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- Description
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- Genes & alleles
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Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Hemizygote x Inbred (Female x Male) 01-MAR-06 Species laboratory mouse Generation N8+N10 (31-DEC-08) Donating Investigator Thomas Jessell, Columbia University/HHMI Description
These transgenic mice express Green Fluorescent Protein (GFP) under the direction of the mouse Mnx1 (also called Hlxb9) promoter. Mice homozygous for the transgenic insert are viable, fertile, do not display any gross behavioral abnormalities, but are smaller in size than wildtype littermates. Homozygous pups born to homozygous females have a high mortality rate. Transgenic mice display distinct expression of GFP in dendrites, axons and soma of spinal motor neurons, allowing identification, isolation and purification of spinal motor neurons by FACS. GFP expression mimics endogenous HLXB9 expression pattern. Fluorescence is detected in axons, dendrites and processes of spinal motor neurons at embryonic day 9.5 to postnatal day 10 aged mice. This mutant mouse strain represents a model that may be useful for purification and in vivo tracking of spinal motor neurons.Development
A transgenic construct containing a 9kb sequence of the 5' portion of the mouse Mnx1 gene, a GFP open reading frame, and bovine growth hormome polyadenylation site sequence was injected into fertilized B6CBAF1 mouse eggs.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (225 strains)
006570 STOCK Smn1tm1Msd Tg(Hlxb9-GFP)1Tmj Tg(SMN2)89Ahmb/J
006600 B6.129S1-Mnx1tm4(cre)Tmj/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
GFP relatedNeurobiology Research
Fluorescent protein expression in neural tissue
Research Tools
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers: neurons
Neurobiology Research
cell marker
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Hlxb9-GFP)1Tmj | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Thomas M Jessell | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | Gfp-HB9; Hb9-Gfp; Hb9::EGFP; Hb9:GFP-1B; Hlxb9:GFP; | ||
| Mutation Made By | Ivo Lieberam, Columbia University/HHMI | ||
| Strain of Origin | (C57BL/6 x CBA)F1 | ||
| Site of Expression | Dendrites, axons, and soma of spinal motor neurons display distinct expression of GFP. GFP expression mimics endogenous HLXB9 expression pattern. Fluorscence is detected in axons, dendrites, and processes of spinal motor neurons at embryonic day 9.5 to postnatal day 10 aged mice. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Mnx1, motor neuron and pancreas homeobox 1, mouse, laboratory | ||
| General Note | Homozygous transgenic mice on a genetic background that involves C57BL/6 and CBA are viable and fertile. They do not display any gross behavioral abnormalities, but are smaller in size than wildtype littermates. Homozygous pups born to homozygous femaleshave a high mortality rate. Transgenic mice display distinct expression of GFP in dendrites, axons, and soma of spinal motor neurons, allowing identification, isolation and purification of spinal motor neurons by FACS. GFP expression mimics endogenous HLXB9 expression pattern. Fluorescence is detected in axons, dendrites and processes of spinal motor neurons at embryonic day 9.5 to postnatal day 10 aged mice. | ||
| Molecular Note | The transgenic construct contains a 9kb sequence of the 5' portion of the mouse Hlxb9 gene, a Green Fluorescent Protein (GFP) open reading frame, and a bovine growth hormome polyadenylation site sequence. [MGI Ref ID J:88764] | ||
| Gene Symbol and Name | Tg(Hlxb9-GFP)1Tmj, transgene insertion 1, Thomas M Jessell | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Gfp-HB9; Hb9-Gfp; Hb9::EGFP; Hb9:GFP-1B; Hlxb9:GFP; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), Melt Curve Analysis
