Strain Name:

C57BL/6J-Ticam1Lps2/J

Stock Number:

005037

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Availability:

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Use Restrictions Apply, see Terms of Use
Macrophages in these mice fail to produce tumor necrosis factor (TNF) upon challenge from synthetic lipid A, endotoxin lipopolysaccharide (LPS), and dsRNA. These mice may be useful in studies of immunodeficiency and host response to bacterial endotoxins and viruses.

Description

Strain Information

Former Names C57BL/6J-AW046014Lps2/J    (Changed: 06-JUL-05 )
Type Chemically Induced Mutation; Mutant Strain;
Additional information on Genetically Engineered and Mutant Mice.
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Mating SystemHomozygote x Homozygote         (Female x Male)   01-MAR-06
Specieslaboratory mouse
Background Strain C57BL/6
GenerationF3+5N1F4 (31-DEC-08)
Generation Definitions
 
Donating Investigator Bruce Beutler,   University of Texas Southwestern Medical

Description
Mice that are homozygous for the mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This single base pair deletion mutation was induced by ENU mutagenesis. Unlike wildtype macrophages, macrophages derived from these animals fail to respond to synthetic lipid A, endotoxin lipopolysaccharide (LPS), and dsRNA with production of tumor necrosis factor (TNF). Macrophages are less susceptible to LPS-induced cytotoxicity. Nitrous oxide and type I interferon production in activated macrophages is impaired. Homozygotes exhibit increased susceptibility to mouse cytomegalovirus. Although homozygotes are resistant to challenges with LPS, the mice will become ill and some may die.

Development
This strain was developed using ENU mutagenesis of male C57BL/6 mice. The founding male (#9324) was selected from the F3 generation using a screening protocol for macrophage response to lipid A and endotoxin lopopolyscaccharide (LPS). The ENU treatment induced a single base pair deletion at codon 708.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Herpes Simplex Encephalitis, Susceptibility to, 4   (TICAM1)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Ticam1Lps2/Ticam1Lps2

        C57BL/6J-Ticam1Lps2
  • vision/eye phenotype
  • abnormal retinal progenitor cell morphology
    • more proliferating cells in the retina at 6 days of age   (MGI Ref ID J:141070)
    • neuronal differentiation of precursors   (MGI Ref ID J:141070)
  • nervous system phenotype
  • *normal* nervous system phenotype
    • no neurological defects after cerebral ischemia/reperfusion (as with controls)   (MGI Ref ID J:155613)
    • infarct size similar to controls   (MGI Ref ID J:155613)
  • digestive/alimentary phenotype
  • abnormal enterocyte physiology
    • flagellin stimulated primary intestinal epithelial cells display dramatically suppressed production of keratinocyte-derived cytokine, macrophage inflammatory protein 3 alpha, and IL-6   (MGI Ref ID J:167340)
  • decreased susceptibility to induced colitis
    • significantly improved survival from experimental colitis induced with dextran sodium sulfate and flagellin treatment   (MGI Ref ID J:167340)
    • much less histological damage to colon epithelium in induced colitis   (MGI Ref ID J:167340)
    • recovery from colitis is improved   (MGI Ref ID J:167340)
  • immune system phenotype
  • *normal* immune system phenotype
    • flagellin induction of IFN-beta production is unaffected   (MGI Ref ID J:131353)
    • abnormal circulating tumor necrosis factor level
      • ethanol containing diet fails to cause a significant increase in serum TNF as occurs in controls   (MGI Ref ID J:138963)
    • abnormal cytokine secretion
      • flagellin stimulated primary intestinal epithelial cells display dramatically suppressed production of keratinocyte-derived cytokine and macrophage inflammatory protein 3 alpha   (MGI Ref ID J:167340)
      • IL-1beta strongly induces keratinocyte-derived cytokine production   (MGI Ref ID J:167340)
      • decreased interleukin-6 secretion
        • flagellin stimulated primary intestinal epithelial cells display dramatically suppressed production   (MGI Ref ID J:167340)
    • decreased susceptibility to induced colitis
      • significantly improved survival from experimental colitis induced with dextran sodium sulfate and flagellin treatment   (MGI Ref ID J:167340)
      • much less histological damage to colon epithelium in induced colitis   (MGI Ref ID J:167340)
      • recovery from colitis is improved   (MGI Ref ID J:167340)
  • growth/size/body phenotype
  • abnormal postnatal growth/weight/body size
    • resistant to weight loss in experimental colitis   (MGI Ref ID J:167340)
  • liver/biliary system phenotype
  • decreased susceptibility to hepatic steatosis
    • fail to develop ethanol induced liver steatosis as occurs in controls   (MGI Ref ID J:138963)
  • homeostasis/metabolism phenotype
  • abnormal circulating tumor necrosis factor level
    • ethanol containing diet fails to cause a significant increase in serum TNF as occurs in controls   (MGI Ref ID J:138963)

