Strain Name:

B10.BR-H2k2 H2-T18a/SgSnJJrep

Stock Number:

004804

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Availability:

Level 3

In addition to the H2k2 allele, this strain also carries the TL-18a variant allele of the thymic leukemia or TL antigen. This strain was derived from cryopreserved embryos of Stock No. 000465 because they predated an observed phenotypic change in the 000465 strain. Specifically a change in the CD4/CD8 T cell ratios and an increase in splenic leukocyte cellularity and a conspicuous elevation in cells of the monocyte lineage, was observed in that strain around 2003.

Description

Strain Information

Former Names B10.BR-H2k H2-T18a/SgSnJJrep    (Changed: 31-AUG-12 )
Type Congenic; Major Histocompatibility Congenic; Mutant Strain;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Mating SystemSibling x Sibling         (Female x Male)   01-MAR-06
Breeding Considerations This strain is a good breeder.
Specieslaboratory mouse
Background Strain C57BL/10SnSg
Donor Strain C57BR/cdJ
H2 HaplotypeH2k2
GenerationF1pF3 (14-AUG-14)
Generation Definitions

Appearance
black, may exhibit white belly spots
Related Genotype: a/a

Description
In approximately 2003 some phenotypic parameters of the B10.BR-H2k2 H2-T18a/SgSnJ mice were found to have changed relative to the strain's previous performance. In order to confirm that a change had occurred and in order to better characterize any differences that may have arisen, animals were recovered from cryopreserved embryos of the strain (Stock No. 004804) that had been frozen in 1991, and the two lines of B10.BR-H2k2 H2-T18a/SgSnJ were compared. This strain, which was the cryopreserved bankstock of 1991 that was recovered in approximately 2004, has the original phenotype and does not have the shift in the CD4/CD8 T cell ratios to an increased percentage of CD8 postitive T cells that is found in stock #465, the strain which has been continuously maintained live. Stock #465 also differs from this strain by having an increase in splenic leukocyte cellularity and a conspicuous elevation in cells of the monocyte lineage. Both colonies of B10.BR-H2k2 H2-T18a/SgSnJ (Stock No's. 000465 and 004804) may exhibit white belly spots; these spots are considered a strain characteristic.

Development
In 1965 Jack H. Stimpfling and Alberta Richardson reported making this congenic strain in which the H2 haplotype was backcrossed from C57BR/cdJ onto C57BL/10ScSn. This congenic was fixed homozygous at N9. In 1980 this congenic reached generation N9F34 and in 1991 embryos were generated for cryopreservation from males and females at generation N9F50 and N9F51. After cryoprservation the strain was maintained on the shelf as stock #00465 and in approximately 2003 a phenotypic deviation was noticed. The 1991 cryopreserved bankstock, which pre-dated the phenotypic deviation, was then cryorecovered and assigned stock #004804. In 2012 this strain reached generation N9F52p+F19.

Control Information

  Control
   000666 C57BL/10SnJ (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   H2-T18a allele
000469   B10.A-H2a H2-T18a/SgSnJ
000465   B10.BR-H2k2 H2-T18a/SgSnJ
001737   B6.Cg-Hrhr H2-T18a/J
View Strains carrying   H2-T18a     (3 strains)

View Strains carrying   H2k2     (3 strains)

View Strains carrying other alleles of H2-T18     (29 strains)

