| |||||||||||||||||||
| Mice homozygous for the transgene display distinct expression of GFP in dendrites, axons and soma of spinal motor neurons at embryonic day 9.5 to postnatal day 10 aged mice. Expression mimics endogenous HLXB9 expression pattern. This mutant mouse strain represents a model that may be useful for purification and in vivo tracking of spinal motor neurons. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Hemizygote x Inbred (Female x Male) 01-MAR-06 Species laboratory mouse Generation N8+N14 (24-MAR-11)
Generation DefinitionsDonating Investigator Thomas M. Jessell, Columbia University/HHMI Description
These transgenic mice express Green Fluorescent Protein (GFP) under the direction of the mouse Mnx1 (also called Hlxb9) promoter. Mice homozygous for the transgenic insert are viable, fertile, do not display any gross behavioral abnormalities, but are smaller in size than wildtype littermates. Homozygous pups born to homozygous females have a high mortality rate. Transgenic mice display distinct expression of GFP in dendrites, axons and soma of spinal motor neurons, allowing identification, isolation and purification of spinal motor neurons by FACS. GFP expression mimics endogenous HLXB9 expression pattern. Fluorescence is detected in axons, dendrites and processes of spinal motor neurons at embryonic day 9.5 to postnatal day 10 aged mice. This mutant mouse strain represents a model that may be useful for purification and in vivo tracking of spinal motor neurons.Development
A transgenic construct containing a 9kb sequence of the 5' portion of the mouse Mnx1 gene, a GFP open reading frame, and bovine growth hormome polyadenylation site sequence was injected into fertilized B6CBAF1 mouse eggs.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (357 strains)
006570 STOCK Smn1tm1Msd Tg(Hlxb9-GFP)1Tmj Tg(SMN2)89Ahmb/J
006600 B6.129S1-Mnx1tm4(cre)Tmj/J 017953 B6;C-Tg(Mnx1-Gfra1)1Slp/J 010928 STOCK Mnx1tm1Spf/J 007022 STOCK Mnx1tm4(cre)Tmj Smn1tm1Msd Tg(SMN2*delta7)4299Ahmb Tg(SMN2)89Ahmb/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
GFP relatedNeurobiology Research
Fluorescent protein expression in neural tissue
Research Tools
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers
Tissue/Cell Markers: neurons
Neurobiology Research
cell marker
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Hlxb9-GFP)1Tmj | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Thomas M Jessell | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | Gfp-HB9; Hb9-Gfp; Hb9::EGFP; Hb9:GFP-1B; Hlxb9:GFP; | ||
| Mutation Made By | Ivo Lieberam, Columbia University/HHMI | ||
| Strain of Origin | (C57BL/6 x CBA)F1 | ||
| Site of Expression | Dendrites, axons, and soma of spinal motor neurons display distinct expression of GFP. GFP expression mimics endogenous HLXB9 expression pattern. Fluorscence is detected in axons, dendrites, and processes of spinal motor neurons at embryonic day 9.5 to postnatal day 10 aged mice. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Mnx1, motor neuron and pancreas homeobox 1, mouse, laboratory | ||
| Gene Symbol and Name | Tg(Hlxb9-GFP)1Tmj, transgene insertion 1, Thomas M Jessell | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Gfp-HB9; Hb9-Gfp; Hb9::EGFP; Hb9:GFP-1B; Hlxb9:GFP; | ||
