Description

Strain Information

Former Names C57BL/6J-KitW-sh/BsmJ    (Changed: 15-DEC-04 ) Type Congenic; Mutant Strain; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote         (Female x Male) Species laboratory mouse Background Strain C57BL/6 Donor Strain (C3H/HeH x 101/H) F1 Generation N15F1+F8 (18-DEC-07)

Appearance
white coat, black eyes
Related Genotype: a/a Kit ^W-sh/Kit^W-sh

Description
Kit mutations affect melanogenesis, hematopoiesis and gametogenesis. The sash mutation affects melanoblast survival. Melanoblast density is severely reduced in homozygotes by E12 (Cable et al., 1995). Homozygote are white with black eyes and some pigment around the ears. Heterozygotes are black with a white sash at the midline. The Kit^W-sh mutation affects Kit expression in a tissue specific manner. Kit expression is abolished in mast cells and mutant mice have a mast cell deficit (Tono et al., 1992, Berrozpe et al., 1999). However, young animals (<4 weeks) have been reported to have mast cells in skin (Yamakazi et al., 1994). Kit^W-sh mRNA is expressed normally in the cerebellum and is weakly expressed in testis and spleen (Tono et al., 1992). In contrast to the Kit^W and Kit^W-v mutations, Kit^W-sh germ cells and erythrocytes are not affected. Homozygtes have some hearing impairment, resulting from reduced numbers of melanocytes within the stria vascularis (Cable et al., 1994). The Kit^W-sh mutation is an inversion located proximal to the Kit locus and spanning a 2.8 Mb segment (Nagle et al., 1995, Berrozpe et al., 1999). This strain may be useful in studies related to melanogenesis and mast cell deficiency.

Development
The Kit^W-sh mutation arose spontaneously on a litter produced from a C3H/HeH x 101/H mating at the MRC Radiobiology Unit at Harwell (H) in the U. K. (Lyon et al., 1982). The founder female was identified by a white sash around her midsection. The strain was transferred to Dr. Karen Steel (Nihr), also at Harwell, to Dr. Rudolph Jaenisch (Jae) at MIT and to Dr. Peter Besmer (Bsm) at Sloan Kettering. Dr. Besmer donated the strain to The Jackson Laboratory in 2004. This strain was backcrossed at least ten times to C57BL/6 prior to the transfer to Dr. Besmer.

Control Information

	Control
	000664 C57BL/6J	(approximate)
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Kit

000599	B6 x B6CBCa Aw-J/A-T(5;13)264Ca KitW-v/J
006564	B6(C)-KitW-41J Gusbmps/BrkJ
000495	B6.C-H38c/By-KitW-56J/J
000560	B6.C-H7b/By KitW-50J/J
000122	B6.C3-KitW-44J/J
000991	B6.C58-KitW-57J/J
002283	B6.Cg-KitW-19H/EiJ
000133	B6.Cg-KitW-24J/J
000139	B6.Cg-KitW-25J/J
000164	B6.Cg-KitW/J
000194	B6.Cg-Lx KitW-v/J
000171	B6.D2-KitW-45J/J
001563	B6.D2-KitW-73J/J
001177	B6.LP-KitW-49J/J
000350	B6By.Cg-KitW-v MitfMi-wh T/J
000627	C3H/HeJ-KitW-x/J
000847	C3Sn.B6-KitW-39J/J
000166	C57BL/6J-KitW-17J/J
000167	C57BL/6J-KitW-18J/J
000169	C57BL/6J-KitW-20J/J
000117	C57BL/6J-KitW-34J/J
000128	C57BL/6J-KitW-35J/J
000134	C57BL/6J-KitW-37J/J
000062	C57BL/6J-KitW-39J/J
000121	C57BL/6J-KitW-40J/J
000119	C57BL/6J-KitW-41J/J
000127	C57BL/6J-KitW-42J/J
000129	C57BL/6J-KitW-43J/J
000990	C57BL/6J-KitW-55J/J
001179	C57BL/6J-KitW-62J/J
000049	C57BL/6J-KitW-v/J
000965	CBACa.C3-KitW-x/J
000092	FL/1Re-KitW/J
000993	NZB/BlNJ-KitW-59J/J
000692	WB/ReJ KitW/J
100410	WBB6F1/J-KitW/KitW-v/J

