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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Background Strain NOD Donor Strain NOD/MrkTacfBR H2 Haplotype g7 Generation N7F1+N1F1+N1p (15-MAY-05)
Generation DefinitionsDonating Investigator James Thomas, Vanderbilt University Appearance
albino, pink-eyed
Related Genotype: A/A Tyrc/TyrcDescription
The double transgenic produced by crossing this stock with Stock No. 005018 serves as a control to the double transgenic which results from crossing Stock No. 005018 with Stock No. 005019.Development
Thomas and Hulbert received the BALB/c IgG1 mouse anti-human insulin monoclonal antibody, mAb125 from the American Type Culture Collection (ATCC). This mab cross-reacts to mouse insulin. It was found that the variable region of the heavy chain of mAb125 has some sequence differences from the germ line sequence that generated this antibody sequence, referred to as VH-IX-281. When residues 49 and 56 of the variable region of the heavy chain of mAb125 are mutated back to the residues found in the germ line sequence, the resulting antibody no longer binds insulin with high affinity. The construct for Tg(Igh-6/Igh-V281)3Jwt has these germ line reversions (from G to D at residue 49 and from E to V at residue 56). This VDJ sequence, with these two residue changes, was cloned in place of the endogenous variable sequence downstream of the Ighm sequence in the construct previously used by Goodenow et al., to generate anti-hen egg lysozyme transgenes. This new construct encodes an IgMa constant region with a variable region nearly identical to that of mAB125 but lacking the high affinity specificity for insulin. This construct was microinjected into NOD/Tac oocytes and the transgenic strain has been mated to NOD/Tac for 7 generations. In 2004, N7 mice were backcrossed to NOD/LtJ at The Jackson Laboratory, and are maintained hemizygote x wild type.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001976 NOD/ShiLtJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Igh-V
003395 CD1-Tg(Igh-HOX11)11Idd/J 005019 NOD.Cg-Tg(Igh-6/Igh-V125)2Jwt/JwtJ 005309 NODCaj.Cg-Ighmtm1Cgn Tg(Igh-VB1-8/Igh-6)2Mjsk/FswJ 005306 NODCaj.Cg-Ighmtm1Cgn Tg(Igh-VB1-8/Igh-6m)1Mjsk/FswJ View Strains carrying other alleles of Igh-V (4 strains)
Additional Web Information
Genetic Quality Control Annual Report
Phenotype
Phenotype Information
assigned by genotype
Tg(Igh-6/Igh-V281)3Jwt/0
NOD.B6-Tg(Igh-6/Igh-V281)3Jwt
- endocrine/exocrine gland phenotype
- insulitis
- by 40 weeks, pancreata of transgenic mice show extensive infiltrates (MGI Ref ID J:91865)
- immune system phenotype
- decreased susceptibility to autoimmune diabetes
- transgenic mice are protected from developing diabetes; 16% develop diabetes by 40 weeks (MGI Ref ID J:91865)
- insulitis
- by 40 weeks, pancreata of transgenic mice show extensive infiltrates (MGI Ref ID J:91865)
This mouse can be used to support research in many areas including:
Diabetes and Obesity Research
Type 1 Diabetes (IDDM)
Type 1 Diabetes (IDDM) Analysis Strains
NOD Transgenics
Research Tools
Diabetes and Obesity Research
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Igh-6/Igh-V281)3Jwt | ||
|---|---|---|---|
| Allele Name | transgene insertion 3, James W Thomas | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | 281HC; H125.gl; VH281; VH281Tg; | ||
| Mutation Made By | James Thomas, Vanderbilt University | ||
| Strain of Origin | C57BL/6 | ||
| Expressed Gene | Igh-V, immunoglobulin heavy chain variable region, mouse, laboratory | ||
| Expressed Gene | Ighm, immunoglobulin heavy constant mu, mouse, laboratory | ||
| Promoter | Igh-V, immunoglobulin heavy chain variable region, mouse, laboratory | ||
| Promoter | Ighm, immunoglobulin heavy constant mu, mouse, laboratory | ||
| Molecular Note | The construct for Tg(Igh-6/Igh-V281)3Jwt has a germ line reversion encoding D at residue 49 (from G)and another encoding V (from E) at residue 56. The transgene encodes an IgMa constant region with a variable region nearly identical to that ofthe BALB/c IgG1 mouse anti-human insulin monoclonal antibody, mAb125, (from the American Type Culture Collection) but lacking the high affinity specificity for insulin. [MGI Ref ID J:91865] | ||
References
References provided by MGI
Selected Reference(s)
Rojas M; Hulbert C; Thomas JW. 2001. Anergy and not clonal ignorance determines the fate of B cells that recognize a physiological autoantigen. J Immunol 166(5):3194-200. [PubMed: 11207272] [MGI Ref ID J:91866]
Additional References
Tg(Igh-6/Igh-V281)3Jwt relatedHulbert C; Riseili B; Rojas M; Thomas JW. 2001. B cell specificity contributes to the outcome of diabetes in nonobese diabetic mice. J Immunol 167(10):5535-8. [PubMed: 11698422] [MGI Ref ID J:91865]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Embryos
Price (US dollars $) Frozen Embryo $1600.00 Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryopreserved Embryos
Available to most shipping destinations1
This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.
1 Shipments cannot be made to Australia due to Australian government import restrictions.
2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Embryos
Price (US dollars $) Frozen Embryo $2080.00 Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryopreserved Embryos
Available to most shipping destinations1
This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.
1 Shipments cannot be made to Australia due to Australian government import restrictions.
2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
General Supply Notes
- This strain is included in the Type 1 Diabetes Repository collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001976 NOD/ShiLtJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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Tel: 1-800-422-6423 or 1-207-288-5845
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
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