Description

Strain Information

Former Names NOD.129S2(B6)-Casp1tm1Sesh/LtJ    (Changed: 24-APR-13 ) Type Deletion; Additional information on Mice with Chromosomal Aberrations. Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote         (Female x Male)   01-MAR-06 Species laboratory mouse Background Strain NOD/ShiLt Donor Strain 129S2 Generation [N10F13p]+F16N1F6 (29-JUN-11) Generation Definitions   Donating Investigator Dr. Edward Leiter,   The Jackson Laboratory

Appearance
albino, pink-eyed
Related Genotype: A/? Tyr^c/Tyr^c

Description
Casp1^tm1Sesh homozygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. There is no detectable expression of Casp1 in spleen by northern blot analysis or by RT-PCR of peritoneal exudate cells, brain, lung, heart, liver, adrenal gland, kidney, testis, and thymus (Li et al, 1995). Cultured LPS stimulated bone marrow derived macrophages from homozygous NOD.129S2(B6)- Casp1^tm1Sesh /LtJ animals secrete 4-fold less IL1 beta, 30% less IL1 alpha, and IL18 is undetectable when compared with hemizygous and wild-type controls. Diabetes frequency of Casp1^tm1Sesh deficient animals is equivalent to NOD/Lt, heterozygote and wild-type controls. Weanling Casp1^tm1Sesh homozygous animals injected with Complete Freund's adjuvant and young pre-diabetic males treated with multiple low dose streptozotocin behave similarly to control (wild type, heterozygote, or NOD/Lt) animals (Schott et al, 2004). NOD.129S2(B6)- Casp1^tm1Sesh/LtJ is a useful model for studying the role of IL1 and IL18 cytokines in inflammatory processes relating to diabetes.

Development
A construct containing a neomycin expression cassette inserted into exon 6 of Casp1 (cloned from a 129/Sv mouse), deleting 31 bp of sequence encoding the region of the catalytic active site and rendering the sequence out of frame after the insertion, was transfected into D3 (129S2/SvPas derived) embryonic stem cells (ES cells). These ES cells were injected into C57BL/6 blastocysts. Chimeric founders were initially mated to C57BL/6 and subsequently intercrossed to generate homozygotes (Li et al, 1995). In 1998, Dr. Edward Leiter at The Jackson Laboratory received B6.129S2-Casp1^tm1Sesh mice from Dr. Winnie Wong, BASF Bioresearch Corporation and backcrossed this mutation to NOD/Lt for 10 generations, subsequently intercrossing to generate homozygotes (Schott et al. 2004). In 2004, NOD.129S2(B6)- Casp1^tm1Sesh /LtJ homozygous strain at N10F11 was transferred from Edward Leiter's research colony to a Jackson Laboratory distribution colony.

Control Information

	Control
	001976 NOD/ShiLtJ
Considerations for Choosing Controls

Related Strains

Strains carrying   Casp1^tm1Sesh allele

005346

NOD.Cg-Il10tm1Cgn Casp1tm1Sesh Casp4del/LtJ

View Strains carrying   Casp1^tm1Sesh     (1 strain)

Strains carrying   Casp4^del allele

005346

NOD.Cg-Il10tm1Cgn Casp1tm1Sesh Casp4del/LtJ

View Strains carrying   Casp4^del     (1 strain)

Strains carrying other alleles of Casp1

016621

B6N.129S2-Casp1tm1Flv/J

View Strains carrying other alleles of Casp1     (1 strain)

Phenotype

Phenotype Information

View Phenotypic Data

Phenotypic Data

Mouse Phenome Database

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI

- Model with phenotypic similarity to human disease where etiologies are distinct. Human genes are associated with this disease. Orthologs of these genes do not appear in the mouse genotype(s).

Diabetes Mellitus, Insulin-Dependent; IDDM

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Casp1^tm1Sesh/Casp1^tm1Sesh Casp4^del/Casp4^del