Ticam1Lps2/Ticam1Lps2

        C57BL/6J-Ticam1Lps2/J
  • homeostasis/metabolism phenotype
  • decreased susceptibility to injury
    • following induction of necrotizing enterocolitis, mice exhibit preservation of the small intestinal mucosa compared with wild-type mice   (MGI Ref ID J:193668)

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Ticam1Lps2/Ticam1Lps2

        involves: C57BL/6
  • immune system phenotype
  • abnormal cytokine secretion
    • cytokine secretion induced by naked polyI:C is severely impaired by 5- to 10-fold   (MGI Ref ID J:110201)
    • however, secretion induced by transfected polyI:C is unaffected   (MGI Ref ID J:110201)
    • decreased tumor necrosis factor secretion
      • TNF production in response to LPS, synthetic lipid A, and dsRNA is decreased or abolished   (MGI Ref ID J:84896)
  • abnormal macrophage physiology
    • responses of macrophages to LPS are reduced, with decreased or abolished apoptosis, nitric oxide production, TNF production, and type I interferon production   (MGI Ref ID J:84896)
    • decreased macrophage apoptosis   (MGI Ref ID J:84896)
      • greatly reduced apoptosis of Tlr3 and Tlr4 stimulated macrophage   (MGI Ref ID J:92674)
    • decreased macrophage cytokine production
      • type I inferferon activity is undetected in culture medium of LPS- or dsRNA-activated macrophages   (MGI Ref ID J:84896)
      • no production of type I interferons in response to mouse cytomegalovirus   (MGI Ref ID J:84896)
    • decreased macrophage nitric oxide production
      • LPS-induced nitric oxide production by macrophages is abolished   (MGI Ref ID J:84896)
  • decreased circulating tumor necrosis factor level
    • 10,000 fold reduction in the TNF alpha response to lipopolysaccharide   (MGI Ref ID J:86521)
  • decreased susceptibility to bacterial infection
    • diminished response to lipopolysaccharide   (MGI Ref ID J:86521)
    • 10,000 fold reduction in the TNF alpha response to lipopolysaccharide   (MGI Ref ID J:86521)
    • considerable protection against Yersinia enterocolitica induced apoptosis of macrophage   (MGI Ref ID J:92674)
  • decreased susceptibility to endotoxin shock
    • though some mice become ill and die after intraperitoneal lipopolysaccharide (LPS) injection, the survival rate is significantly greater than that of wild-type   (MGI Ref ID J:84896)
  • increased susceptibility to viral infection
    • mice are more susceptible to cytomegalovirus infection, with increased lethality and higher viral titers than wild-type mice   (MGI Ref ID J:84896)
    • macrophage cultures infected with Vaccinia virus show higher viral titers than control macrophages   (MGI Ref ID J:84896)
  • homeostasis/metabolism phenotype
  • decreased circulating tumor necrosis factor level
    • 10,000 fold reduction in the TNF alpha response to lipopolysaccharide   (MGI Ref ID J:86521)
  • decreased macrophage nitric oxide production
    • LPS-induced nitric oxide production by macrophages is abolished   (MGI Ref ID J:84896)
  • cellular phenotype
  • decreased macrophage apoptosis   (MGI Ref ID J:84896)
    • greatly reduced apoptosis of Tlr3 and Tlr4 stimulated macrophage   (MGI Ref ID J:92674)
  • hematopoietic system phenotype
  • abnormal macrophage physiology
    • responses of macrophages to LPS are reduced, with decreased or abolished apoptosis, nitric oxide production, TNF production, and type I interferon production   (MGI Ref ID J:84896)
    • decreased macrophage apoptosis   (MGI Ref ID J:84896)
      • greatly reduced apoptosis of Tlr3 and Tlr4 stimulated macrophage   (MGI Ref ID J:92674)
    • decreased macrophage cytokine production
      • type I inferferon activity is undetected in culture medium of LPS- or dsRNA-activated macrophages   (MGI Ref ID J:84896)
      • no production of type I interferons in response to mouse cytomegalovirus   (MGI Ref ID J:84896)
    • decreased macrophage nitric oxide production
      • LPS-induced nitric oxide production by macrophages is abolished   (MGI Ref ID J:84896)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
Immunodeficiency

