Strain Name:

B6.129S2-Il10rbtm1Agt/J

Stock Number:

005027

Order this mouse

Availability:

Repository- Live

These Il10rb knock-out mice may be useful in studies of the role of interleukin-10 in inflammatory diseases such as chronic colitis.

Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Mating SystemHomozygote x Homozygote         (Female x Male)   16-OCT-12
Specieslaboratory mouse
GenerationN?+F6 (12-DEC-13)
Generation Definitions
 
Donating Investigator Sherman Fong,   Genentech, Inc.

Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA) is detected by Northern blot analysis of ES cells. After 12 weeks of age, approximately 60% of mutant mice develop chronic colitis and increased numbers of splenocytes resulting in splenomegaly. Bone marrow-derived macrophages, splenocytes and peritoneal cells derived from homozygotes do not respond to IL-10. Mutant mice housed in non-SPF conditions do not breed well. This mutant mouse strain may be useful in studies of the role of interleukin-10 in inflammatory diseases such as chronic colitis.

Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exons 1 and 2, which encode the signal peptide and the first 39 amino acids. The construct was electroporated into 129S2/SvPas-derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The donating investigator reported that the resulting male chimeric animals were crossed to C57BL/6 female mice, and then backcrossed to C57BL/6J mice for 6 generations using a speed congenic protocol (see SNP note below).

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.

Control Information

  Control
   000664 C57BL/6J C57BL/6J mice (stock number 000664) may be used as controls.
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Hepatitis B Virus, Susceptibility to   (IL10RB)
Inflammatory Bowel Disease 25, Autosomal Recessive; IBD25   (IL10RB)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Il10rbtm1Agt/Il10rbtm1Agt

        involves: 129S2/SvPas * C57BL/6
  • immune system phenotype
  • abnormal granulocyte differentiation
    • increased granulocytopoiesis in sternal bone marrow   (MGI Ref ID J:45917)
  • abnormal lymph node medullary cord morphology
    • increased number of plasma cells in the medullary cords of mesenteric lymph nodes   (MGI Ref ID J:45917)
  • abnormal mesenteric lymph node morphology
    • mesenteric lymphadenopathy   (MGI Ref ID J:45917)
  • abnormal spleen morphology   (MGI Ref ID J:45917)
    • increased spleen weight
      • 4 fold increase in the spleen/body weight ratio   (MGI Ref ID J:45917)
    • spleen hyperplasia
      • increased numbers of splenocytes   (MGI Ref ID J:45917)
  • increased interferon-gamma secretion
    • there is an increase in the percentage of CD8 T cells producing IFN-gamma compared to wild-type mice   (MGI Ref ID J:134156)
  • increased interleukin-17 secretion
    • there is an increased percentage of CD4 T cells producing IL-17 in the mesenteric lymph nodes and the lamina propria of 4-5 week old mice   (MGI Ref ID J:134156)
  • increased leukocyte cell number
    • peripheral leukocytosis involving neutrophiles and monocytes   (MGI Ref ID J:45917)
  • large intestinal inflammation
    • mixed inflammatory cell mucosal inflammation in the large intestine   (MGI Ref ID J:45917)
    • predominantly involves macrophage, neutrophiles and T lymphocytes   (MGI Ref ID J:45917)
    • colitis
      • colitis is observed in mice 3-4 months of age with ulcerations found near the iliel-cecal junction, the ascending-to-descending colon transition and the anal-rectal junction   (MGI Ref ID J:134156)
  • hematopoietic system phenotype
  • abnormal granulocyte differentiation
    • increased granulocytopoiesis in sternal bone marrow   (MGI Ref ID J:45917)
  • abnormal spleen morphology   (MGI Ref ID J:45917)
    • increased spleen weight
      • 4 fold increase in the spleen/body weight ratio   (MGI Ref ID J:45917)
    • spleen hyperplasia
      • increased numbers of splenocytes   (MGI Ref ID J:45917)
  • decreased hemoglobin content
    • decreased peripheral hemoglobin   (MGI Ref ID J:45917)
  • extramedullary hematopoiesis
    • increased in the red pulp of the spleen   (MGI Ref ID J:45917)
    • involves erythroid, myeloid, and megakaryocytic lineages   (MGI Ref ID J:45917)
  • increased leukocyte cell number
    • peripheral leukocytosis involving neutrophiles and monocytes   (MGI Ref ID J:45917)
  • digestive/alimentary phenotype
  • abnormal crypts of Lieberkuhn morphology
    • occasional hyperplasia of crypt epithelium   (MGI Ref ID J:45917)
  • abnormal large intestine morphology
    • thickened and narrowed   (MGI Ref ID J:45917)
  • large intestinal inflammation
    • mixed inflammatory cell mucosal inflammation in the large intestine   (MGI Ref ID J:45917)
    • predominantly involves macrophage, neutrophiles and T lymphocytes   (MGI Ref ID J:45917)
    • colitis
      • colitis is observed in mice 3-4 months of age with ulcerations found near the iliel-cecal junction, the ascending-to-descending colon transition and the anal-rectal junction   (MGI Ref ID J:134156)
  • endocrine/exocrine gland phenotype
  • abnormal crypts of Lieberkuhn morphology
    • occasional hyperplasia of crypt epithelium   (MGI Ref ID J:45917)
  • cellular phenotype
  • abnormal granulocyte differentiation
    • increased granulocytopoiesis in sternal bone marrow   (MGI Ref ID J:45917)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Il10rbtm1Agt related