        NOD.129S2(B6)-Casp1^tm1Sesh Casp4^del/LtJ

• immune system phenotype
• decreased interleukin-1 alpha secretion
  • cultured LPS-stimulated bone marrow derived macrophages from homozygous mutants secrete 20%-30% less IL-1alpha relative to heterozygous or wild-type (NOD/Lt) mice   (MGI Ref ID J:87250)
• decreased interleukin-1 beta secretion
  • cultured LPS-stimulated bone marrow derived macrophages from homozygous mutants secrete 4-fold less IL-1beta relative to heterozygous or wild-type (NOD/Lt) mice   (MGI Ref ID J:87250)
• decreased interleukin-18 secretion
  • cultured LPS-stimulated bone marrow derived macrophages from homozygotes produce no immunoreactive IL18 relative to heterozygous or wild-type (NOD/Lt) mice   (MGI Ref ID J:87250)
• homeostasis/metabolism phenotype
• *normal* homeostasis/metabolism phenotype
  • homozygotes show no significant differences in the rate or in total incidence of diabetes relative to heterozygotes or wild-type (NOD/Lt) control mice   (MGI Ref ID J:87250)
  • weanling homozygotes injected with Complete Freund's adjuvant and young pre-diabetic males treated with multiple low dose streptozotocin behave similarly to control (wild-type, heterozygous, or NOD/Lt) mice   (MGI Ref ID J:87250)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Casp1^tm1Sesh related

Diabetes and Obesity Research
Type 1 Diabetes (IDDM)

Immunology, Inflammation and Autoimmunity Research
Immunodeficiency
      B cell defects

Research Tools
Diabetes and Obesity Research

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Casp1tm1Sesh
Allele Name		targeted mutation 1, Tara Seshadri
Allele Type		Targeted (knock-out)
Common Name(s)		Casp1 -; Casp1-/Casp11129mt; Caspase-1-; ICE -; casp-1-;
Mutation Made By		Dr. Edward Leiter,   The Jackson Laboratory
Strain of Origin		129S2/SvPas
ES Cell Line Name		D3
ES Cell Line Strain		129S2/SvPas
Gene Symbol and Name		Casp1, caspase 1
Chromosome		9
Gene Common Name(s)		Caspase-1; ICE; IL1BC; Il1bc; P45; interleukin 1 beta convertase; interleukin 1 beta-converting enzyme;
General Note		The ES cells used to generate this allele contain the linked Casp4del truncated allele that fails to produce a functional Casp4. Phenotypes associated with this allele may be affected by the presence of the Caspdel allele. J:193522
Molecular Note		A neomycin expression cassette was inserted into exon 6, deleting 31 bp of sequence encoding the region of the active site and rendering the sequence out of frame after the insertion. Northern blot analysis on spleen RNA demonstrated an absence of the normal transcript in homozygous mice, and western blot analysis showed that the protein was not expressed in peritoneal macrophages of homozygous mice. This allele was generated in ES cells that lack protein expression of Casp11 (Casp129mt). [MGI Ref ID J:22964]
Allele Symbol		Casp4del
Allele Name		deletion
Allele Type		Spontaneous
Common Name(s)		Casp129;
Strain of Origin		129P3/J and 129S1/SvImJ and 129S2/SvPas and 129S6/SvEvTac and 129X1/SvJ
Gene Symbol and Name		Casp4, caspase 4, apoptosis-related cysteine peptidase
Chromosome		9
Gene Common Name(s)		Casp11; Caspase-11; ICE(rel)II; ICEREL-II; ICH-2; Mih1/TX; TX; capase 11, apoptosis-related cysteine protease; caspase 11, apoptosis-related cysteine peptidase; ich-3;
Molecular Note		RT-PCR confirmed that five 129 substrains (129X1/SvJ, 129S1/SvImJ, 129S2/SvPas, 129S6/SvEvTac and 129P3/J) express a transcript that lacks exon 7 (delta110 isoform). Sequencing identified a 5 bp deletion in exon 7 that results in the fusion of exon 6 and8, a frame-shift after proline 304 and a stop codon after 5 aberrant amino acids. This deletion is not present in C57BL/6. Western blot analysis confirmed the absence of protein expression in LPS-primed macrophage. [MGI Ref ID J:193522]

Genotyping

Genotyping Information

Genotyping Protocols

Casp1^tm1Sesh, Fast MCA
Casp1^tm1Sesh, Separated PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Schott WH; Haskell BD; Tse HM; Milton MJ; Piganelli JD; Choisy-Rossi CM; Reifsnyder PC; Chervonsky AV; Leiter EH. 2004. Caspase-1 Is Not Required for Type 1 Diabetes in the NOD Mouse. Diabetes 53(1):99-104. [PubMed: 14693703]  [MGI Ref ID J:87250]

Additional References

Cumberbatch M; Dearman RJ; Antonopoulos C; Groves RW; Kimber I. 2001. Interleukin (IL)-18 induces Langerhans cell migration by a tumour necrosis factor-alpha- and IL-1beta-dependent mechanism. Immunology 102(3):323-30. [PubMed: 11298831]  [MGI Ref ID J:68644]