Research Tools
Immunology, Inflammation and Autoimmunity Research
      genes regulating susceptibility to infectious disease and endotoxin

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Ticam1Lps2
Allele Name lipopolysaccharide 2
Allele Type Chemically induced (ENU)
Common Name(s) Lps; Lps2; Lps2-; TRIF-; Ticam1-; TrifLps2; Trifm;
Mutation Made By Bruce Beutler,   University of Texas Southwestern Medical
Strain of OriginC57BL/6J
Gene Symbol and Name Ticam1, toll-like receptor adaptor molecule 1
Chromosome 17
Gene Common Name(s) AW046014; AW547018; IIAE6; MyD88-3; PRVTIRB; TICAM-1; TRIF; expressed sequence AW046014; expressed sequence AW547018;
Molecular Note ENU mutagenesis induced the deletion of a single guanine within codon 708, resulting in a frameshift mutation. The mutation was predicted to replace 24 C-terminal residues with 11 unrelated residues. The mutation was initially detected by screening macrophages for their competence to respond to LPS. [MGI Ref ID J:84896] [MGI Ref ID J:86521]

Genotyping

Genotyping Information

Genotyping Protocols

Ticam1Lps2 End Point, End Point Analysis


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Hoebe K; Du X; Georgel P; Janssen E; Tabeta K; Kim SO; Goode J; Lin P; Mann N; Mudd S; Crozat K; Sovath S; Han J; Beutler B. 2003. Identification of Lps2 as a key transducer of MyD88-independent TIR signalling. Nature 424(6950):743-8. [PubMed: 12872135]  [MGI Ref ID J:84896]

Additional References

Hoebe K; Janssen EM; Kim SO; Alexopoulou L; Flavell RA; Han J; Beutler B. 2003. Upregulation of costimulatory molecules induced by lipopolysaccharide and double-stranded RNA occurs by Trif-dependent and Trif-independent pathways. Nat Immunol 4(12):1223-9. [PubMed: 14625548]  [MGI Ref ID J:86617]

Ticam1Lps2 related

Aachoui Y; Leaf IA; Hagar JA; Fontana MF; Campos CG; Zak DE; Tan MH; Cotter PA; Vance RE; Aderem A; Miao EA. 2013. Caspase-11 protects against bacteria that escape the vacuole. Science 339(6122):975-8. [PubMed: 23348507]  [MGI Ref ID J:193387]

Albacker LA; Chaudhary V; Chang YJ; Kim HY; Chuang YT; Pichavant M; Dekruyff RH; Savage PB; Umetsu DT. 2013. Invariant natural killer T cells recognize a fungal glycosphingolipid that can induce airway hyperreactivity. Nat Med 19(10):1297-304. [PubMed: 23995283]  [MGI Ref ID J:202034]

