Strain Name:

B6.129X-Mmp12tm1Sds/J

Stock Number:

004855

Order this mouse

Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
Mice homozygous for the knock-out allele exhibit abnormal macrophage physiology, decreased sensitivity to cigarette smoke, reduced IL-13 induced tissue inflammation, and decreased littler size. This mutant mouse strain may be useful in studies of wound repair and extracellular matrix remodeling.

Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Mating SystemHomozygote x Homozygote         (Female x Male)   01-MAR-06
Specieslaboratory mouse
GenerationN10+N4F8 (11-MAY-11)
Generation Definitions
 
Donating Investigator Steven D Shapiro,   University of Pittsburgh

Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygous females have reduced litter sizes (~60% of wildtype). No gene product (mRNA or protein) is detected by Northern or Western blot analysis of peritoneal macrophages and fluid. Casein substrate gel zymographic analysis of thioglycollate-stimulated cultured macrophages and peritoneal fluid reveals there is no lytic activity. In whole lung extracts, no elastase activity was detected by zymography. Immunohistochemistry of lung tissue does not detect macrophage elastase. Macrophages from homozygous mice are unable to penetrate artificial basement membrane matrix. Addition of plasminogen to cultured macrophages does not increase elastase activity. When challenged with chronic exposure to cigarette smoke, mutant mice do not develop emphysema. IL-13 induced tissue inflammation is reduced in homozygotes. This mutant mouse strain may be useful in studies of wound repair and extracellular matrix remodeling.

Development
A targeting vector containing a neomycin resistance gene driven by the mouse phosphoglycerate kinase promoter was used to disrupt most of exon 2 of the targeted gene. The construct was electroporated into 129X1/SvJ derived RW4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice to establish the colony. The donating investigator reported to have backcrossed the colony to C57BL/6J for ten generations (see SNP note below), and then bred homozygous mice together for several generations prior to sending to The Jackson Laboratory Repository in 2004. In 2007, a single nucleotide panel (SNP) analysis of the living colony revealed the mice may not have been completely backcrossed onto the C57BL/6J genetic background. The colony was then backcrossed onto C57BL/6J for at least three additional generations. In 2010, a 32 SNP panel analysis covering all chromosomes showed all markers were C57BL/6 allele-type.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Mmp12tm1Sds/Mmp12tm1Sds

        B6.129X1-Mmp12tm1Sds
  • homeostasis/metabolism phenotype
  • decreased physiological sensitivity to xenobiotic
    • mice fail to develop emphysema following a 6 month exposure to cigarette smoke unlike wild-type mice   (MGI Ref ID J:86599)
    • following a 6 month exposure to cigarette smoke, mice exhibit fewer macrophages in bronchoalveolar lavage samples compared to wild-type mice   (MGI Ref ID J:86599)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Mmp12tm1Sds/Mmp12tm1Sds

        involves: 129X1/SvJ
  • immune system phenotype
  • abnormal macrophage physiology
    • macrophages have impaired capacity to degrade elastin and cannot penetrate reconstituted basement membranes in vitro and in vivo   (MGI Ref ID J:32902)
  • reproductive system phenotype
  • decreased litter size
    • have litter sizes only 60% that of wildtype   (MGI Ref ID J:32902)
  • nervous system phenotype
  • abnormal myelination
    • exhibit decreased myelination in the corpus callosum at P7 and P10, but not at P14, as evidenced by decreased MBP expression   (MGI Ref ID J:105765)
  • decreased oligodendrocyte number
    • exhibit a decrease in the number of mature oligondendrocytes at P10, however no differences in oligodendrocyte precursor cell numbers   (MGI Ref ID J:105765)
  • homeostasis/metabolism phenotype
  • *normal* homeostasis/metabolism phenotype
    • mice exhibit normal susceptibility to induced aortic aneurysms   (MGI Ref ID J:62760)
  • hematopoietic system phenotype
  • abnormal macrophage physiology
    • macrophages have impaired capacity to degrade elastin and cannot penetrate reconstituted basement membranes in vitro and in vivo   (MGI Ref ID J:32902)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Mmp12tm1Sds related

