Strain Name:

ZRDCT Raxey1/ChUmdJ

Stock Number:

005004

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names ZRDCT Raxey1/ChUmiJ    (Changed: 15-DEC-04 )
Type Coisogenic; Mutant Strain; Spontaneous Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse

Description
Ninety percent of mice homozygous for Raxey1 are anophthalmic and 10% are microphthalmic. Anophthalmic mice have orbits and eyelids but lack eyes and optic tracts (Chase et al. 1941). Microphthalmic mice have one or two small eyes, and may have an optic nerve (Chase et al. 1941). The phenotype is first distinguished at approximately embryonic day ten. Optic vesicles develop, however, they are reduced in size and contact poorly with surface ectoderm (Chase et al. 1941). A normal eyed control strain, ZRDCT Rax+/ChUmdJ (Stock No. 005005) was developed in parallel with ZRDCT Raxey1. Raxey1 mice exhibit an abnormal hypothalmus (Silver 1977) and altered circadian rhythm (Laemle et al. 1998). This strain serves as a model for human anophthalmia.

Development
ZRDCT Raxey1 and ZRDCT Rax+ mice were developed from the anophthalmic strain B described by Chase in 1941. The original animals from strain R were obtained from Little at The Jackson laboratory in 1938. Little selectively bred for anophthalmia by sibling matings, Chase continued the inbreeding to 21+ generations. Two sublines were established designated Be1 for the 98% eyeless group (Raxey1) and Be2 for the 96% normal group (Rax+) (Chase 1944). The donating investigator, Dr. Lois Laemle, indicates that this strain came from Chase through Dr. Kaiserman-Abramof. This strain did not come from Dr. Sidney Beck. It is not clear when the designation ZRDCT appeared. The strain was donated to The Jackson Laboratory in 2004.

Control Information

  Control
   005005 ZRDCT Rax+/ChUmdJ
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Rax
005005   ZRDCT Rax+/ChUmdJ
View Strains carrying other alleles of Rax     (1 strain)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Microphthalmia, Isolated 3; MCOP3   (RAX)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Raxey1/Rax+

        involves: ZRDCT/Ch
  • vision/eye phenotype
  • abnormal eye morphology
    • mice exhibit a higher incidence of abnormal eyes than homozygous normal mice after injection of trypan blue into their mothers during gestation   (MGI Ref ID J:15549)

Raxey1/Raxey1

        ZRDCT/Ch-Raxey1
  • vision/eye phenotype
  • abnormal lens morphology
    • at the 23 to 27 somite stage, the lens placode is not as firmly adherent to the optic vesicle as in wild-type mice (C3H)   (MGI Ref ID J:7407)
    • abnormal lens development
      • at E10, the lens is improperly centered in the optic cup and lens invagination is abnormal   (MGI Ref ID J:5962)
      • as early as the 28 somite stage, a precocious, undersized lens cup forms unlike in wild-type mice   (MGI Ref ID J:7407)
      • mice exhibit a deficiency of sulfated glycosaminoglycans in the basal lamina of the optic vesicle at its interface with the lens cup during lens cup invagination   (MGI Ref ID J:7407)
    • small lens   (MGI Ref ID J:5962)
  • abnormal optic cup morphology
    • at E10, the lens is smaller than in wild-type mice and may be improperly centered in the optic cup   (MGI Ref ID J:5962)
    • at the 35 to 45 somite stage, mice exhibit small optic cups surrounded by mesenchyme cells with no associated lens rudiment   (MGI Ref ID J:7407)
  • anophthalmia   (MGI Ref ID J:229)
    • in 70% of mice bilaterally   (MGI Ref ID J:5962)
  • microphthalmia   (MGI Ref ID J:229)
    • in 30% of mice   (MGI Ref ID J:5962)
  • behavior/neurological phenotype
  • abnormal circadian phase
    • fewer mice than normal entrained to restricted wheel access   (MGI Ref ID J:109897)

Raxey1/Raxey1

        involves: ZRDCT/Ch
  • nervous system phenotype
  • abnormal optic nerve morphology
    • the subjacent optic axons is absent   (MGI Ref ID J:5891)
  • abnormal suprachiasmatic nucleus morphology
    • the nucleus suprachiasmaticus (SCN) of the mediolateral hypothalamus is abnormal due to absence of the eye and subjacent optic axons   (MGI Ref ID J:5891)
  • vision/eye phenotype
  • abnormal optic nerve morphology
    • the subjacent optic axons is absent   (MGI Ref ID J:5891)
  • anophthalmia   (MGI Ref ID J:5891)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Raxey1 related