Arnold CN; Pirie E; Dosenovic P; McInerney GM; Xia Y; Wang N; Li X; Siggs OM; Karlsson Hedestam GB; Beutler B. 2012. A forward genetic screen reveals roles for Nfkbid, Zeb1, and Ruvbl2 in humoral immunity. Proc Natl Acad Sci U S A :. [PubMed: 22761313]  [MGI Ref ID J:185495]

Ashtekar AR; Zhang P; Katz J; Deivanayagam CC; Rallabhandi P; Vogel SN; Michalek SM. 2008. TLR4-mediated activation of dendritic cells by the heat shock protein DnaK from Francisella tularensis. J Leukoc Biol 84(6):1434-46. [PubMed: 18708593]  [MGI Ref ID J:142302]

Baccarella A; Fontana MF; Chen EC; Kim CC. 2013. Toll-like receptor 7 mediates early innate immune responses to malaria. Infect Immun 81(12):4431-42. [PubMed: 24042114]  [MGI Ref ID J:202330]

Barrio L; Saez de Guinoa J; Carrasco YR. 2013. TLR4 signaling shapes B cell dynamics via MyD88-dependent pathways and Rac GTPases. J Immunol 191(7):3867-75. [PubMed: 23997213]  [MGI Ref ID J:205953]

Bhattacharyya S; Ratajczak CK; Vogt SK; Kelley C; Colonna M; Schreiber RD; Muglia LJ. 2010. TAK1 targeting by glucocorticoids determines JNK and IkappaB regulation in Toll-like receptor-stimulated macrophages. Blood 115(10):1921-31. [PubMed: 20065289]  [MGI Ref ID J:158841]

Blasius AL; Arnold CN; Georgel P; Rutschmann S; Xia Y; Lin P; Ross C; Li X; Smart NG; Beutler B. 2010. Slc15a4, AP-3, and Hermansky-Pudlak syndrome proteins are required for Toll-like receptor signaling in plasmacytoid dendritic cells. Proc Natl Acad Sci U S A 107(46):19973-8. [PubMed: 21045126]  [MGI Ref ID J:166600]

Bourgeois C; Majer O; Frohner IE; Lesiak-Markowicz I; Hildering KS; Glaser W; Stockinger S; Decker T; Akira S; Muller M; Kuchler K. 2011. Conventional Dendritic Cells Mount a Type I IFN Response against Candida spp. Requiring Novel Phagosomal TLR7-Mediated IFN-{beta} Signaling. J Immunol 186(5):3104-12. [PubMed: 21282509]  [MGI Ref ID J:169378]

Brandl K; Sun L; Neppl C; Siggs OM; Le Gall SM; Tomisato W; Li X; Du X; Maennel DN; Blobel CP; Beutler B. 2010. MyD88 signaling in nonhematopoietic cells protects mice against induced colitis by regulating specific EGF receptor ligands. Proc Natl Acad Sci U S A 107(46):19967-72. [PubMed: 21041656]  [MGI Ref ID J:166602]

Brandt EB; Gibson AM; Bass S; Rydyznski C; Khurana Hershey GK. 2013. Exacerbation of allergen-induced eczema in TLR4- and TRIF-deficient mice. J Immunol 191(7):3519-25. [PubMed: 23997219]  [MGI Ref ID J:205950]

Brzezinska AA; Johnson JL; Munafo DB; Ellis BA; Catz SD. 2009. Signalling mechanisms for Toll-like receptor-activated neutrophil exocytosis: key roles for interleukin-1-receptor-associated kinase-4 and phosphatidylinositol 3-kinase but not Toll/IL-1 receptor (TIR) domain-containing adaptor inducing IFN-beta (TRIF). Immunology 127(3):386-97. [PubMed: 19019092]  [MGI Ref ID J:155996]

