Strain Name:

B6.129X1-Camk4tm1Tch/J

Stock Number:

004994

Order this mouse

Availability:

Cryopreserved - Ready for recovery

Other products are available, see Purchasing Information for Cryopreserved Embryos

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
 
Donating Investigator Talal Chatila,   Boston Children's Hospital

Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA or protein) is detected by RT-PCR or Western blot analysis. Synaptic plasticity, as assayed by long-term potentiation (LTP) and long-term depression (LTD), in mutant mice is deficient. Homozygotes exhibit diminished contextual and auditory fear memory. Cyclic AMP-responsive element binding protein (CREB) activation by fear conditioning is absent in the amygdala and decreased in the hippocampus. Thymocyte maturation is impaired. Both CD4+ and CD8+, single positive, thymocyte levels are reduced. This mutant mouse strain may be useful in studies examining learning and memory or related to thymocyte development.

Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exon 3 and adjacent introns. The construct was electroporated into 129X1/SvJ-derived RW4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts.

Control Information

  Control
   See control note: C57BL/6J mice (Stock No. 000664) may be used as controls.
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Camk4tm1Tch/Camk4tm1Tch

        B6.129X1-Camk4tm1Tch
  • behavior/neurological phenotype
  • abnormal learning/memory/conditioning
    • memory for increased vestibuloocular reflex gain is impaired compared to in wild-type mice regardless of spaced training   (MGI Ref ID J:122984)
    • however, vestibuloocular reflex, motor function and optokinetic reflex are normal and reduced training stimulus frequency rescues memory of increased vestibuloocular reflex gain   (MGI Ref ID J:122984)
    • impaired conditioned place preference behavior
      • mice spend less time exploring the morphine-paired side of the chamber than wild-type mice   (MGI Ref ID J:108062)
      • however, initial place preference and withdrawal behaviors are normal   (MGI Ref ID J:108062)
  • decreased chemically-elicited antinociception
    • mice exhibit less analgesic tolerance after chronic morphine treatment compared to wild-type mice   (MGI Ref ID J:108062)
  • skeleton phenotype
  • abnormal bone remodeling
    • LPS-induced bone erosion is reduced compared to in wild-type mice   (MGI Ref ID J:129599)
  • abnormal osteoclast differentiation
    • osteoclastognesis induced by RANKL is suppressed unlike in wild-type mice   (MGI Ref ID J:129599)
  • decreased osteoclast cell number   (MGI Ref ID J:129599)
  • increased bone mineral density   (MGI Ref ID J:129599)
  • increased bone volume
    • bone volume is increased compared to in wild-type mice   (MGI Ref ID J:129599)
  • nervous system phenotype
  • abnormal inhibitory postsynaptic currents
    • pre-exposure to morphine fails to abolish the morphine-induced decrease in inhibitory postsynaptic current as in wild-type mice   (MGI Ref ID J:108062)
  • immune system phenotype
  • abnormal osteoclast differentiation
    • osteoclastognesis induced by RANKL is suppressed unlike in wild-type mice   (MGI Ref ID J:129599)
  • decreased osteoclast cell number   (MGI Ref ID J:129599)
  • hematopoietic system phenotype
  • abnormal osteoclast differentiation
    • osteoclastognesis induced by RANKL is suppressed unlike in wild-type mice   (MGI Ref ID J:129599)
  • decreased osteoclast cell number   (MGI Ref ID J:129599)
  • integument phenotype
  • decreased chemically-elicited antinociception
    • mice exhibit less analgesic tolerance after chronic morphine treatment compared to wild-type mice   (MGI Ref ID J:108062)
  • cellular phenotype
  • abnormal osteoclast differentiation
    • osteoclastognesis induced by RANKL is suppressed unlike in wild-type mice   (MGI Ref ID J:129599)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Camk4tm1Tch/Camk4tm1Tch