Jolicoeur P; Hu C; Mak TW; Martinou JC; Kay DG. 2003. Protection against murine leukemia virus-induced spongiform myeloencephalopathy in mice overexpressing Bcl-2 but not in mice deficient for interleukin-6, inducible nitric oxide synthetase, ICE, Fas, Fas ligand, or TNF-R1 genes. J Virol 77(24):13161-70. [PubMed: 14645573]  [MGI Ref ID J:86761]

Li P; Allen H; Banerjee S; Franklin S; Herzog L; Johnston C; McDowell J; Paskind M; Rodman L; Salfeld J; Towne E; Tracey D; Wardwell S; Wei FY; Wong W; Kamen R; Seshadri T. 1995. Mice deficient in IL-1 beta-converting enzyme are defective in production of mature IL-1 beta and resistant to endotoxic shock. Cell 80(3):401-11. [PubMed: 7859282]  [MGI Ref ID J:22964]

Li P; Allen H; Banerjee S; Seshadri T. 1997. Characterization of mice deficient in interleukin-1 beta converting enzyme. J Cell Biochem 64(1):27-32. [PubMed: 9015751]  [MGI Ref ID J:40691]

Casp1^tm1Sesh related

Aachoui Y; Leaf IA; Hagar JA; Fontana MF; Campos CG; Zak DE; Tan MH; Cotter PA; Vance RE; Aderem A; Miao EA. 2013. Caspase-11 protects against bacteria that escape the vacuole. Science 339(6122):975-8. [PubMed: 23348507]  [MGI Ref ID J:193387]

Anthony DA; Andrews DM; Chow M; Watt SV; House C; Akira S; Bird PI; Trapani JA; Smyth MJ. 2010. A role for granzyme M in TLR4-driven inflammation and endotoxicosis. J Immunol 185(3):1794-803. [PubMed: 20585036]  [MGI Ref ID J:162467]

Antonopoulos C; Cumberbatch M; Dearman RJ; Daniel RJ; Kimber I; Groves RW. 2001. Functional caspase-1 is required for Langerhans cell migration and optimal contact sensitization in mice. J Immunol 166(6):3672-7. [PubMed: 11238606]  [MGI Ref ID J:124939]

Antonopoulos C; Cumberbatch M; Mee JB; Dearman RJ; Wei XQ; Liew FY; Kimber I; Groves RW. 2008. IL-18 is a key proximal mediator of contact hypersensitivity and allergen-induced Langerhans cell migration in murine epidermis. J Leukoc Biol 83(2):361-7. [PubMed: 17984289]  [MGI Ref ID J:145093]

Boyden ED; Dietrich WF. 2006. Nalp1b controls mouse macrophage susceptibility to anthrax lethal toxin Nat Genet 38(2):240-244. [PubMed: 16429160]  [MGI Ref ID J:99748]

Chamberlain J; Evans D; King A; Dewberry R; Dower S; Crossman D; Francis S. 2006. Interleukin-1beta and signaling of interleukin-1 in vascular wall and circulating cells modulates the extent of neointima formation in mice. Am J Pathol 168(4):1396-403. [PubMed: 16565512]  [MGI Ref ID J:107324]

Cheng W; Shivshankar P; Li Z; Chen L; Yeh IT; Zhong G. 2008. Caspase-1 contributes to Chlamydia trachomatis-induced upper urogenital tract inflammatory pathologies without affecting the course of infection. Infect Immun 76(2):515-22. [PubMed: 18025098]  [MGI Ref ID J:130235]

Coers J; Vance RE; Fontana MF; Dietrich WF. 2007. Restriction of Legionella pneumophila growth in macrophages requires the concerted action of cytokine and Naip5/Ipaf signalling pathways. Cell Microbiol 9(10):2344-57. [PubMed: 17506816]  [MGI Ref ID J:148669]

Cumberbatch M; Dearman RJ; Antonopoulos C; Groves RW; Kimber I. 2001. Interleukin (IL)-18 induces Langerhans cell migration by a tumour necrosis factor-alpha- and IL-1beta-dependent mechanism. Immunology 102(3):323-30. [PubMed: 11298831]  [MGI Ref ID J:68644]

