Strain Name:

C3H-Tg(Camk2a-Creb1/ESR1)3Sva/J

Stock Number:

004995

Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names C3H-Tg(Camk2a-Creb1/Esr1)3Sva/J    (Changed: 15-DEC-04 )
Type Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
GenerationN4p (30-JAN-05)
 
Donating Investigator Alcino Silva,   University of California, Los Angeles

Description
These transgenic mice have a tamoxifen inducible cAMP responsive element binding protein 1 (Creb1) repressor driven by the mouse calcium/calmodulin-dependent protein kinase II alpha promoter. The transgene insert contains a fusion product involving a mutant Creb1 isoform (CREBS133A) and a mutant form of the human estrogen receptor ligand binding domain (LBDG521R). Expression of the transgene is detected in the hippocampus and cortex as analyzed by Northern and Western blot. In addition, transgene expression is detected in the amygdala by RT-PCR analysis. The mutant human estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Tamoxifen administration activates the repressor, reducing cyclic AMP responsive element-mediated transcription. When the repressor system is activated, mutant mice exhibit impaired long term memory consolidation and retrieval in fear conditioning training. Publication observations were made on B6C3F1 animals. This mutant mouse strain may be useful in studies of the molecular basis for memory and learning.

Development
A transgenic construct containing sequence encoding the CREBIR( cAMP responsive element binding protein 1 repressor/estrogen ligand-binding domain) fusion gene under the control of the mouse calcium/calmodulin-dependent protein kinase II alpha promoter and a polyadenylation site was introduced into C3H donor eggs.

Control Information

   
   Wildtype mice from the colony or C3H/HeJ (Stock No. 000659) may be used as controls.
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of Camk2a     (11 strains)

Strains carrying other alleles of Creb1
004445   B6.129S2-Creb1tm1Gsc/J
View Strains carrying other alleles of Creb1     (1 strain)

View Strains carrying other alleles of ESR1     (13 strains)

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Developmental Biology Research
Embryonic Lethality (Homozygous)
      incomplete
Internal/Organ Defects
      ovary; uterus

Internal/Organ Research
Other Organ Defects

Neurobiology Research
Behavioral and Learning Defects

Reproductive Biology Research
Developmental Defects Affecting Gonads
      germ cell deficient
Fertility Defects

Genes & Alleles

Gene & Allele Information

 
Allele Symbol Tg(Camk2a-Creb1/ESR1)3Sva
Allele Name transgene insertion 3, Alcino Silva
Allele Type Transgenic (random, expressed)
Common Name(s) CREB-IR; CREBIR; line C;
Mutation Made By Rachel Chen,   University of California, Los Angeles
Strain of OriginC3H/HeNTac
Expressed Gene ESR1, estrogen receptor 1, human
Expressed Gene Creb1, cAMP responsive element binding protein 1, mouse, laboratory
Promoter Camk2a, calcium/calmodulin-dependent protein kinase II alpha, mouse, laboratory
General Note In transgenic mice tamoxifen administration causes a reduction in cyclic AMP responsive element-mediated transcription These mice tehn exhibit impaired long term memory consolidation and retrieval in fear conditioning training.
Molecular Note The transgene expresses a fusion protein comprising the tamoxifen-, but not estrogen-inducible G521R mutant of the human estrogen receptor ligand binding domain joined to the transcriptionally inactive S133A mutant of cAMP responsive element binding protein 1 (CREB1), which competitively inhibits binding of native CREB1 to cAMP response elements, under control of the promoter of mouse calcium/calmodulin-dependent protein kinase II alpha, which in the adult animal directs expression to the excitatory neurons of forebrain regions including the hippocampus, amygdala, cortex and striatum. Transcription of the fusion gene in cortex, hippocampus and striatum of transgenic mice was confirmed by reverse transcription followed by polymerase chain reaction (RT-PCR). RT-PCR analysis detected transgene expression in the amygdala. Northern and Western blot analyses detected expression of the transgene in the hippocampus and cortex. [MGI Ref ID J:75643]
 
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(Camk2a-Creb1/Esr1)3Sva, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Kida S; Josselyn SA; de Ortiz SP; Kogan JH; Chevere I; Masushige S; Silva AJ. 2002. CREB required for the stability of new and reactivated fear memories. Nat Neurosci 5(4):348-55. [PubMed: 11889468]  [MGI Ref ID J:75643]

Additional References

Tg(Camk2a-Creb1/ESR1)3Sva related

Josselyn SA; Kida S; Silva AJ. 2004. Inducible repression of CREB function disrupts amygdala-dependent memory. Neurobiol Learn Mem 82(2):159-63. [PubMed: 15341801]  [MGI Ref ID J:128870]

Mamiya N; Fukushima H; Suzuki A; Matsuyama Z; Homma S; Frankland PW; Kida S. 2009. Brain region-specific gene expression activation required for reconsolidation and extinction of contextual fear memory. J Neurosci 29(2):402-13. [PubMed: 19144840]  [MGI Ref ID J:144498]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Colony Maintenance

Breeding & HusbandryThe resulting male transgenic mice were backcrossed for 5 generations on the C3H background. The strain is maintained as a hemizygote.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Price (US dollars $)
Cryorecovery Fee $1900.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Price (US dollars $)
Cryorecovery Fee $2470.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Additional Supply Details

Supply Details

Standard SupplyCryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery - Standard.
    We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

  • This strain is included in the Induced Mutant Resource Colony collection.

Control Information

   
   Wildtype mice from the colony or C3H/HeJ (Stock No. 000659) may be used as controls.
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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