| General Note | Homozygous transgenic mice on a genetic background that involves C57BL/6 and CBA are viable and fertile. They do not display any gross behavioral abnormalities, but are smaller in size than wildtype littermates. Homozygous pups born to homozygous femaleshave a high mortality rate. Transgenic mice display distinct expression of GFP in dendrites, axons, and soma of spinal motor neurons, allowing identification, isolation and purification of spinal motor neurons by FACS. GFP expression mimics endogenous HLXB9 expression pattern. Fluorescence is detected in axons, dendrites and processes of spinal motor neurons at embryonic day 9.5 to postnatal day 10 aged mice. | ||
| Molecular Note | The transgenic construct contains a 9kb sequence of the 5' portion of the mouse Hlxb9 gene, a Green Fluorescent Protein (GFP) open reading frame, and a bovine growth hormome polyadenylation site sequence. [MGI Ref ID J:88764] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), Melt Curve Analysis
Fluorescent Proteins (Generic GFP), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Wichterle H; Lieberam I; Porter JA; Jessell TM. 2002. Directed differentiation of embryonic stem cells into motor neurons. Cell 110(3):385-97. [PubMed: 12176325] [MGI Ref ID J:88764]
Additional References
Tg(Hlxb9-GFP)1Tmj relatedAebischer J; Cassina P; Otsmane B; Moumen A; Seilhean D; Meininger V; Barbeito L; Pettmann B; Raoul C. 2011. IFNgamma triggers a LIGHT-dependent selective death of motoneurons contributing to the non-cell-autonomous effects of mutant SOD1. Cell Death Differ 18(5):754-68. [PubMed: 21072055] [MGI Ref ID J:186976]
Agalliu D; Schieren I. 2009. Heterogeneity in the developmental potential of motor neuron progenitors revealed by clonal analysis of single cells in vitro. Neural Dev 4:2. [PubMed: 19123929] [MGI Ref ID J:160734]
Ashrafi S; Lalancette-Hebert M; Friese A; Sigrist M; Arber S; Shneider NA; Kaltschmidt JA. 2012. Wnt7A Identifies Embryonic gamma-Motor Neurons and Reveals Early Postnatal Dependence of gamma-Motor Neurons on a Muscle Spindle-Derived Signal. J Neurosci 32(25):8725-8731. [PubMed: 22723712] [MGI Ref ID J:185656]
Betley JN; Wright CV; Kawaguchi Y; Erdelyi F; Szabo G; Jessell TM; Kaltschmidt JA. 2009. Stringent specificity in the construction of a GABAergic presynaptic inhibitory circuit. Cell 139(1):161-74. [PubMed: 19804761] [MGI Ref ID J:157313]
Bos R; Sadlaoud K; Boulenguez P; Buttigieg D; Liabeuf S; Brocard C; Haase G; Bras H; Vinay L. 2013. Activation of 5-HT2A receptors upregulates the function of the neuronal K-Cl cotransporter KCC2. Proc Natl Acad Sci U S A 110(1):348-53. [PubMed: 23248270] [MGI Ref ID J:192530]
Chang Q; Martin LJ. 2011. Glycine receptor channels in spinal motoneurons are abnormal in a transgenic mouse model of amyotrophic lateral sclerosis. J Neurosci 31(8):2815-27. [PubMed: 21414903] [MGI Ref ID J:180922]
De Marco Garcia NV; Jessell TM. 2008. Early motor neuron pool identity and muscle nerve trajectory defined by postmitotic restrictions in Nkx6.1 activity. Neuron 57(2):217-31. [PubMed: 18215620] [MGI Ref ID J:132705]
Demireva EY; Shapiro LS; Jessell TM; Zampieri N. 2011. Motor neuron position and topographic order imposed by beta- and gamma-catenin activities. Cell 147(3):641-52. [PubMed: 22036570] [MGI Ref ID J:178688]
Di Giorgio FP; Carrasco MA; Siao MC; Maniatis T; Eggan K. 2007. Non-cell autonomous effect of glia on motor neurons in an embryonic stem cell-based ALS model. Nat Neurosci 10(5):608-14. [PubMed: 17435754] [MGI Ref ID J:121861]