Strains carrying other alleles of H2
006500   129.NOD-(D17Mit175-H2)/J
001649   A.BY H2bc H2-T18f/SnJ-Dstncorn1/J
000140   A.BY-H2bc H2-T18f/SnJ
000472   A.CA-H2f H2-T18a/SnJ
000471   A.SW-H2s H2-T18b/SnJ
001066   A.TH-H2t2/SfDvEgMobJ
001067   A.TL-H2t1/SfDvEgMobJ
002089   AK.B6-H2b Fv1b/J
002090   AK.B6-H2b/J
001094   AK.L-H2b/1CyTyJ
001095   AK.L-H2oz2/CyJ
001096   AK.L-H2oz3/CyJ
000470   AK.M-H2m H2-T18a/nSnJ
003851   ALR.NOD-(D17Mit30-D17Mit123)/Lt
000469   B10.A-H2a H2-T18a/SgSnJ
000468   B10.A-H2h2/(2R)SgSnJ
001150   B10.A-H2h4/(4R)SgDvEgJ
001149   B10.A-H2i3/(3R)SgDvEgJ
000467   B10.A-H2i5 H2-T18a/(5R)SgSnJ
000466   B10.AKM-H2m H2-T18a/SnJ
001954   B10.AQR-H2y1/KljMcdJ
005308   B10.Cg-H2d Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ
005534   B10.Cg-H2d Tg(Ins2-HA)165Bri/ShrmJ
010514   B10.Cg-H2g Tg(Cd4-Klra1)6295Dl/J
006446   B10.Cg-H2h4 Sh3pxd2bnee/GrsrJ
006102   B10.Cg-H2k Tg(Il2/NFAT-luc)83Rinc/J
006100   B10.Cg-H2k Tg(NFkB/Fos-luc)26Rinc/J
005895   B10.Cg-Thy1a H2d Tg(TcraCl1,TcrbCl1)1Shrm/J
002024   B10.D1-H2q/SgJ
001163   B10.D2-H2bm23/EgJ
000462   B10.D2-H2d/n2SnJ
001164   B10.D2-H2dm1/EgJ
001151   B10.D2-H2g3/(103R)EgJ
001153   B10.D2-H2i7/(107R)EgJ
001152   B10.D2-H2ia/(106R)EgJ
000460   B10.D2-Hc0 H2d H2-T18c/o2SnJ
000461   B10.D2-Hc0 H2d H2-T18c/oSnJ
000463   B10.D2-Hc1 H2d H2-T18c/nSnJ
003147   B10.D2-Hc1 H2d H2-T18c/nSnJ-Tg(DO11.10)10Dlo/J
000464   B10.DA-H2qp1 H2-T18b/(80NS)SnJ
001823   B10.F-H2bp5/(14R)J
001818   B10.F-H2pb1/(13R)J
001012   B10.HTG-H2g/2CyJ
000999   B10.HTG-H2g/3CyJ
001894   B10.LG-H2ar1/J
000459   B10.M-H2f H2-T18a?/SnJ
002225   B10.M-H2f/nMob Fmn1ld-2J/J
001068   B10.M-H2f/nMobJ
000739   B10.M-H2fm2/MobJ
001154   B10.MBR-H2bq1/SxEgJ
010972   B10.NOD-(rs13459151-rs13483054)/1107MrkJ
001825   B10.P-H2kp1/(10R)SgJ
003199   B10.PL-H2u H2-T18a/(73NS)Sn-Tg(TCRA)B1Jg/J
003200   B10.PL-H2u H2-T18a/(73NS)Sn-Tg(TCRB)C14Jg/J
000458   B10.PL-H2u H2-T18a/(73NS)SnJ
000457   B10.RIII-H2r H2-T18b/(71NS)SnJ
001069   B10.RIII-H2r/(71NS)nMobJ
001760   B10.S-H2as1/(8R)/J
001953   B10.S-H2s/SgMcdJ
001817   B10.S-H2sm1/(12R)SgJ
001650   B10.S-H2t4/(9R)/J
000456   B10.SM H2v H2-T18b/(70NS)Sn-cw/J
001155   B10.T-H2y2/(6R)SgDvEgJ
000445   B10.WB-H2j H2-T18b/SnJ
000444   B10.Y-H2pa H2-T18c/SnJ
003483   B6 x B10.D1-H2q/SgJ-Nox3het-2J/J
003561   B6 x B10.PL-H2u/(73NS)Sn-Hxl/J
002995   B6 x C.B10-H2b/LiMcdJ-Fbn2fp-2J/J
003584   B6.129S2-H2dlAb1-Ea/J
001148   B6.AK-H2k/FlaEgJ
001895   B6.AK-H2k/J
001160   B6.C-H2bm10/KhEgJ
001161   B6.C-H2bm11/KhEgJ
000364   B6.C-H2bm2/ByJ
000369   B6.C-H2bm4/ByJ
001158   B6.C-H2bm7/KhEgJ
000360   B6.C-H2d Mdmg1BALB/cBy/aByJ
000359   B6.C-H2d/bByJ
001429   B6.C-H2g6/J
005715   B6.Cg H2g7-Tg(Ins2-CD80)3B7Flv/LwnJ
007958   B6.Cg-H2b3/FlaCmwJ
007959   B6.Cg-H2b4/FlaCmwJ
005717   B6.Cg-Sostdc1shk H2g7/GrsrJ
003068   B6.NOD-(Csf2-D11Mit42) (D17Mit21-D17Mit10)/J
004554   B6.NOD-(D17Mit21-D17Mit10) Tg(TCRaAI4)1Dvs/DvsJ
004555   B6.NOD-(D17Mit21-D17Mit10) Tg(TCRbAI4)1Dvs/DvsJ
003300   B6.NOD-(D17Mit21-D17Mit10)/LtJ
003069   B6.NOD-(D1Mit3-Bcl2) (D17Mit21-D17Mit10)/LtJ
003071   B6.NOD-(D1Mit5.1-D1Mit15) (D17Mit21-D17Mit10)/J
003067   B6.NOD-(D3Mit132-Tshb) (D17Mit21-D17Mit10)/J
003066   B6.NOD-(D6Mit54-D6Mit14) (D17Mit21-D17Mit10)/J
024949   B6.NOD-(D11Mit167) H2g7/DvsJ
025223   B6.NOD-(D11Mit167-D11Mit48) H2g7/DvsJ
000944   B6.SJL-H2b C3c/2CyJ
000966   B6.SJL-H2s C3c/1CyJ
000945   B6.SW/1CyJ
003374   B6;129S2-H2dlAb1-Ea/J
003240   B6;B10.A-H2a-Tg(H2KmPCC)2939Stoe/J
002844   BALB.5R-H2i5/LilJ
001165   BALB/c-H2dm2/KhEgJ
001041   BKS.B6-H2b/J
001892   BRVR.B10-H2b/J
001893   BRVR.D2-H2d/J
002845   C.B-H2b Tg(H2-Dd)D8Gja/LilJ
001952   C.B10-H2b/LilMcdJ
001768   C3.Cg-Irs1Sml H2b/GrsrJ
000443   C3.HTG-H2g H2-T18b?/SnJ
000441   C3.JK-H2j H2-T18b/SnJ
000440   C3.LG-H2ar1/CkcCyJ
000439   C3.NB-H2p H2-T18c?/SnJ
000438   C3.SW-H2b/SnJ
000473   C3H-H2o2 C4bb/SfSnJ
001156   C57BL/6J-H2bm3/EgJ
001157   C57BL/6Kh-H2bm5/KhEgJ
000437   D1.C-H2d H2-T18c/SnJ
000436   D1.DA-H2qp1/SnJ
000435   D1.LP-H2b H2-T18b?/SnJ
000434   LP.RIII-H2r H2-T18b/SnJ
001382   LT.MA-Glo1b H2d/J
001383   LT.MA-Glo1b H2k/J
002591   NOD.B10Sn-H2b/J
006935   NOD.Cg-H2b thnh/J
004447   NOD.Cg-H2h4/DilTacUmmJ
001626   NOD.NON-H2nb1/LtJ
002032   NOD.SW-H2q/J
001976   NOD/ShiLtJ
001627   NON.NOD-H2g7/LtJ
001308   STOCK H2473a/J
003154   WLC.C-H2d/MorJ
003153   WLC.Cg-H2d Mtv2a/MorJ
View Strains carrying other alleles of H2     (130 strains)