View Strains carrying other alleles of Kit     (36 strains)

Additional Web Information

Congenic Nomenclature
Genetic Quality Control Annual Report

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Kit^W-sh/Kit⁺

        involves: 101/H * C3H/HeH

• pigmentation phenotype
• variable body spotting (MGI Ref ID J:24351)
  • appears as a distinctive broad white sash
• skin/coat/nails phenotype
• variable body spotting (MGI Ref ID J:24351)
  • appears as a distinctive broad white sash

Kit^W-sh/Kit^W-sh

        involves: 101/H * C3H/HeH

• pigmentation phenotype
• abnormal coat color (MGI Ref ID J:6857)
  • the coat is white except for small patches on or near the ears and, infrequently, at the base of the tail
• absent coat pigmentation (MGI Ref ID J:24351)
  • classic black eyes and white coat
• skin/coat/nails phenotype
• abnormal coat color (MGI Ref ID J:6857)
  • the coat is white except for small patches on or near the ears and, infrequently, at the base of the tail
• absent coat pigmentation (MGI Ref ID J:24351)
  • classic black eyes and white coat
• hematopoietic system phenotype
• abnormal hematopoiesis (MGI Ref ID J:6857)
• abnormal erythrocyte cell number (MGI Ref ID J:6857)
• decreased erythrocyte cell number (MGI Ref ID J:6857)
  • on this background,lower than normal mean hematocrit values were found but did not manifest as an anemia

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Sensorineural Research
Vestibular and Hearing Defects

Kit^W-sh related

Dermatology Research
Color and White Spotting Defects

Immunology and Inflammation Research
Immunodeficiency (Mast Cell Deficiency)

Research Tools
Immunology and Inflammation Research (Mast Cell Deficiency)

Genes & Alleles

Gene & Allele Information

Allele Symbol		KitW-sh
Allele Name		sash
Allele Type		Spontaneous
Common Name(s)		W-sh; Wsh;
Strain of Origin		(C3H/HeH x 101/H)F1
Gene Symbol and Name		Kit, kit oncogene
Chromosome		5
Gene Common Name(s)		Bs; C-Kit; CD117; Dominant white spotting; Fdc; Gsfsco1; Gsfsco5; Gsfsow3; PBT; SCFR; SCO1; SCO5; SOW3; Ssm; Steel Factor Receptor; Tr-kit; W; belly-spot; dominant spotting; gsf spotted coat 1; gsf spotted coat 5; phenotype like Sl or W 3; spotted sterile male;
Molecular Note		The molecular mutation of this allele is an inversion located proximal to the Kit structural gene that disrupts 5' regulatory sequences. Transcripts were not detectable from this allele in cultured mast cells derived from homozygous mice. However, an analysis of embryonic expression revealed that ectopic expression of Kit occurred in homozygous mice and normal expression was ablated. [MGI Ref ID J:13166] [MGI Ref ID J:25082] [MGI Ref ID J:2535] [MGI Ref ID J:29686] [MGI Ref ID J:6857]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Berrozpe G; Timokhina I; Yukl S; Tajima Y; Ono M; Zelenetz AD; Besmer P. 1999. The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit. Blood 94(8):2658-66. [PubMed: 10515869]  [MGI Ref ID J:109894]

Cable J; Huszar D; Jaenisch R; Steel KP. 1994. Effects of mutations at the W locus (c-kit) on inner ear pigmentation and function in the mouse. Pigment Cell Res 7(1):17-32. [PubMed: 7521050]  [MGI Ref ID J:21178]

Cable J; Jackson IJ; Steel KP. 1995. Mutations at the W locus affect survival of neural crest-derived melanocytes in the mouse. Mech Dev 50(2-3):139-50. [PubMed: 7619726]  [MGI Ref ID J:24351]

Duttlinger R; Manova K; Berrozpe G; Chu TY; DeLeon V; Timokhina I; Chaganti RS; Zelenetz AD; Bachvarova RF; Besmer P. 1995. The Wsh and Ph mutations affect the c-kit expression profile: c-kit misexpression in embryogenesis impairs melanogenesis in Wsh and Ph mutant mice. Proc Natl Acad Sci U S A 92(9):3754-8. [PubMed: 7537375]  [MGI Ref ID J:25082]