Buchholz BM; Billiar TR; Bauer AJ. 2010. Dominant role of the MyD88-dependent signaling pathway in mediating early endotoxin-induced murine ileus. Am J Physiol Gastrointest Liver Physiol 299(2):G531-8. [PubMed: 20508155]  [MGI Ref ID J:163354]

Calderon Toledo C; Rogers TJ; Svensson M; Tati R; Fischer H; Svanborg C; Karpman D. 2008. Shiga toxin-mediated disease in MyD88-deficient mice infected with Escherichia coli O157:H7. Am J Pathol 173(5):1428-39. [PubMed: 18832584]  [MGI Ref ID J:143430]

Cekic C; Casella CR; Sag D; Antignano F; Kolb J; Suttles J; Hughes MR; Krystal G; Mitchell TC. 2011. MyD88-Dependent SHIP1 Regulates Proinflammatory Signaling Pathways in Dendritic Cells after Monophosphoryl Lipid A Stimulation of TLR4. J Immunol 186(7):3858-65. [PubMed: 21339365]  [MGI Ref ID J:170844]

Cervantes-Barragan L; Gil-Cruz C; Pastelin-Palacios R; Lang KS; Isibasi A; Ludewig B; Lopez-Macias C. 2009. TLR2 and TLR4 signaling shapes specific antibody responses to Salmonella typhi antigens. Eur J Immunol 39(1):126-35. [PubMed: 19130558]  [MGI Ref ID J:143726]

Cha HR; Ko HJ; Kim ED; Chang SY; Seo SU; Cuburu N; Ryu S; Kim S; Kweon MN. 2011. Mucosa-associated epithelial chemokine/CCL28 expression in the uterus attracts CCR10+ IgA plasma cells following mucosal vaccination via estrogen control. J Immunol 187(6):3044-52. [PubMed: 21832166]  [MGI Ref ID J:179255]

Chan YR; Liu JS; Pociask DA; Zheng M; Mietzner TA; Berger T; Mak TW; Clifton MC; Strong RK; Ray P; Kolls JK. 2009. Lipocalin 2 is required for pulmonary host defense against Klebsiella infection. J Immunol 182(8):4947-56. [PubMed: 19342674]  [MGI Ref ID J:147498]

Chang EY; Guo B; Doyle SE; Cheng G. 2007. Cutting edge: involvement of the type I IFN production and signaling pathway in lipopolysaccharide-induced IL-10 production. J Immunol 178(11):6705-9. [PubMed: 17513714]  [MGI Ref ID J:147854]

Chassin C; Goujon JM; Darche S; du Merle L; Bens M; Cluzeaud F; Werts C; Ogier-Denis E; Le Bouguenec C; Buzoni-Gatel D; Vandewalle A. 2006. Renal collecting duct epithelial cells react to pyelonephritis-associated Escherichia coli by activating distinct TLR4-dependent and -independent inflammatory pathways. J Immunol 177(7):4773-84. [PubMed: 16982918]  [MGI Ref ID J:139309]

Chen S; Sorrentino R; Shimada K; Bulut Y; Doherty TM; Crother TR; Arditi M. 2008. Chlamydia pneumoniae-induced foam cell formation requires MyD88-dependent and -independent signaling and is reciprocally modulated by liver X receptor activation. J Immunol 181(10):7186-93. [PubMed: 18981140]  [MGI Ref ID J:140935]

Chi H; Barry SP; Roth RJ; Wu JJ; Jones EA; Bennett AM; Flavell RA. 2006. Dynamic regulation of pro- and anti-inflammatory cytokines by MAPK phosphatase 1 (MKP-1) in innate immune responses. Proc Natl Acad Sci U S A 103(7):2274-9. [PubMed: 16461893]  [MGI Ref ID J:106068]

Choi YJ; Im E; Chung HK; Pothoulakis C; Rhee SH. 2010. TRIF mediates Toll-like receptor 5-induced signaling in intestinal epithelial cells. J Biol Chem 285(48):37570-8. [PubMed: 20855887]  [MGI Ref ID J:167340]

