Description

Strain Information

Former Names NOD.B6-Itgb7tm1Cgn/2Lt    (Changed: 12-JUL-05 ) Type Congenic; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Background Strain NOD/ShiLt Donor Strain C57BL/6 H2 Haplotype g7 Generation N10F7p (01-MAY-05)   Donating Investigator Edward Leiter,   The Jackson Laboratory

Appearance
albino
Related Genotype: A/A Tyr^c/Tyr^c

Description
Mice homozygous for the targeted mutation have a marked hypoplasia of the gut-associated lymphoid tissue(GALT). This deficit is apparently due to the failure of Itgb7 deficient lymphocytes to arrest and adhere in the GALT.

Development
This strain was derived from an initial cross between mice of strains NOD/Lt and C57BL/6-Itgb7^tm1Cgn (Stock No. 002965), followed by 10 backcrosses (to N11) to NOD/Lt of progeny selected for heterozygosity for the targeted allele. Heterozygous offspring of the final backcross were intercrossed to initiate a line homozygous for Itgb7^tm1Cgn, and the strain was then maintained by sibling inbreeding. Statistically, this strain's genome is approximately 99.95% of NOD/Lt origin. Marker assisted analysis of all known Idd loci (1-21) are of NOD origin. This stock was transferred from Ed Leiter's research colony into the T1DR via rederivation.

Control Information

	Control
	001976 NOD/ShiLtJ
Considerations for Choosing Controls

Related Strains

Strains carrying   Itgb7^tm1Cgn allele

002965	C57BL/6-Itgb7tm1Cgn/J
004943	NOD.Cg-Selltm1Tft Itgb7tm1Cgn/LtJ

View Strains carrying   Itgb7^tm1Cgn     (2 strains)

Strains carrying other alleles of Itgb7

007707

C57BL/6-Itgb7tm1Mshi/J

View Strains carrying other alleles of Itgb7     (1 strain)

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Itgb7^tm1Cgn related

Cell Biology Research
Defects in Cell Adhesion Molecules

Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines
Lymphoid Tissue Defects

Currently there is no phenotype information for this strain.

Genes & Alleles

Gene & Allele Information

Allele Symbol		Itgb7tm1Cgn
Allele Name		targeted mutation 1, University of Cologne
Allele Type		Targeted (knock-out)
Common Name(s)		beta7-;
Mutation Made By		Norbert Wagner,   University of Cologne
Strain of Origin		C57BL/6
ES Cell Line Name		BL/6-III
ES Cell Line Strain		C57BL/6
Gene Symbol and Name		Itgb7, integrin beta 7
Chromosome		15
Molecular Note		A neomycin resistance cassette replaced part of exon 12 and all of exons 13 and 14, which encode the transmembrane and part of the extracellular domains. [MGI Ref ID J:34306]

Genotyping

Genotyping Information

Genotyping Protocols

Itgb7^tm1Cgn, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Wagner N; Lohler J; Kunkel EJ; Ley K; Leung E; Krissansen G; Rajewsky K; Muller W. 1996. Critical role for beta7 integrins in formation of the gut-associated lymphoid tissue. Nature 382(6589):366-70. [PubMed: 8684468]  [MGI Ref ID J:34306]

Additional References

Itgb7^tm1Cgn related

Abonia JP; Hallgren J; Jones T; Shi T; Xu Y; Koni P; Flavell RA; Boyce JA; Austen KF; Gurish MF. 2006. Alpha-4 integrins and VCAM-1, but not MAdCAM-1, are essential for recruitment of mast cell progenitors to the inflamed lung. Blood 108(5):1588-94. [PubMed: 16670268]  [MGI Ref ID J:138072]

Apostolaki M; Manoloukos M; Roulis M; Wurbel MA; Muller W; Papadakis KA; Kontoyiannis DL; Malissen B; Kollias G. 2008. Role of beta7 integrin and the chemokine/chemokine receptor pair CCL25/CCR9 in modeled TNF-dependent Crohn's disease. Gastroenterology 134(7):2025-35. [PubMed: 18439426]  [MGI Ref ID J:136667]