Dewamitta SR; Nomura T; Kawamura I; Hara H; Tsuchiya K; Kurenuma T; Shen Y; Daim S; Yamamoto T; Qu H; Sakai S; Xu Y; Mitsuyama M. 2010. Listeriolysin O-dependent bacterial entry into the cytoplasm is required for calpain activation and interleukin-1 alpha secretion in macrophages infected with Listeria monocytogenes. Infect Immun 78(5):1884-94. [PubMed: 20194588]  [MGI Ref ID J:160087]

Dorhoi A; Nouailles G; Jorg S; Hagens K; Heinemann E; Pradl L; Oberbeck-Muller D; Duque-Correa MA; Reece ST; Ruland J; Brosch R; Tschopp J; Gross O; Kaufmann SH. 2012. Activation of the NLRP3 inflammasome by Mycobacterium tuberculosis is uncoupled from susceptibility to active tuberculosis. Eur J Immunol 42(2):374-84. [PubMed: 22101787]  [MGI Ref ID J:179821]

Edelson BT; Unanue ER. 2002. MyD88-dependent but Toll-like receptor 2-independent innate immunity to Listeria: no role for either in macrophage listericidal activity. J Immunol 169(7):3869-75. [PubMed: 12244184]  [MGI Ref ID J:120406]

Feliciani C; Toto P; Amerio P; Pour SM; Coscione G; Shivji G; Wang B; Sauder DN. 2000. In vitro and in vivo expression of interleukin-1alpha and tumor necrosis factor-alpha mRNA in pemphigus vulgaris: interleukin-1alpha and tumor necrosis factor-alpha are involved in acantholysis. J Invest Dermatol 114(1):71-7. [PubMed: 10620118]  [MGI Ref ID J:127060]

Freigang S; Ampenberger F; Spohn G; Heer S; Shamshiev AT; Kisielow J; Hersberger M; Yamamoto M; Bachmann MF; Kopf M. 2011. Nrf2 is essential for cholesterol crystal-induced inflammasome activation and exacerbation of atherosclerosis. Eur J Immunol 41(7):2040-51. [PubMed: 21484785]  [MGI Ref ID J:177309]

Gehrig A; Janssen A; Horling F; Grimm C; Weber BH. 2006. The role of caspases in photoreceptor cell death of the retinoschisin-deficient mouse. Cytogenet Genome Res 115(1):35-44. [PubMed: 16974082]  [MGI Ref ID J:112879]

Gross O; Poeck H; Bscheider M; Dostert C; Hannesschlager N; Endres S; Hartmann G; Tardivel A; Schweighoffer E; Tybulewicz V; Mocsai A; Tschopp J; Ruland J. 2009. Syk kinase signalling couples to the Nlrp3 inflammasome for anti-fungal host defence. Nature 459(7245):433-6. [PubMed: 19339971]  [MGI Ref ID J:148538]

Hanley PJ; Kronlage M; Kirschning C; del Rey A; Di Virgilio F; Leipziger J; Chessell IP; Sargin S; Filippov MA; Lindemann O; Mohr S; Konigs V; Schillers H; Bahler M; Schwab A. 2012. Transient P2X7 receptor activation triggers macrophage death independent of Toll-like receptors 2 and 4, caspase-1, and pannexin-1 proteins. J Biol Chem 287(13):10650-63. [PubMed: 22235111]  [MGI Ref ID J:183298]

Henry T; Brotcke A; Weiss DS; Thompson LJ; Monack DM. 2007. Type I interferon signaling is required for activation of the inflammasome during Francisella infection. J Exp Med 204(5):987-94. [PubMed: 17452523]  [MGI Ref ID J:125737]

Jamilloux Y; Pierini R; Querenet M; Juruj C; Fauchais AL; Jauberteau MO; Jarraud S; Lina G; Etienne J; Roy CR; Henry T; Davoust N; Ader F. 2013. Inflammasome activation restricts Legionella pneumophila replication in primary microglial cells through flagellin detection. Glia 61(4):539-49. [PubMed: 23355222]  [MGI Ref ID J:193477]

Jayaraman P; Sada-Ovalle I; Beladi S; Anderson AC; Dardalhon V; Hotta C; Kuchroo VK; Behar SM. 2010. Tim3 binding to galectin-9 stimulates antimicrobial immunity. J Exp Med 207(11):2343-54. [PubMed: 20937702]  [MGI Ref ID J:166066]