Clavarino G; Claudio N; Dalet A; Terawaki S; Couderc T; Chasson L; Ceppi M; Schmidt EK; Wenger T; Lecuit M; Gatti E; Pierre P. 2012. Protein phosphatase 1 subunit Ppp1r15a/GADD34 regulates cytokine production in polyinosinic:polycytidylic acid-stimulated dendritic cells. Proc Natl Acad Sci U S A 109(8):3006-11. [PubMed: 22315398]  [MGI Ref ID J:182014]

Copin R; De Baetselier P; Carlier Y; Letesson JJ; Muraille E. 2007. MyD88-dependent activation of B220-CD11b+LY-6C+ dendritic cells during Brucella melitensis infection. J Immunol 178(8):5182-91. [PubMed: 17404301]  [MGI Ref ID J:145275]

Copin R; Vitry MA; Hanot Mambres D; Machelart A; De Trez C; Vanderwinden JM; Magez S; Akira S; Ryffel B; Carlier Y; Letesson JJ; Muraille E. 2012. In situ microscopy analysis reveals local innate immune response developed around Brucella infected cells in resistant and susceptible mice. PLoS Pathog 8(3):e1002575. [PubMed: 22479178]  [MGI Ref ID J:195395]

Croker B; Crozat K; Berger M; Xia Y; Sovath S; Schaffer L; Eleftherianos I; Imler JL; Beutler B. 2007. ATP-sensitive potassium channels mediate survival during infection in mammals and insects. Nat Genet 39(12):1453-60. [PubMed: 18026101]  [MGI Ref ID J:126658]

Croker BA; Lawson BR; Berger M; Eidenschenk C; Blasius AL; Moresco EM; Sovath S; Cengia L; Shultz LD; Theofilopoulos AN; Pettersson S; Beutler BA. 2008. Inflammation and autoimmunity caused by a SHP1 mutation depend on IL-1, MyD88, and a microbial trigger. Proc Natl Acad Sci U S A 105(39):15028-33. [PubMed: 18806225]  [MGI Ref ID J:142845]

De Trez C; Pajak B; Brait M; Glaichenhaus N; Urbain J; Moser M; Lauvau G; Muraille E. 2005. TLR4 and Toll-IL-1 receptor domain-containing adapter-inducing IFN-beta, but not MyD88, regulate Escherichia coli-induced dendritic cell maturation and apoptosis in vivo. J Immunol 175(2):839-46. [PubMed: 16002681]  [MGI Ref ID J:100715]

Ding A; Yu H; Yang J; Shi S; Ehrt S. 2005. Induction of macrophage-derived SLPI by Mycobacterium tuberculosis depends on TLR2 but not MyD88. Immunology 116(3):381-9. [PubMed: 16236128]  [MGI Ref ID J:103462]

Duggan JM; You D; Cleaver JO; Larson DT; Garza RJ; Guzman Pruneda FA; Tuvim MJ; Zhang J; Dickey BF; Evans SE. 2011. Synergistic interactions of TLR2/6 and TLR9 induce a high level of resistance to lung infection in mice. J Immunol 186(10):5916-26. [PubMed: 21482737]  [MGI Ref ID J:173084]

Eigenbrod T; Franchi L; Munoz-Planillo R; Kirschning CJ; Freudenberg MA; Nunez G; Dalpke A. 2012. Bacterial RNA mediates activation of caspase-1 and IL-1beta release independently of TLRs 3, 7, 9 and TRIF but is dependent on UNC93B. J Immunol 189(1):328-36. [PubMed: 22634614]  [MGI Ref ID J:188954]

Embry CA; Franchi L; Nunez G; Mitchell TC. 2011. Mechanism of impaired NLRP3 inflammasome priming by monophosphoryl lipid A. Sci Signal 4(171):ra28. [PubMed: 21540455]  [MGI Ref ID J:185335]

