Strain Name: |
B6.Cg-Tg(Hlxb9-GFP)1Tmj/J |
|---|---|
Stock Number: |
005029 |
Availability: | Repository- Live |
Price and Supply Information | |
General Terms and Conditions |
| Genes & Alleles | GFP; Mnx1; Tg(Hlxb9-GFP)1Tmj; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Hemizygote x Inbred (Female x Male) Species laboratory mouse Donating Investigator Thomas Jessell, Columbia University/HHMI Generation N8+N9 (31-DEC-07) Strain Description
These transgenic mice express Green Fluorescent Protein (GFP) under the direction of the mouse Hlxb9 promoter. Mice homozygous for the transgenic insert are viable, fertile, do not display any gross behavioral abnormalities, but are smaller in size than wildtype littermates. Homozygous pups born to homozygous females have a high mortality rate. Transgenic mice display distinct expression of GFP in dendrites, axons and soma of spinal motor neurons, allowing identification, isolation and purification of spinal motor neurons by FACS. GFP expression mimics endogenous HLXB9 expression pattern. Fluorescence is detected in axons, dendrites and processes of spinal motor neurons at embryonic day 9.5 to postnatal day 10 aged mice. This mutant mouse strain represents a model that may be useful for purification and in vivo tracking of spinal motor neurons.Strain Development
A transgenic construct containing a 9kb sequence of the 5' portion of the mouse Hlxb9 gene, a GFP open reading frame, and bovine growth hormome polyadenylation site sequence was injected into fertilized B6CBAF1 mouse eggs.
Gene & Allele Details
| Allele Symbol | Tg(Hlxb9-GFP)1Tmj | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Thomas M Jessell | ||
| Common Name(s) | Gfp-HB9; Hb9-Gfp; Hb9:GFP-1B; Hlxb9:GFP; | ||
| Mutation Made By | Ivo Lieberam, Columbia University/HHMI | ||
| Strain of Origin | (C57BL/6 x CBA)F1 | ||
| Site of Expression | Dendrites, axons, and soma of spinal motor neurons display distinct expression of GFP. GFP expression mimics endogenous HLXB9 expression pattern. Fluorscence is detected in axons, dendrites, and processes of spinal motor neurons at embryonic day 9.5 to postnatal day 10 aged mice. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Mnx1, motor neuron and pancreas homeobox 1, mouse, laboratory | ||
| General Note | Homozygous transgenic mice on a genetic background that involves C57BL/6 and CBA are viable and fertile. They do not display any gross behavioral abnormalities, but are smaller in size than wildtype littermates. Homozygous pups born to homozygous femaleshave a high mortality rate. Transgenic mice display distinct expression of GFP in dendrites, axons, and soma of spinal motor neurons, allowing identification, isolation and purification of spinal motor neurons by FACS. GFP expression mimics endogenous HLXB9 expression pattern. Fluorescence is detected in axons, dendrites and processes of spinal motor neurons at embryonic day 9.5 to postnatal day 10 aged mice. | ||
| Molecular Note | The transgenic construct contains a 9kb sequence of the 5' portion of the mouse Hlxb9 gene, a Green Fluorescent Protein (GFP) open reading frame, and a bovine growth hormome polyadenylation site sequence. [J:88764] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Genotyping Protocols
Fluorescent Proteins (Generic GFP)
Colony Maintenance
| Breeding & Husbandry | The resulting male transgenic mice were backcrossed for 8 generations on the C57BL6/J background. The strain is maintained as a hemizygote, due to high mortality of homozygous pups born to homozygous females. |
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| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Fluorescent Protein Strains
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006570 STOCK Smn1tm1Msd Tg(Hlxb9-GFP)1Tmj Tg(SMN2)89Ahmb/J
006600 B6.129S1-Mnx1tm4(cre)Tmj/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Nomenclature Bulletin
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
GFP relatedNeurobiology Research
Fluorescent protein expression in neural tissue
Research Tools
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers: neurons)
Neurobiology Research (cell marker)
Research Tools
Fluorescent Proteins
References
Selected Reference(s)
Additional ReferencesWichterle H; Lieberam I; Porter JA; Jessell TM. 2002. Directed differentiation of embryonic stem cells into motor neurons. Cell 110(3):385-97. [PubMed: 12176325] [J:88764]
Price and Supply Information
| Strain Name: | B6.Cg-Tg(Hlxb9-GFP)1Tmj/J |
| Stock Number: | 005029 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
International
| Price(s) in US dollars ($) | Genotype(s) Provided | ||||
|---|---|---|---|---|---|
| Individual Mouse Price | $307.40 | Hemizygous for Tg(Hlxb9-GFP)1Tmj | |||
| Pair | $375.60 | Hemizygous for Tg(Hlxb9-GFP)1Tmj x Noncarrier | |||
| Pair | $375.30 | Noncarrier x Hemizygous for Tg(Hlxb9-GFP)1Tmj | |||
| Pair | $614.50 | Hemizygous for Tg(Hlxb9-GFP)1Tmj x Hemizygous for Tg(Hlxb9-GFP)1Tmj | |||
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-207-288-5845. Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE only available to non-profit entities.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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