Strain Name: |
B6;129P2-Omptm2(spH)Mom/J |
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Stock Number: |
004946 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Strain Common Names | OMP-spH mice; OMP-synapto-pHluorin mice; |
| Genes & Alleles | Omp; Omptm2(spH)Mom; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse Donating Investigator Peter Mombaerts, Max Planck Institute of Biophysics Generation F?+N1F1+N1p (07-AUG-05) Strain Description
These mutant mice express synapto-pHluorin (spH) from the endogenous locus encoding the olfactory marker protein (OMP) as a result of a targeted knockin mutation that replaces the OMP coding region with that of spH. The OMP locus directs expression of spH at high levels and exclusively in mature sensory neurons of the main olfactory epithelium (OSNs) and the vomeronasal epithelium. SpH is a pH-sensitive variant of the green fluorescent protein (ecliptic pHluorin) fused to the mouse synaptic vesicle-associated protein (VAMP2). The protein is localized preferentially in synaptic vesicles, but is also present on the plasma membrane of OSN axons and nerve terminals. The fluorescent domain of spH is exposed to the acidic lumen of synaptic vesicles where it is ~ 20 fold less fluorescent than at neutral pH. Upon synaptic release, the lumen of the synaptic vesicles becomes continuous with the extracellular space resulting in an increase in pH that causes a rapid increase in fluorescence. In mutant mice all glomeruli of the olfactory bulb are robustly labeled. There are ~1800 glomeruli in the main olfactory bulb, and each glomerulus receives axonal input from thousands of OSNs that express the same odorant receptor. Glomeruli in the dorsal surface of the olfactory bulb can be observed in vivo, after thinning the skull, through a standard epifluorescence microscope. Fluorescence changes caused by neuronal activity and presynaptic release can be measured in living mice following odorant stimulation. Mice that are homozygous for this allele do not express gene product (OMP) but are viable, fertile, normal in size and do not display gross physical or behavioral abnormalities. Homozygous mice produce small litters and breed at reduced frequency. This mutant mouse strain should prove useful to measure stimulus-induced activity or presynaptic vesicle release in OSNs of living mice.Strain Development
A targeting vector containing super-ecliptic synapto-pHluorin coding sequence and an auto-excising neomycin resistance cassette was used to replace the Omp coding region sequence in 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. The loxP-flanked auto-excising neomycin resistance cassette is composed of the mouse angiotensin-converting enzyme promoter (tACE) located 5' of sequences encoding Cre recombinase and the neomycin resistance gene. The angiotensin-converting enzyme promoter has been shown to initiate transcription only during spermatogenesis. Correctly targeted ES cells were injected into C57BL/6 blastocysts and a chimeric animal derived from cell clone OSE-80 was obtained.The plasmid that was used to generate this mutation is banked and described at Addgene.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Omptm2(spH)Mom | ||
|---|---|---|---|
| Allele Name | targeted muation 2, Peter Mombaerts | ||
| Common Name(s) | OMP-spH; OMP-synapto-pHluorin; | ||
| Mutation Made By | Peter Mombaerts, Max Planck Institute of Biophysics | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Site of Expression | active mature sensory neurons of the main olfactory epithelium (OSNs) and the vomeronasal epithelium; protein localized preferentially in synaptic vesicles, but is also present on the plasma membrane of OSN axons and nerve terminals; all glomeruli of the olfactory bulb are robustly labeled (visible in vivo); pH sensitive | ||
| Gene Symbol and Name | Omp, olfactory marker protein | ||
| Chromosome | 7 | ||
| General Note | In the acidic lumen of pre-synaptic vesicles, the fluorescence of spH is only about 5% of its intensity at neutral pH. When synaptic vesicles with spH anchored to their lumenal face fuse with the plasma membrane to release their contents, the spH is exposed to the pH-neutral extracellular environmment and rapidly becomes highly fluorescent. All olfactory bulb glomeruli fluoresce, with varying intensity. The superficial nerve layer overlying the glomerular layer and comprising axons of olfactory sensory neurons is more weakly fluorescent. In vivo fluorescent imaging of the olfactory bulb demonstrates that odorant-evoked responses cause strong fluorescence of odorant-specific, bilaterally symmetric groups of glomeruli. | ||
| Molecular Note | The coding exon was replaced by a construct containing the ecliptic synaptopHluorin (spH) coding sequence and a self-excising neomycin resistance cassette. SpH is a fusion protein joining a pH-sensitive derivative of wild-type green fluorescent protein (GFP) to the carboxyl terminus of a mouse synaptic vesicle-associated membrane protein (VAMP2), which targets spH to the lumenal face of pre-synaptic vesicles. The loxP-flanked, male-germline self-excising neomycin resistance cassette (which was deleted inthe chimeric founder's germline) contains the Cre recombinase coding sequence under control of the mouse testis-specific angiotensin-converting enzyme (tACE) promoter and tk-neo. The endogenous gene regulatory sequences direct spH/Vamp expression specifically to sensory neurons in glomeruli of the olfactory bulb. [MGI Ref ID J:36595] [MGI Ref ID J:79620] [MGI Ref ID J:88765] | ||
Control Information
| Control | ||
|---|---|---|
| 100903 B6129PF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
Genotyping Protocols
Omptm2(spH)Mom
Colony Maintenance
| Breeding & Husbandry | This strain originated on a B6;129P2 background. It is maintained as a homozygote on the same background. |
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| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Omp
007768 B6.129S2-Omptm1Mom/MomJ 006667 B6;129P2-Omptm3Mom/MomJ 006668 B6;129P2-Omptm4(cre)Mom/MomJ 006594 B6;129S2-Omptm1Mom/MomJ View Strains carrying other alleles of Omp (4 strains)
Research Applications
This mouse can be used to support research in many areas including:
Neurobiology Research
Fluorescent protein expression in neural tissue
Research Tools
Genetics Research (Tissue/Cell Markers: neurons)
Neurobiology Research (cell marker)
Sensorineural Research
References
Selected Reference(s)
Additional ReferencesBozza T; McGann JP; Mombaerts P; Wachowiak M. 2004. In vivo imaging of neuronal activity by targeted expression of a genetically encoded probe in the mouse. Neuron 42(1):9-21. [PubMed: 15066261] [MGI Ref ID J:88765]
Price and Supply Information
| Strain Name: | B6;129P2-Omptm2(spH)Mom/J |
| Stock Number: | 004946 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
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Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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