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| These R26CreER mutant mice have a tamoxifen-inducible Cre-mediated recombination system driven by the endogenous mouse Gt(ROSA)26Sor promoter. When crossed with a strain containing a loxP site-flanked sequence of interest, this mutant is useful for generating tamoxifen-induced, Cre-mediated targeted deletions. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Homozygote (Female x Male) 01-MAR-06 Species laboratory mouse Generation F?+F11 (30-DEC-08) Donating Investigator Jeremy Nathans, Johns Hopkins University Description
These R26CreER mutant mice have a tamoxifen-inducible Cre-mediated recombination system driven by the endogenous mouse Gt(ROSA)26Sor promoter. The mutant allele consists of a fusion product involving Cre recombinase and an altered version of the mouse estrogen receptor ligand binding domain. The mutant ligand binding domain does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the CRE/ESR1 protein can only gain access to the nuclear compartment to mediate recombination after exposure to tamoxifen. Tamoxifen administration will also induce Cre recombination in the developing embryos of treated mothers. When crossed with a strain containing a loxP site-flanked sequence of interest, this mutant is useful for generating tamoxifen-induced, Cre-mediated targeted deletions. Homozygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.Development
A targeting vector containing a PGK-neo cassette and Cre recombinase/estrogen receptor ligand-binding domain fusion protein sequence was used to insert the fusion protein into a site located within intron 1 of the GT(ROSA)26Sor locus. The construct was introduced into 129X1/SvJ x 129S1/Sv-derived R1-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Correctly targeted ES cells were injected into C57BL/6J blastocysts to obtain chimeric animals.
Control Information
| Control | ||
|---|---|---|
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Gt(ROSA)26Sor
View Strains carrying other alleles of Gt(ROSA)26Sor (62 strains)
Additional Web Information
Genetic Quality Control Annual Report
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Gt(ROSA)26Sortm1(cre/Esr1)Nat/Gt(ROSA)26Sortm1(cre/Esr1)Nat
B6.129-Gt(ROSA)26Sortm1(cre/Esr1)Nat
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:84747)
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System
Cre Recombinase Expression: Inducible
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Gt(ROSA)26Sortm1(cre/Esr1)Nat relatedResearch Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Developmental Biology Research
Internal/Organ Defects
ovary; uterus
Internal/Organ Research
Other Organ Defects
Reproductive Biology Research
Developmental Defects Affecting Gonads
germ cell deficient
Fertility Defects
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Gt(ROSA)26Sortm1(cre/Esr1)Nat | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Jeremy Nathans | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | R26CreER; | ||
| Mutation Made By | Tudor Badea, Johns Hopkins University | ||
| Strain of Origin | 129 | ||
| Site of Expression | tamoxifen-inducible cre; when crossed with a strain containing a loxP site-flanked sequence of interest, this mutant is useful for generating tamoxifen-induced, Cre-mediated targeted deletions | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
| Driver Note | Gt(ROSA)26Sor | ||
| Molecular Note | A cDNA fragment encoding a fusion protein comprised of cre recombinase and the ligand binding domain of Esr1 was inserted at the endogenous locus via homologous recombination. The initiator methionine was placed in an optimal translational context, allowing for near ubiquitous expression via the endogenous promoter. Activation of cre recombinase activity is dependent on the administration of 4-HO-tamoxifen. [MGI Ref ID J:84747] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gt(Rosa)26Sortm1(cre/Esr1)Nat, Melt Curve Analysis