Famakin BM; Mou Y; Ruetzler CA; Bembry J; Maric D; Hallenbeck JM. 2011. Disruption of downstream MyD88 or TRIF Toll-like receptor signaling does not protect against cerebral ischemia. Brain Res 1388:148-56. [PubMed: 21376021]  [MGI Ref ID J:172528]

Ferwerda G; Kullberg BJ; de Jong DJ; Girardin SE; Langenberg DM; van Crevel R; Ottenhoff TH; Van der Meer JW; Netea MG. 2007. Mycobacterium paratuberculosis is recognized by Toll-like receptors and NOD2. J Leukoc Biol 82(4):1011-8. [PubMed: 17652449]  [MGI Ref ID J:125203]

Foldi J; Chung AY; Xu H; Zhu J; Outtz HH; Kitajewski J; Li Y; Hu X; Ivashkiv LB. 2010. Autoamplification of Notch signaling in macrophages by TLR-induced and RBP-J-dependent induction of Jagged1. J Immunol 185(9):5023-31. [PubMed: 20870935]  [MGI Ref ID J:165202]

Fransen F; Stenger RM; Poelen MC; van Dijken HH; Kuipers B; Boog CJ; van Putten JP; van Els CA; van der Ley P. 2010. Differential effect of TLR2 and TLR4 on the immune response after immunization with a vaccine against Neisseria meningitidis or Bordetella pertussis. PLoS One 5(12):e15692. [PubMed: 21203418]  [MGI Ref ID J:168338]

Gaddis DE; Michalek SM; Katz J. 2011. TLR4 signaling via MyD88 and TRIF differentially shape the CD4+ T cell response to Porphyromonas gingivalis hemagglutinin B. J Immunol 186(10):5772-83. [PubMed: 21498664]  [MGI Ref ID J:173221]

Gais P; Tiedje C; Altmayr F; Gaestel M; Weighardt H; Holzmann B. 2010. TRIF signaling stimulates translation of TNF-alpha mRNA via prolonged activation of MK2. J Immunol 184(10):5842-8. [PubMed: 20375303]  [MGI Ref ID J:161009]

Gandhapudi SK; Chilton PM; Mitchell TC. 2013. TRIF is required for TLR4 mediated adjuvant effects on T cell clonal expansion. PLoS One 8(2):e56855. [PubMed: 23457630]  [MGI Ref ID J:197179]

Gasse P; Mary C; Guenon I; Noulin N; Charron S; Schnyder-Candrian S; Schnyder B; Akira S; Quesniaux VF; Lagente V; Ryffel B; Couillin I. 2007. IL-1R1/MyD88 signaling and the inflammasome are essential in pulmonary inflammation and fibrosis in mice. J Clin Invest 117(12):3786-99. [PubMed: 17992263]  [MGI Ref ID J:130776]

Gavin AL; Hoebe K; Duong B; Ota T; Martin C; Beutler B; Nemazee D. 2006. Adjuvant-enhanced antibody responses in the absence of toll-like receptor signaling. Science 314(5807):1936-8. [PubMed: 17185603]  [MGI Ref ID J:116772]

Georgel P; Jiang Z; Kunz S; Janssen E; Mols J; Hoebe K; Bahram S; Oldstone MB; Beutler B. 2007. Vesicular stomatitis virus glycoprotein G activates a specific antiviral Toll-like receptor 4-dependent pathway. Virology 362(2):304-13. [PubMed: 17292937]  [MGI Ref ID J:124488]

Geuking MB; Cahenzli J; Lawson MA; Ng DC; Slack E; Hapfelmeier S; McCoy KD; Macpherson AJ. 2011. Intestinal bacterial colonization induces mutualistic regulatory T cell responses. Immunity 34(5):794-806. [PubMed: 21596591]  [MGI Ref ID J:172118]