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol H2-T18a
Allele Name a variant
Allele Type Not Applicable
Common Name(s) Tlaa;
Gene Symbol and Name H2-T18, histocompatibility 2, T region locus 18
Chromosome 17
Gene Common Name(s) H-2T18; TL Ag; Tla; thymus leukemia antigen;
Molecular Note The allele H2-T18a determines presence of the antigenic specificities TL.1, 2, 3, 5, 6, 7 and occurs in strains A, C57BR, C58, SJL, and others.
 
Allele Symbol H2k2
Allele Name k2 variant
Allele Type Not Applicable
Common Name(s) H2k;
Gene Symbol and Name H2, histocompatibility-2, MHC
Chromosome 17
Gene Common Name(s) H-2; MHC-II;
Molecular Note The H2k2 haplotype differs from the prototype H2k in having the a, rather than the canonical b, alleles of the Class Ib genes T18 (formerly Tla) and Qa-1. The H2k2 haplotype is shared by the inbred strains C57BR/cdJ and C58/J and the H2 congenic strain B10.BR-H2k2 H2-T18a/SgSnJ (formerly B10.BR-H2k/SgSnJ). [MGI Ref ID J:41130]

Genotyping

Genotyping Information

The Jackson Laboratory has not developed a genotyping protocol for H2 haplotypes. The following reference may helpful: Saha BK. J Immunol Meth 1996 Jul 17;194(1):77-83. (PMID: 8690943). Alternatively, haplotype-specific antibodies can be used with FACS.

