Strain Name: |
B6;129S1-Tshrtm1Rmar/J |
|---|---|
Stock Number: |
004858 |
Availability: | Repository-Cryopreserved |
Price and Supply Information | |
General Terms and Conditions |
| Genes & Alleles | Tshr; Tshrtm1Rmar; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse Donating Investigator Terry Davies, Mount Sinai School of Medicine Strain Description
Mice that are homozygous for the targeted mutation exhibit delayed eye opening, are runted by day 21, and will die within 1 week if weaned at day 21. Homozygotes will survive by extending weaning to 28 days but will not reproduce. Homozygotes are viable and fertile when weaned at day 21 and subsequently placed on a hormone replacement therapy diet consisting of a 100ppm desiccated thyroid powder supplement. No gene product (mRNA or protein) is detected by RT-PCR or Western blot analysis of thyroid membranes. Enhanced Green Fluorescent Protein (EGFP) gene product is detected by RT-PCR and Western blot analysis of thyroid tissue. Hypothyroidism is exhibited by mutant mice that have a longer weaning period and a non-supplemented diet, as indicated by low T4 and T3 serum levels, and high thyrotropin (TSH) serum levels. Treatment with exogenous TSH does not result in a thyroid hormone release response. Mutant mice produce uniodinated thyroglobulin and do not accumulate radioactive iodide in their thyroid glands, although in vitro cultured thyroid slices have restored iodide uptake ability when cultured in the presence of forskolin (an adenylate cyclase agonist). Sodium-iodide symporter (NIS) protein is undetectable in thyroid tissue by Western blot analysis. Histological examination of mutant thyroid tissue reveals reduced size (approximately 50% of wildtype) and number of follicles. EGFP is detected by fluorescence microscopy in thyroid glands of both homozygotes and heterozygotes. This mutant mouse strain may be useful in studies of hypothyroidism.Strain Development
A targeting vector containing an Enhanced Green Fluorescent Protein-neomycin resistance cassette (EGFP-neo, Clonetech) and herpes simplex virus thymidine kinase gene was used to disrupt exon 1 of the targeted gene. The construct was electroporated into 129S1/Sv-p+ Tyr+ KitlSl-J/+ derived W9.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting male chimeric animals were crossed to C57BL/6J mice. Heterozygotes were intercrossed to generate homozygotes.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tshrtm1Rmar | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Russell Marians | ||
| Common Name(s) | TSHR-KO; | ||
| Mutation Made By | Russell Marians, | ||
| Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> | ||
| ES Cell Line Name | W9.5/W95 | ||
| ES Cell Line Strain | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> | ||
| Site of Expression | Thyroid tissue | ||
| Gene Symbol and Name | Tshr, thyroid stimulating hormone receptor | ||
| Chromosome | 12 | ||
| Gene Common Name(s) | AI481368; LGR3; MGC75129; TSHRA; expressed sequence AI481368; hTSHR-I; hypothroid; hypothyroid; hyt; pet; petite; | ||
| Molecular Note | The endogenous locus was disrupted by an EGFP-neo cassette inserted by homologous recombination. The absence of normal transcript and protein in homozygous mutant mice was demonstrated by RT-PCR and Western blot analyses. GFP expression was confirmed at both the transcript and protein levels. [MGI Ref ID J:80513] | ||
Control Information
| Control | ||
|---|---|---|
| 101043 B6129SF1/J | (approximate) | |
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Genotyping Protocols
Tshrtm1Rmar
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice can be bred as heterozygotes or homozygotes. If weaned at 21 d, homozygous mice die within 1 week unless placed on a hormone replacement therapy diet consisting of 100 ppm dessicated thyroid powder (see Marians PNAS 2002 99:15776-81). Further, homozygotes will survive by extending weaning to 28 days but will not reproduce. |
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Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (139 strains)
000805 CBy.RF-Tshrhyt/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Research Applications
This mouse can be used to support research in many areas including:
Developmental Biology Research
Skeletal Defects
Endocrine Deficiency Research
Adipose Defects
Thyroid Defects
Internal/Organ Research
Adipose Defects
Thyroid Defects
Metabolism Research
Mouse/Human Gene Homologs
hypothyroidism
Research Tools
Fluorescent Proteins
Metabolism Research
References
Selected Reference(s)
Additional ReferencesMarians RC; Ng L; Blair HC; Unger P; Graves PN; Davies TF. 2002. Defining thyrotropin-dependent and -independent steps of thyroid hormone synthesis by using thyrotropin receptor-null mice. Proc Natl Acad Sci U S A 99(24):15776-81. [PubMed: 12432094] [MGI Ref ID J:80513]
Price and Supply Information
| Strain Name: | B6;129S1-Tshrtm1Rmar/J |
| Stock Number: | 004858 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
USA, Canada and Mexico
| Price(s) in US dollars ($) | |||||
|---|---|---|---|---|---|
| Cryorecovery Fee | $1900.00 | ||||
| Cryopreserved Embryos Fee | $1600.00 | ||||
Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to the Supply Notes for further information. |
|---|---|
| Supply Notes |
Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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