2011. piggyBac transposon somatic mutagenesis with an activated reporter and tracker (PB-SMART) for genetic screens in mice. PLoS One 6(10):e26650. [PubMed: 22039523] [MGI Ref ID J:179725]
Larrieu-Lahargue F; Welm AL; Thomas KR; Li DY. 2010. Netrin-4 induces lymphangiogenesis in vivo. Blood 115(26):5418-26. [PubMed: 20407033] [MGI Ref ID J:162807]
Lee DW; Zhao X; Yim YI; Eisenberg E; Greene LE. 2008. Essential role of cyclin-G-associated kinase (Auxilin-2) in developing and mature mice. Mol Biol Cell 19(7):2766-76. [PubMed: 18434600] [MGI Ref ID J:180119]
Lee R; Chang SY; Trinh H; Tu Y; White AC; Davies BS; Bergo MO; Fong LG; Lowry WE; Young SG. 2010. Genetic studies on the functional relevance of the protein prenyltransferases in skin keratinocytes. Hum Mol Genet 19(8):1603-17. [PubMed: 20106865] [MGI Ref ID J:158528]
Lee TC; Threadgill DW. 2009. Generation and validation of mice carrying a conditional allele of the epidermal growth factor receptor. Genesis 47(2):85-92. [PubMed: 19115345] [MGI Ref ID J:145161]
Leonoudakis D; Singh M; Mohajer R; Mohajer P; Fata JE; Campbell KP; Muschler JL. 2010. Dystroglycan controls signaling of multiple hormones through modulation of STAT5 activity. J Cell Sci 123(Pt 21):3683-92. [PubMed: 20940259] [MGI Ref ID J:182923]
Lu H; Lu Q; Zheng Y; Li Q. 2012. Notch signaling promotes the corneal epithelium wound healing. Mol Vis 18:403-11. [PubMed: 22355251] [MGI Ref ID J:191494]
Ma L; Lopez GF; Krimm RF. 2009. Epithelial-derived brain-derived neurotrophic factor is required for gustatory neuron targeting during a critical developmental period. J Neurosci 29(11):3354-64. [PubMed: 19295142] [MGI Ref ID J:147048]
Maklad A; Nicolai JR; Bichsel KJ; Evenson JE; Lee TC; Threadgill DW; Hansen LA. 2009. The EGFR is required for proper innervation to the skin. J Invest Dermatol 129(3):690-8. [PubMed: 18830272] [MGI Ref ID J:145069]
Maricich SM; Morrison KM; Mathes EL; Brewer BM. 2012. Rodents rely on Merkel cells for texture discrimination tasks. J Neurosci 32(10):3296-300. [PubMed: 22399751] [MGI Ref ID J:182735]
Ming M; Feng L; Shea CR; Soltani K; Zhao B; Han W; Smart RC; Trempus CS; He YY. 2011. PTEN Positively Regulates UVB-Induced DNA Damage Repair. Cancer Res 71(15):5287-95. [PubMed: 21771908] [MGI Ref ID J:174190]
Ming M; Shea CR; Feng L; Soltani K; He YY. 2011. UVA induces lesions resembling seborrheic keratoses in mice with keratinocyte-specific PTEN downregulation. J Invest Dermatol 131(7):1583-6. [PubMed: 21390050] [MGI Ref ID J:182077]
Mitchell K; Szekeres C; Milano V; Svenson KB; Nilsen-Hamilton M; Kreidberg JA; DiPersio CM. 2009. Alpha3beta1 integrin in epidermis promotes wound angiogenesis and keratinocyte-to-endothelial-cell crosstalk through the induction of MRP3. J Cell Sci 122(Pt 11):1778-87. [PubMed: 19435806] [MGI Ref ID J:150583]
Molyneux G; Geyer FC; Magnay FA; McCarthy A; Kendrick H; Natrajan R; Mackay A; Grigoriadis A; Tutt A; Ashworth A; Reis-Filho JS; Smalley MJ. 2010. BRCA1 basal-like breast cancers originate from luminal epithelial progenitors and not from basal stem cells. Cell Stem Cell 7(3):403-17. [PubMed: 20804975] [MGI Ref ID J:164435]
