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Strain Name:

B6.Cg-Tg(DBH-Gal)1923Stei/J

Stock Number:

004996

Availability:

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General Terms and Conditions

Genes & Alleles   DBH;   Gal;   Tg(DBH-Gal)1923Stei;

Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Transgenic
Specieslaboratory mouse
Donating Investigator Robert Steiner,   University of Washington, Box 357290
GenerationN8F1N1+N1p (20-NOV-05)

Strain Description
These transgenic mice overexpress the mouse galanin protein under the direction of the human dopamine beta-hydroxylase promoter. Mice that are homozygous for the transgene are viable, normal in size and do not display any gross physical or behavioral abnormalities. In situ hybridization analysis of brain detects a 5-fold increase in galanin mRNA levels in the locus coeruleus region. Radioimmunoassay reveals a 2-fold increase in gene product peptide level in the forebrain. An increase of galanin-like immunoreactive staining and fiber density is also detected. The number of basal forebrain cholinergic neurons is decreased. Transgenic mice have impaired learning and memory as assessed by the Morris spatial navigational test and the social transmission of food preference test. Resistance to seizures induced by perforant path stimulation or administration of kainic acid is increased. Mutant mice exhibit increased afterdischarge (AD) induction threshold and diminished glutamate release after depolarization. Treatment with M35, a galanin receptor antagonist, rescues abnormal seizure response. Perforant path-dentate gyrus long-term potentiation is impaired. This mutant mouse strain represents a model that may be useful in studies of Alzheimer's disease and epileptic seizures.

Strain Development
A transgenic construct containing a 5.8 Kb sequence of human dopamine beta-hydroxylase promoter, and a 4.6 Kb sequence of the mouse galanin gene was injected into fertilized SJL X C57BL/6 mouse eggs. The resulting male transgenic mice were backcrossed for 7 generations on the C57BL/6J background.

Mammalian Phenotype Terms assigned by genotype

Tg(DBH-Gal)1923Stei/0

        B6.Cg-Tg(DBH-Gal)1923Stei
  • life span-post-weaning/aging
  • abnormal induced morbidity/mortality (MGI Ref ID J:77625)
    • 1/6 mice die after 30 mg/kg kainic acid treatment compared to 5/6 wild-type mice
  • behavior/neurological phenotype
  • abnormal olfactory -discrimination memory (MGI Ref ID J:102197)
    • transgenic mice show no preference for a previously cued food odor 24 hours after exposure, whereas wild-type show significant preference for cued food odor
  • abnormal spatial learning (MGI Ref ID J:102197)
    • mice show significant impairment compared to controls, failing to display selective search during the probe trial when the platform is removed in the Morris water maze; controls show greater preference for the target quadrant at 8 and 16 months
    • mutants have normal performance in the visible and hidden platform trials, but show inability to remember navigational cues needed to solve spatial navigation problem
  • seizures (MGI Ref ID J:77625)
    • after 60 minutes of perforant path stimulation, only brief seizure activity is seen in transgenic mice compared to wild-type controls; last seizure occurs 25 minutes after PPS
    • resistance to pharmacologically induced seizures (MGI Ref ID J:77625)
      • 30 mg/kg kainic acid treatment induces a few stage 5 seizures, resulting in death of 1/6 animals, but wild-type animals exhibit severe seizures, with death occurring in 5/6 animals within 30 minutes
      • pentylene tetrazol (PTZ) treatment results in less severe seizures than those induced by kainic acid
      • galanin agonist treatment reduces the latency and severity of PTZ-induced seizures
  • nervous system phenotype
  • abnormal cholinergic neuron morphology (MGI Ref ID J:102197)
    • disappearance of detectable cholinergic neurons is observed
  • abnormal hippocampus function (MGI Ref ID J:77625)
    • mice have significantly higher afterdischarge threshold compared to wild-type mice
  • abnormal hippocampus morphology (MGI Ref ID J:77625)
    • after kainic acid treatment, the surviving transgenic mice display mild injury to the hippocampus, compared to the wild-type survivor which displayed moderate injury in the CA1, CA3, and hilus
  • reduced long term potentiation (MGI Ref ID J:77625)
    • mutants show faster LTP decay compared to wild type
  • seizures (MGI Ref ID J:77625)
    • after 60 minutes of perforant path stimulation, only brief seizure activity is seen in transgenic mice compared to wild-type controls; last seizure occurs 25 minutes after PPS
    • resistance to pharmacologically induced seizures (MGI Ref ID J:77625)
      • 30 mg/kg kainic acid treatment induces a few stage 5 seizures, resulting in death of 1/6 animals, but wild-type animals exhibit severe seizures, with death occurring in 5/6 animals within 30 minutes
      • pentylene tetrazol (PTZ) treatment results in less severe seizures than those induced by kainic acid
      • galanin agonist treatment reduces the latency and severity of PTZ-induced seizures
  • homeostasis/metabolism phenotype
  • resistance to pharmacologically induced seizures (MGI Ref ID J:77625)
    • 30 mg/kg kainic acid treatment induces a few stage 5 seizures, resulting in death of 1/6 animals, but wild-type animals exhibit severe seizures, with death occurring in 5/6 animals within 30 minutes
    • pentylene tetrazol (PTZ) treatment results in less severe seizures than those induced by kainic acid
    • galanin agonist treatment reduces the latency and severity of PTZ-induced seizures