Immunology, Inflammation and Autoimmunity Research
Inflammation
      Inflammatory bowel disease
Lymphoid Tissue Defects

Internal/Organ Research
Spleen Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Il10rbtm1Agt
Allele Name Targeted mutation 1, Michael Aguet
Allele Type Targeted (knock-out)
Common Name(s) CRFB4-; IL-10-; IL-10R2-;
Mutation Made By Sherman Fong,   Genentech, Inc.
Strain of Origin129S2/SvPas
ES Cell Line NameD3
ES Cell Line Strain129S2/SvPas
Gene Symbol and Name Il10rb, interleukin 10 receptor, beta
Chromosome 16
Gene Common Name(s) 6620401D04Rik; AI528744; CDW210B; CRF2-4; CRFB4; Crfb4; D16H21S58; D21S58; D21S58h; D21S66; DNA segment, Chr 16, human D21S58; IL-10R2; Il10r2; RGD1560373; RIKEN cDNA 6620401D04 gene; cytokine receptor family 2, member 4; expressed sequence AI528744;
General Note Phenotypic Similarity to Human Syndrome: Inflammatory Bowel Disease (J:134156).
Molecular Note The first two exons, encoding 39 amino acids including the signal peptide, were replaced with a neomycin cassette. Northern blot analysis of homozygous mutant ES cells showed an absence of transcript. [MGI Ref ID J:45917]

Genotyping

Genotyping Information

Genotyping Protocols

Generic Neo Melt Curve Analysis, Melt Curve Analysis
Il10rbtm1Agt,

MELT


Il10rbtm1Agt, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Spencer SD; Di Marco F; Hooley J; Pitts-Meek S; Bauer M; Ryan AM; Sordat B; Gibbs VC; Aguet M. 1998. The orphan receptor CRF2-4 is an essential subunit of the interleukin 10 receptor. J Exp Med 187(4):571-8. [PubMed: 9463407]  [MGI Ref ID J:45917]

Additional References

Il10rbtm1Agt related

Brooks DG; Walsh KB; Elsaesser H; Oldstone MB. 2010. IL-10 directly suppresses CD4 but not CD8 T cell effector and memory responses following acute viral infection. Proc Natl Acad Sci U S A 107(7):3018-23. [PubMed: 20133700]  [MGI Ref ID J:157557]

