Strain Name: |
STOCK Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J |
|---|---|
Stock Number: |
004808 |
Availability: | Repository- Live |
Price and Supply Information | |
General Terms and Conditions |
| Former Name |
STOCK Mapttm1(GFP)Klt Tg(MAPT)8cPdav/J (Changed: 14-APR-06
) |
| Strain Common Names | htau; |
| Genes & Alleles | MAPT; Mapt; Mapttm1(EGFP)Klt; Pde6b; Pde6b+; Pde6brd1; Tg(MAPT)8cPdav; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Type JAX® GEMM® Strain - Transgenic Mating System See Colony Maintenance (Female x Male) Species laboratory mouse Donating Investigator Peter Davies, Albert Einstein College of Medicine Donating Investigator Karen Duff, Nathan Kline Institute Generation F12 (19-DEC-07) Important Note
This strain is segregating for the retinal degeneration allele Pde6brd1.Strain Description
Mice that are homozygous for the targeted allele and hemizygous for the transgene are viable and fertile. Although no endogenous mouse MAPT is detected, all six isoforms (including both 3R and 4R forms) of human MAPT are expressed. Hyperphosphorylated MAPT is detected in cell bodies and dendrites by 3 months of age. Paired helical filaments of aggregated insoluble MAPT can be isolated from brain tissue as early as 2 months of age. These mutant mice may be useful in studies examining the relationship between human MAPT and Alzheimer's disease pathogenesis.Strain Development
Double mutant mice were generated by crossing transgenic mice (mouse line 8C) with Mapt targeted mutant mice. The targeted mutant allele was created by inserting EGFP coding sequence into the first Mapt exon, disrupting expression of the Mapt gene and producing a cytoplasmic EGFP protein fused to the first 31 MAPT amino acids (see Stock Number 004779). The transgenic allele consists of a PAC insert of 200-250 kb that includes the coding sequence, intronic regions and regulatory elements of the human MAPT gene. The targeted allele was created in 129S4/SvJae-derived J1 embryonic stem cells which were subsequently injected into C57BL/6 blastocysts. The transgenic allele was generated in embryos derived from a cross between Swiss Webster females and B6D2F1 males.
Related Disease (OMIM) Terms |
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Mapttm1(EGFP)Klt | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Kerry Lee Tucker | ||
| Common Name(s) | Mapttm1(GFP)Klt; tau ko; | ||
| Mutation Made By | Kerry Tucker, Ruprecht Karls University of Heidelberg | ||
| Strain of Origin | 129S4/SvJae | ||
| ES Cell Line Name | J1 | ||
| ES Cell Line Strain | 129S4/SvJae | ||
| Site of Expression | GFP signal is detected beginning a embryonic day 9 in the trigeminal ganglion and throughout the developing central nervous system by embryonic day 10.75. GFP expression persists in adults and closely resembles the expression of neuron specific beta-tubulin III. | ||
| Gene Symbol and Name | Mapt, microtubule-associated protein tau | ||
| Chromosome | 11 | ||
| Gene Common Name(s) | AI413597; AW045860; DDPAC; FLJ31424; FTDP-17; MAPTL; MGC138549; MGC156663; MSTD; MTBT1; MTBT2; Mtapt; PPND; RNPTAU; TAU; Tau; expressed sequence AI413597; expressed sequence AW045860; pTau; | ||
| Molecular Note | Exon 1 was disrupted by the insertion of a cassette encoding EGFP and neo. Fusion protein including GFP was detected by Western blot analysis of homozygous mutant brain lysates. Fluorescent illumination indicated that expression of the reporter gene was similar to that of the endogenous gene. [MGI Ref ID J:66561] | ||
| Allele Symbol | Tg(MAPT)8cPdav | ||
| Allele Name | transgene insertion 8c, Peter Davies | ||
| Common Name(s) | 8c; | ||
| Mutation Made By | Peter Davies, Albert Einstein College of Medicine | ||
| Strain of Origin | Swiss Webster x (C57BL/6 x DBA)F1 | ||
| Site of Expression | Neurons: EGFP signal observed at 9.0 dpc (days post-coitum) in the trilgeminal ganglion. At 10.75 dpc. EGFP was seen throughout the developing nervous system. | ||
| Expressed Gene | MAPT, microtubule-associated protein tau, human | ||
| Promoter | MAPT, microtubule-associated protein tau, human | ||
| General Note | Mice that are homozygous for the Mapttm1(GFP)Klt allele and hemizygous for the transgene are viable and fertile. Although no endogenous mouse MAPT is detected, all six isoforms (including both 3R and 4R forms) of human MAPT are expressed. Hyperphosphorylated MAPT is detected in cell bodies and dendrites by 3 months of age. Paired helical filaments of aggregated insoluble MAPT can be isolated from brain tissue as early as 2months of age. | ||
| Molecular Note | The transgene contains the coding sequence, intronic regions, and regulatory elements of the human microtubule-associated protein tau (MAPT) gene. [MGI Ref ID J:85952] | ||
| Allele Symbol | Pde6b+ | ||