Duttlinger R; Manova K; Chu TY; Gyssler C; Zelenetz AD; Bachvarova RF; Besmer P. 1993. W-sash affects positive and negative elements controlling c-kit expression: ectopic c-kit expression at sites of kit-ligand expression affects melanogenesis. Development 118(3):705-17. [PubMed: 7521281]  [MGI Ref ID J:13166]

Lyon MF; Glenister PH. 1982. A new allele sash (Wsh) at the W-locus and a spontaneous recessive lethal in mice. Genet Res 39(3):315-22. [PubMed: 7117838]  [MGI Ref ID J:6857]

Nagle DL; Kozak CA; Mano H; Chapman VM; Bucan M. 1995. Physical mapping of the Tec and Gabrb1 loci reveals that the Wsh mutation on mouse chromosome 5 is associated with an inversion. Hum Mol Genet 4(11):2073-9. [PubMed: 8589683]  [MGI Ref ID J:29686]

Tono T; Tsujimura T; Koshimizu U; Kasugai T; Adachi S; Isozaki K; Nishikawa S; Morimoto M; Nishimune Y; Nomura S; Kitamura Y.. 1992. c-kit Gene was not transcribed in cultured mast cells of mast cell-deficient Wsh/Wsh mice that have a normal number of erythrocytes and a normal c-kit coding region. Blood 80(6):1448-53. [PubMed: 1381627]  [MGI Ref ID J:2535]

Yamazaki M; Tsujimura T; Morii E; Isozaki K; Onoue H; Nomura S; Kitamura Y. 1994. C-kit gene is expressed by skin mast cells in embryos but not in puppies of Wsh/Wsh mice: age-dependent abolishment of c-kit gene expression. Blood 83(12):3509-16. [PubMed: 7515715]  [MGI Ref ID J:18760]

Additional References

Kit^W-sh related

Brown JM; Swindle EJ; Kushnir-Sukhov NM; Holian A; Metcalfe DD. 2007. Silica-directed mast cell activation is enhanced by scavenger receptors. Am J Respir Cell Mol Biol 36(1):43-52. [PubMed: 16902192]  [MGI Ref ID J:130522]

Byrne SN; Limon-Flores AY; Ullrich SE. 2008. Mast Cell Migration from the Skin to the Draining Lymph Nodes upon Ultraviolet Irradiation Represents a Key Step in the Induction of Immune Suppression. J Immunol 180(7):4648-55. [PubMed: 18354188]  [MGI Ref ID J:133390]

Di Nardo A; Yamasaki K; Dorschner RA; Lai Y; Gallo RL. 2008. Mast cell cathelicidin antimicrobial peptide prevents invasive group a streptococcus infection of the skin. J Immunol 180(11):7565-73. [PubMed: 18490758]  [MGI Ref ID J:136326]

Grimbaldeston MA; Chen CC; Piliponsky AM; Tsai M; Tam SY; Galli SJ. 2005. Mast cell-deficient W-sash c-kit mutant Kit W-sh/W-sh mice as a model for investigating mast cell biology in vivo. Am J Pathol 167(3):835-48. [PubMed: 16127161]  [MGI Ref ID J:101685]

Grimbaldeston MA; Nakae S; Kalesnikoff J; Tsai M; Galli SJ. 2007. Mast cell-derived interleukin 10 limits skin pathology in contact dermatitis and chronic irradiation with ultraviolet B. Nat Immunol 8(10):1095-104. [PubMed: 17767162]  [MGI Ref ID J:125267]

Hua X; Kovarova M; Chason KD; Nguyen M; Koller BH; Tilley SL. 2007. Enhanced mast cell activation in mice deficient in the A2b adenosine receptor. J Exp Med 204(1):117-28. [PubMed: 17200408]  [MGI Ref ID J:125297]

Iyer AS; August A. 2008. The Tec family kinase, IL-2-inducible T cell kinase, differentially controls mast cell responses. J Immunol 180(12):7869-77. [PubMed: 18523250]  [MGI Ref ID J:137249]

Jessup HK; Brewer AW; Omori M; Rickel EA; Budelsky AL; Yoon BR; Ziegler SF; Comeau MR. 2008. Intradermal administration of thymic stromal lymphopoietin induces a T cell- and eosinophil-dependent systemic Th2 inflammatory response. J Immunol 181(6):4311-9. [PubMed: 18768889]  [MGI Ref ID J:139079]

