Description

Strain Information

Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Background Strain NOD Donor Strain B6.D2 H2 Haplotype g7   Donating Investigator James Thomas,   Vanderbilt University

Appearance
albino, pink-eyed
Related Genotype: A/? Tyr^c/Tyr^c

Development
Thomas and Hulbert received from the American Type Culture Collection (ATCC) the BALB/c IgG1 mouse anti-human insulin monoclonal antibody, mAb125 that was the source of the immunoglobulin kappa chain VJ sequence in this transgene. This VJ sequence was used in place of the endogenous variable sequence downstream of the Igk-C sequence in the construct previously used by Goodenow et al., to generate anti-hen egg lysozyme transgenes. This new construct encodes a light chain that, when co-expressed with a transgene encoding the heavy chain with the VDJ of mAB125, binds most mammalian insulins. This construct was microinjected into (B6 x D2)F1 oocytes. The transgenic founders were backcrossed to C57BL/6 more than 10 times before being backcrossed and maintained on NOD/Tac for 25 generations. In 2004, N25 mice were backcrossed to NOD/LtJ at The Jackson Laboratory, and are maintained hemizygote x wild type.

Control Information

	Control
	Noncarrier
	001976 NOD/ShiLtJ
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Igk-C

007595	B6;129-Igk-Ctm1(IGKC)Mnz/J
002400	C57BL/6-Igk-Ctm1Cgn/J

View Strains carrying other alleles of Igk-C     (2 strains)

Additional Web Information

Congenic Nomenclature
Genetic Quality Control Annual Report

Phenotype

Phenotype Information

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Diabetes and Obesity Research
Type 1 Diabetes (IDDM)
Type 1 Diabetes (IDDM) Analysis Strains (NOD Transgenics)

Research Tools
Diabetes and Obesity Research

Igk-C related

Immunology and Inflammation Research
Autoimmunity
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Genes & Alleles

Gene & Allele Information

Allele Symbol		Tg(Igk-C/Igk-V125)1Jwt
Allele Name		transgene insertion 1, James W Thomas
Allele Type		Transgenic (random, expressed)
Common Name(s)		VK125; VK125-Ck;
Mutation Made By		James Thomas,   Vanderbilt University
Strain of Origin		(C57BL/6 x DBA/2)F1
Expressed Gene		Igk-C, immunoglobulin kappa chain, constant region, mouse, laboratory
Expressed Gene		Igk-V, immunoglobulin kappa chain complex variable region, mouse, laboratory
Promoter		Igk-C, immunoglobulin kappa chain, constant region, mouse, laboratory
Molecular Note		The transgene encodes a light chain that, when co-expressed with a transgene encoding the heavy chain with the VDJ of mAB125, binds most mammalian insulins. The source of the immunoglobulin kappa chain VJ sequence in this transgene was the BALB/c IgG1 mouse anti-human insulin monoclonal antibody, mAb125 (from the American Type Culture Collection). [MGI Ref ID J:91865]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Rojas M; Hulbert C; Thomas JW. 2001. Anergy and not clonal ignorance determines the fate of B cells that recognize a physiological autoantigen. J Immunol 166(5):3194-200. [PubMed: 11207272]  [MGI Ref ID J:91866]

Additional References

Tg(Igk-C/Igk-V125)1Jwt related

Acevedo-Suarez CA; Hulbert C; Woodward EJ; Thomas JW. 2005. Uncoupling of anergy from developmental arrest in anti-insulin B cells supports the development of autoimmune diabetes. J Immunol 174(2):827-33. [PubMed: 15634904]  [MGI Ref ID J:129804]

Hulbert C; Riseili B; Rojas M; Thomas JW. 2001. B cell specificity contributes to the outcome of diabetes in nonobese diabetic mice. J Immunol 167(10):5535-8. [PubMed: 11698422]  [MGI Ref ID J:91865]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Type 1 Diabetes Repository collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	Noncarrier
	001976 NOD/ShiLtJ
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

      Purchasing Information
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Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.