Strain Name:

CByJ.B6-Inpp4awbl/FrkJ

Stock Number:

004913

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Availability:

Cryopreserved - Ready for recovery

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Spontaneous Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Background Strain BALB/cByJ
Donor Strain C57BL/6J-Aw

Appearance
albino
Related Genotype: A/A Tyrp1b/Tyrp1b Tyrc/Tyrc

Description
Mice homozygous for Inpp4awbl exhibit small size, ataxia, and cell death in the cerebellum, hippocampus (CA1) and neocortex. Homozygotes die between two and five weeks probably from seizures and/or failure to thrive. Heterozgotes do not display an overt locomotor defect.

Development
The Inpp4awbl mutation arose on C57BL/6J-Aw in 1996 at The Jackson Laboratory. It was backcrossed to BALB/cByJ for ten generations in the laboratory of Dr. Wayne Frankel (Frk). Nystuen et al. describes the mutant phenotype as observed on the original background. According to a personal communication from the donating investigator, Dr. Frankel, the phenotype does not differ on this background. This strain was donated to The Jackson Laboratory in 2004.

Control Information

  Control
   Wild-type from the colony
   001026 BALB/cByJ
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Inpp4awbl/Inpp4awbl

        C57BL/6J-Aw
  • mortality/aging
  • complete postnatal lethality
    • mice usually die by P24; posture suggests that mice die from maximal seizures, although a general failure to thrive cannot be excluded   (MGI Ref ID J:72427)
  • behavior/neurological phenotype
  • ataxia
    • severe, related to cerebellar defects; detectable at P14   (MGI Ref ID J:72427)
  • impaired righting response   (MGI Ref ID J:72427)
  • growth/size/body phenotype
  • decreased body size   (MGI Ref ID J:72427)
  • nervous system phenotype
  • Purkinje cell degeneration
    • detected as early as P6, widespread by P8, and severe by P12   (MGI Ref ID J:72427)
  • hippocampal neuron degeneration
    • pronounced pyramidal cell loss detected at P21   (MGI Ref ID J:72427)
  • small cerebellum
    • detected as early as P8 with further degeneration noted at P21   (MGI Ref ID J:72427)
  • thin external granule cell layer   (MGI Ref ID J:72427)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Inpp4awbl related

Neurobiology Research
Ataxia (Movement) Defects
Cerebellar Defects
Epilepsy

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Inpp4awbl
Allele Name weeble
Allele Type Spontaneous
Strain of OriginC57BL/6J-A
Gene Symbol and Name Inpp4a, inositol polyphosphate-4-phosphatase, type I
Chromosome 1
Gene Common Name(s) 107kDa; INPP4; R74740; TVAS1; expressed sequence R74740;
Molecular Note The underlying mutation underlying this allele was determined to be a single nucleotide deletion (G at nucleotide position 744). The resulting frameshift creates a stop codon at amino acid position 263, truncating most of the 939 amino acid protein including the active site. [MGI Ref ID J:72427]

Genotyping

Genotyping Information

Genotyping Protocols

Inpp4awbl, End Point Analysis


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Nystuen A; Legare ME; Shultz LD; Frankel WN. 2001. A null mutation in inositol polyphosphate 4-phosphatase type I causes selective neuronal loss in weeble mutant mice. Neuron 32(2):203-12. [PubMed: 11683991]  [MGI Ref ID J:72427]

Additional References

Inpp4awbl related

Ivetac I; Gurung R; Hakim S; Horan KA; Sheffield DA; Binge LC; Majerus PW; Tiganis T; Mitchell CA. 2009. Regulation of PI(3)K/Akt signalling and cellular transformation by inositol polyphosphate 4-phosphatase-1. EMBO Rep 10(5):487-93. [PubMed: 19325558]  [MGI Ref ID J:157366]

Ivetac I; Munday AD; Kisseleva MV; Zhang XM; Luff S; Tiganis T; Whisstock JC; Rowe T; Majerus PW; Mitchell CA. 2005. The type Ialpha inositol polyphosphate 4-phosphatase generates and terminates phosphoinositide 3-kinase signals on endosomes and the plasma membrane. Mol Biol Cell 16(5):2218-33. [PubMed: 15716355]  [MGI Ref ID J:100215]

Sachs AJ; David SA; Haider NB; Nystuen AM. 2009. Patterned neuroprotection in the Inpp4a(wbl) mutant mouse cerebellum correlates with the expression of Eaat4. PLoS One 4(12):e8270. [PubMed: 20011524]  [MGI Ref ID J:155961]

Sasaki J; Kofuji S; Itoh R; Momiyama T; Takayama K; Murakami H; Chida S; Tsuya Y; Takasuga S; Eguchi S; Asanuma K; Horie Y; Miura K; Davies EM; Mitchell C; Yamazaki M; Hirai H; Takenawa T; Suzuki A; Sasaki T. 2010. The PtdIns(3,4)P(2) phosphatase INPP4A is a suppressor of excitotoxic neuronal death. Nature 465(7297):497-501. [PubMed: 20463662]  [MGI Ref ID J:161963]

Shin HW; Hayashi M; Christoforidis S; Lacas-Gervais S; Hoepfner S; Wenk MR; Modregger J; Uttenweiler-Joseph S; Wilm M; Nystuen A; Frankel WN; Solimena M; De Camilli P; Zerial M. 2005. An enzymatic cascade of Rab5 effectors regulates phosphoinositide turnover in the endocytic pathway. J Cell Biol 170(4):607-18. [PubMed: 16103228]  [MGI Ref ID J:101018]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

General Supply Notes

  • View the complete collection of spontaneous mutants in the Mouse Mutant Resource.

Control Information

  Control
   Wild-type from the colony
   001026 BALB/cByJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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