Strain Name:

NOD.Cg-Tg(Igh-6/Igh-V125)2Jwt/JwtJ

Stock Number:

005019

Availability:

Repository-Cryopreserved

Description

Strain Information

Type Congenic; Mutant Strain; Transgenic;
Additional information on Genetically Engineered Mutant Mice.
Specieslaboratory mouse
Background Strain NOD
Donor Strain B6.D2
H2 Haplotypeg7
 
Donating Investigator James Thomas,   Vanderbilt University

Appearance
albino, pink-eyed
Related Genotype: A/? Tyrc/Tyrc

Development
Thomas and Hulbert received from the American Type Culture Collection (ATCC) the BALB/c IgG1 mouse anti-human insulin monoclonal antibody, mAb125 that was the source of the immunoglobulin heavy chain VDJ sequence in this transgene. This VDJ sequence was used in place of the endogenous variable sequence downstream of the Igh-6 sequence in the construct previously used by Goodenow et al., to generate anti-hen egg lysozyme transgenes. This new construct encodes an IgMa constant region with the variable specificity from the mAb125 which, when co-expressed with a transgene encoding the light chain with the VJ of mAB125 (stock# 5018), binds insulin. This construct was microinjected into (B6 x D2)F1 oocytes. The transgenic founders were backcrossed to C57BL/6 more than 10 times before being backcrossed and maintained on NOD/Tac for more that 20 generations. In 2004, N25 mice were backcrossed to NOD/LtJ at The Jackson Laboratory, and are maintained hemizygote x wild type.

Control Information

  Control
   Noncarrier
   001976 NOD/ShiLtJ
   The double transgenic produced by crossing stock# 5020 with stock# 5018 serves as a control to the double transgenic which results from crossing stock#5018 with stock# 5019.
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of Igh-6     (12 strains)

View Strains carrying other alleles of Igh-V     (3 strains)

Additional Web Information

Congenic Nomenclature
Genetic Quality Control Annual Report

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Diabetes and Obesity Research
Type 1 Diabetes (IDDM)
Type 1 Diabetes (IDDM) Analysis Strains (NOD Transgenics)

Research Tools
Diabetes and Obesity Research

Igh-6 related

Immunology and Inflammation Research
Immunodeficiency (B cell deficiency)

Research Tools
Cancer Research (B cell deficiency)
Immunology and Inflammation Research (B cell deficiency)

Genes & Alleles

Gene & Allele Information

Allele Symbol Tg(Igh-6/Igh-V125)2Jwt
Allele Name transgene insertion 2, James W Thomas
Allele Type Transgenic (random, expressed)
Common Name(s) 125HC; 125VH; VH Tg125; VH125; VH125Tg;
Mutation Made By James Thomas,   Vanderbilt University
Strain of Origin(C57BL/6 x DBA/2)F1
Expressed Gene Igh-V, immunoglobulin heavy chain variable region, mouse, laboratory
Expressed Gene Igh-6, immunoglobulin heavy chain 6 (heavy chain of IgM), mouse, laboratory
Promoter Igh-6, immunoglobulin heavy chain 6 (heavy chain of IgM), mouse, laboratory
Molecular Note This construct encodes an IgMa constant region with the variable specificity from BALB/c IgG1 mouse anti-human insulin monoclonal antibody, mAb125 that was the source of the immunoglobulin heavy chain VDJ sequence in this transgene. The resulting antibody binds insulin when co-expressed with a transgene encoding the light chain with the VJ of mAB125. [MGI Ref ID J:91866]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Rojas M; Hulbert C; Thomas JW. 2001. Anergy and not clonal ignorance determines the fate of B cells that recognize a physiological autoantigen. J Immunol 166(5):3194-200. [PubMed: 11207272]  [MGI Ref ID J:91866]

Additional References

Tg(Igh-6/Igh-V125)2Jwt related

Acevedo-Suarez CA; Hulbert C; Woodward EJ; Thomas JW. 2005. Uncoupling of anergy from developmental arrest in anti-insulin B cells supports the development of autoimmune diabetes. J Immunol 174(2):827-33. [PubMed: 15634904]  [MGI Ref ID J:129804]

Hulbert C; Riseili B; Rojas M; Thomas JW. 2001. B cell specificity contributes to the outcome of diabetes in nonobese diabetic mice. J Immunol 167(10):5535-8. [PubMed: 11698422]  [MGI Ref ID J:91865]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $1900.00
*Price(s) in US dollars ($)

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $2470.00
*Price(s) in US dollars ($)

Additional Supply Details

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery - Standard.
    The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

  • This strain is included in the Type 1 Diabetes Repository collection.
  • Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

  Control
   Noncarrier
   001976 NOD/ShiLtJ
   The double transgenic produced by crossing stock# 5020 with stock# 5018 serves as a control to the double transgenic which results from crossing stock#5018 with stock# 5019.
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions


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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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Fax: 207.288.6150
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