Artis D; Humphreys NE; Potten CS; Wagner N; Muller W; McDermott JR; Grencis RK; Else KJ. 2000. Beta7 integrin-deficient mice: delayed leukocyte recruitment and attenuated protective immunity in the small intestine during enteric helminth infection Eur J Immunol 30(6):1656-64. [PubMed: 10898502]  [MGI Ref ID J:62733]

Berberich S; Dahne S; Schippers A; Peters T; Muller W; Kremmer E; Forster R; Pabst O. 2008. Differential molecular and anatomical basis for B cell migration into the peritoneal cavity and omental milky spots. J Immunol 180(4):2196-203. [PubMed: 18250426]  [MGI Ref ID J:132002]

Bry L; Brenner MB. 2004. Critical role of T cell-dependent serum antibody, but not the gut-associated lymphoid tissue, for surviving acute mucosal infection with Citrobacter rodentium, an attaching and effacing pathogen. J Immunol 172(1):433-41. [PubMed: 14688352]  [MGI Ref ID J:87077]

Croy BA; Ashkar AA; Foster RA; DiSanto JP; Magram J; Carson D; Gendler SJ; Grusby MJ; Wagner N; Muller W; Guimond MJ. 1997. Histological studies of gene-ablated mice support important functional roles for natural killer cells in the uterus during pregnancy J Reprod Immunol 35(2):111-33. [PubMed: 9421796]  [MGI Ref ID J:44813]

Czarneski J; Berguer P; Bekinschtein P; Kim DC; Hakimpour P; Wagner N; Nepomnaschy I; Piazzon I; Ross SR. 2002. Neonatal infection with a milk-borne virus is independent of beta7 integrin- and L-selectin-expressing lymphocytes. Eur J Immunol 32(4):945-56. [PubMed: 11920560]  [MGI Ref ID J:76001]

Dutt S; Ermann J; Tseng D; Liu YP; George TI; Fathman CG; Strober S. 2005. L-selectin and beta7 integrin on donor CD4 T cells are required for the early migration to host mesenteric lymph nodes and acute colitis of graft-versus-host disease. Blood 106(12):4009-15. [PubMed: 16105972]  [MGI Ref ID J:124067]

Fernekorn U; Butcher EC; Behrends J; Hartz S; Kruse A. 2004. Functional involvement of P-selectin and MAdCAM-1 in the recruitment of alpha4beta7-integrin-expressing monocyte-like cells to the pregnant mouse uterus. Eur J Immunol 34(12):3423-33. [PubMed: 15484189]  [MGI Ref ID J:94597]

Forbes E; Hulett M; Ahrens R; Wagner N; Smart V; Matthaei KI; Brandt EB; Dent LA; Rothenberg ME; Tang M; Foster PS; Hogan SP. 2006. ICAM-1-dependent pathways regulate colonic eosinophilic inflammation. J Leukoc Biol 80(2):330-41. [PubMed: 16731772]  [MGI Ref ID J:111227]

Ghosh S; Chackerian AA; Parker CM; Ballantyne CM; Behar SM. 2006. The LFA-1 adhesion molecule is required for protective immunity during pulmonary Mycobacterium tuberculosis infection. J Immunol 176(8):4914-22. [PubMed: 16585587]  [MGI Ref ID J:131154]

Gurish MF; Tao H; Abonia JP; Arya A; Friend DS; Parker CM; Austen KF. 2001. Intestinal mast cell progenitors require CD49dbeta7 (alpha4beta7 integrin) for tissue-specific homing. J Exp Med 194(9):1243-52. [PubMed: 11696590]  [MGI Ref ID J:119138]

Ho J; Kurtz CC; Naganuma M; Ernst PB; Cominelli F; Rivera-Nieves J. 2008. A CD8+/CD103high T cell subset regulates TNF-mediated chronic murine ileitis. J Immunol 180(4):2573-80. [PubMed: 18250468]  [MGI Ref ID J:131978]