Jehl SP; Doling AM; Giddings KS; Phalipon A; Sansonetti PJ; Goldberg MB; Starnbach MN. 2011. Antigen-Specific CD8+ T Cells Fail To Respond to Shigella flexneri. Infect Immun 79(5):2021-30. [PubMed: 21357720]  [MGI Ref ID J:171947]

Jolicoeur P; Hu C; Mak TW; Martinou JC; Kay DG. 2003. Protection against murine leukemia virus-induced spongiform myeloencephalopathy in mice overexpressing Bcl-2 but not in mice deficient for interleukin-6, inducible nitric oxide synthetase, ICE, Fas, Fas ligand, or TNF-R1 genes. J Virol 77(24):13161-70. [PubMed: 14645573]  [MGI Ref ID J:86761]

Kang TJ; Basu S; Zhang L; Thomas KE; Vogel SN; Baillie L; Cross AS. 2008. Bacillus anthracis spores and lethal toxin induce IL-1beta via functionally distinct signaling pathways. Eur J Immunol 38(6):1574-84. [PubMed: 18493980]  [MGI Ref ID J:136368]

Kayagaki N; Warming S; Lamkanfi M; Vande Walle L; Louie S; Dong J; Newton K; Qu Y; Liu J; Heldens S; Zhang J; Lee WP; Roose-Girma M; Dixit VM. 2011. Non-canonical inflammasome activation targets caspase-11. Nature 479(7371):117-21. [PubMed: 22002608]  [MGI Ref ID J:193522]

Kordes M; Matuschewski K; Hafalla JC. 2011. Caspase-1 Activation of Interleukin-1{beta} (IL-1{beta}) and IL-18 Is Dispensable for Induction of Experimental Cerebral Malaria. Infect Immun 79(9):3633-41. [PubMed: 21708993]  [MGI Ref ID J:175707]

Leibundgut-Landmann S; Weidner K; Hilbi H; Oxenius A. 2011. Nonhematopoietic cells are key players in innate control of bacterial airway infection. J Immunol 186(5):3130-7. [PubMed: 21270399]  [MGI Ref ID J:169394]

Li P; Allen H; Banerjee S; Franklin S; Herzog L; Johnston C; McDowell J; Paskind M; Rodman L; Salfeld J; Towne E; Tracey D; Wardwell S; Wei FY; Wong W; Kamen R; Seshadri T. 1995. Mice deficient in IL-1 beta-converting enzyme are defective in production of mature IL-1 beta and resistant to endotoxic shock. Cell 80(3):401-11. [PubMed: 7859282]  [MGI Ref ID J:22964]

Li P; Allen H; Banerjee S; Seshadri T. 1997. Characterization of mice deficient in interleukin-1 beta converting enzyme. J Cell Biochem 64(1):27-32. [PubMed: 9015751]  [MGI Ref ID J:40691]

Lichtnekert J; Kulkarni OP; Mulay SR; Rupanagudi KV; Ryu M; Allam R; Vielhauer V; Muruve D; Lindenmeyer MT; Cohen CD; Anders HJ. 2011. Anti-GBM Glomerulonephritis Involves IL-1 but Is Independent of NLRP3/ASC Inflammasome-Mediated Activation of Caspase-1. PLoS One 6(10):e26778. [PubMed: 22046355]  [MGI Ref ID J:178074]

Liege S; Moze E; Kelley KW; Parnet P; Neveu PJ. 2000. Activation of the hypothalamic-pituitary-adrenal axis in IL-1 beta-converting enzyme-deficient mice. Neuroimmunomodulation 7(4):189-94. [PubMed: 10810251]  [MGI Ref ID J:62433]

Lightfield KL; Persson J; Brubaker SW; Witte CE; von Moltke J; Dunipace EA; Henry T; Sun YH; Cado D; Dietrich WF; Monack DM; Tsolis RM; Vance RE. 2008. Critical function for Naip5 in inflammasome activation by a conserved carboxy-terminal domain of flagellin. Nat Immunol 9(10):1171-8. [PubMed: 18724372]  [MGI Ref ID J:141008]

Liu XH; Kwon D; Schielke GP; Yang GY; Silverstein FS; Barks JD. 1999. Mice deficient in interleukin-1 converting enzyme are resistant to neonatal hypoxic-ischemic brain damage. J Cereb Blood Flow Metab 19(10):1099-108. [PubMed: 10532634]  [MGI Ref ID J:98866]

