Strain Name: |
B6;129-Gt(ROSA)26Sortm1(cre/Esr1)Nat/J |
|---|---|
Stock Number: |
004847 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Gt(ROSA)26Sor; Gt(ROSA)26Sortm1(cre/Esr1)Nat; cre; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Jeremy Nathans, Johns Hopkins University Generation F?+9 (19-DEC-07) Strain Description
These mutant mice have a tamoxifen-inducible cre-mediated recombination system driven by the endogenous mouse Gt(ROSA)26Sor promoter. The mutant allele consists of a fusion product involving Cre recombinase and an altered version of the mouse estrogen receptor ligand binding domain. The mutant ligand binding domain does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the CRE/ESR1 protein can only gain access to the nuclear compartment to mediate recombination after exposure to tamoxifen. Tamoxifen administration will also induce CRE recombination in the developing embryos of treated mothers. When crossed with a strain containing a loxP site-flanked sequence of interest, this mutant is useful for generating tamoxifen-induced, CRE-mediated targeted deletions. Homozygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.Strain Development
A targeting vector containing a PGK-neo cassette and Cre recombinase/estrogen receptor ligand-binding domain fusion protein sequence was used to insert the fusion protein into a site located within intron 1 of the GT(ROSA)26Sor locus. The construct was introduced into 129X1/SvJ x 129S1/Sv-derived R1-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Correctly targeted ES cells were injected into C57BL/6J blastocysts to obtain chimeric animals.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Gt(ROSA)26Sortm1(cre/Esr1)Nat | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Jeremy Nathans | ||
| Common Name(s) | R26CreER; | ||
| Mutation Made By | Tudor Badea, Johns Hopkins University | ||
| Strain of Origin | 129 | ||
| Site of Expression | tamoxifen-inducible cre; when crossed with a strain containing a loxP site-flanked sequence of interest, this mutant is useful for generating tamoxifen-induced, Cre-mediated targeted deletions | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
| Molecular Note | A cDNA fragment encoding a fusion protein comprised of cre recombinase and the ligand binding domain of Esr1 was inserted at the endogenous locus via homologous recombination. The initiator methionine was placed in an optimal translational context, allowing for near ubiquitous expression via the endogenous promoter. Activation of cre recombinase activity is dependent on the administration of 4-HO-tamoxifen. [MGI Ref ID J:84747] | ||
Control Information
| Allele | Control | |
|---|---|---|
| Gt(ROSA)26Sortm1(cre/Esr1)Nat | 101045 B6129SF2/J | (approximate) |
| Considerations for Choosing Controls | ||
Genotyping Protocols
Gt(ROSA)26Sortm1(cre/Esr1)Nat
Colony Maintenance
| Breeding & Husbandry | This strain originated and is maintained on a B6;129 background as a homozygote. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Gt(ROSA)26Sor
View Strains carrying other alleles of Gt(ROSA)26Sor (36 strains)
Additional Web Information
Cre-lox or FLP-FRT Systems
Genetic Quality Control Annual Report
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System (Cre-Recombinase Expression: Inducible)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Gt(ROSA)26Sortm1(cre/Esr1)Nat relatedResearch Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Developmental Biology Research
Internal/Organ Defects (ovary; uterus)
Internal/Organ Research
Other Organ Defects
Reproductive Biology Research
Developmental Defects Affecting Gonads (germ cell deficient)
Fertility Defects
References
Selected Reference(s)
Additional ReferencesBadea TC; Wang Y; Nathans J. 2003. A noninvasive genetic/pharmacologic strategy for visualizing cell morphology and clonal relationships in the mouse. J Neurosci 23(6):2314-22. [PubMed: 12657690] [MGI Ref ID J:84747]
Price and Supply Information
| Strain Name: | B6;129-Gt(ROSA)26Sortm1(cre/Esr1)Nat/J |
| Stock Number: | 004847 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Notice Regarding cre/Esr1 (Tamoxifen Inducible Cre) Strains.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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