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Strain Name:

B6;129-Crb1rd8/J

Stock Number:

004852

Availability:

Repository-Cryopreserved

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General Terms and Conditions

Former Name      B6;129-Crb1rd8-nmf144/J    (Changed: 15-DEC-04 )
      NMF144    (Changed: 15-DEC-04 )
Genes & Alleles   Crb1;   Crb1rd8;

Product Information

Strain Details

Type JAX® GEMM® Strain - Chemically Induced Mutation
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Coisogenic
Type JAX® GEMM® Strain - Mutant Strain
Specieslaboratory mouse
Background Strain C57BL/6J
Donor Strain 129
H2 Haplotypeb
GenerationN3F2N1p (23-JUL-04)

Strain Description
View strain phenotype and additional information on the Neuroscience Mutagenesis Facility web page for Crb1rd8 (Crb1nmf144) entry.

Mammalian Phenotype Terms assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Crb1rd8/Crb1rd8

        STOCK Crb1rd8/J
  • vision/eye phenotype
  • abnormal ocular fundus morphology (J:85459)
    • mice exhibit large, irregularly shaped spots concentrated in the inferior nasal quadrant of the fundus as early as week 3 that correspond to retinal folds and pseudorosettes
    • abnormal retina morphology (J:85459)
      • retinal thinning in both the inner and outer segment is observed
      • the external limiting membrane in the retina is fragmented and disorganized at 2 weeks and is more severe at 4 weeks
      • abnormal Muller cell morphology (J:85459)
        • Muller cells exhibit increased electron density and cytoplasmic strands that are more easily traced through the plexiform layer than in wild type mice
      • abnormal retinal photoreceptor morphology (J:85459)
        • the photoreceptor lamellae breaks down
        • abnormal photoreceptor inner segment morphology (J:85459)
          • the photoreceptor inner segments lose their orderly arrangement
          • by 5 months the inner segment approaches the retinal pigment epithelium
          • swelling occurs in portions of the inner segments
          • short photoreceptor inner segment (J:85459)
            • at 4 weeks the photoreceptor inner segments are 25% shorter
        • decreased retinal photoreceptor cell number (J:85459)
          • in the region of focal degeneration photoreceptors are lost
        • photoreceptor outer segment degeneration (J:85459)
          • by week 10 the outer segment begins to fragment
          • by 5 months only a few outer segment fragments remain
        • short photoreceptor outer segment (J:85459)
          • the photoreceptor outer segments are shortened
      • thin retinal outer nuclear layer (J:85459)
        • in older mice, the retinal outer nuclear layer is reduced to a single row of nuclei
  • nervous system phenotype
  • abnormal Muller cell morphology (J:85459)
    • Muller cells exhibit increased electron density and cytoplasmic strands that are more easily traced through the plexiform layer than in wild type mice
  • abnormal retinal photoreceptor morphology (J:85459)
    • the photoreceptor lamellae breaks down
    • abnormal photoreceptor inner segment morphology (J:85459)
      • the photoreceptor inner segments lose their orderly arrangement
      • by 5 months the inner segment approaches the retinal pigment epithelium
      • swelling occurs in portions of the inner segments
      • short photoreceptor inner segment (J:85459)
        • at 4 weeks the photoreceptor inner segments are 25% shorter
    • decreased retinal photoreceptor cell number (J:85459)
      • in the region of focal degeneration photoreceptors are lost
    • photoreceptor outer segment degeneration (J:85459)
      • by week 10 the outer segment begins to fragment
      • by 5 months only a few outer segment fragments remain
    • short photoreceptor outer segment (J:85459)
      • the photoreceptor outer segments are shortened

Crb1rd8/Crb1rd8

        involves: C3HfB6/Ga * C57BL/6 * CAST/EiJ
  • vision/eye phenotype
  • abnormal retina morphology (J:85459)
    • 19% of mice do not display the spotting phenotype on a CAST/EiJ mixed background

Crb1rd8/Crb1rd8

        B6.Cg-Crb1rd8
  • vision/eye phenotype
  • *normal* vision/eye phenotype (J:85459)
    • mice no longer display the spotting phenotype when backcrossed from a CAST/EiJ background onto a C57BL/6 background

Gene & Allele Details

Allele Symbol Crb1rd8
Allele Name retinal degeneration 8
Common Name(s) nmf144;
Strain of OriginC57BL/6J
Gene Symbol and Name Crb1, crumbs homolog 1 (Drosophila)
Chromosome 1
Gene Common Name(s) 7530426H14Rik; A930008G09Rik; LCA8; RIKEN cDNA 7530426H14 gene; RIKEN cDNA A930008G09 gene; RP12;
Molecular Note The mutation in the rd8 mouse has been identified as a single base deletion in the Crb1 gene. This deletion causes a frame shift and a premature stop codon that truncates the transmembrane and cytoplasmic domain of the protein. [J:85459]

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Crb1rd8 allele
003392   STOCK Crb1rd8/J
View Strains carrying   Crb1rd8     (1 strain)

Research Applications

This mouse can be used to support research in many areas including:

Neurobiology Research
Neuroscience Mutagenesis Facility Strain

Crb1rd8 related

Cell Biology Research
Defects in Cell Adhesion Molecules

Developmental Biology Research
Eye Defects

Mouse/Human Gene Homologs
Retinitis pigmentosa 12; Leber congenital amaurosis (CLA)

Sensorineural Research
Retinal Degeneration

References

Selected Reference(s)

Mehalow AK; Kameya S; Smith RS; Hawes NL; Denegre JM; Young JA; Bechtold L; Haider NB; Tepass U; Heckenlively JR; Chang B; Naggert JK; Nishina PM. 2003. CRB1 is essential for external limiting membrane integrity and photoreceptor morphogenesis in the mammalian retina. Hum Mol Genet 12(17):2179-89. [PubMed: 12915475]  [J:85459]

Additional References

Price and Supply Information

Strain Name: B6;129-Crb1rd8/J
Stock Number: 004852

Price Details

IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.

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Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to the Supply Notes for further information.
Supply Notes Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
This strain is included in the Neuroscience Mutagenesis Facility (NMF).

LicensingSee General Terms and Conditions below  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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