Kakurai M; Monteforte R; Suto H; Tsai M; Nakae S; Galli SJ. 2006. Mast cell-derived tumor necrosis factor can promote nerve fiber elongation in the skin during contact hypersensitivity in mice. Am J Pathol 169(5):1713-21. [PubMed: 17071594]  [MGI Ref ID J:114981]

Kitamura Y; Yamazaki M; Nomura S; Tsujimura T. 1993. Cell type specific and age specific suppression of c-kit gene expression in mutant mice of Wsh/Wsh genotype Exp Hematol 21(8):1175 (Abstr.).  [MGI Ref ID J:13326]

Lu LF; Lind EF; Gondek DC; Bennett KA; Gleeson MW; Pino-Lagos K; Scott ZA; Coyle AJ; Reed JL; Van Snick J; Strom TB; Zheng XX; Noelle RJ. 2006. Mast cells are essential intermediaries in regulatory T-cell tolerance. Nature 442(7106):997-1002. [PubMed: 16921386]  [MGI Ref ID J:112226]

Mallen-St Clair J; Pham CT; Villalta SA; Caughey GH; Wolters PJ. 2004. Mast cell dipeptidyl peptidase I mediates survival from sepsis. J Clin Invest 113(4):628-34. [PubMed: 14966572]  [MGI Ref ID J:88160]

Metz M; Piliponsky AM; Chen CC; Lammel V; Abrink M; Pejler G; Tsai M; Galli SJ. 2006. Mast cells can enhance resistance to snake and honeybee venoms. Science 313(5786):526-30. [PubMed: 16873664]  [MGI Ref ID J:110940]

Miyata M; Hatsushika K; Ando T; Shimokawa N; Ohnuma Y; Katoh R; Suto H; Ogawa H; Masuyama K; Nakao A. 2008. Mast cell regulation of epithelial TSLP expression plays an important role in the development of allergic rhinitis. Eur J Immunol 38(6):1487-92. [PubMed: 18461563]  [MGI Ref ID J:136211]

Nakae S; Suto H; Kakurai M; Sedgwick JD; Tsai M; Galli SJ. 2005. Mast cells enhance T cell activation: Importance of mast cell-derived TNF. Proc Natl Acad Sci U S A 102(18):6467-72. [PubMed: 15840716]  [MGI Ref ID J:98468]

Nocka K; Tan JC; Chiu E; Chu TY; Ray P; Traktman P; Besmer P. 1990. Molecular bases of dominant negative and loss of function mutations at the murine c-kit/white spotting locus: W37, Wv, W41 and W. EMBO J 9(6):1805-13. [PubMed: 1693331]  [MGI Ref ID J:10528]

Olivera A; Mizugishi K; Tikhonova A; Ciaccia L; Odom S; Proia RL; Rivera J. 2007. The sphingosine kinase-sphingosine-1-phosphate axis is a determinant of mast cell function and anaphylaxis. Immunity 26(3):287-97. [PubMed: 17346996]  [MGI Ref ID J:120074]

Reese TA; Liang HE; Tager AM; Luster AD; Van Rooijen N; Voehringer D; Locksley RM. 2007. Chitin induces accumulation in tissue of innate immune cells associated with allergy. Nature 447(7140):92-6. [PubMed: 17450126]  [MGI Ref ID J:122735]

Rudick CN; Bryce PJ; Guichelaar LA; Berry RE; Klumpp DJ. 2008. Mast cell-derived histamine mediates cystitis pain. PLoS ONE 3(5):e2096. [PubMed: 18461160]  [MGI Ref ID J:136218]

Silvers WK. 1979. The Coat Colors of Mice; A Model for Mammalian Gene Action and Interaction. In: The Coat Colors of Mice. Springer-Verlag, New York.  [MGI Ref ID J:78801]

Sinnamon MJ; Carter KJ; Sims LP; Lafleur B; Fingleton B; Matrisian LM. 2008. A protective role of mast cells in intestinal tumorigenesis. Carcinogenesis 29(4):880-6. [PubMed: 18258601]  [MGI Ref ID J:133499]

Soucek L; Lawlor ER; Soto D; Shchors K; Swigart LB; Evan GI. 2007. Mast cells are required for angiogenesis and macroscopic expansion of Myc-induced pancreatic islet tumors. Nat Med 13(10):1211-8. [PubMed: 17906636]  [MGI Ref ID J:130030]