Immunology, Inflammation and Autoimmunity Research
Inflammation

Metabolism Research
Enzyme Deficiency

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Mmp12tm1Sds
Allele Name targeted mutation 1, Steven D Shapiro
Allele Type Targeted (Null/Knockout)
Common Name(s) MME-; MMP-12-; Mmp12-;
Mutation Made By Emily Heiss,   Brigham and Women's Hospital
Strain of Origin129X1/SvJ
ES Cell Line NameRW-4
ES Cell Line Strain129X1/SvJ
Gene Symbol and Name Mmp12, matrix metallopeptidase 12
Chromosome 9
Gene Common Name(s) AV378681; HME; ME; MME; MMP-12; Mmel; expressed sequence AV378681; macrophage elastase; macrophage metalloelastase;
Molecular Note The gene was disrupted by replacement of the majority of exon 2 with a PGK-neo cassette via homologous recombination. Gene transcription and protein production was absent in homozygous mutant animals as demonstrated by Northern and Western blot analysis of peritoneal macrophages and peritoneal fluid. [MGI Ref ID J:32902]

Genotyping

Genotyping Information

Genotyping Protocols

Mmp12tm1Sdsalternate 1,

Separated MCA


Mmp12tm1Sdsalternate1, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Shipley JM; Wesselschmidt RL; Kobayashi DK; Ley TJ; Shapiro SD. 1996. Metalloelastase is required for macrophage-mediated proteolysis and matrix invasion in mice. Proc Natl Acad Sci U S A 93(9):3942-6. [PubMed: 8632994]  [MGI Ref ID J:32902]

Additional References

Carmeliet P; Moons L; Lijnen R; Baes M; Lemaitre V; Tipping P; Drew A; Eeckhout Y; Shapiro S; Lupu F; Collen D. 1997. Urokinase-generated plasmin activates matrix metalloproteinases during aneurysm formation. Nat Genet 17(4):439-44. [PubMed: 9398846]  [MGI Ref ID J:44387]

Hautamaki RD; Kobayashi DK; Senior RM; Shapiro SD. 1997. Requirement for macrophage elastase for cigarette smoke-induced emphysema in mice. Science 277(5334):2002-4. [PubMed: 9302297]  [MGI Ref ID J:43130]

Mmp12tm1Sds related

Baldo G; Wu S; Howe RA; Ramamoothy M; Knutsen RH; Fang J; Mecham RP; Liu Y; Wu X; Atkinson JP; Ponder KP. 2011. Pathogenesis of aortic dilatation in mucopolysaccharidosis VII mice may involve complement activation. Mol Genet Metab 104(4):608-19. [PubMed: 21944884]  [MGI Ref ID J:178881]

Bauters D; Van Hul M; Lijnen HR. 2013. Macrophage elastase (MMP-12) in expanding murine adipose tissue. Biochim Biophys Acta 1830(4):2954-9. [PubMed: 23295969]  [MGI Ref ID J:199049]

Carmeliet P; Moons L; Lijnen R; Baes M; Lemaitre V; Tipping P; Drew A; Eeckhout Y; Shapiro S; Lupu F; Collen D. 1997. Urokinase-generated plasmin activates matrix metalloproteinases during aneurysm formation. Nat Genet 17(4):439-44. [PubMed: 9398846]  [MGI Ref ID J:44387]

Churg A; Dai J; Zay K; Karsan A; Hendricks R; Yee C; Martin R; MacKenzie R; Xie C; Zhang L; Shapiro S; Wright JL. 2001. Alpha-1-antitrypsin and a broad spectrum metalloprotease inhibitor, RS113456, have similar acute anti-inflammatory effects. Lab Invest 81(8):1119-31. [PubMed: 11502863]  [MGI Ref ID J:71105]

Churg A; Zay K; Shay S; Xie C; Shapiro SD; Hendricks R; Wright JL. 2002. Acute cigarette smoke-induced connective tissue breakdown requires both neutrophils and macrophage metalloelastase in mice. Am J Respir Cell Mol Biol 27(3):368-74. [PubMed: 12204900]  [MGI Ref ID J:113625]

Dean RA; Cox JH; Bellac CL; Doucet A; Starr AE; Overall CM. 2008. Macrophage-specific metalloelastase (MMP-12) truncates and inactivates ELR+ CXC chemokines and generates CCL2, -7, -8, and -13 antagonists: potential role of the macrophage in terminating polymorphonuclear leukocyte influx. Blood 112(8):3455-64. [PubMed: 18660381]  [MGI Ref ID J:140224]

Denis M; Cormier Y; Fournier M; Tardif J; Laviolette M. 1991. Tumor necrosis factor plays an essential role in determining hypersensitivity pneumonitis in a mouse model. Am J Respir Cell Mol Biol 5(5):477-83. [PubMed: 1931076]  [MGI Ref ID J:11522]

