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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Former Names B6EiC3Sn-Rb(12.Ts171665Dn)2Cje/CjeDn (Changed: 16-NOV-11 ) B6EiC3Sn-Rb(12.T171665Dn)2Cje/CjeDn (Changed: 05-DEC-08 ) B6EiC3Sn-Rb(12.Ts171665Dn)2Cje/CjeDn (Changed: 09-MAY-08 ) B6EiC3Sn-Rb(12.Ts65Dn)1Cje (Changed: 29-MAR-05 ) Type Chromosome Aberration; Robertsonian; Trisomy; Additional information on Mice with Chromosomal Aberrations. Type Mutant Stock; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Rb(12.Ts171665Dn)2Cje female x B6EiC3SnF1/J (001875) male or reciprocal Species laboratory mouse Generation F1 (04-JUN-10)
Generation DefinitionsAppearance
agouti or black
Related Genotype: A/A or A/a or a/aImportant Note
Pde6brd1, the recessive retinal degeneration 1 mutation, is segregating in this colony. Animals that are homozygous for rd1 will be blind.Description
The trisomic segment in the Rb(12.Ts171665Dn)2Cje strain is genetically identical to the Ts65Dn strains, Stock No. 001924 and Stock No. 005252, with trisomy of the Chr 16 genes, from Mrpl39 to the distal telomere. Quantitative PCR confirmed the triplication of Chr 16 genes from App to Mx1. Transmission of the chromosome 16 segmental trisomy through the female germline is significantly improved over Ts65Dn (43% versus 24%). Dendritic spines on granule cells in the fascia dentata are enlarged in size and decreased in density (Villar AJ, et al. 2005). Unlike Ts65Dn, males are fertile. Trisomic mice are 20% smaller in size than controls. This strain serves a model for Down syndrome.Development
The Rb(12.Ts171665Dn)2Cje strain was the result of a spontaneous fusion between the small Ts65Dn marker chromosome (carrying the distal end of Chr 16) and Chr 12, forming a Robertsonian fusion chromosome. This Robertsonian chromosome was found among the progeny of a mating between a trisomic Ts65Dn female, B6EiC3Sn a/A - Ts(1716)65Dn (Stock No. 001924) and a diploid B6EiC3SnF1 male. Quantitative PCR confirmed the triplication of Chr 16 genes from App to Mx1, the same as in Ts65Dn strains.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 001875 B6EiC3SnF1/J | ||
| Considerations for Choosing Controls | ||
Related Strains
Robertsonian Chromosome
View Robertsonian Chromosome (67 strains)
Phenotype
Phenotype Information
- Characteristics of this human disease are associated with transgenes and other mutation types in the mouse.
Down Syndrome
assigned by genotype
Rb(12.Ts171665Dn)2Cje/0
involves: C3H/HeSnJ * C57BL/6JEi
- growth/size phenotype
- decreased body size
- mice are ~20% smaller in size compared to normal littermates postnatally, which persists throughout life (MGI Ref ID J:96650)
- reproductive system phenotype
- *normal* reproductive system phenotype
- decreased testis weight
- testes are 65.6% of control testes weights (MGI Ref ID J:96650)
- nervous system phenotype
- abnormal hippocampus morphology
- basal calcium levels of hippocampal neurons in culture is elevated compared to controls (MGI Ref ID J:110478)
- loss of hippocampal neurons
- 50% of hippocampal neurons cultured in vitro die after 7 days compared to just 15% for controls (MGI Ref ID J:110478)
- abnormal neuron morphology
- spine density in dentate granule cells is decreased (by ~15%) compared to controls; decrease is significant for inner and middle third portions of the molecular layer of the fascia dentate (MGI Ref ID J:96650)
- dendritic spines are significantly enlarged, with ~13% having a head area larger than 0.5 um2 compared to 2% in controls; spines have significantly shorter necks, by ~30% compared to controls (MGI Ref ID J:96650)
- loss of hippocampal neurons
- 50% of hippocampal neurons cultured in vitro die after 7 days compared to just 15% for controls (MGI Ref ID J:110478)
- increased neuron apoptosis
- the number of apoptotic neurons in the cortex is more than double that of controls (MGI Ref ID J:110478)
- endocrine/exocrine gland phenotype
- decreased testis weight
- testes are 65.6% of control testes weights (MGI Ref ID J:96650)
- cellular phenotype
- increased neuron apoptosis
- the number of apoptotic neurons in the cortex is more than double that of controls (MGI Ref ID J:110478)
