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Strain Name:

C3H-Tg(Camk2a-Creb1/ESR1)3Sva/J

Stock Number:

004995

Availability:

Repository-Cryopreserved

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Additional Licensing and Use Restrictions

Former Name      C3H-Tg(Camk2a-Creb1/Esr1)3Sva/J    (Changed: 15-DEC-04 )
Genes & Alleles   Camk2a;   Creb1;   ESR1;   Tg(Camk2a-Creb1/ESR1)3Sva;

Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Strain
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Transgenic
Specieslaboratory mouse
Donating Investigator Alcino Silva,   University of California, Los Angeles
GenerationN4p (30-JAN-05)

Strain Description
These transgenic mice have a tamoxifen inducible cAMP responsive element binding protein 1 (Creb1) repressor driven by the mouse calcium/calmodulin-dependent protein kinase II alpha promoter. The transgene insert contains a fusion product involving a mutant Creb1 isoform (CREBS133A) and a mutant form of the human estrogen receptor ligand binding domain (LBDG521R). Expression of the transgene is detected in the hippocampus and cortex as analyzed by Northern and Western blot. In addition, transgene expression is detected in the amygdala by RT-PCR analysis. The mutant human estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Tamoxifen administration activates the repressor, reducing cyclic AMP responsive element-mediated transcription. When the repressor system is activated, mutant mice exhibit impaired long term memory consolidation and retrieval in fear conditioning training. Publication observations were made on B6C3F1 animals. This mutant mouse strain may be useful in studies of the molecular basis for memory and learning.

Strain Development
A transgenic construct containing sequence encoding the CREBIR( cAMP responsive element binding protein 1 repressor/estrogen ligand-binding domain) fusion gene under the control of the mouse calcium/calmodulin-dependent protein kinase II alpha promoter and a polyadenylation site was introduced into C3H donor eggs.

Gene & Allele Details

Allele Symbol Tg(Camk2a-Creb1/ESR1)3Sva
Allele Name transgene insertion 3, Alcino Silva
Common Name(s) CREB-IR; CREBIR; line C;
Mutation Made By Rachel Chen,   University of California, Los Angeles
Strain of OriginC3H/HeNTac
Expressed Gene Creb1, cAMP responsive element binding protein 1, mouse, laboratory
Expressed Gene ESR1, estrogen receptor 1, human
Promoter Camk2a, calcium/calmodulin-dependent protein kinase II alpha, mouse, laboratory
General Note In transgenic mice tamoxifen administration causes a reduction in cyclic AMP responsive element-mediated transcription These mice tehn exhibit impaired long term memory consolidation and retrieval in fear conditioning training.
Molecular Note The transgene expresses a fusion protein comprising the tamoxifen-, but not estrogen-inducible G521R mutant of the human estrogen receptor ligand binding domain joined to the transcriptionally inactive S133A mutant of cAMP responsive element binding protein 1 (CREB1), which competitively inhibits binding of native CREB1 to cAMP response elements, under control of the promoter of mouse calcium/calmodulin-dependent protein kinase II alpha, which in the adult animal directs expression to the excitatory neurons of forebrain regions including the hippocampus, amygdala, cortex and striatum. Transcription of the fusion gene in cortex, hippocampus and striatum of transgenic mice was confirmed by reverse transcription followed by polymerase chain reaction (RT-PCR). RT-PCR analysis detected transgene expression in the amygdala. Northern and Western blot analyses detected expression of the transgene in the hippocampus and cortex. [MGI Ref ID J:75643]

Control Information

   
   Wildtype mice from the colony or C3H/HeJ (Stock No. 000659) may be used as controls.
 
  Considerations for Choosing Controls

Genotyping Protocols

Tg(Camk2a-Creb1/Esr1)3Sva

Colony Maintenance

Breeding & HusbandryThe resulting male transgenic mice were backcrossed for 5 generations on the C3H background. The strain is maintained as a hemizygote.

Related Strains

View Strains carrying other alleles of Camk2a     (10 strains)

Strains carrying other alleles of Creb1
004445   B6.129S2-Creb1tm1Gsc/J
View Strains carrying other alleles of Creb1     (1 strain)

View Strains carrying other alleles of ESR1     (7 strains)

Research Applications

This mouse can be used to support research in many areas including:

Developmental Biology Research
Embryonic Lethality (Homozygous) (incomplete)
Internal/Organ Defects (ovary; uterus)

Internal/Organ Research
Other Organ Defects

Neurobiology Research
Behavioral and Learning Defects

Reproductive Biology Research
Developmental Defects Affecting Gonads (germ cell deficient)
Fertility Defects

References

Selected Reference(s)

Kida S; Josselyn SA; de Ortiz SP; Kogan JH; Chevere I; Masushige S; Silva AJ. 2002. CREB required for the stability of new and reactivated fear memories. Nat Neurosci 5(4):348-55. [PubMed: 11889468]  [MGI Ref ID J:75643]

Additional References

Price and Supply Information

Strain Name: C3H-Tg(Camk2a-Creb1/ESR1)3Sva/J
Stock Number: 004995

Price Details

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Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
This strain is included in the Induced Mutant Resource Colony collection.

LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

View JAX® Mice & Services Conditions of Use.

Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.

For additional Licensing and Use Restrictions view the link(s) below:
- Notice Regarding cre/Esr1 (Tamoxifen Inducible Cre) Strains.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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