Mankan AK; Canli O; Schwitalla S; Ziegler P; Tschopp J; Korn T; Greten FR. 2011. TNF-{alpha}-dependent loss of IKK{beta}-deficient myeloid progenitors triggers a cytokine loop culminating in granulocytosis. Proc Natl Acad Sci U S A 108(16):6567-72. [PubMed: 21464320]  [MGI Ref ID J:171365]

Mariathasan S; Weiss DS; Dixit VM; Monack DM. 2005. Innate immunity against Francisella tularensis is dependent on the ASC/caspase-1 axis. J Exp Med 202(8):1043-9. [PubMed: 16230474]  [MGI Ref ID J:116821]

Masters SL; Gerlic M; Metcalf D; Preston S; Pellegrini M; O'Donnell JA; McArthur K; Baldwin TM; Chevrier S; Nowell CJ; Cengia LH; Henley KJ; Collinge JE; Kastner DL; Feigenbaum L; Hilton DJ; Alexander WS; Kile BT; Croker BA. 2012. NLRP1 Inflammasome Activation Induces Pyroptosis of Hematopoietic Progenitor Cells. Immunity 37(6):1009-23. [PubMed: 23219391]  [MGI Ref ID J:191055]

Mastronardi C; Whelan F; Yildiz OA; Hannestad J; Elashoff D; McCann SM; Licinio J; Wong ML. 2007. Caspase 1 deficiency reduces inflammation-induced brain transcription. Proc Natl Acad Sci U S A 104(17):7205-10. [PubMed: 17409187]  [MGI Ref ID J:120877]

Meissner F; Molawi K; Zychlinsky A. 2008. Superoxide dismutase 1 regulates caspase-1 and endotoxic shock. Nat Immunol 9(8):866-72. [PubMed: 18604212]  [MGI Ref ID J:137865]

Miggin SM; Palsson-McDermott E; Dunne A; Jefferies C; Pinteaux E; Banahan K; Murphy C; Moynagh P; Yamamoto M; Akira S; Rothwell N; Golenbock D; Fitzgerald KA; O'Neill LA. 2007. NF-kappaB activation by the Toll-IL-1 receptor domain protein MyD88 adapter-like is regulated by caspase-1. Proc Natl Acad Sci U S A 104(9):3372-7. [PubMed: 17360653]  [MGI Ref ID J:125936]

Miwa K; Asano M; Horai R; Iwakura Y; Nagata S; Suda T. 1998. Caspase 1-independent IL-1beta release and inflammation induced by the apoptosis inducer Fas ligand. Nat Med 4(11):1287-92. [PubMed: 9809553]  [MGI Ref ID J:50775]

Moayeri M; Crown D; Newman ZL; Okugawa S; Eckhaus M; Cataisson C; Liu S; Sastalla I; Leppla SH. 2010. Inflammasome sensor Nlrp1b-dependent resistance to anthrax is mediated by caspase-1, IL-1 signaling and neutrophil recruitment. PLoS Pathog 6(12):e1001222. [PubMed: 21170303]  [MGI Ref ID J:168096]

Monack DM; Hersh D; Ghori N; Bouley D; Zychlinsky A; Falkow S. 2000. Salmonella exploits caspase-1 to colonize Peyer's patches in a murine typhoid model. J Exp Med 192(2):249-58. [PubMed: 10899911]  [MGI Ref ID J:63488]

Moreth K; Brodbeck R; Babelova A; Gretz N; Spieker T; Zeng-Brouwers J; Pfeilschifter J; Young MF; Schaefer RM; Schaefer L. 2010. The proteoglycan biglycan regulates expression of the B cell chemoattractant CXCL13 and aggravates murine lupus nephritis. J Clin Invest 120(12):4251-72. [PubMed: 21084753]  [MGI Ref ID J:171866]

Murphey ED. 2011. Cecal ligation and puncture-induced impairment of innate immune function does not occur in the absence of caspase-1. J Immunol 187(2):905-10. [PubMed: 21677131]  [MGI Ref ID J:178035]

Priceputu E; Rodrigue I; Chrobak P; Poudrier J; Mak TW; Hanna Z; Hu C; Kay DG; Jolicoeur P. 2005. The Nef-mediated AIDS-like disease of CD4C/human immunodeficiency virus transgenic mice is associated with increased Fas/FasL expression on T cells and T-cell death but is not prevented in Fas-, FasL-, tumor necrosis factor receptor 1-, or interleukin-1beta-converting enzyme-deficient or Bcl2-expressing transgenic mice. J Virol 79(10):6377-91. [PubMed: 15858021]  [MGI Ref ID J:98353]

