Strain Name:

B6.129S1-Syt7tm1Nan/J

Stock Number:

004950

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
 
Donating Investigator Norma Andrews,   Yale University School of Medicine

Description
At birth, mice homozygous for the targeted allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Although Northern analysis fails to detect full-length transcripts, two minor alternatively spliced (alpha and beta) isoforms are generated. Protein product is not detected. Reproductive capacity appears to decline faster in mutant mice. The average litter size in +6 month-old mice is ~3 in mutants compared to ~7 in wild type mice. Lysosomal exocytosis and plasma membrane resealing in response to membrane wounding is impaired in mouse embryo fibroblasts derived from mutants. Histological analysis of mutants 14 weeks of age indicates that most tissues appear normal with the exception of enhanced accumulations of connective tissue elements, indicative of fibrosis, in skeletal muscle and skin. Examination of skeletal muscle from younger animals (4 weeks of age) reveals evidence of fiber degeneration and inflammation. Strong anti-nuclear antibody response is detected in serum at 19 and 44 weeks of age, but not in 8-week-old animals. Elevated levels of serum creatine kinase, a diagnostic marker for muscle fiber injury, are also observed. Decreased forelimb grip is observed in mutant mice more than 19 weeks of age. This mutant strain may be useful in studies examining the mechanisms of membrane homeostasis, secretion and autoimmunity.

Development
A targeting vector containing a loxP-flanked neomycin resistance was used to disrupt exons 4-5 of the targeted gene. This region encodes the C2A calcium binding domain. The construct was electroporated into 129S1/Sv-p+ Tyr+ KitlSl-J/+ derived W9.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Syt7
006388   B6;129-Syt7tm1Sud/J
006389   B6;129-Syt7tm2Sud/J
View Strains carrying other alleles of Syt7     (2 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Syt7tm1Nan/Syt7tm1Nan

        involves: 129S1/Sv * C57BL/6
  • cellular phenotype
  • abnormal cell physiology
    • embryo fibroblasts are resistant to invasion by Trypanosoma cruzi   (MGI Ref ID J:85201)
    • failure of exocytosis and wound resealing in embryo fibroblasts   (MGI Ref ID J:85201)
  • immune system phenotype
  • increased anti-nuclear antigen antibody level
    • antinuclear antibody response in serum by 19 weeks   (MGI Ref ID J:85201)
  • increased inflammatory response   (MGI Ref ID J:85201)
    • myositis
      • extensive endomysial cellular infiltration at week 4   (MGI Ref ID J:85201)
      • week 8, scattered foci of inflammation   (MGI Ref ID J:85201)
      • invasion of degenerating muscle fibers by macrophages and eosinophiles   (MGI Ref ID J:85201)
      • inflammatory cells less abundant as mice age but fibrosis detectable until week 44   (MGI Ref ID J:85201)
  • increased susceptibility to autoimmune disorder
    • similarities to human autoimmune polymyositis/dermatositis   (MGI Ref ID J:85201)
  • muscle phenotype
  • abnormal skeletal muscle morphology
    • extensive endomysial cellular infiltration at 4 weeks   (MGI Ref ID J:85201)
    • by 8 weeks, mast cells and bundles of collagen fibers found in endomysial space   (MGI Ref ID J:85201)
    • fiber degeneration   (MGI Ref ID J:85201)
    • skeletal muscle interstitial fibrosis
      • enhanced accumulation of connective tissue elements seen histologically in the skin and skeletal muscle (fibrosis)   (MGI Ref ID J:85201)
  • myositis
    • extensive endomysial cellular infiltration at week 4   (MGI Ref ID J:85201)
    • week 8, scattered foci of inflammation   (MGI Ref ID J:85201)
    • invasion of degenerating muscle fibers by macrophages and eosinophiles   (MGI Ref ID J:85201)
    • inflammatory cells less abundant as mice age but fibrosis detectable until week 44   (MGI Ref ID J:85201)
  • reproductive system phenotype
  • abnormal fertility/fecundity
    • reproductive capacity declines faster with age (litters of 3 at 6 months as opposed to 7 for wild-type mice)   (MGI Ref ID J:85201)
    • normal fertility otherwise   (MGI Ref ID J:85201)
  • behavior/neurological phenotype
  • decreased grip strength
    • decreased forelimb grip strength after 19 weeks   (MGI Ref ID J:85201)
  • integument phenotype
  • skin fibrosis
    • enhanced accumulation of connective tissue elements seen histologically in the skin and skeletal muscle (fibrosis)   (MGI Ref ID J:85201)
    • increased collagen deposition not seen in other tissues   (MGI Ref ID J:85201)
  • homeostasis/metabolism phenotype
  • skeletal muscle interstitial fibrosis
    • enhanced accumulation of connective tissue elements seen histologically in the skin and skeletal muscle (fibrosis)   (MGI Ref ID J:85201)
  • skin fibrosis
    • enhanced accumulation of connective tissue elements seen histologically in the skin and skeletal muscle (fibrosis)   (MGI Ref ID J:85201)
    • increased collagen deposition not seen in other tissues   (MGI Ref ID J:85201)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Syt7tm1Nan related

