Description

Strain Information

Type Coisogenic; Mutant Strain; Spontaneous Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse

Important Note
This strain is homozygous for the retinal degeneration allele Pde6b^rd1.

Description
Mice homozygous for lethargic 3 Jackson are recognizable at 14 days of age. They exhibit a behavior characteristic of lethargic with a wobbly gait resembling an unsteady wide legged shuffle. Some Cacnb4^lh-3J homozygotes also have a stargazing phenotype, but seizures have not been reported. The homozygotes are smaller than their wildtype littermates but live a normal lifespan. Both sexes may breed but they produce smaller litters or stop breeding earlier than heterozygous sibs.

Development
The lethargic 3 Jackson mutation arose spontaneously in the C3HeB/FeJ colony at The Jackson Laboratory in the Spring of 2001 when that colony was at F182. After one backcross to C3HeB/FeJ, the strain was maintained by sibling mating, primarily homozygous female x heterozygous male since homozygous males do not tend to breed. In 2004 this strain reached F182+N1F6.

Control Information

	Control
	Heterozygote from the colony
	000658 C3HeB/FeJ
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Cacnb4

000504	B6EiC3Sn a/A-Cacnb4lh/J
008626	STOCK Cacnb4lh-2J/LetJ

View Strains carrying other alleles of Cacnb4     (2 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Cacnb4^lh-3J/Cacnb4^lh-3J

        C3HeB/FeJ

• behavior/neurological phenotype
• abnormal head movements (MGI Ref ID J:88414)
• abnormal locomotor activity (MGI Ref ID J:88414)
• abnormal gait (MGI Ref ID J:88414)
  • mice exhibit a behavior characteristic of other alleles of lethargic, with a wobbly gait resembling an unsteady wide legged shuffle
  • this phenotype is recognizable by 14 days of age
• lethargy (MGI Ref ID J:88414)
  • some lh-3J mice also have a stargazing phenotype, where their heads frequently point upwards, but seizures have not been reported
• growth/size phenotype
• decreased body size (MGI Ref ID J:88414)
  • homozygotes are smaller than their littermates but live a nornal lifespan

Genes & Alleles

Gene & Allele Information

Allele Symbol		Cacnb4lh-3J
Allele Name		lethargic 3 Jackson
Allele Type		Spontaneous
Strain of Origin		C3HeB/FeJ
Gene Symbol and Name		Cacnb4, calcium channel, voltage-dependent, beta 4 subunit
Chromosome		2
Gene Common Name(s)		3110038O15Rik; CAB4; CACNLB4; Cchb4; EA5; EJM; RIKEN cDNA 3110038O15 gene; calcium channel, B4 subunit; lethargic; lh;
Molecular Note		The allelic nature of this mouse to this locus was determined by complementation analysis with the original lh mouse. [MGI Ref ID J:88414]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Samples RM; Ward-Bailey P; Donahue LR; Bronson RT; Davisson MT. 2004. Lethargic 3 Jackson, a remutation of the Cacnb4 gene on mouse Chromosome 2. Mouse Mutant Resource Web Site, The Jackson Laboratory, Bar Harbor, Maine. World Wide Web (http://www.jax.org/mmr). MGI Direct Data Submission :.  [MGI Ref ID J:88414]

Original article from The Mouse Mutant Resource

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply	Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes	Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Mouse Mutant Resource collection. Genomic DNA is available for this strain from the Mouse DNA Resource.
Important Note	This strain is homozygous for the retinal degeneration allele Pde6brd1.

Control Information

	Control
	Heterozygote from the colony
	000658 C3HeB/FeJ
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
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Tel: 800.422.6423 or 207.288.5845
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Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

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phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

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MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.