| Allele Name | wild type | ||
| Mutation Made By | Frank Kooy, University of Antwerp | ||
| Gene Symbol and Name | Pde6b, phosphodiesterase 6B, cGMP, rod receptor, beta polypeptide | ||
| Chromosome | 5 | ||
| Gene Common Name(s) | CSNB3; PDEB; Pdeb; RP40; nmf137; phosphodiesterase, cGMP, rod receptor, beta polypeptide; r; rd; rd-1; rd1; rd10; retinal degeneration; retinal degeneration 1; retinal degeneration 10; | ||
| Allele Symbol | Pde6brd1 | ||
| Allele Name | retinal degeneration 1 | ||
| Common Name(s) | rd; rd-1; rd1; rodless retina; | ||
| Gene Symbol and Name | Pde6b, phosphodiesterase 6B, cGMP, rod receptor, beta polypeptide | ||
| Chromosome | 5 | ||
| Gene Common Name(s) | CSNB3; PDEB; Pdeb; RP40; nmf137; phosphodiesterase, cGMP, rod receptor, beta polypeptide; r; rd; rd-1; rd1; rd10; retinal degeneration; retinal degeneration 1; retinal degeneration 10; | ||
| Molecular Note | Two mutations have been identified in rd1 mice. A murine leukimia virus (Xmv-28) insertion in reverse orientation in intron 1 is found in all mouse strains with the rd1 phenotype. Further, a nonsense mutation (C to A transversion) in codon 347 that results in a truncation eliminating more than half of the predicted encoded protein, including the catalytic domain has also been identified in all rd1 strains of mice. A specific degradation of mutant transcript during or after pre-mRNA splicing is suggested. [MGI Ref ID J:11513] [MGI Ref ID J:4366] [MGI Ref ID J:51361] | ||
Control Information
| Control | ||
|---|---|---|
| +/+ from the colony | ||
| Homozygote | ||
| Mice from the colony that are homozygous for the targeted mutation and wild type for the transgene should be considered for use as controls. The most appropriate control animal will depend on the nature of the experiment. | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Genotyping Protocols
Mapttm1(EGFP)Klt
Tg(MAPT)8cPdav
Colony Maintenance
| Breeding & Husbandry | The targeted allele is maintained in a homozygous state while the transgenic allele is maintained in a hemizygous state. Mice that are heterozygous for the Mapt targeted mutation and hemizygous for the MAPT transgene will be distributed. Breeder pairs will also be heterozygous for the targeted allele and hemizygous for the trangene by the same. Expected coat color is agouti. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (145 strains)
005491 B6.Cg-Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J 004779 STOCK Mapttm1(EGFP)Klt/J
002802 C3.BLiA Pde6b+-Krd/J 001979 C3A.BLiA-Pde6b+.O20-Prph2Rd2/J 001912 C3A.BLiA-Pde6b+/J 003648 C3Sn.BLiA-Pde6b+/Dn 004828 FVB.129P2-Pde6b+ Tyrc-ch/AntJ
005491 B6.Cg-Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J
008169 B6;C3-Tg(Prnp-MAPT*P301S)PS19Vle/J 003741 B6D2-Tg(Prnp-MAPT)43Vle/J
007251 B6.129-Mapttm1Hnd/J
004297 B6.CXB1-Pde6brd10/J 004766 C57BL/6J-Pde6brd1-2J/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Genetic Quality Control Annual Report
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
MAPT relatedNeurobiology Research
Alzheimer's Disease
Research Tools
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers: neurons)
Neurobiology Research (cell marker)
Mapttm1(EGFP)Klt relatedNeurobiology Research
Alzheimer's Disease
Parkinson's Disease
Pde6b+ relatedNeurobiology Research
Alzheimer's Disease (Tau (Mapt) mutants)
Pde6brd1 relatedMouse/Human Gene Homologs
retinitis pigmentosa, wildtype
Sensorineural Research
Retinal Degeneration (wild-type)
Tg(MAPT)8cPdav relatedMouse/Human Gene Homologs
retinitis pigmentosa, autosomal recessive
Sensorineural Research
Retinal Degeneration
Neurobiology Research
Alzheimer's Disease (Tau (Mapt) mutants)
References
Selected Reference(s)
Additional ReferencesAndorfer C; Kress Y; Espinoza M; de Silva R; Tucker KL; Barde YA; Duff K; Davies P. 2003. Hyperphosphorylation and aggregation of tau in mice expressing normal human tau isoforms. J Neurochem 86(3):582-90. [PubMed: 12859672] [MGI Ref ID J:84638]
Price and Supply Information
| Strain Name: | STOCK Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J |
| Stock Number: | 004808 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
USA, Canada and Mexico
| Price(s) in US dollars ($) | Genotype(s) Provided | ||||
|---|---|---|---|---|---|
| Individual Mouse Price | $236.40 | Homozygous for Mapttm1(EGFP)Klt, Hemizygous for Tg(MAPT)8cPdav | |||
| Pair | $472.80 | Homozygous for Mapttm1(EGFP)Klt, Hemizygous for Tg(MAPT)8cPdav x Homozygous for Mapttm1(EGFP)Klt, Hemizygous for Tg(MAPT)8cPdav | |||
| Pair | $465.90 | Homozygous for Mapttm1(EGFP)Klt, Hemizygous for Tg(MAPT)8cPdav x Homozygous for Mapttm1(EGFP)Klt, Noncarrier | |||
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-800-422-6423. Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form