Strain Name:

B6EiC3Sn-Rb(12.Ts171665Dn)2Cje/CjeDnJ

Stock Number:

004850

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Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
This strain carries the same trisomic segment as other strains that are models for Down syndrome, but may have advantages in that transmission of the trisomic segment through the female germline is greater and males are fertile.

Description

Strain Information

Former Names B6EiC3Sn-Rb(12.Ts171665Dn)2Cje/CjeDn    (Changed: 16-NOV-11 )
B6EiC3Sn-Rb(12.T171665Dn)2Cje/CjeDn    (Changed: 05-DEC-08 )
B6EiC3Sn-Rb(12.Ts171665Dn)2Cje/CjeDn    (Changed: 09-MAY-08 )
B6EiC3Sn-Rb(12.Ts65Dn)1Cje    (Changed: 29-MAR-05 )
Type Chromosome Aberration; Robertsonian; Trisomy;
Additional information on Mice with Chromosomal Aberrations.
Type Mutant Stock;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Mating SystemRb(12.Ts171665Dn)2Cje female x B6EiC3SnF1/J (001875) male or reciprocal
Specieslaboratory mouse
GenerationF1 (04-JUN-10)
Generation Definitions

Appearance
agouti or black
Related Genotype: A/A or A/a or a/a

Important Note
Pde6brd1, the recessive retinal degeneration 1 mutation, is segregating in this colony. Animals that are homozygous for rd1 will be blind.

Description
The trisomic segment in the Rb(12.Ts171665Dn)2Cje strain is genetically identical to the Ts65Dn strains, Stock No. 001924 and Stock No. 005252, with trisomy of the Chr 16 genes, from Mrpl39 to the distal telomere. Quantitative PCR confirmed the triplication of Chr 16 genes from App to Mx1. Transmission of the chromosome 16 segmental trisomy through the female germline is significantly improved over Ts65Dn (43% versus 24%). Dendritic spines on granule cells in the fascia dentata are enlarged in size and decreased in density (Villar AJ, et al. 2005). Unlike Ts65Dn, males are fertile. Trisomic mice are 20% smaller in size than controls. This strain serves a model for Down syndrome.

Development
The Rb(12.Ts171665Dn)2Cje strain was the result of a spontaneous fusion between the small Ts65Dn marker chromosome (carrying the distal end of Chr 16) and Chr 12, forming a Robertsonian fusion chromosome. This Robertsonian chromosome was found among the progeny of a mating between a trisomic Ts65Dn female, B6EiC3Sn a/A - Ts(1716)65Dn (Stock No. 001924) and a diploid B6EiC3SnF1 male. Quantitative PCR confirmed the triplication of Chr 16 genes from App to Mx1, the same as in Ts65Dn strains.