Edelstein C; Shapiro SD; Klezovitch O; Scanu AM. 1999. Macrophage metalloelastase, MMP-12, cleaves human apolipoprotein(a) in the linker region between kringles IV-4 and IV-5. Potential relevance to lipoprotein(a) biology. J Biol Chem 274(15):10019-23. [PubMed: 10187779]  [MGI Ref ID J:115221]

England KA; Price AP; Tram KV; Shapiro SD; Blazar BR; Panoskaltsis-Mortari A. 2011. Evidence for early fibrosis and increased airway resistance in bone marrow transplant recipient mice deficient in MMP12. Am J Physiol Lung Cell Mol Physiol 301(4):L519-26. [PubMed: 21784967]  [MGI Ref ID J:176273]

Goncalves DaSilva A; Liaw L; Yong VW. 2010. Cleavage of osteopontin by matrix metalloproteinase-12 modulates experimental autoimmune encephalomyelitis disease in C57BL/6 mice. Am J Pathol 177(3):1448-58. [PubMed: 20651245]  [MGI Ref ID J:163629]

Goncalves DaSilva A; Yong VW. 2009. Matrix metalloproteinase-12 deficiency worsens relapsing-remitting experimental autoimmune encephalomyelitis in association with cytokine and chemokine dysregulation. Am J Pathol 174(3):898-909. [PubMed: 19218336]  [MGI Ref ID J:146812]

Hautamaki RD; Kobayashi DK; Senior RM; Shapiro SD. 1997. Requirement for macrophage elastase for cigarette smoke-induced emphysema in mice. Science 277(5334):2002-4. [PubMed: 9302297]  [MGI Ref ID J:43130]

Heymans S; Luttun A; Nuyens D; Theilmeier G; Creemers E; Moons L; Dyspersin GD; Cleutjens JP; Shipley M; Angellilo A; Levi M; Nube O; Baker A; Keshet E; Lupu F; Herbert JM; Smits JF; Shapiro SD; Baes M; Borgers M; Collen D; Daemen MJ; Carmeliet P. 1999. Inhibition of plasminogen activators or matrix metalloproteinases prevents cardiac rupture but impairs therapeutic angiogenesis and causes cardiac failure. Nat Med 5(10):1135-42. [PubMed: 10502816]  [MGI Ref ID J:124004]

Houghton AM; Grisolano JL; Baumann ML; Kobayashi DK; Hautamaki RD; Nehring LC; Cornelius LA; Shapiro SD. 2006. Macrophage elastase (matrix metalloproteinase-12) suppresses growth of lung metastases. Cancer Res 66(12):6149-55. [PubMed: 16778188]  [MGI Ref ID J:110107]

Houghton AM; Hartzell WO; Robbins CS; Gomis-Ruth FX; Shapiro SD. 2009. Macrophage elastase kills bacteria within murine macrophages. Nature 460(7255):637-41. [PubMed: 19536155]  [MGI Ref ID J:150964]

Johnson JL; Dwivedi A; Somerville M; George SJ; Newby AC. 2011. Matrix metalloproteinase (MMP)-3 activates MMP-9 mediated vascular smooth muscle cell migration and neointima formation in mice. Arterioscler Thromb Vasc Biol 31(9):e35-44. [PubMed: 21719762]  [MGI Ref ID J:191851]

Johnson JL; George SJ; Newby AC; Jackson CL. 2005. Divergent effects of matrix metalloproteinases 3, 7, 9, and 12 on atherosclerotic plaque stability in mouse brachiocephalic arteries. Proc Natl Acad Sci U S A 102(43):15575-80. [PubMed: 16221765]  [MGI Ref ID J:102482]

Kang HR; Cho SJ; Lee CG; Homer RJ; Elias JA. 2007. Transforming growth factor (TGF)-beta1 stimulates pulmonary fibrosis and inflammation via a Bax-dependent, bid-activated pathway that involves matrix metalloproteinase-12. J Biol Chem 282(10):7723-32. [PubMed: 17209037]  [MGI Ref ID J:120886]

Kassim SY; Fu X; Liles WC; Shapiro SD; Parks WC; Heinecke JW. 2005. NADPH oxidase restrains the matrix metalloproteinase activity of macrophages. J Biol Chem 280(34):30201-5. [PubMed: 15983040]  [MGI Ref ID J:101039]