        involves: 129X1/SvJ * C57BL/6
  • nervous system phenotype
  • *normal* nervous system phenotype
    • mice exhibit normal neuroanatomy   (MGI Ref ID J:64210)
    • abnormal Purkinje cell dendrite morphology
      • Purkinje neuron dendritic arbors appear reduced compared to wild-type neurons in culture   (MGI Ref ID J:64210)
    • abnormal excitatory postsynaptic currents
      • the frequency of miniature excitatory postsynaptic currents is reduced by 70% and the R input is increased by 250% compared to in wild-type mice   (MGI Ref ID J:64210)
    • reduced long term depression
      • long term depression is impaired   (MGI Ref ID J:64210)
  • behavior/neurological phenotype
  • *normal* behavior/neurological phenotype
    • mice exhibit normal spatial memory   (MGI Ref ID J:64210)
    • abnormal locomotor activation
      • while locomotion and sensorimotor functions are normal, mice take longer to initiate movement compared to wild-type mice   (MGI Ref ID J:64210)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Camk4tm1Tch related

Immunology, Inflammation and Autoimmunity Research
Immunodeficiency
      T cell deficiency

Internal/Organ Research
Lymphoid Tissue Defects
      T cell deficiency

Neurobiology Research
Behavioral and Learning Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Camk4tm1Tch
Allele Name targeted mutation 1, Talal A Chatila
Allele Type Targeted (knock-out)
Common Name(s) CaMKIV/Gr KO; Camk4-;
Mutation Made By Talal Chatila,   Boston Children's Hospital
Strain of Origin129X1/SvJ
ES Cell Line NameRW-4
ES Cell Line Strain129X1/SvJ
Gene Symbol and Name Camk4, calcium/calmodulin-dependent protein kinase IV
Chromosome 18
Gene Common Name(s) A430110E23Rik; AI666733; Ca2+/calmodulin-dependent protein kinase type IV/Gr; CaMK IV; CaMK-GR; CaMKIV; CaMKIV/Gr; Ccdpk; D18Bwg0362e; DNA segment, Chr 18, Brigham & Women's Genetics 0362 expressed; IV; RATCCDPK; RIKEN cDNA A430110E23 gene; caMK; expressed sequence AI666733;
Molecular Note The replacement of the third exon, which encodes the ATP binding site in the catalytic domain of the kinase, with a neomycin resistance gene rendered the enzyme catalytically inactive and prone to degradation. [MGI Ref ID J:64210]

Genotyping

Genotyping Information

Genotyping Protocols

Camk4tm1Tch, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Ho N; Liauw JA; Blaeser F; Wei F; Hanissian S; Muglia LM; Wozniak DF; Nardi A; Arvin KL; Holtzman DM; Linden DJ; Zhuo M; Muglia LJ; Chatila TA. 2000. Impaired synaptic plasticity and cAMP response element-binding protein activation in Ca2+/calmodulin-dependent protein kinase type IV/Gr-deficient mice. J Neurosci 20(17):6459-72. [PubMed: 10964952]  [MGI Ref ID J:64210]

Additional References

Raman V; Blaeser F; Ho N; Engle DL; Williams CB; Chatila TA. 2001. Requirement for Ca(2+)/Calmodulin-Dependent Kinase Type IV/Gr in Setting the Thymocyte Selection Threshold. J Immunol 167(11):6270-8. [PubMed: 11714790]  [MGI Ref ID J:72830]

Camk4tm1Tch related

Blaeser F; Toppari J; Heikinheimo M; Yan W; Wallace M; Ho N; Chatila TA. 2001. CaMKIV/Gr is dispensable for spermatogenesis and CREM-regulated transcription in male germ cells. Am J Physiol Endocrinol Metab 281(5):E931-7. [PubMed: 11595648]  [MGI Ref ID J:72562]