Neurobiology Research
Circadian Rhythms

Sensorineural Research
Eye Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Raxey1
Allele Name eyeless 1
Allele Type Spontaneous
Common Name(s) An; ZRDCT-An; anophthalmia; anophthalmic; ey; ey1;
Strain of OriginZRDCT/Ch
Gene Symbol and Name Rax, retina and anterior neural fold homeobox
Chromosome 18
Gene Common Name(s) E130303K03Rik; MCOP3; RIKEN cDNA E130303K03 gene; RX; ey-1; ey1; eyeless 1;
Molecular Note A A to T transversion results in a Met to Leu amino acid substitution at position 10. The mutation affects a conserved AUG codon that functions as an alternative translation initiation site and consequently reduces the abundance of protein. The mutation does not destabilize mRNA. [MGI Ref ID J:71979]

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

BECK SL. 1963. The anophthalmic mutant of the mouse. I. Genetic contribution to the anophthalmic phenotype. J Hered 54:39-44. [PubMed: 13970081]  [MGI Ref ID J:13083]

Kaiserman-Abramof IR; Graybiel AM; Nauta WJ. 1980. The thalamic projection to cortical area 17 in a congenitally anophthalmic mouse strain. Neuroscience 5(1):41-52. [PubMed: 7366842]  [MGI Ref ID J:109961]

Laemle LK; Fugaro C; Bentley T. 1993. The geniculohypothalamic pathway in a congenitally anophthalmic mouse. Brain Res 618(2):352-7. [PubMed: 8374768]  [MGI Ref ID J:109920]

Laemle LK; Hori N; Strominger NL; Tan Y; Carpenter DO. 2002. Physiological and anatomical properties of the suprachiasmatic nucleus of an anophthalmic mouse. Brain Res 953(1-2):73-81. [PubMed: 12384240]  [MGI Ref ID J:79711]

Laemle LK; Ottenweller JE. 1998. Daily patterns of running wheel activity in male anophthalmic mice. Physiol Behav 64(2):165-71. [PubMed: 9662081]  [MGI Ref ID J:109901]

Laemle LK; Ottenweller JE. 1999. Nonphotic entrainment of activity and temperature rhythms in anophthalmic mice. Physiol Behav 66(3):461-71. [PubMed: 10357435]  [MGI Ref ID J:109897]

Laemle LK; Ottenweller JE; Fugaro C. 1995. Diurnal variations in vasoactive intestinal polypeptide-like immunoreactivity in the suprachiasmatic nucleus of congenitally anophthalmic mice. Brain Res 688(1-2):203-8. [PubMed: 8542309]  [MGI Ref ID J:109913]

Laemle LK; Puszkarczuk M; Feinberg RN. 1999. Apoptosis in early ocular morphogenesis in the mouse. Brain Res Dev Brain Res 112(1):129-33. [PubMed: 9974166]  [MGI Ref ID J:109900]

Laemle LK; Rusa R. 1992. VIP-like immunoreactivity in the suprachiasmatic nuclei of a mutant anophthalmic mouse. Brain Res 589(1):124-8. [PubMed: 1422812]  [MGI Ref ID J:2244]

Silver J. 1977. Abnormal development of the suprachiasmatic nuclei of the hypothalamus in a strain of genetically anophthalmic mice. J Comp Neurol 176(4):589-606. [PubMed: 925203]  [MGI Ref ID J:5891]

Silver J; Puck SM; Albert DM. 1984. Development and aging of the eye in mice with inherited optic nerve aplasia: histopathological studies. Exp Eye Res 38(3):257-66. [PubMed: 6723805]  [MGI Ref ID J:45440]

Stein JH; Gottschall J; Osgood RW; Ferris TF. 1975. Pathophysiology of a nephrotoxic model of acute renal failure. Kidney Int 8(1):27-41. [PubMed: 1160223]  [MGI Ref ID J:109966]

Tucker P; Laemle L; Munson A; Kanekar S; Oliver ER; Brown N; Schlecht H; Vetter M; Glaser T. 2001. The eyeless mouse mutation (ey1) removes an alternative start codon from the Rx/rax homeobox gene. Genesis 31(1):43-53. [PubMed: 11668677]  [MGI Ref ID J:71979]

Additional References

Raxey1 related

BARBER AN. 1957. The effects of maternal hypoxia on inheritance of recessive blindness in mice. Am J Ophthalmol 44(4, Part 2):94-102. [PubMed: 13469955]  [MGI Ref ID J:15549]

BECK SL. 1963. The anophthalmic mutant of the mouse. II. An association of anophthalmia and polydactyly. J Hered 54:79-83. [PubMed: 13970082]  [MGI Ref ID J:13084]