Gt(ROSA)26Sortm1(cre/Esr1)Nat, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Badea TC; Wang Y; Nathans J. 2003. A noninvasive genetic/pharmacologic strategy for visualizing cell morphology and clonal relationships in the mouse. J Neurosci 23(6):2314-22. [PubMed: 12657690] [MGI Ref ID J:84747]
Additional References
Gt(ROSA)26Sortm1(cre/Esr1)Nat relatedBadea TC; Cahill H; Ecker J; Hattar S; Nathans J. 2009. Distinct roles of transcription factors brn3a and brn3b in controlling the development, morphology, and function of retinal ganglion cells. Neuron 61(6):852-64. [PubMed: 19323995] [MGI Ref ID J:147350]
Chong MM; Rasmussen JP; Rundensky AY; Littman DR. 2008. The RNAseIII enzyme Drosha is critical in T cells for preventing lethal inflammatory disease. J Exp Med 205(9):2005-17. [PubMed: 18725527] [MGI Ref ID J:138683]
Cowley DO; Rivera-Perez JA; Schliekelman M; He YJ; Oliver TG; Lu L; O'Quinn R; Salmon ED; Magnuson T; Van Dyke T. 2009. Aurora-A kinase is essential for bipolar spindle formation and early development. Mol Cell Biol 29(4):1059-71. [PubMed: 19075002] [MGI Ref ID J:145744]
Das M; Jiang F; Sluss HK; Zhang C; Shokat KM; Flavell RA; Davis RJ. 2007. Suppression of p53-dependent senescence by the JNK signal transduction pathway. Proc Natl Acad Sci U S A 104(40):15759-64. [PubMed: 17893331] [MGI Ref ID J:131766]
Haldar M; Hedberg ML; Hockin MF; Capecchi MR. 2009. A CreER-based random induction strategy for modeling translocation-associated sarcomas in mice. Cancer Res 69(8):3657-64. [PubMed: 19351831] [MGI Ref ID J:147728]
Jones C; Roper VC; Foucher I; Qian D; Banizs B; Petit C; Yoder BK; Chen P. 2008. Ciliary proteins link basal body polarization to planar cell polarity regulation. Nat Genet 40(1):69-77. [PubMed: 18066062] [MGI Ref ID J:131308]
Liu C; Wang Y; Smallwood PM; Nathans J. 2008. An essential role for Frizzled5 in neuronal survival in the parafascicular nucleus of the thalamus. J Neurosci 28(22):5641-53. [PubMed: 18509025] [MGI Ref ID J:136390]
Lu TL; Chang JL; Liang CC; You LR; Chen CM. 2007. Tumor spectrum, tumor latency and tumor incidence of the pten-deficient mice. PLoS ONE 2(11):e1237. [PubMed: 18043744] [MGI Ref ID J:130367]
Mangale VS; Hirokawa KE; Satyaki PR; Gokulchandran N; Chikbire S; Subramanian L; Shetty AS; Martynoga B; Paul J; Mai MV; Li Y; Flanagan LA; Tole S; Monuki ES. 2008. Lhx2 selector activity specifies cortical identity and suppresses hippocampal organizer fate. Science 319(5861):304-9. [PubMed: 18202285] [MGI Ref ID J:130167]
Olson LE ; Soriano P. 2009. Increased PDGFRalpha activation disrupts connective tissue development and drives systemic fibrosis. Dev Cell 16(2):303-13. [PubMed: 19217431] [MGI Ref ID J:146617]
Qian D; Jones C; Rzadzinska A; Mark S; Zhang X; Steel KP; Dai X; Chen P. 2007. Wnt5a functions in planar cell polarity regulation in mice. Dev Biol 306(1):121-33. [PubMed: 17433286] [MGI Ref ID J:122585]
Takai H; Wang RC; Takai KK; Yang H; de Lange T. 2007. Tel2 regulates the stability of PI3K-related protein kinases. Cell 131(7):1248-59. [PubMed: 18160036] [MGI Ref ID J:141633]
Zheng L; Njauw CN; Martins-Green M. 2007. A hCXCR1 transgenic mouse model containing a conditional color-switching system for imaging of hCXCL8/IL-8 functions in vivo. J Leukoc Biol 82(5):1247-56. [PubMed: 17704296] [MGI Ref ID J:127327]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry This strain originated and is maintained on a B6;129 background as a homozygote. Mating System Homozygote x Homozygote (Female x Male) 01-MAR-06 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $96.30 Female or Male Homozygous for Gt(ROSA)26Sortm1(cre/Esr1)Nat
Pairs /Price (US dollars $) Pair Genotype $192.60 Homozygous for Gt(ROSA)26Sortm1(cre/Esr1)Nat x Homozygous for Gt(ROSA)26Sortm1(cre/Esr1)Nat
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $125.20 Female or Male Homozygous for Gt(ROSA)26Sortm1(cre/Esr1)Nat
Pairs /Price (US dollars $) Pair Genotype $250.40 Homozygous for Gt(ROSA)26Sortm1(cre/Esr1)Nat x Homozygous for Gt(ROSA)26Sortm1(cre/Esr1)Nat
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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