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Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation ?+N1p (30-JAN-05) Donating Investigator Harold Moses, Vanderbilt-Ingram Cancer Center Description
These transgenic mice express a truncated human transforming growth factor, beta receptor II under the direction of the mouse mammary tumor virus promoter. The truncated human transforming growth factor, beta receptor II has defective kinase activity. The transgene (mRNA) is detected at low levels in mammary gland tissue of 13-15 week old mice and at higher levels in mice aged 18 to 23 weeks, by Northern blot analysis. In situ hybridization shows expression is localized to mammary gland epithelial cells of terminal ducts and alveolar buds. At 20 weeks of age, virgin females develop mammary epithelial hyperplasia and increased secretion of beta casein protein, characteristic of early to mid-pregnancy. Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Half of all homozygous females are unable to feed pups. This mutant mouse strain may be useful in studies examining the induction of hyperplasia in mammary epithelial tissue.Development
A transgenic construct containing sequence encoding a truncated human TGFBR2 gene under the direction of the mouse mammary tumor virus LTR promoter/enhancer, and exons 2 and 3 of the rabbit beta-globin gene was injected into B6DBAF2 fertilized eggs.
| Wildtype mice from the colony or C57BL/6J mice (Stock No. 000664) may be used as controls. | ||
| Considerations for Choosing Controls | ||
Strains carrying other alleles of MMTV
002618 B6.Cg-Tg(MMTVtTA)1Mam/J 007962 B6.FVB-Tg(MMTV-neu/OT-I/OT-II)CBnel Tg(Trp53R172H)8512Jmr/J 002375 B6;D2-Tg(MMTVTGFB1)46Hlm/J 010576 B6;SJL-Tg(MMTV-rtTA)4-1Jek/J 002459 B6D2-Tg(MMTVTGFA)254Rjc/J 002373 B6D2-Tg(MMTVTGFA)29Rjc/J 002870 B6SJL-Tg(Wnt1)1Hev/J 005038 FVB-Tg(MMTV-Erbb2)NK1Mul/J 004363 FVB.Cg-Tg(MMTV-vHaras)SH1Led/J 002953 FVB.Cg-Tg(MMTVTGFA)254Rjc/J 002934 FVB.Cg-Tg(Wnt1)1Hev/J 002437 FVB/N-Tg(MMTV-Notch4)3Rnc/J 002374 FVB/N-Tg(MMTV-PyVT)634Mul/J 002376 FVB/N-Tg(MMTVneu)202Mul/J 002933 FVB/NJ-Tg(MMTVTGFB1)46Hlm/J 003690 STOCK Tg(MMTV-Cdc37)1Stp/J 003337 STOCK Tg(MMTV-PIP)1Shu/J 003551 STOCK Tg(MMTV-cre)1Mam/J 003553 STOCK Tg(MMTV-cre)4Mam/J View Strains carrying other alleles of MMTV (19 strains)
View Related Disease (OMIM) Terms
View Mammalian Phenotype Terms
Mammalian Phenotype Terms
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(MMTV-TGFBR2)7Hlm/0
involves: C57BL/6 * DBA
- endocrine/exocrine gland phenotype
- abnormal lactation (MGI Ref ID J:46432)
- the milk protein beta-Casein is detectable in the alveolar buds of non-pregnant females
- mammary gland alveolar hyperplasia (MGI Ref ID J:46432)
- alveolar hyperplasia is evident in non-pregnant females while no alveolar structures are observed in non-pregnant wild-type mice
- the alveolar-like buds fill in the spaces between the ductal structure of the mammary gland
- the morphology of the mammary gland resembles the morphology found in pregnant wild-type mice at early to mid-gestation
- the hyperplasia observed is less severe than observed in mice homozygous for the transgene
- reproductive system phenotype
- abnormal lactation (MGI Ref ID J:46432)
- the milk protein beta-Casein is detectable in the alveolar buds of non-pregnant females
- mammary gland alveolar hyperplasia (MGI Ref ID J:46432)
- alveolar hyperplasia is evident in non-pregnant females while no alveolar structures are observed in non-pregnant wild-type mice
- the alveolar-like buds fill in the spaces between the ductal structure of the mammary gland
- the morphology of the mammary gland resembles the morphology found in pregnant wild-type mice at early to mid-gestation
- the hyperplasia observed is less severe than observed in mice homozygous for the transgene
Tg(MMTV-TGFBR2)7Hlm/Tg(MMTV-TGFBR2)7Hlm
involves: C57BL/6 * DBA
- endocrine/exocrine gland phenotype
- abnormal involution of the mammary gland (MGI Ref ID J:85799)
- there is consistent retardation of involution observed in mice both 7 and 10 days after weaning compared to control mice
- abnormal lactation (MGI Ref ID J:46432)
- the milk protein beta-Casein is detectable in the alveolar buds of non-pregnant females
- the concentration and location of the beta-Casein is similar to what is found in 12.5-day pregnant females
- mice are frequently unable to feed their pups
- while alveolar hyperplasia with some milk production occurs early in pregnancy (15 days), the mammary glands fail to develop into fully functional organs as indicated by less extensive alveolar development and decreased milk production compared to non-transgenic controls
- abnormal mammary gland growth during lactation (MGI Ref ID J:85799)
- there is accelerated development and differentiation of mammary epithelial cells during early to mid-pregnancy but impaired development during the last stages of pregnancy
- mammary gland alveolar hyperplasia (MGI Ref ID J:46432)
- alveolar hyperplasia is evident in non-pregnant females while no alveolar structures are observed in non-pregnant wild-type mice
- the alveolar-like buds fill in the spaces between the ductal structure of the mammary gland
- the morphology of the mammary gland resembles the morphology found in pregnant wild-type mice at early to mid-gestation