Gitlin L; Barchet W; Gilfillan S; Cella M; Beutler B; Flavell RA; Diamond MS; Colonna M. 2006. Essential role of mda-5 in type I IFN responses to polyriboinosinic:polyribocytidylic acid and encephalomyocarditis picornavirus. Proc Natl Acad Sci U S A 103(22):8459-64. [PubMed: 16714379]  [MGI Ref ID J:110201]

Grobner S; Schulz S; Soldanova I; Gunst DS; Waibel M; Wesselborg S; Borgmann S; Autenrieth IB. 2007. Absence of Toll-like receptor 4 signaling results in delayed Yersinia enterocolitica YopP-induced cell death of dendritic cells. Infect Immun 75(1):512-7. [PubMed: 17074859]  [MGI Ref ID J:116649]

Guilliams M; Crozat K; Henri S; Tamoutounour S; Grenot P; Devilard E; de Bovis B; Alexopoulou L; Dalod M; Malissen B. 2010. Skin-draining lymph nodes contain dermis-derived CD103(-) dendritic cells that constitutively produce retinoic acid and induce Foxp3(+) regulatory T cells. Blood 115(10):1958-68. [PubMed: 20068222]  [MGI Ref ID J:158129]

Guo B; Chang EY; Cheng G. 2008. The type I IFN induction pathway constrains Th17-mediated autoimmune inflammation in mice. J Clin Invest 118(5):1680-90. [PubMed: 18382764]  [MGI Ref ID J:136169]

Ha H; Lee JH; Kim HN; Kwak HB; Kim HM; Lee SE; Rhee JH; Kim HH; Lee ZH. 2008. Stimulation by TLR5 modulates osteoclast differentiation through STAT1/IFN-beta. J Immunol 180(3):1382-9. [PubMed: 18209032]  [MGI Ref ID J:131353]

Hassan F; Ren D; Zhang W; Merkel TJ; Gu XX. 2012. Moraxella catarrhalis activates murine macrophages through multiple toll like receptors and has reduced clearance in lungs from TLR4 mutant mice. PLoS One 7(5):e37610. [PubMed: 22662179]  [MGI Ref ID J:187305]

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Watts BA 3rd; George T; Good DW. 2013. Lumen LPS inhibits HCOFormula absorption in the medullary thick ascending limb through TLR4-PI3K-Akt-mTOR-dependent inhibition of basolateral Na+/H+ exchange. Am J Physiol Renal Physiol 305(4):F451-62. [PubMed: 23698118]  [MGI Ref ID J:200847]

Watts BA 3rd; George T; Sherwood ER; Good DW. 2011. Basolateral LPS inhibits NHE3 and HCOFormula absorption through TLR4/MyD88-dependent ERK activation in medullary thick ascending limb. Am J Physiol Cell Physiol 301(6):C1296-306. [PubMed: 21881005]  [MGI Ref ID J:178333]

Wei B; Su TT; Dalwadi H; Stephan RP; Fujiwara D; Huang TT; Brewer S; Chen L; Arditi M; Borneman J; Rawlings DJ; Braun J. 2008. Resident enteric microbiota and CD8(+) T cells shape the abundance of marginal zone B cells. Eur J Immunol 38(12):3411-3425. [PubMed: 19009526]  [MGI Ref ID J:141389]

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Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX11

Colony Maintenance

Breeding & HusbandryThis strain originated on a C57BL/6 background. Heterozygotes were intercrossed to generate homozygotes. SPF (Specific Pathogen Free) conditions should be implemented as these mice are immunodeficient.
Mating SystemHomozygote x Homozygote         (Female x Male)   01-MAR-06
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $195.00Female or MaleHomozygous for Ticam1Lps2  
Price per Pair (US dollars $)Pair Genotype
$390.00Homozygous for Ticam1Lps2 x Homozygous for Ticam1Lps2  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $253.50Female or MaleHomozygous for Ticam1Lps2  
Price per Pair (US dollars $)Pair Genotype
$507.00Homozygous for Ticam1Lps2 x Homozygous for Ticam1Lps2  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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