Boyden ES; Katoh A; Pyle JL; Chatila TA; Tsien RW; Raymond JL. 2006. Selective engagement of plasticity mechanisms for motor memory storage. Neuron 51(6):823-34. [PubMed: 16982426]  [MGI Ref ID J:122984]

Evankovich J; Zhang R; Cardinal JS; Zhang L; Chen J; Huang H; Beer-Stolz D; Billiar TR; Rosengart MR; Tsung A. 2012. Calcium/calmodulin-dependent protein kinase IV limits organ damage in hepatic ischemia-reperfusion injury through induction of autophagy. Am J Physiol Gastrointest Liver Physiol 303(2):G189-98. [PubMed: 22575222]  [MGI Ref ID J:191365]

Ichinose K; Rauen T; Juang YT; Kis-Toth K; Mizui M; Koga T; Tsokos GC. 2011. Cutting edge: Calcium/Calmodulin-dependent protein kinase type IV is essential for mesangial cell proliferation and lupus nephritis. J Immunol 187(11):5500-4. [PubMed: 22031763]  [MGI Ref ID J:179759]

Jackson KJ; Sanjakdar SS; Chen X; Damaj MI. 2012. Nicotine reward and affective nicotine withdrawal signs are attenuated in calcium/calmodulin-dependent protein kinase IV knockout mice. PLoS One 7(11):e51154. [PubMed: 23226481]  [MGI Ref ID J:195688]

Ko SW; Jia Y; Xu H; Yim SJ; Jang DH; Lee YS; Zhao MG; Toyoda H; Wu LJ; Chatila T; Kaang BK; Zhuo M. 2006. Evidence for a role of CaMKIV in the development of opioid analgesic tolerance. Eur J Neurosci 23(8):2158-68. [PubMed: 16630062]  [MGI Ref ID J:108062]

Koga T; Ichinose K; Mizui M; Crispin JC; Tsokos GC. 2012. Calcium/calmodulin-dependent protein kinase IV suppresses IL-2 production and regulatory T cell activity in lupus. J Immunol 189(7):3490-6. [PubMed: 22942433]  [MGI Ref ID J:190549]

Raman V; Blaeser F; Ho N; Engle DL; Williams CB; Chatila TA. 2001. Requirement for Ca(2+)/Calmodulin-Dependent Kinase Type IV/Gr in Setting the Thymocyte Selection Threshold. J Immunol 167(11):6270-8. [PubMed: 11714790]  [MGI Ref ID J:72830]

Sato K; Suematsu A; Nakashima T; Takemoto-Kimura S; Aoki K; Morishita Y; Asahara H; Ohya K; Yamaguchi A; Takai T; Kodama T; Chatila TA; Bito H; Takayanagi H. 2006. Regulation of osteoclast differentiation and function by the CaMK-CREB pathway. Nat Med 12(12):1410-6. [PubMed: 17128269]  [MGI Ref ID J:129599]

Wang H; Fukushima H; Kida S; Zhuo M. 2009. Ca2+/calmodulin-dependent protein kinase IV links group I metabotropic glutamate receptors to fragile X mental retardation protein in cingulate cortex. J Biol Chem 284(28):18953-62. [PubMed: 19436069]  [MGI Ref ID J:151259]

Wang H; Wu LJ; Zhang F; Zhuo M. 2008. Roles of calcium-stimulated adenylyl cyclase and calmodulin-dependent protein kinase IV in the regulation of FMRP by group I metabotropic glutamate receptors. J Neurosci 28(17):4385-97. [PubMed: 18434517]  [MGI Ref ID J:134617]

Wei F; Qiu CS; Liauw J; Robinson DA; Ho N; Chatila T; Zhuo M. 2002. Calcium calmodulin-dependent protein kinase IV is required for fear memory. Nat Neurosci 5(6):573-9. [PubMed: 12006982]  [MGI Ref ID J:76670]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2450.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $1600.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3185.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $2080.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   See control note: C57BL/6J mice (Stock No. 000664) may be used as controls.
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(6.6)