Immunology, Inflammation and Autoimmunity Research
Autoimmunity
      anti-nuclear antibodies
Inflammation

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Syt7tm1Nan
Allele Name targeted mutation 1, Norma W Andrews
Allele Type Targeted (Null/Knockout)
Common Name(s) Syt VII -;
Mutation Made By Norma Andrews,   Yale University School of Medicine
Strain of Origin129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
ES Cell Line NameW9.5/W95
ES Cell Line Strain129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
Gene Symbol and Name Syt7, synaptotagmin VII
Chromosome 19
Gene Common Name(s) AI851541; B230112P13Rik; IPCA-7; IPCA7; PCANAP7; RIKEN cDNA B230112P13 gene; SYT-VII; SYTVII; expressed sequence AI851541;
Molecular Note A loxP flanked neomycin resistance cassette replaced most of exon 4 and exon 5. In the process a stop codon was generated in exon 4 after the codon for amino acid 83. Absence of the 1.2kb mRNA for the major isoform of this gene was established by Northern blot analysis. Lack of protein product was confirmed by Western blot analysis. However, a truncated protein may be produced. [MGI Ref ID J:85201]

Genotyping

Genotyping Information

Genotyping Protocols

Syt7tm1Nan, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Syt7tm1Nan related

Chakrabarti S; Kobayashi KS; Flavell RA; Marks CB; Miyake K; Liston DR; Fowler KT; Gorelick FS; Andrews NW. 2003. Impaired membrane resealing and autoimmune myositis in synaptotagmin VII-deficient mice. J Cell Biol 162(4):543-9. [PubMed: 12925704]  [MGI Ref ID J:85201]

Chan WT; Sherer NM; Uchil PD; Novak EK; Swank RT; Mothes W. 2008. Murine leukemia virus spreading in mice impaired in the biogenesis of secretory lysosomes and Ca2+-regulated exocytosis. PLoS ONE 3(7):e2713. [PubMed: 18629000]  [MGI Ref ID J:139279]

Colvin RA; Means TK; Diefenbach TJ; Moita LF; Friday RP; Sever S; Campanella GS; Abrazinski T; Manice LA; Moita C; Andrews NW; Wu D; Hacohen N; Luster AD. 2010. Synaptotagmin-mediated vesicle fusion regulates cell migration. Nat Immunol 11(6):495-502. [PubMed: 20473299]  [MGI Ref ID J:160690]

Czibener C; Sherer NM; Becker SM; Pypaert M; Hui E; Chapman ER; Mothes W; Andrews NW. 2006. Ca2+ and synaptotagmin VII-dependent delivery of lysosomal membrane to nascent phagosomes. J Cell Biol 174(7):997-1007. [PubMed: 16982801]  [MGI Ref ID J:115013]

Fowler KT; Andrews NW; Huleatt JW. 2007. Expression and function of synaptotagmin VII in CTLs. J Immunol 178(3):1498-504. [PubMed: 17237398]  [MGI Ref ID J:143651]

Liu H; Bai H; Hui E; Yang L; Evans CS; Wang Z; Kwon SE; Chapman ER. 2014. Synaptotagmin 7 functions as a Ca2+-sensor for synaptic vesicle replenishment. Elife 3:e01524. [PubMed: 24569478]  [MGI Ref ID J:207968]

Zhao H; Ito Y; Chappel J; Andrews NW; Teitelbaum SL; Ross FP. 2008. Synaptotagmin VII regulates bone remodeling by modulating osteoclast and osteoblast secretion. Dev Cell 14(6):914-25. [PubMed: 18539119]  [MGI Ref ID J:137198]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryThis strain originated on a B6;129S1 background and has been backcrossed to C57BL/6 for 7 generations before being made homozygous. When maintained in a live colony, this strain is maintained by homozygous matings.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $1650.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $2145.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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