Control Information

  Control
   Wild-type from the colony
   001875 B6EiC3SnF1/J
 
  Considerations for Choosing Controls

Related Strains

Robertsonian Chromosome
001099   AEJ/GnRk-Rb(11.14)1Dn/J
002545   B6 x RBA/DnJ
000610   B6 x STOCK Rb(6.15)1Ald/J
000861   B6.Cg-Rb(1.3)1Lub/J
000952   B6.Cg-Rb(10.11)8Bnr/J
000960   B6.Cg-Rb(2.8)2Lub/J
001733   B6.WMP-Rb(10.17)9Mpl/J
001734   B6.WMP-Rb(13.15)10Mpl/J
001727   B6.WMP-Rb(2.16)3Mpl/J
001728   B6.WMP-Rb(3.12)4Mpl/J
001729   B6.WMP-Rb(4.6)5Mpl/J
001732   B6.WMP-Rb(8.9)8Mpl/J
001796   B6Ei.Cg-Rb(1.2)18Lub/J
001921   B6Ei.Cg-Rb(1.7)1Rma/J
001386   B6Ei.Cg-Rb(10.12)5Lub/J
001617   B6Ei.Cg-Rb(10.15)23Lub/J
001795   B6Ei.Cg-Rb(11.13)4Bnr/J
000150   B6Ei.Cg-Rb(11.16)2H/J
000906   B6Ei.Cg-Rb(16.17)8Lub tw3Lub/+ +/J
001387   B6Ei.Cg-Rb(2.17)11Rma/J
001613   B6Ei.Cg-Rb(5.14)21Lub/J
001616   B6Ei.Cg-Rb(5.15)4Lub/J
001791   B6Ei.Cg-Rb(5.19)1Wh/J
000762   B6Ei.Cg-Rb(6.15)1Ald/J
001798   B6Ei.Cg-Rb(6.16)24Lub/J
001389   B6Ei.Cg-Rb(6.7)13Rma/J
001615   B6Ei.Cg-Rb(7.18)9Lub/J
001797   B6Ei.Cg-Rb(8.12)22Lub/J
000888   B6Ei.Cg-Rb(8.14)16Rma/J
001792   B6Ei.Cg-Rb(9.19)163H/J
001794   B6Ei.WMP-Rb(13.16)1Mpl/J
002470   B6EiC3Sn x B6-Rb(5.8)3Dn/J
000975   C57BL/6J x STOCK Rb(1.3)1Ei/J
001391   C57BL/6J-Rb(4.15)1Rk/J
001390   C57BL/6J-Rb(6.18)2Dn/J
001793   C57BL/6JEi-Rb(X.2)2Ad/J
000714   CByJ.RBF-Rb(8.12)5Bnr
001802   CByJ.RBF-Rb(8.12)5Bnr/J
000609   RBB/DnJ
000889   RBC/DnJ
000862   STOCK Rb(1.7)1Rma/J
000891   STOCK Rb(10.11)5Rma/J
000614   STOCK Rb(10.11)8Bnr/J
000892   STOCK Rb(10.12)5Lub/J
000729   STOCK Rb(11.13)4Bnr/J
000895   STOCK Rb(11.13)6Lub/J
001100   STOCK Rb(13.16)1Mpl/J
000615   STOCK Rb(16.17)7Bnr
003304   STOCK Rb(16.17)7Bnr-Myo15sh2-2J/J
000898   STOCK Rb(16.17)8Lub/J
000865   STOCK Rb(2.17)11Rma/J
000867   STOCK Rb(2.18)6Rma/J
000871   STOCK Rb(3.15)2Rk/J
000980   STOCK Rb(3.8)2Ei/J
000869   STOCK Rb(3.8)2Rma/J
000870   STOCK Rb(3.9)12Lub/J
000873   STOCK Rb(4.11)12Rma/J
000876   STOCK Rb(4.15)4Rma/J
000720   STOCK Rb(4.6)2Bnr/J
000878   STOCK Rb(5.14)21Lub/J
000880   STOCK Rb(5.15)15Rma/J
000881   STOCK Rb(5.17)7Rma/J
000882   STOCK Rb(5.19)1Wh/J
001884   STOCK Rb(5.6)1Ty/J
000885   STOCK Rb(6.16)24Lub
000887   STOCK Rb(8.12)22Lub/J
000613   STOCK Rb(9.19)163H/J
View Robertsonian Chromosome     (67 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Characteristics of this human disease are associated with transgenes and other mutation types in the mouse.
Down Syndrome
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Rb(12.Ts171665Dn)2Cje/0

        involves: C3H/HeSnJ * C57BL/6JEi
  • growth/size/body phenotype
  • decreased body size
    • mice are ~20% smaller in size compared to normal littermates postnatally, which persists throughout life   (MGI Ref ID J:96650)
  • reproductive system phenotype
  • *normal* reproductive system phenotype
    • mice are viable and fertile   (MGI Ref ID J:96650)
    • testes are structurally indistinguishable from controls and have normal progression of spermatogenesis   (MGI Ref ID J:96650)
    • decreased testis weight
      • testes are 65.6% of control testes weights   (MGI Ref ID J:96650)
  • nervous system phenotype
  • abnormal hippocampus morphology
    • basal calcium levels of hippocampal neurons in culture is elevated compared to controls   (MGI Ref ID J:110478)
    • loss of hippocampal neurons
      • 50% of hippocampal neurons cultured in vitro die after 7 days compared to just 15% for controls   (MGI Ref ID J:110478)
  • abnormal neuron morphology
    • spine density in dentate granule cells is decreased (by ~15%) compared to controls; decrease is significant for inner and middle third portions of the molecular layer of the fascia dentate   (MGI Ref ID J:96650)
    • dendritic spines are significantly enlarged, with ~13% having a head area larger than 0.5 um2 compared to 2% in controls; spines have significantly shorter necks, by ~30% compared to controls   (MGI Ref ID J:96650)
    • loss of hippocampal neurons
      • 50% of hippocampal neurons cultured in vitro die after 7 days compared to just 15% for controls   (MGI Ref ID J:110478)
  • increased neuron apoptosis
    • the number of apoptotic neurons in the cortex is more than double that of controls   (MGI Ref ID J:110478)
  • endocrine/exocrine gland phenotype
  • decreased testis weight
    • testes are 65.6% of control testes weights   (MGI Ref ID J:96650)
  • cellular phenotype
  • increased neuron apoptosis
    • the number of apoptotic neurons in the cortex is more than double that of controls   (MGI Ref ID J:110478)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Down syndrome