Cai G; Nie X; Zhang W; Wu B; Lin J; Wang H; Jiang C; Shen Q. 2012. A regulatory role for IL-10 receptor signaling in development and B cell help of T follicular helper cells in mice. J Immunol 189(3):1294-302. [PubMed: 22753938]  [MGI Ref ID J:189772]

Cassani B; Villablanca EJ; Quintana FJ; Love PE; Lacy-Hulbert A; Blaner WS; Sparwasser T; Snapper SB; Weiner HL; Mora JR. 2011. Gut-tropic T cells that express integrin alpha4beta7 and CCR9 are required for induction of oral immune tolerance in mice. Gastroenterology 141(6):2109-18. [PubMed: 21925467]  [MGI Ref ID J:180414]

Gu Y; Yang J; Ouyang X; Liu W; Li H; Yang J; Bromberg J; Chen SH; Mayer L; Unkeless JC; Xiong H. 2008. Interleukin 10 suppresses Th17 cytokines secreted by macrophages and T cells. Eur J Immunol 38(7):1807-13. [PubMed: 18506885]  [MGI Ref ID J:137290]

Huang B; Pan PY; Li Q; Sato AI; Levy DE; Bromberg J; Divino CM; Chen SH. 2006. Gr-1+CD115+ immature myeloid suppressor cells mediate the development of tumor-induced T regulatory cells and T-cell anergy in tumor-bearing host. Cancer Res 66(2):1123-31. [PubMed: 16424049]  [MGI Ref ID J:106544]

Kang SS; Allen PM. 2005. Priming in the presence of IL-10 results in direct enhancement of CD8+ T cell primary responses and inhibition of secondary responses. J Immunol 174(9):5382-9. [PubMed: 15843536]  [MGI Ref ID J:98415]

Kang SS; Bloom SM; Norian LA; Geske MJ; Flavell RA; Stappenbeck TS; Allen PM. 2008. An antibiotic-responsive mouse model of fulminant ulcerative colitis. PLoS Med 5(3):e41. [PubMed: 18318596]  [MGI Ref ID J:134156]

Murai M; Turovskaya O; Kim G; Madan R; Karp CL; Cheroutre H; Kronenberg M. 2009. Interleukin 10 acts on regulatory T cells to maintain expression of the transcription factor Foxp3 and suppressive function in mice with colitis. Nat Immunol 10(11):1178-84. [PubMed: 19783988]  [MGI Ref ID J:157749]

Rivera A; Collins N; Stephan MT; Lipuma L; Leiner I; Pamer EG. 2009. Aberrant tissue localization of fungus-specific CD4+ T cells in IL-10-deficient mice. J Immunol 183(1):631-41. [PubMed: 19542472]  [MGI Ref ID J:149964]

Spach KM; Nashold FE; Dittel BN; Hayes CE. 2006. IL-10 signaling is essential for 1,25-dihydroxyvitamin D3-mediated inhibition of experimental autoimmune encephalomyelitis. J Immunol 177(9):6030-7. [PubMed: 17056528]  [MGI Ref ID J:140527]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX18

Colony Maintenance

Mating SystemHomozygote x Homozygote         (Female x Male)   16-OCT-12
Diet Information LabDiet® 5K20

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $195.00Female or MaleHomozygous for Il10rbtm1Agt  
Price per Pair (US dollars $)Pair Genotype
$390.00Homozygous for Il10rbtm1Agt x Homozygous for Il10rbtm1Agt  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $253.50Female or MaleHomozygous for Il10rbtm1Agt  
Price per Pair (US dollars $)Pair Genotype
$507.00Homozygous for Il10rbtm1Agt x Homozygous for Il10rbtm1Agt  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Control Information

  Control
   000664 C57BL/6J C57BL/6J mice (stock number 000664) may be used as controls.
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(6.6)