Morrison KM; Miesegaes GR; Lumpkin EA; Maricich SM. 2009. Mammalian Merkel cells are descended from the epidermal lineage. Dev Biol 336(1):76-83. [PubMed: 19782676] [MGI Ref ID J:154915]
Murphy MJ; Polok BK; Schorderet DF; Cleary ML. 2010. Essential role for Pbx1 in corneal morphogenesis. Invest Ophthalmol Vis Sci 51(2):795-803. [PubMed: 19797217] [MGI Ref ID J:160421]
Murthy A; Shao YW; Narala SR; Molyneux SD; Zuniga-Pflucker JC; Khokha R. 2012. Notch activation by the metalloproteinase ADAM17 regulates myeloproliferation and atopic barrier immunity by suppressing epithelial cytokine synthesis. Immunity 36(1):105-19. [PubMed: 22284418] [MGI Ref ID J:181258]
Nakrieko KA; Welch I; Dupuis H; Bryce D; Pajak A; St Arnaud R; Dedhar S; D'Souza SJ; Dagnino L. 2008. Impaired hair follicle morphogenesis and polarized keratinocyte movement upon conditional inactivation of integrin-linked kinase in the epidermis. Mol Biol Cell 19(4):1462-73. [PubMed: 18234842] [MGI Ref ID J:172934]
Oda Y; Hu L; Bul V; Elalieh H; Reddy JK; Bikle DD. 2012. Coactivator MED1 ablation in keratinocytes results in hair-cycling defects and epidermal alterations. J Invest Dermatol 132(4):1075-83. [PubMed: 22189783] [MGI Ref ID J:185244]
Ohazama A; Johnson EB; Ota MS; Choi HJ; Porntaveetus T; Oommen S; Itoh N; Eto K; Gritli-Linde A; Herz J; Sharpe PT. 2008. Lrp4 modulates extracellular integration of cell signaling pathways in development. PLoS ONE 3(12):e4092. [PubMed: 19116665] [MGI Ref ID J:144348]
Rice R; Spencer-Dene B; Connor EC; Gritli-Linde A; McMahon AP; Dickson C; Thesleff I; Rice DP. 2004. Disruption of Fgf10/Fgfr2b-coordinated epithelial-mesenchymal interactions causes cleft palate. J Clin Invest 113(12):1692-700. [PubMed: 15199404] [MGI Ref ID J:90909]
Sahu RP; Dasilva SC; Rashid B; Martel KC; Jernigan D; Mehta SR; Mohamed DR; Rezania S; Bradish JR; Armstrong AB; Warren S; Konger RL. 2012. Mice lacking epidermal PPARgamma exhibit a marked augmentation in photocarcinogenesis associated with increased UVB-induced apoptosis, inflammation and barrier dysfunction. Int J Cancer 131(7):E1055-66. [PubMed: 22467332] [MGI Ref ID J:186081]
Sampath H; Flowers MT; Liu X; Paton CM; Sullivan R; Chu K; Zhao M; Ntambi JM. 2009. Skin-specific deletion of stearoyl-CoA desaturase-1 alters skin lipid composition and protects mice from high fat diet-induced obesity. J Biol Chem 284(30):19961-73. [PubMed: 19429677] [MGI Ref ID J:152631]
Schlegelmilch K; Mohseni M; Kirak O; Pruszak J; Rodriguez JR; Zhou D; Kreger BT; Vasioukhin V; Avruch J; Brummelkamp TR; Camargo FD. 2011. Yap1 acts downstream of alpha-catenin to control epidermal proliferation. Cell 144(5):782-95. [PubMed: 21376238] [MGI Ref ID J:171057]
Shih MY; Kane MA; Zhou P; Yen CL; Streeper RS; Napoli JL; Farese RV Jr. 2009. Retinol Esterification by DGAT1 Is Essential for Retinoid Homeostasis in Murine Skin. J Biol Chem 284(7):4292-9. [PubMed: 19028692] [MGI Ref ID J:147611]
Singh P; Chen C; Pal-Ghosh S; Stepp MA; Sheppard D; Van De Water L. 2009. Loss of integrin alpha9beta1 results in defects in proliferation, causing poor re-epithelialization during cutaneous wound healing. J Invest Dermatol 129(1):217-28. [PubMed: 18633440] [MGI Ref ID J:144854]