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

H2-T18a related

BOYSE EA; OLD LJ; LUELL S. 1964. GENETIC DETERMINATION OF THE TL (THYMUSLEUKAEMIA) ANTIGEN IN THE MOUSE. Nature 201:779. [PubMed: 14161199]  [MGI Ref ID J:13118]

Chen YT; Obata Y; Stockert E; Old LJ. 1985. Thymus-leukemia (TL) antigens of the mouse. Analysis of TL mRNA and TL cDNA TL+ and TL- strains. J Exp Med 162(4):1134-48. [PubMed: 3840195]  [MGI Ref ID J:8035]

Chorney MJ; Shen FW; Tung JS; Boyse EA. 1985. Additional products of the Tla locus of the mouse. Proc Natl Acad Sci U S A 82(20):7044-7. [PubMed: 3876563]  [MGI Ref ID J:8045]

Flaherty L; Rinchik E. 1980. A new allele and antigen at the Tla locus. Immunogenetics 11(2):205-8. [PubMed: 6157647]  [MGI Ref ID J:6368]

Gowdy KM; Nugent JL; Martinu T; Potts E; Snyder LD; Foster WM; Palmer SM. 2012. Protective role of T-bet and Th1 cytokines in pulmonary graft-versus-host disease and peribronchiolar fibrosis. Am J Respir Cell Mol Biol 46(2):249-56. [PubMed: 21960548]  [MGI Ref ID J:191909]

Shen FW; Chorney MJ; Boyse EA. 1982. Further polymorphism of the Tla locus defined by monoclonal TL antibodies. Immunogenetics 15(6):573-8. [PubMed: 7106865]  [MGI Ref ID J:6828]

Yamazaki K; Beauchamp GK; Bard J; Thomas L; Boyse EA. 1982. Chemosensory recognition of phenotypes determined by the Tla and H-2K regions of chromosome 17 of the mouse. Proc Natl Acad Sci U S A 79(24):7828-31. [PubMed: 6961454]  [MGI Ref ID J:6964]

H2k2 related

Fischer Lindahl K. 1997. On naming H2 haplotypes: functional significance of MHC class Ib alleles. Immunogenetics 46(1):53-62. [PubMed: 9148789]  [MGI Ref ID J:41130]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           MP13

Colony Maintenance

Mating SystemSibling x Sibling         (Female x Male)   01-MAR-06
Breeding Considerations This strain is a good breeder.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Weeks of AgePrice per mouse (US dollars $)GenderGenotypes Provided
3 weeks $54.50Female or MaleHomozygous for H2k, Homozygous for H2-T18a  
4 weeks $54.50Female or MaleHomozygous for H2k, Homozygous for H2-T18a  
5 weeks $54.50Female or MaleHomozygous for H2k, Homozygous for H2-T18a  
6 weeks $57.15Female or MaleHomozygous for H2k, Homozygous for H2-T18a  
7 weeks $59.80Female or MaleHomozygous for H2k, Homozygous for H2-T18a  
8 weeks $62.45Female or MaleHomozygous for H2k, Homozygous for H2-T18a  

Standard Supply

Level 3. Up to 50 mice. Larger quantities or custom orders arranged upon request.

Supply Notes

  • Shipped at a specific age in weeks. Mice at a precise age in days, littermates and retired breeders are also available.
Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Weeks of AgePrice per mouse (US dollars $)GenderGenotypes Provided
3 weeks $70.90Female or MaleHomozygous for H2k, Homozygous for H2-T18a  
4 weeks $70.90Female or MaleHomozygous for H2k, Homozygous for H2-T18a  
5 weeks $70.90Female or MaleHomozygous for H2k, Homozygous for H2-T18a  
6 weeks $74.30Female or MaleHomozygous for H2k, Homozygous for H2-T18a  
7 weeks $77.80Female or MaleHomozygous for H2k, Homozygous for H2-T18a  
8 weeks $81.20Female or MaleHomozygous for H2k, Homozygous for H2-T18a  

Standard Supply

Level 3. Up to 50 mice. Larger quantities or custom orders arranged upon request.

Supply Notes

  • Shipped at a specific age in weeks. Mice at a precise age in days, littermates and retired breeders are also available.
View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Level 3. Up to 50 mice. Larger quantities or custom orders arranged upon request.

Control Information

  Control
   000666 C57BL/10SnJ (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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