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Rb(12.Ts171665Dn)2Cje
Allele Name Robertsonian translocation, Chr 12 and T17<16>Dn, Charles J Epstein 2
Allele Type Not Applicable
Common Name(s) Rb2Cje; Ts(Rb(12.1716))2Cje; Ts2Cje;
Gene Symbol and Name Rb(12.Ts171665Dn)2Cje, Robertsonian translocation, Chr 12 and T17<16>Dn, Charles J Epstein 2
Chromosome UN
Gene Common Name(s) Rb(12.T171665Dn)2Cje; Ts(Rb(12.1716))2Cje;
Molecular Note A female mouse with 40 chromosomes and 41 arms was detected by chromosome analysis of progeny from T171665Dn mice. This mouse had a unique biarmed chromosome that appeared to be the result of a fusion between chromosome 12 and the marker chromosome T(1716)65Dn.

Genotyping

Genotyping Information

Genotyping Protocols

Ts1716, Melt Curve Analysis
Generic Pde6b, High Resolution Melting
Generic Pde6b, Standard PCR
Trisomy QPCR, QPCR
Ts(1716), Separated PCR
Ts1716, High Resolution Melting


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Villar AJ; Belichenko PV; Gillespie AM; Kozy HM; Mobley WC; Epstein CJ. 2005. Identification and characterization of a new Down syndrome model, Ts[Rb(12.1716)]2Cje, resulting from a spontaneous Robertsonian fusion between T(171)65Dn and mouse chromosome 12. Mamm Genome 16(2):79-90. [PubMed: 15859352]  [MGI Ref ID J:96650]

Additional References

Rb(12.Ts171665Dn)2Cje related

Dorsey SG; Renn CL; Carim-Todd L; Barrick CA; Bambrick L; Krueger BK; Ward CW; Tessarollo L. 2006. In vivo restoration of physiological levels of truncated TrkB.T1 receptor rescues neuronal cell death in a trisomic mouse model. Neuron 51(1):21-8. [PubMed: 16815329]  [MGI Ref ID J:110478]

Ishihara K; Amano K; Takaki E; Ebrahim AS; Shimohata A; Shibazaki N; Inoue I; Takaki M; Ueda Y; Sago H; Epstein CJ; Yamakawa K. 2009. Increased lipid peroxidation in Down's syndrome mouse models. J Neurochem 110(6):1965-76. [PubMed: 19645748]  [MGI Ref ID J:152486]

Ishihara K; Amano K; Takaki E; Shimohata A; Sago H; Epstein CJ; Yamakawa K. 2010. Enlarged brain ventricles and impaired neurogenesis in the Ts1Cje and Ts2Cje mouse models of Down syndrome. Cereb Cortex 20(5):1131-43. [PubMed: 19710359]  [MGI Ref ID J:174165]

Levine S; Saltzman A; Levy E; Ginsberg SD. 2009. Systemic pathology in aged mouse models of Down's syndrome and Alzheimer's disease. Exp Mol Pathol 86(1):18-22. [PubMed: 19041304]  [MGI Ref ID J:174270]

Rachidi M; Lopes C. 2007. Mental retardation in Down syndrome: from gene dosage imbalance to molecular and cellular mechanisms. Neurosci Res 59(4):349-69. [PubMed: 17897742]  [MGI Ref ID J:128743]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX11

Colony Maintenance

Mating SystemRb(12.Ts171665Dn)2Cje female x B6EiC3SnF1/J (001875) male or reciprocal
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleTrisomic for distal Chromosome 16  
Price per Pair (US dollars $)Pair Genotype
$357.00Trisomic for distal Chromosome 16 x B6EiC3SnF1/J (001875)  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleTrisomic for distal Chromosome 16  
Price per Pair (US dollars $)Pair Genotype
$464.10Trisomic for distal Chromosome 16 x B6EiC3SnF1/J (001875)  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

General Supply Notes

  • A 10% price increase applied to orders for this strain placed by for-profit companies.

Control Information

  Control
   Wild-type from the colony
   001875 B6EiC3SnF1/J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Important Note

Pde6brd1, the recessive retinal degeneration 1 mutation, is segregating in this colony. Animals that are homozygous for rd1 will be blind.

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