Description

Strain Information

Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse   Donating Investigator Douglas Grossman,   University of Utah

Description
These transgenic mice express the mouse baculoviral IAP repeat-containing 5, Birc5 (survivin), gene under the direction of the human keratin 14 promoter. Transgene expression is specific to epidermal and follicular keratinocytes. Mice hemizygous for the transgenic insert are resistant to chemical (DMBA)- and UVB-induced keratinocyte apoptosis in vivo. Although hemizygotes were less susceptible to DMBA-induced papilloma formation, spontaneous papilloma regression was not observed and there was enhanced conversion of papillomas to carcinomas. This mutant mouse strain represents a model that may be useful in studies of keratinocyte apoptosis and skin cancer development.

Development
A transgenic construct containing a 2.1kb sequence of human keratin 14 promoter upstream of the mouse baculoviral IAP repeat-containing 5 (Birc5) gene and a 2.2kb sequence of the human growth hormone and polyadenylation sequence was injected into fertilized B6C3F2 mouse eggs.

Control Information

	Control
	Noncarrier female
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of KRT14

006403	129S.B6-Tg(KRT14-Esr1/HRAS)1Pkha/J
006661	129S.B6-Tg(KRT14-RAF1/ESR1)1Pkha/J
007622	B6;SJL-Tg(KRT14-Hr)551Cct/J
007678	B6;SJL-Tg(KRT14-rtTA)208Jek/J
002980	C57BL/6-Tg(Cd152Ig)1Jbs/J
006822	FVB-Tg(KRT14-MAP2K1/Esr1)12Pkha/J
005705	FVB-Tg(KRT14-Vegfa)3Dtm/J
008099	FVB-Tg(KRT14-rtTA)F42Efu/J
004782	STOCK Tg(KRT14-cre)1Amc/J
005107	STOCK Tg(KRT14-cre/Esr1)20Efu/J

View Strains carrying other alleles of KRT14     (10 strains)

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Apoptosis Research
Endogenous Regulators
Extracellular Modulators

Cancer Research
Genes Regulating Growth and Proliferation
Increased Tumor Incidence (Skin Cancers: Induced)
Increased Tumor Incidence (Skin Cancers: Resistant)
Oncogenes
Tumor Resistance

Cell Biology Research
Genes Regulating Growth and Proliferation

Genes & Alleles

Gene & Allele Information

Allele Symbol		Tg(KRT14-Birc5)19Gros
Allele Name		transgene insertion 19, Doug Grossman
Allele Type		Transgenic (random, expressed)
Common Name(s)		K14-Survivin;
Mutation Made By		Douglas Grossman,   University of Utah
Strain of Origin		(C57BL/6 x C3H)F2
Expressed Gene		Birc5, baculoviral IAP repeat-containing 5, mouse, laboratory
Promoter		KRT14, keratin 14, human
Molecular Note		The transgene contains a 2.1kb sequence of human keratin 14 promoter upstream of the mouse baculoviral IAP repeat-containing 5 (Birc5) gene and a 2.2kb sequence of the human growth hormone and polyadenylation sequence. Transgene expression is specific to epidermal and follicular keratinocytes. Five founder mice were established and line 19 was chosen as the representative line.

Genotyping

Genotyping Information

Genotyping Protocols

Tg(KRT14-Birc5)19Gros, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Grossman D; Kim PJ; Blanc-Brude OP; Brash DE; Tognin S; Marchisio PC; Altieri DC. 2001. Transgenic expression of survivin in keratinocytes counteracts UVB-induced apoptosis and cooperates with loss of p53. J Clin Invest 108(7):991-9. [PubMed: 11581300]  [MGI Ref ID J:93483]

Additional References

Allen SM; Florell SR; Hanks AN; Alexander A; Diedrich MJ; Altieri DC; Grossman D. 2003. Survivin expression in mouse skin prevents papilloma regression and promotes chemical-induced tumor progression. Cancer Res 63(3):567-72. [PubMed: 12566297]  [MGI Ref ID J:81530]

Tg(KRT14-Birc5)19Gros related

Zhang W; Hanks AN; Boucher K; Florell SR; Allen SM; Alexander A; Brash DE; Grossman D. 2005. UVB-induced apoptosis drives clonal expansion during skin tumor development. Carcinogenesis 26(1):249-57. [PubMed: 15498793]  [MGI Ref ID J:95011]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & Husbandry	The resulting chimeric animals were crossed to C57BL/6NCR mice, and then backcrossed to the same for 5 generations. The strain is maintained as a hemizygote.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	Noncarrier female
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering and Purchasing Information

      Purchasing Information
      JAX^® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.