Kuklin NA; Rott L; Feng N; Conner ME; Wagner N; Muller W; Greenberg HB. 2001. Protective intestinal anti-rotavirus B cell immunity is dependent on alpha(4)beta(7) integrin expression but does not require IgA antibody production. J Immunol 166(3):1894-902. [PubMed: 11160237]  [MGI Ref ID J:67098]

Kunkel EJ; Ramos CL; Steeber DA; Muller W; Wagner N; Tedder TF; Ley K. 1998. The roles of L-selectin, beta 7 integrins, and P-selectin in leukocyte rolling and adhesion in high endothelial venules of Peyer's patches. J Immunol 161(5):2449-56. [PubMed: 9725243]  [MGI Ref ID J:112049]

Lammermann T; Bader BL; Monkley SJ; Worbs T; Wedlich-Soldner R; Hirsch K; Keller M; Forster R; Critchley DR; Fassler R; Sixt M. 2008. Rapid leukocyte migration by integrin-independent flowing and squeezing. Nature 453(7191):51-5. [PubMed: 18451854]  [MGI Ref ID J:134784]

Mancassola R; Lacroix-Lamande S; Barrier M; Naciri M; Salmon H; Laurent F. 2004. Increased susceptibility of beta7-integrin-deficient neonatal mice in the early stage of Cryptosporidium parvum infection. Infect Immun 72(6):3634-7. [PubMed: 15155674]  [MGI Ref ID J:90262]

Park EJ; Mora JR; Carman CV; Chen J; Sasaki Y; Cheng G; von Andrian UH; Shimaoka M. 2007. Aberrant activation of integrin alpha4beta7 suppresses lymphocyte migration to the gut. J Clin Invest 117(9):2526-38. [PubMed: 17786243]  [MGI Ref ID J:127413]

Steeber DA; Tang ML; Zhang XQ; Muller W; Wagner N; Tedder TF. 1998. Efficient lymphocyte migration across high endothelial venules of mouse Peyer's patches requires overlapping expression of L-selectin and beta7 integrin. J Immunol 161(12):6638-47. [PubMed: 9862692]  [MGI Ref ID J:112112]

Sydora BC; Wagner N; Lohler J; Yakoub G; Kronenberg M; Muller W; Aranda R. 2002. beta7 Integrin expression is not required for the localization of T cells to the intestine and colitis pathogenesis. Clin Exp Immunol 129(1):35-42. [PubMed: 12100020]  [MGI Ref ID J:115570]

Tu L; Poe JC; Kadono T; Venturi GM; Bullard DC; Tedder TF; Steeber DA. 2002. A functional role for circulating mouse L-selectin in regulating leukocyte/endothelial cell interactions in vivo. J Immunol 169(4):2034-43. [PubMed: 12165530]  [MGI Ref ID J:120206]

Wagner N; Lohler J; Tedder TF; Rajewsky K; Muller W; Steeber DA. 1998. L-selectin and beta7 integrin synergistically mediate lymphocyte migration to mesenteric lymph nodes. Eur J Immunol 28(11):3832-9. [PubMed: 9842926]  [MGI Ref ID J:114211]

Waldman E; Lu SX; Hubbard VM; Kochman AA; Eng JM; Terwey TH; Muriglan SJ; Kim TD; Heller G; Murphy GF; Liu C; Alpdogan O; van den Brink MR. 2006. Absence of beta7 integrin results in less graft-versus-host disease because of decreased homing of alloreactive T cells to intestine. Blood 107(4):1703-11. [PubMed: 16291587]  [MGI Ref ID J:129395]

Wang C; McDonough JS; McDonald KG; Huang C; Newberry RD. 2008. Alpha4beta7/MAdCAM-1 interactions play an essential role in transitioning cryptopatches into isolated lymphoid follicles and a nonessential role in cryptopatch formation. J Immunol 181(6):4052-61. [PubMed: 18768861]  [MGI Ref ID J:139096]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Type 1 Diabetes Repository collection.

Control Information

	Control
	001976 NOD/ShiLtJ
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

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In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.