Lanone S; Zheng T; Zhu Z; Liu W; Lee CG; Ma B; Chen Q; Homer RJ; Wang J; Rabach LA; Rabach ME; Shipley JM; Shapiro SD; Senior RM; Elias JA. 2002. Overlapping and enzyme-specific contributions of matrix metalloproteinases-9 and -12 in IL-13-induced inflammation and remodeling. J Clin Invest 110(4):463-74. [PubMed: 12189240]  [MGI Ref ID J:78575]

Larsen PH; DaSilva AG; Conant K; Yong VW. 2006. Myelin formation during development of the CNS is delayed in matrix metalloproteinase-9 and -12 null mice. J Neurosci 26(8):2207-14. [PubMed: 16495447]  [MGI Ref ID J:105765]

Lee MM; Yoon BJ; Osiewicz K; Preston M; Bundy B; van Heeckeren AM; Werb Z; Soloway PD. 2005. Tissue inhibitor of metalloproteinase 1 regulates resistance to infection. Infect Immun 73(1):661-5. [PubMed: 15618213]  [MGI Ref ID J:94836]

Li J; Wang JJ; Peng Q; Chen C; Humphrey MB; Heinecke J; Zhang SX. 2012. Macrophage metalloelastase (MMP-12) deficiency mitigates retinal inflammation and pathological angiogenesis in ischemic retinopathy. PLoS One 7(12):e52699. [PubMed: 23285156]  [MGI Ref ID J:195748]

Liu Y; Zhang M; Hao W; Mihaljevic I; Liu X; Xie K; Walter S; Fassbender K. 2013. Matrix metalloproteinase-12 contributes to neuroinflammation in the aged brain. Neurobiol Aging 34(4):1231-9. [PubMed: 23159549]  [MGI Ref ID J:203379]

Madala SK; Pesce JT; Ramalingam TR; Wilson MS; Minnicozzi S; Cheever AW; Thompson RW; Mentink-Kane MM; Wynn TA. 2010. Matrix metalloproteinase 12-deficiency augments extracellular matrix degrading metalloproteinases and attenuates IL-13-dependent fibrosis. J Immunol 184(7):3955-63. [PubMed: 20181883]  [MGI Ref ID J:160097]

Marsland BJ; Kurrer M; Reissmann R; Harris NL; Kopf M. 2008. Nippostrongylus brasiliensis infection leads to the development of emphysema associated with the induction of alternatively activated macrophages. Eur J Immunol 38(2):479-88. [PubMed: 18203142]  [MGI Ref ID J:131355]

Matute-Bello G; Wurfel MM; Lee JS; Park DR; Frevert CW; Madtes DK; Shapiro SD; Martin TR. 2007. Essential role of MMP-12 in Fas-induced lung fibrosis. Am J Respir Cell Mol Biol 37(2):210-21. [PubMed: 17446527]  [MGI Ref ID J:138494]

Morris DG; Huang X; Kaminski N; Wang Y; Shapiro SD; Dolganov G; Glick A; Sheppard D. 2003. Loss of integrin alpha(v)beta6-mediated TGF-beta activation causes Mmp12-dependent emphysema. Nature 422(6928):169-73. [PubMed: 12634787]  [MGI Ref ID J:89578]

Nury C; Bregant S; Czarny B; Berthon F; Cassar-Lajeunesse E; Dive V. 2013. Detection of endogenous matrix metalloprotease-12 active form with a novel broad spectrum activity-based probe. J Biol Chem 288(8):5636-44. [PubMed: 23271741]  [MGI Ref ID J:195650]

Pouladi MA; Robbins CS; Swirski FK; Cundall M; McKenzie AN; Jordana M; Shapiro SD; Stampfli MR. 2004. Interleukin-13-dependent expression of matrix metalloproteinase-12 is required for the development of airway eosinophilia in mice. Am J Respir Cell Mol Biol 30(1):84-90. [PubMed: 12842850]  [MGI Ref ID J:95132]

Pyo R; Lee JK; Shipley JM; Curci JA; Mao D; Ziporin SJ; Ennis TL; Shapiro SD; Senior RM; Thompson RW. 2000. Targeted gene disruption of matrix metalloproteinase-9 (gelatinase B) suppresses development of experimental abdominal aortic aneurysms [see comments] J Clin Invest 105(11):1641-9. [PubMed: 10841523]  [MGI Ref ID J:62760]