Raupach B; Peuschel SK; Monack DM; Zychlinsky A. 2006. Caspase-1-mediated activation of interleukin-1beta (IL-1beta) and IL-18 contributes to innate immune defenses against Salmonella enterica serovar Typhimurium infection. Infect Immun 74(8):4922-6. [PubMed: 16861683]  [MGI Ref ID J:112400]

Rosenzweig HL; Galster KT; Planck SR; Rosenbaum JT. 2009. NOD1 expression in the eye and functional contribution to IL-1beta-dependent ocular inflammation in mice. Invest Ophthalmol Vis Sci 50(4):1746-53. [PubMed: 19074813]  [MGI Ref ID J:146667]

Rosenzweig HL; Martin TM; Planck SR; Galster K; Jann MM; Davey MP; Kobayashi K; Flavell RA; Rosenbaum JT. 2008. Activation of NOD2 in vivo induces IL-1beta production in the eye via caspase-1 but results in ocular inflammation independently of IL-1 signaling. J Leukoc Biol 84(2):529-36. [PubMed: 18495787]  [MGI Ref ID J:138433]

Rowe SJ; Allen L; Ridger VC; Hellewell PG; Whyte MK. 2002. Caspase-1-deficient mice have delayed neutrophil apoptosis and a prolonged inflammatory response to lipopolysaccharide-induced acute lung injury. J Immunol 169(11):6401-7. [PubMed: 12444148]  [MGI Ref ID J:80568]

Samardzija M; Wenzel A; Thiersch M; Frigg R; Reme C; Grimm C. 2006. Caspase-1 ablation protects photoreceptors in a model of autosomal dominant retinitis pigmentosa. Invest Ophthalmol Vis Sci 47(12):5181-90. [PubMed: 17122101]  [MGI Ref ID J:123100]

Sauer JD; Pereyre S; Archer KA; Burke TP; Hanson B; Lauer P; Portnoy DA. 2011. Listeria monocytogenes engineered to activate the Nlrc4 inflammasome are severely attenuated and are poor inducers of protective immunity. Proc Natl Acad Sci U S A 108(30):12419-24. [PubMed: 21746921]  [MGI Ref ID J:174533]

Schielke GP; Yang GY; Shivers BD; Betz AL. 1998. Reduced ischemic brain injury in interleukin-1 beta converting enzyme-deficient mice. J Cereb Blood Flow Metab 18(2):180-5. [PubMed: 9469161]  [MGI Ref ID J:46306]

Serbina NV; Hohl TM; Cherny M; Pamer EG. 2009. Selective expansion of the monocytic lineage directed by bacterial infection. J Immunol 183(3):1900-10. [PubMed: 19596996]  [MGI Ref ID J:151581]

Sundquist M; Wick MJ. 2005. TNF-alpha-dependent and -independent maturation of dendritic cells and recruited CD11c(int)CD11b+ Cells during oral Salmonella infection. J Immunol 175(5):3287-98. [PubMed: 16116221]  [MGI Ref ID J:113214]

Tang H; Cao W; Kasturi SP; Ravindran R; Nakaya HI; Kundu K; Murthy N; Kepler TB; Malissen B; Pulendran B. 2010. The T helper type 2 response to cysteine proteases requires dendritic cell-basophil cooperation via ROS-mediated signaling. Nat Immunol 11(7):608-17. [PubMed: 20495560]  [MGI Ref ID J:161857]

Trunk G; Oxenius A. 2012. Innate instruction of CD4+ T cell immunity in respiratory bacterial infection. J Immunol 189(2):616-28. [PubMed: 22723524]  [MGI Ref ID J:189793]

Vladimer GI; Weng D; Paquette SW; Vanaja SK; Rathinam VA; Aune MH; Conlon JE; Burbage JJ; Proulx MK; Liu Q; Reed G; Mecsas JC; Iwakura Y; Bertin J; Goguen JD; Fitzgerald KA; Lien E. 2012. The NLRP12 inflammasome recognizes Yersinia pestis. Immunity 37(1):96-107. [PubMed: 22840842]  [MGI Ref ID J:187388]

Wickstrum JR; Bokhari SM; Fischer JL; Pinson DM; Yeh HW; Horvat RT; Parmely MJ. 2009. Francisella tularensis induces extensive caspase-3 activation and apoptotic cell death in the tissues of infected mice. Infect Immun 77(11):4827-36. [PubMed: 19703976]  [MGI Ref ID J:154196]