Dudanova I; Gatto G; Klein R. 2010. GDNF acts as a chemoattractant to support ephrinA-induced repulsion of limb motor axons. Curr Biol 20(23):2150-6. [PubMed: 21109439] [MGI Ref ID J:171859]
Dudanova I; Kao TJ; Herrmann JE; Zheng B; Kania A; Klein R. 2012. Genetic evidence for a contribution of EphA:ephrinA reverse signaling to motor axon guidance. J Neurosci 32(15):5209-15. [PubMed: 22496566] [MGI Ref ID J:184451]
Franko AJ; Sharplin J. 1994. Development of fibrosis after lung irradiation in relation to inflammation and lung function in a mouse strain prone to fibrosis. Radiat Res 140(3):347-55. [PubMed: 7972687] [MGI Ref ID J:21834]
Franz CK; Rutishauser U; Rafuse VF. 2005. Polysialylated neural cell adhesion molecule is necessary for selective targeting of regenerating motor neurons. J Neurosci 25(8):2081-91. [PubMed: 15728848] [MGI Ref ID J:97409]
Hippenmeyer S; Huber RM; Ladle DR; Murphy K; Arber S. 2007. ETS transcription factor Erm controls subsynaptic gene expression in skeletal muscles. Neuron 55(5):726-40. [PubMed: 17785180] [MGI Ref ID J:126807]
Hirose M; Niewiadomski P; Tse G; Chi GC; Dong H; Lee A; Carpenter EM; Waschek JA. 2011. Pituitary adenylyl cyclase-activating peptide counteracts hedgehog-dependent motor neuron production in mouse embryonic stem cell cultures. J Neurosci Res 89(9):1363-74. [PubMed: 21674568] [MGI Ref ID J:175565]
Huber AB; Kania A; Tran TS; Gu C; De Marco Garcia N; Lieberam I; Johnson D; Jessell TM; Ginty DD; Kolodkin AL. 2005. Distinct roles for secreted semaphorin signaling in spinal motor axon guidance. Neuron 48(6):949-64. [PubMed: 16364899] [MGI Ref ID J:107594]
Huettl RE; Huber AB. 2011. Cranial nerve fasciculation and Schwann cell migration are impaired after loss of Npn-1. Dev Biol 359(2):230-41. [PubMed: 21925156] [MGI Ref ID J:178497]
Huettl RE; Soellner H; Bianchi E; Novitch BG; Huber AB. 2011. Npn-1 contributes to axon-axon interactions that differentially control sensory and motor innervation of the limb. PLoS Biol 9(2):e1001020. [PubMed: 21364975] [MGI Ref ID J:170679]
Liem KF Jr; He M; Ocbina PJ; Anderson KV. 2009. Mouse Kif7/Costal2 is a cilia-associated protein that regulates Sonic hedgehog signaling. Proc Natl Acad Sci U S A 106(32):13377-82. [PubMed: 19666503] [MGI Ref ID J:151965]
Luria V; Laufer E. 2007. Lateral motor column axons execute a ternary trajectory choice between limb and body tissues. Neural Dev 2:13. [PubMed: 17605791] [MGI Ref ID J:160874]
Mandai K; Guo T; St Hillaire C; Meabon JS; Kanning KC; Bothwell M; Ginty DD. 2009. LIG family receptor tyrosine kinase-associated proteins modulate growth factor signals during neural development. Neuron 63(5):614-27. [PubMed: 19755105] [MGI Ref ID J:154926]
Mavlyutov TA; Epstein ML; Liu P; Verbny YI; Ziskind-Conhaim L; Ruoho AE. 2012. Development of the sigma-1 receptor in C-terminals of motoneurons and colocalization with the N,N'-dimethyltryptamine forming enzyme, indole-N-methyl transferase. Neuroscience 206:60-8. [PubMed: 22265729] [MGI Ref ID J:184639]
McGovern VL; Gavrilina TO; Beattie CE; Burghes AH. 2008. Embryonic motor axon development in the severe SMA mouse. Hum Mol Genet 17(18):2900-9. [PubMed: 18603534] [MGI Ref ID J:138317]