Fluorescent Proteins (Generic GFP), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Wichterle H; Lieberam I; Porter JA; Jessell TM. 2002. Directed differentiation of embryonic stem cells into motor neurons. Cell 110(3):385-97. [PubMed: 12176325] [MGI Ref ID J:88764]
Additional References
Tg(Hlxb9-GFP)1Tmj relatedDe Marco Garcia NV; Jessell TM. 2008. Early motor neuron pool identity and muscle nerve trajectory defined by postmitotic restrictions in Nkx6.1 activity. Neuron 57(2):217-31. [PubMed: 18215620] [MGI Ref ID J:132705]
Di Giorgio FP; Carrasco MA; Siao MC; Maniatis T; Eggan K. 2007. Non-cell autonomous effect of glia on motor neurons in an embryonic stem cell-based ALS model. Nat Neurosci 10(5):608-14. [PubMed: 17435754] [MGI Ref ID J:121861]
Franko AJ; Sharplin J. 1994. Development of fibrosis after lung irradiation in relation to inflammation and lung function in a mouse strain prone to fibrosis. Radiat Res 140(3):347-55. [PubMed: 7972687] [MGI Ref ID J:21834]
Franz CK; Rutishauser U; Rafuse VF. 2005. Polysialylated neural cell adhesion molecule is necessary for selective targeting of regenerating motor neurons. J Neurosci 25(8):2081-91. [PubMed: 15728848] [MGI Ref ID J:97409]
Hippenmeyer S; Huber RM; Ladle DR; Murphy K; Arber S. 2007. ETS transcription factor Erm controls subsynaptic gene expression in skeletal muscles. Neuron 55(5):726-40. [PubMed: 17785180] [MGI Ref ID J:126807]
Huber AB; Kania A; Tran TS; Gu C; De Marco Garcia N; Lieberam I; Johnson D; Jessell TM; Ginty DD; Kolodkin AL. 2005. Distinct roles for secreted semaphorin signaling in spinal motor axon guidance. Neuron 48(6):949-64. [PubMed: 16364899] [MGI Ref ID J:107594]
Liem KF Jr; He M; Ocbina PJ; Anderson KV. 2009. Mouse Kif7/Costal2 is a cilia-associated protein that regulates Sonic hedgehog signaling. Proc Natl Acad Sci U S A 106(32):13377-82. [PubMed: 19666503] [MGI Ref ID J:151965]
McGovern VL; Gavrilina TO; Beattie CE; Burghes AH. 2008. Embryonic motor axon development in the severe SMA mouse. Hum Mol Genet 17(18):2900-9. [PubMed: 18603534] [MGI Ref ID J:138317]
Mukouyama YS; Deneen B; Lukaszewicz A; Novitch BG; Wichterle H; Jessell TM; Anderson DJ. 2006. Olig2+ neuroepithelial motoneuron progenitors are not multipotent stem cells in vivo. Proc Natl Acad Sci U S A 103(5):1551-6. [PubMed: 16432183] [MGI Ref ID J:142333]
Mukouyama YS; Gerber HP; Ferrara N; Gu C; Anderson DJ. 2005. Peripheral nerve-derived VEGF promotes arterial differentiation via neuropilin 1-mediated positive feedback. Development 132(5):941-952. [PubMed: 15673567] [MGI Ref ID J:96924]
Nagai M; Re DB; Nagata T; Chalazonitis A; Jessell TM; Wichterle H; Przedborski S. 2007. Astrocytes expressing ALS-linked mutated SOD1 release factors selectively toxic to motor neurons. Nat Neurosci 10(5):615-22. [PubMed: 17435755] [MGI Ref ID J:121834]
Rousso DL; Gaber ZB; Wellik D; Morrisey EE; Novitch BG. 2008. Coordinated actions of the forkhead protein Foxp1 and Hox proteins in the columnar organization of spinal motor neurons. Neuron 59(2):226-40. [PubMed: 18667151] [MGI Ref ID J:141971]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry The resulting male transgenic mice were backcrossed for 8 generations on the C57BL6/J background. The strain is maintained as a hemizygote, due to high mortality of homozygous pups born to homozygous females. Mating System Hemizygote x Inbred (Female x Male) 01-MAR-06 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Hemizygous for Tg(Hlxb9-GFP)1Tmj
Pairs /Price (US dollars $) Pair Genotype $297.85 Hemizygous for Tg(Hlxb9-GFP)1Tmj x Noncarrier $297.85 Noncarrier x Hemizygous for Tg(Hlxb9-GFP)1Tmj $487.00 Hemizygous for Tg(Hlxb9-GFP)1Tmj x Hemizygous for Tg(Hlxb9-GFP)1Tmj
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Hemizygous for Tg(Hlxb9-GFP)1Tmj
Pairs /Price (US dollars $) Pair Genotype $387.30 Hemizygous for Tg(Hlxb9-GFP)1Tmj x Noncarrier $387.30 Noncarrier x Hemizygous for Tg(Hlxb9-GFP)1Tmj $633.10 Hemizygous for Tg(Hlxb9-GFP)1Tmj x Hemizygous for Tg(Hlxb9-GFP)1Tmj
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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