- the hyperplasia observed is more severe than observed in mice hemizyguos for the transgene
- mice 15 days into their first pregnancy have more prominent vacuoles in the alveoli than in control mice
- the alveolar are also in a more differentiated state as indicated by the presence of milk proteins in the lumen
- mammary gland hypoplasia (MGI Ref ID J:85799)
- mice have fewer alveoli 1 day after the onset of lactation than do control mice
- reproductive system phenotype
- abnormal involution of the mammary gland (MGI Ref ID J:85799)
- there is consistent retardation of involution observed in mice both 7 and 10 days after weaning compared to control mice
- abnormal lactation (MGI Ref ID J:46432)
- the milk protein beta-Casein is detectable in the alveolar buds of non-pregnant females
- the concentration and location of the beta-Casein is similar to what is found in 12.5-day pregnant females
- mice are frequently unable to feed their pups
- while alveolar hyperplasia with some milk production occurs early in pregnancy (15 days), the mammary glands fail to develop into fully functional organs as indicated by less extensive alveolar development and decreased milk production compared to non-transgenic controls
- abnormal mammary gland growth during lactation (MGI Ref ID J:85799)
- there is accelerated development and differentiation of mammary epithelial cells during early to mid-pregnancy but impaired development during the last stages of pregnancy
- mammary gland alveolar hyperplasia (MGI Ref ID J:46432)
- alveolar hyperplasia is evident in non-pregnant females while no alveolar structures are observed in non-pregnant wild-type mice
- the alveolar-like buds fill in the spaces between the ductal structure of the mammary gland
- the morphology of the mammary gland resembles the morphology found in pregnant wild-type mice at early to mid-gestation
- the hyperplasia observed is more severe than observed in mice hemizyguos for the transgene
- mice 15 days into their first pregnancy have more prominent vacuoles in the alveoli than in control mice
- the alveolar are also in a more differentiated state as indicated by the presence of milk proteins in the lumen
- mammary gland hypoplasia (MGI Ref ID J:85799)
- mice have fewer alveoli 1 day after the onset of lactation than do control mice
- tumorigenesis
- mammary adenocarcinoma (MGI Ref ID J:85799)
- mice that have experienced two rounds of pregnancy have a frequency of spontaneous mammary carcinoma
- 47% of these females develop mammary tumors with a median latency of 27.5 months
- 72% of these tumors are high grade mammary intraepithelial neoplasias
- the remaining 28% are invasive adenocarcinomas or adenosquamous carcinomas
View Research Applications
Research Applications
This mouse can be used to support research in many areas including:
Cancer Research
| Allele Symbol | Tg(MMTV-TGFBR2)7Hlm | ||
|---|---|---|---|
| Allele Name | transgene insertion 7, Harold L Moses | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | MMTV-DNIIR-7; | ||
| Mutation Made By | Agnieszka Gorska, Vanderbilt Univ Schl of Medicine | ||
| Strain of Origin | (C57BL/6 x DBA)F2 | ||
| Expressed Gene | TGFB2, transforming growth factor, beta 2, human | ||
| Promoter | MMTV, Mouse Mammary Tumor Virus, MMTV | ||
| Molecular Note | A human cDNA encoding a carboxy-terminally truncated TGFBR2 was adjoined to an MMTV regulatory region. While the encoded receptor contains the complete extracellular and transmembrane domains and is therefore capable of ligand binding and and interactingwith type 1 receptors, it lacks the kinase domain. Expression was driven in mammary epithelial cells by the MMTV LTR promoter/enhancer. Transgenic expression was verified by Northern blot analysis. [MGI Ref ID J:46432] | ||
Genotyping Protocols
Tg(MMTV-TGFBR2)7Hlm, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
Gorska AE; Joseph H; Derynck R; Moses HL; Serra R. 1998. Dominant-negative interference of the transforming growth factor beta type II receptor in mammary gland epithelium results in alveolar hyperplasia and differentiation in virgin mice. Cell Growth Differ 9(3):229-38. [PubMed: 9543389] [MGI Ref ID J:46432]
Tg(MMTV-TGFBR2)7Hlm relatedGorska AE; Jensen RA; Shyr Y; Aakre ME; Bhowmick NA; Moses HL. 2003. Transgenic mice expressing a dominant-negative mutant type II transforming growth factor-beta receptor exhibit impaired mammary development and enhanced mammary tumor formation. Am J Pathol 163(4):1539-49. [PubMed: 14507660] [MGI Ref ID J:85799]
Colony Maintenance
Breeding & Husbandry The resulting transgenic mice were backcrossed for 20 generations on the C57BL/6 background. The strain is maintained as a homozygote. Surrogate mothers are used because 50% homozygous females cannot feed pups.
| Pricing for USA, Canada and Mexico shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 Cryopreserved Embryos $1600.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
| Pricing for International shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 Cryopreserved Embryos $2080.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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| Wildtype mice from the colony or C57BL/6J mice (Stock No. 000664) may be used as controls. | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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