Senft AP; Korfhagen TR; Whitsett JA; Shapiro SD; LeVine AM. 2005. Surfactant protein-D regulates soluble CD14 through matrix metalloproteinase-12. J Immunol 174(8):4953-9. [PubMed: 15814723]  [MGI Ref ID J:98168]

Shapiro SD; Goldstein NM; Houghton AM; Kobayashi DK; Kelley D; Belaaouaj A. 2003. Neutrophil elastase contributes to cigarette smoke-induced emphysema in mice. Am J Pathol 163(6):2329-35. [PubMed: 14633606]  [MGI Ref ID J:86599]

Sun K; Gan Y; Metzger DW. 2011. Analysis of murine genetic predisposition to pneumococcal infection reveals a critical role of alveolar macrophages in maintaining the sterility of the lower respiratory tract. Infect Immun 79(5):1842-7. [PubMed: 21321074]  [MGI Ref ID J:171962]

Tjwa M ; Moons L ; Verfaillie C ; Carmeliet P. 2009. Antibiotics may impair hematopoietic recovery after cytotoxic myeloablation. Blood 113(7):1608-9. [PubMed: 19221044]  [MGI Ref ID J:146614]

Wang Y; Ait-Oufella H; Herbin O; Bonnin P; Ramkhelawon B; Taleb S; Huang J; Offenstadt G; Combadiere C; Renia L; Johnson JL; Tharaux PL; Tedgui A; Mallat Z. 2010. TGF-beta activity protects against inflammatory aortic aneurysm progression and complications in angiotensin II-infused mice. J Clin Invest 120(2):422-32. [PubMed: 20101093]  [MGI Ref ID J:156667]

Warner RL; Lewis CS; Beltran L; Younkin EM; Varani J; Johnson KJ. 2001. The role of metalloelastase in immune complex-induced acute lung injury. Am J Pathol 158(6):2139-44. [PubMed: 11395391]  [MGI Ref ID J:114408]

Warner RL; Lukacs NW; Shapiro SD; Bhagarvathula N; Nerusu KC; Varani J; Johnson KJ. 2004. Role of metalloelastase in a model of allergic lung responses induced by cockroach allergen. Am J Pathol 165(6):1921-30. [PubMed: 15579436]  [MGI Ref ID J:109719]

Weaver A; Goncalves da Silva A; Nuttall RK; Edwards DR; Shapiro SD; Rivest S; Yong VW. 2005. An elevated matrix metalloproteinase (MMP) in an animal model of multiple sclerosis is protective by affecting Th1/Th2 polarization. FASEB J 19(12):1668-70. [PubMed: 16081501]  [MGI Ref ID J:102657]

Wells JE; Biernaskie J; Szymanska A; Larsen PH; Yong VW; Corbett D. 2005. Matrix metalloproteinase (MMP)-12 expression has a negative impact on sensorimotor function following intracerebral haemorrhage in mice. Eur J Neurosci 21(1):187-96. [PubMed: 15654856]  [MGI Ref ID J:100812]

Wells JE; Rice TK; Nuttall RK; Edwards DR; Zekki H; Rivest S; Yong VW. 2003. An adverse role for matrix metalloproteinase 12 after spinal cord injury in mice. J Neurosci 23(31):10107-15. [PubMed: 14602826]  [MGI Ref ID J:86556]

Zhang L; Ikegami M; Korfhagen TR; McCormack FX; Yoshida M; Senior RM; Shipley JM; Shapiro SD; Whitsett JA. 2006. Neither SP-A nor NH2-terminal domains of SP-A can substitute for SP-D in regulation of alveolar homeostasis. Am J Physiol Lung Cell Mol Physiol 291(2):L181-90. [PubMed: 16500946]  [MGI Ref ID J:121212]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX11

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice may be bred together. Homozygous females have reduced litter sizes (~60% of wildtype).
Mating SystemHomozygote x Homozygote         (Female x Male)   01-MAR-06
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $199.90Female or MaleHomozygous for Mmp12tm1Sds  
Price per Pair (US dollars $)Pair Genotype
$399.80Homozygous for Mmp12tm1Sds x Homozygous for Mmp12tm1Sds  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $259.90Female or MaleHomozygous for Mmp12tm1Sds  
Price per Pair (US dollars $)Pair Genotype
$519.80Homozygous for Mmp12tm1Sds x Homozygous for Mmp12tm1Sds  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(6.8)