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Tg(DBH-Gal)1923Stei/0

        involves: C57BL/6 * SJL
  • behavior/neurological phenotype
  • increased thermal nociceptive threshold (MGI Ref ID J:100969)
    • paw withdrawal and tail flick latency are elevated compared to wild-type controls in response to radiant heat
  • touch/vibrissae phenotype
  • increased thermal nociceptive threshold (MGI Ref ID J:100969)
    • paw withdrawal and tail flick latency are elevated compared to wild-type controls in response to radiant heat

Gene & Allele Details

Allele Symbol Tg(DBH-Gal)1923Stei
Allele Name transgene insertion 1923, Robert A Steiner
Common Name(s) GALANIN-Tg; GALTG; GalOE; GalOE/D;
Mutation Made By Karen Hansen,   University of Washington
Strain of Origin(SJL x C57BL/6)F1
Expressed Gene Gal, galanin, mouse, laboratory
Promoter DBH, dopamine beta-hydroxylase (dopamine beta-monooxygenase), human
Molecular Note The transgene contains a 5.8 Kb sequence of human dopamine beta-hydroxylase promoter and a 4.6 Kb sequence of the mouse galanin gene. In situ hybridization analysis of brain detects a 5-fold increase in galanin mRNA levels in the locus coeruleus region. Radioimmunoassay reveals a 2-fold increase in gene product peptide level in the forebrain. An increase of galanin-like immunoreactive staining and fiber density is also detected. [MGI Ref ID J:77625]

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Genotyping Protocols

Tg(DBH-Gal)1923Stei

Colony Maintenance

Breeding & HusbandryThis transgenic strain originated on a B6;SJL background, was backcrossed for 7 generations on the C57BL/6J background.
Diet Information LabDiet® 5K52/5K67

Related Strains

Strains carrying other alleles of DBH
003139   B6.Cg-Tg(DBHn-lacZ)8Rpk/J
View Strains carrying other alleles of DBH     (1 strain)

Additional Web Information

Congenic Nomenclature
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.

Research Applications

This mouse can be used to support research in many areas including:

Neurobiology Research
Alzheimer's Disease
Epilepsy

References

Selected Reference(s)

Mazarati AM; Hohmann JG; Bacon A; Liu H; Sankar R; Steiner RA; Wynick D; Wasterlain CG. 2000. Modulation of hippocampal excitability and seizures by galanin. J Neurosci 20(16):6276-81. [PubMed: 10934278]  [MGI Ref ID J:77625]

Additional References

Price and Supply Information

Strain Name: B6.Cg-Tg(DBH-Gal)1923Stei/J
Stock Number: 004996

Price Details

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Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.

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Control InformationView Control Information in Strain Details.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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