Beck SL. 1960. Anophthalmic Mouse News Lett 22:15.  [MGI Ref ID J:24727]

Beck SL. 1963. Frequencies of teratologies among homozygous normal mice compared with those heterozygous for anophthalmia. Nature 200:810-811. [PubMed: 14087038]  [MGI Ref ID J:13117]

Chabot N; Charbonneau V; Laramee ME; Tremblay R; Boire D; Bronchti G. 2008. Subcortical auditory input to the primary visual cortex in anophthalmic mice. Neurosci Lett 433(2):129-34. [PubMed: 18276073]  [MGI Ref ID J:136522]

Chabot N; Robert S; Tremblay R; Miceli D; Boire D; Bronchti G. 2007. Audition differently activates the visual system in neonatally enucleated mice compared with anophthalmic mutants. Eur J Neurosci 26(8):2334-48. [PubMed: 17953622]  [MGI Ref ID J:127264]

Charbonneau V; Laramee ME; Boucher V; Bronchti G; Boire D. 2012. Cortical and subcortical projections to primary visual cortex in anophthalmic, enucleated and sighted mice. Eur J Neurosci 36(7):2949-63. [PubMed: 22780435]  [MGI Ref ID J:207654]

Chase HB. 1944. Studies on an Anophthalmic Strain of Mice. IV. a Second Major Gene for Anophthalmia. Genetics 29(3):264-9. [PubMed: 17247120]  [MGI Ref ID J:229]

Cullen MJ; Kaiserman-Abramof IR. 1976. Cytological organization of the dorsal lateral geniculate nuclei in mutant anophthalmic and postnatally enucleated mice. J Neurocytol 5(4):407-24. [PubMed: 993820]  [MGI Ref ID J:27569]

Faradji-Prevautel H; Cespuglio R; Jouvet M. 1990. Circadian rest-activity rhythms in the anophthalmic, monocular and binocular ZRDCT/An mice. Retinal and serotoninergic (raphe) influences. Brain Res 526(2):207-16. [PubMed: 2147868]  [MGI Ref ID J:45313]

Harch C; Chase HB; Gonsalves NI. 1978. Studies on an anophthalmic strain of mice. VI. Lens and cup interaction. Dev Biol 63(2):352-7. [PubMed: 640224]  [MGI Ref ID J:5962]

Kaiserman-Abramof IR. 1983. Intrauterine enucleation of normal mice mimics a structural compensatory response in the geniculate of eyeless mutant mice. Brain Res 270(1):149-53. [PubMed: 6871708]  [MGI Ref ID J:45310]

Laemle LK; Ottenweller JE. 2001. The relationship between circadian rhythmicity and vasoactive intestinal polypeptide in the suprachiasmatic nucleus of congenitally anophthalmic mice. Brain Res 917(1):105-11. [PubMed: 11602234]  [MGI Ref ID J:110087]

Olavarria J; van Sluyters RC. 1984. Callosal connections of the posterior neocortex in normal-eyed, congenitally anophthalmic, and neonatally enucleated mice. J Comp Neurol 230(2):249-68. [PubMed: 6512020]  [MGI Ref ID J:45311]

Piche M; Robert S; Miceli D; Bronchti G. 2004. Environmental enrichment enhances auditory takeover of the occipital cortex in anophthalmic mice. Eur J Neurosci 20(12):3463-72. [PubMed: 15610179]  [MGI Ref ID J:101269]

Rhoades RW; Mooney RD; Fish SE. 1984. A comparison of visual callosal organization in normal, bilaterally enucleated and congenitally anophthalmic mice. Exp Brain Res 56(1):92-105. [PubMed: 6468571]  [MGI Ref ID J:45405]

Rhoades RW; Mooney RD; Fish SE. 1985. Subcortical projections of area 17 in the anophthalmic mouse. Brain Res 349(1-2):171-81. [PubMed: 3986586]  [MGI Ref ID J:45312]

Webster EH Jr; Silver AF; Gonsalves NI. 1984. The extracellular matrix between the optic vesicle and presumptive lens during lens morphogenesis in an anophthalmic strain of mice. Dev Biol 103(1):142-50. [PubMed: 6201404]  [MGI Ref ID J:7407]

Zwaan J; Silver J. 1983. Crystallin synthesis in the lens rudiment of a strain of mice with congenital anophthalmia. Exp Eye Res 36(4):551-7. [PubMed: 6343105]  [MGI Ref ID J:27145]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $1650.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $2145.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

General Supply Notes

  • View the complete collection of spontaneous mutants in the Mouse Mutant Resource.

Control Information

  Control
   005005 ZRDCT Rax+/ChUmdJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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