Wong ML; Xie B; Beatini N; Phu P; Marathe S; Johns A; Gold PW; Hirsch E; Williams KJ; Licinio J; Tabas I. 2000. Acute systemic inflammation up-regulates secretory sphingomyelinase in vivo: A possible link between inflammatory cytokines and atherogenesis Proc Natl Acad Sci U S A 97(15):8681-6. [PubMed: 10890909]  [MGI Ref ID J:63405]

Xu H; Barks JD; Schielke GP; Silverstein FS. 2001. Attenuation of hypoxia-ischemia-induced monocyte chemoattractant protein-1 expression in brain of neonatal mice deficient in interleukin-1 converting enzyme. Brain Res Mol Brain Res 90(1):57-67. [PubMed: 11376856]  [MGI Ref ID J:69589]

Yang GY; Schielke GP; Gong C; Mao Y; Ge HL; Liu XH; Betz AL. 1999. Expression of tumor necrosis factor-alpha and intercellular adhesion molecule-1 after focal cerebral ischemia in interleukin-1beta converting enzyme deficient mice. J Cereb Blood Flow Metab 19(10):1109-17. [PubMed: 10532635]  [MGI Ref ID J:59704]

Yao JH; Ye SM; Burgess W; Zachary JF; Kelley KW; Johnson RW. 1999. Mice deficient in interleukin-1beta converting enzyme resist anorexia induced by central lipopolysaccharide. Am J Physiol 277(5 Pt 2):R1435-43. [PubMed: 10564217]  [MGI Ref ID J:58677]

Zhang WH; Wang X; Narayanan M; Zhang Y; Huo C; Reed JC; Friedlander RM. 2003. Fundamental role of the Rip2/caspase-1 pathway in hypoxia and ischemia-induced neuronal cell death. Proc Natl Acad Sci U S A 100(26):16012-7. [PubMed: 14663141]  [MGI Ref ID J:88212]

Zhao Y; Yang J; Shi J; Gong YN; Lu Q; Xu H; Liu L; Shao F. 2011. The NLRC4 inflammasome receptors for bacterial flagellin and type III secretion apparatus. Nature 477(7366):596-600. [PubMed: 21918512]  [MGI Ref ID J:177040]

de Bilbao F; Giannakopoulos P; Srinivasan A; Dubois-Dauphin M. 2000. In vivo study of motoneuron death induced by nerve injury in mice deficient in the caspase 1/ interleukin-1 beta-converting enzyme. Neuroscience 98(3):573-83. [PubMed: 10869851]  [MGI Ref ID J:118715]

van der Velden AW; Velasquez M; Starnbach MN. 2003. Salmonella rapidly kill dendritic cells via a caspase-1-dependent mechanism. J Immunol 171(12):6742-9. [PubMed: 14662878]  [MGI Ref ID J:118499]

Casp4^del related

Aachoui Y; Leaf IA; Hagar JA; Fontana MF; Campos CG; Zak DE; Tan MH; Cotter PA; Vance RE; Aderem A; Miao EA. 2013. Caspase-11 protects against bacteria that escape the vacuole. Science 339(6122):975-8. [PubMed: 23348507]  [MGI Ref ID J:193387]

Kayagaki N; Warming S; Lamkanfi M; Vande Walle L; Louie S; Dong J; Newton K; Qu Y; Liu J; Heldens S; Zhang J; Lee WP; Roose-Girma M; Dixit VM. 2011. Non-canonical inflammasome activation targets caspase-11. Nature 479(7371):117-21. [PubMed: 22002608]  [MGI Ref ID J:193522]

Masters SL; Gerlic M; Metcalf D; Preston S; Pellegrini M; O'Donnell JA; McArthur K; Baldwin TM; Chevrier S; Nowell CJ; Cengia LH; Henley KJ; Collinge JE; Kastner DL; Feigenbaum L; Hilton DJ; Alexander WS; Kile BT; Croker BA. 2012. NLRP1 Inflammasome Activation Induces Pyroptosis of Hematopoietic Progenitor Cells. Immunity 37(6):1009-23. [PubMed: 23219391]  [MGI Ref ID J:191055]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX11

Colony Maintenance

Mating System	Homozygote x Homozygote         (Female x Male)   01-MAR-06
Diet Information	LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

General Supply Notes

• Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	001976 NOD/ShiLtJ
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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phone:	207-288-6470
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JAX^® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

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In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.