Mukouyama YS; Deneen B; Lukaszewicz A; Novitch BG; Wichterle H; Jessell TM; Anderson DJ. 2006. Olig2+ neuroepithelial motoneuron progenitors are not multipotent stem cells in vivo. Proc Natl Acad Sci U S A 103(5):1551-6. [PubMed: 16432183] [MGI Ref ID J:142333]
Mukouyama YS; Gerber HP; Ferrara N; Gu C; Anderson DJ. 2005. Peripheral nerve-derived VEGF promotes arterial differentiation via neuropilin 1-mediated positive feedback. Development 132(5):941-952. [PubMed: 15673567] [MGI Ref ID J:96924]
Nagai M; Re DB; Nagata T; Chalazonitis A; Jessell TM; Wichterle H; Przedborski S. 2007. Astrocytes expressing ALS-linked mutated SOD1 release factors selectively toxic to motor neurons. Nat Neurosci 10(5):615-22. [PubMed: 17435755] [MGI Ref ID J:121834]
Rousso DL; Gaber ZB; Wellik D; Morrisey EE; Novitch BG. 2008. Coordinated actions of the forkhead protein Foxp1 and Hox proteins in the columnar organization of spinal motor neurons. Neuron 59(2):226-40. [PubMed: 18667151] [MGI Ref ID J:141971]
Ruggiu M; McGovern VL; Lotti F; Saieva L; Li DK; Kariya S; Monani UR; Burghes AH; Pellizzoni L. 2012. A role for SMN exon 7 splicing in the selective vulnerability of motor neurons in spinal muscular atrophy. Mol Cell Biol 32(1):126-38. [PubMed: 22037760] [MGI Ref ID J:183557]
Sabharwal P; Lee C; Park S; Rao M; Sockanathan S. 2011. GDE2 regulates subtype-specific motor neuron generation through inhibition of Notch signaling. Neuron 71(6):1058-70. [PubMed: 21943603] [MGI Ref ID J:178550]
Shneider NA; Brown MN; Smith CA; Pickel J; Alvarez FJ. 2009. Gamma motor neurons express distinct genetic markers at birth and require muscle spindle-derived GDNF for postnatal survival. Neural Dev 4:42. [PubMed: 19954518] [MGI Ref ID J:160727]
Son EY; Ichida JK; Wainger BJ; Toma JS; Rafuse VF; Woolf CJ; Eggan K. 2011. Conversion of mouse and human fibroblasts into functional spinal motor neurons. Cell Stem Cell 9(3):205-18. [PubMed: 21852222] [MGI Ref ID J:176048]
Wilson JM; Dombeck DA; Diaz-Rios M; Harris-Warrick RM; Brownstone RM. 2007. Two-photon calcium imaging of network activity in XFP-expressing neurons in the mouse. J Neurophysiol 97(4):3118-25. [PubMed: 17303810] [MGI Ref ID J:164053]
Zagoraiou L; Akay T; Martin JF; Brownstone RM; Jessell TM; Miles GB. 2009. A cluster of cholinergic premotor interneurons modulates mouse locomotor activity. Neuron 64(5):645-62. [PubMed: 20005822] [MGI Ref ID J:157468]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry The resulting male transgenic mice were backcrossed for 8 generations on the C57BL/6J background. The strain is maintained as a hemizygote, due to high mortality of homozygous pups born to homozygous females. Mating System Hemizygote x Inbred (Female x Male) 01-MAR-06 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Hemizygous for Tg(Hlxb9-GFP)1Tmj
Price per Pair (US dollars $) Pair Genotype $296.00 Hemizygous for Tg(Hlxb9-GFP)1Tmj x Noncarrier $296.00 Noncarrier x Hemizygous for Tg(Hlxb9-GFP)1Tmj $464.00 Hemizygous for Tg(Hlxb9-GFP)1Tmj x Hemizygous for Tg(Hlxb9-GFP)1Tmj Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Hemizygous for Tg(Hlxb9-GFP)1Tmj
Price per Pair (US dollars $) Pair Genotype $384.80 Hemizygous for Tg(Hlxb9-GFP)1Tmj x Noncarrier $384.80 Noncarrier x Hemizygous for Tg(Hlxb9-GFP)1Tmj $603.20 Hemizygous for Tg(Hlxb9-GFP)1Tmj x Hemizygous for Tg(Hlxb9-GFP)1Tmj Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
|
|
|
Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.