Solovei I; Wang AS; Thanisch K; Schmidt CS; Krebs S; Zwerger M; Cohen TV; Devys D; Foisner R; Peichl L; Herrmann H; Blum H; Engelkamp D; Stewart CL; Leonhardt H; Joffe B. 2013. LBR and Lamin A/C Sequentially Tether Peripheral Heterochromatin and Inversely Regulate Differentiation. Cell 152(3):584-98. [PubMed: 23374351] [MGI Ref ID J:193454]
Verdoni AM; Ikeda S; Ikeda A. 2010. Serum response factor is essential for the proper development of skin epithelium. Mamm Genome 21(1-2):64-76. [PubMed: 20047077] [MGI Ref ID J:156871]
Wang XP; O'Connell DJ; Lund JJ; Saadi I; Kuraguchi M; Turbe-Doan A; Cavallesco R; Kim H; Park PJ; Harada H; Kucherlapati R; Maas RL. 2009. Apc inhibition of Wnt signaling regulates supernumerary tooth formation during embryogenesis and throughout adulthood. Development 136(11):1939-49. [PubMed: 19429790] [MGI Ref ID J:149542]
Wang Z; Zhang LJ; Guha G; Li S; Kyrylkova K; Kioussi C; Leid M; Ganguli-Indra G; Indra AK. 2012. Selective ablation of Ctip2/Bcl11b in epidermal keratinocytes triggers atopic dermatitis-like skin inflammatory responses in adult mice. PLoS One 7(12):e51262. [PubMed: 23284675] [MGI Ref ID J:195636]
Weber S; Niessen MT; Prox J; Lullmann-Rauch R; Schmitz A; Schwanbeck R; Blobel CP; Jorissen E; de Strooper B; Niessen CM; Saftig P. 2011. The disintegrin/metalloproteinase Adam10 is essential for epidermal integrity and Notch-mediated signaling. Development 138(3):495-505. [PubMed: 21205794] [MGI Ref ID J:170542]
Woo SH; Baba Y; Franco AM; Lumpkin EA; Owens DM. 2012. Excitatory glutamate is essential for development and maintenance of the piloneural mechanoreceptor. Development 139(4):740-8. [PubMed: 22241839] [MGI Ref ID J:181187]
Woo SH; Stumpfova M; Jensen UB; Lumpkin EA; Owens DM. 2010. Identification of epidermal progenitors for the Merkel cell lineage. Development 137(23):3965-71. [PubMed: 21041368] [MGI Ref ID J:166901]
Yanagida J; Hammiller B; Al-Matouq J; Behrens M; Trempus CS; Repertinger SK; Hansen LA. 2012. Accelerated elimination of ultraviolet-induced DNA damage through apoptosis in CDC25A-deficient skin. Carcinogenesis 33(9):1754-61. [PubMed: 22764135] [MGI Ref ID J:191020]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry The resulting chimeric animals were initially crossed to C57BL/6 mice, and then crossed to outbred Swiss Webster mice. Mice from two founder lines (199 and 200) were crossed with Shhn (null allele) mice for an unknown number of times. The Shhnallele was bred out of the colony at The Jackson Laboratory. Mating System Homozygote x Homozygote (Female x Male) 01-MAR-06 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Homozygous for Tg(KRT14-cre)1Amc
Price per Pair (US dollars $) Pair Genotype $464.00 Homozygous for Tg(KRT14-cre)1Amc x Homozygous for Tg(KRT14-cre)1Amc Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
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Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Homozygous for Tg(KRT14-cre)1Amc
Price per Pair (US dollars $) Pair Genotype $603.20 Homozygous for Tg(KRT14-cre)1Amc x Homozygous for Tg(KRT14-cre)1Amc Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.