This mouse can be used to support research in many areas including:
Mouse/Human Gene Homologs
Down syndrome
Neurobiology Research
Down syndrome
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Rb(12.Ts171665Dn)2Cje | ||
|---|---|---|---|
| Allele Name | Robertsonian translocation, Chr 12 and T17<16>Dn, Charles J Epstein 2 | ||
| Allele Type | Not Applicable | ||
| Common Name(s) | Rb2Cje; Ts(Rb(12.1716))2Cje; Ts2Cje; | ||
| Gene Symbol and Name | Rb(12.Ts171665Dn)2Cje, Robertsonian translocation, Chr 12 and T17<16>Dn, Charles J Epstein 2 | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Rb(12.T171665Dn)2Cje; Ts(Rb(12.1716))2Cje; | ||
| Molecular Note | A female mouse with 40 chromosomes and 41 arms was detected by chromosome analysis of progeny from T171665Dn mice. This mouse had a unique biarmed chromosome that appeared to be the result of a fusion between chromosome 12 and the marker chromosome T(1716)65Dn. | ||
Genotyping
Genotyping Information
Genotyping Protocols
Ts1716, Melt Curve Analysis
Generic Pde6b, Melt Curve Analysis
Generic Pde6b, Standard PCR
Trisomy QPCR, QPCR
Ts(1716), Separated PCR
Ts1716, High Resolution Melting
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Villar AJ; Belichenko PV; Gillespie AM; Kozy HM; Mobley WC; Epstein CJ. 2005. Identification and characterization of a new Down syndrome model, Ts[Rb(12.1716)]2Cje, resulting from a spontaneous Robertsonian fusion between T(171)65Dn and mouse chromosome 12. Mamm Genome 16(2):79-90. [PubMed: 15859352] [MGI Ref ID J:96650]
Additional References
Rb(12.Ts171665Dn)2Cje relatedDorsey SG; Renn CL; Carim-Todd L; Barrick CA; Bambrick L; Krueger BK; Ward CW; Tessarollo L. 2006. In vivo restoration of physiological levels of truncated TrkB.T1 receptor rescues neuronal cell death in a trisomic mouse model. Neuron 51(1):21-8. [PubMed: 16815329] [MGI Ref ID J:110478]
Ishihara K; Amano K; Takaki E; Ebrahim AS; Shimohata A; Shibazaki N; Inoue I; Takaki M; Ueda Y; Sago H; Epstein CJ; Yamakawa K. 2009. Increased lipid peroxidation in Down's syndrome mouse models. J Neurochem 110(6):1965-76. [PubMed: 19645748] [MGI Ref ID J:152486]
Ishihara K; Amano K; Takaki E; Shimohata A; Sago H; Epstein CJ; Yamakawa K. 2010. Enlarged brain ventricles and impaired neurogenesis in the Ts1Cje and Ts2Cje mouse models of Down syndrome. Cereb Cortex 20(5):1131-43. [PubMed: 19710359] [MGI Ref ID J:174165]
Levine S; Saltzman A; Levy E; Ginsberg SD. 2009. Systemic pathology in aged mouse models of Down's syndrome and Alzheimer's disease. Exp Mol Pathol 86(1):18-22. [PubMed: 19041304] [MGI Ref ID J:174270]
Rachidi M; Lopes C. 2007. Mental retardation in Down syndrome: from gene dosage imbalance to molecular and cellular mechanisms. Neurosci Res 59(4):349-69. [PubMed: 17897742] [MGI Ref ID J:128743]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Mating System Rb(12.Ts171665Dn)2Cje female x B6EiC3SnF1/J (001875) male or reciprocal Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Trisomic for distal Chromosome 16
Price per Pair (US dollars $) Pair Genotype $342.00 B6EiC3SnF1/J (001875) x Trisomic for distal Chromosome 16 $342.00 Trisomic for distal Chromosome 16 x B6EiC3SnF1/J (001875) Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Trisomic for distal Chromosome 16
Price per Pair (US dollars $) Pair Genotype $444.60 B6EiC3SnF1/J (001875) x Trisomic for distal Chromosome 16 $444.60 Trisomic for distal Chromosome 16 x B6EiC3SnF1/J (001875) Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- View the complete collection of spontaneous mutants in the Mouse Mutant Resource.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
- A 10% price increase applied to orders for this strain placed by for-profit companies.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 001875 B6EiC3SnF1/J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Important Note
Pde6brd1, the recessive retinal degeneration 1 mutation, is segregating in this colony. Animals that are homozygous for rd1 will be blind.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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