Stephenson DA; Glenister PH; Hornby JE. 1985. Site of beige (bg) and leaden (ln) pigment gene expression determined by recombinant embryonic skin grafts and aggregation mouse chimaeras employing sash (Wsh) homozygotes. Genet Res 46(2):193-205. [PubMed: 3910518]  [MGI Ref ID J:8167]

Sun J; Sukhova GK; Wolters PJ; Yang M; Kitamoto S; Libby P; MacFarlane LA; Mallen-St Clair J; Shi GP. 2007. Mast cells promote atherosclerosis by releasing proinflammatory cytokines. Nat Med 13(6):719-24. [PubMed: 17546038]  [MGI Ref ID J:125116]

Sun J; Sukhova GK; Yang M; Wolters PJ; MacFarlane LA; Libby P; Sun C; Zhang Y; Liu J; Ennis TL; Knispel R; Xiong W; Thompson RW; Baxter BT; Shi GP. 2007. Mast cells modulate the pathogenesis of elastase-induced abdominal aortic aneurysms in mice. J Clin Invest 117(11):3359-68. [PubMed: 17932568]  [MGI Ref ID J:127400]

Suto H; Nakae S; Kakurai M; Sedgwick JD; Tsai M; Galli SJ. 2006. Mast cell-associated TNF promotes dendritic cell migration. J Immunol 176(7):4102-12. [PubMed: 16547246]  [MGI Ref ID J:129877]

Tsujimura Y; Obata K; Mukai K; Shindou H; Yoshida M; Nishikado H; Kawano Y; Minegishi Y; Shimizu T; Karasuyama H. 2008. Basophils play a pivotal role in immunoglobulin-G-mediated but not immunoglobulin-E-mediated systemic anaphylaxis. Immunity 28(4):581-9. [PubMed: 18342553]  [MGI Ref ID J:134463]

Wolters PJ; Mallen-St Clair J; Lewis CC; Villalta SA; Baluk P; Erle DJ; Caughey GH. 2005. Tissue-selective mast cell reconstitution and differential lung gene expression in mast cell-deficient Kit(W-sh)/Kit(W-sh) sash mice. Clin Exp Allergy 35(1):82-8. [PubMed: 15649271]  [MGI Ref ID J:109813]

Xu X; Zhang D; Zhang H; Wolters PJ; Killeen NP; Sullivan BM; Locksley RM; Lowell CA; Caughey GH. 2006. Neutrophil histamine contributes to inflammation in mycoplasma pneumonia. J Exp Med 203(13):2907-17. [PubMed: 17158962]  [MGI Ref ID J:124596]

Yamasaki S; Takase-Utsugi M; Ishikawa E; Sakuma M; Nishida K; Saito T; Kanagawa O. 2008. Selective impairment of FcepsilonRI-mediated allergic reaction in Gads-deficient mice. Int Immunol 20(10):1289-97. [PubMed: 18664516]  [MGI Ref ID J:140219]

Yu M; Tsai M; Tam SY; Jones C; Zehnder J; Galli SJ. 2006. Mast cells can promote the development of multiple features of chronic asthma in mice. J Clin Invest 116(6):1633-41. [PubMed: 16710480]  [MGI Ref ID J:110366]

Zabel BA; Nakae S; Zuniga L; Kim JY; Ohyama T; Alt C; Pan J; Suto H; Soler D; Allen SJ; Handel TM; Song CH; Galli SJ; Butcher EC. 2008. Mast cell-expressed orphan receptor CCRL2 binds chemerin and is required for optimal induction of IgE-mediated passive cutaneous anaphylaxis. J Exp Med 205(10):2207-20. [PubMed: 18794339]  [MGI Ref ID J:140110]

Zhou JS; Xing W; Friend DS; Austen KF; Katz HR. 2007. Mast cell deficiency in Kit(W-sh) mice does not impair antibody-mediated arthritis. J Exp Med 204(12):2797-802. [PubMed: 17998392]  [MGI Ref ID J:128512]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Breeding & Husbandry	Homozygotes are fertile and produce normal litters.
Mating System	Homozygote x Homozygote         (Female x Male)
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.

Supply Notes

Usually shipped between four and eight weeks of age. This strain is included in the Mouse Mutant Resource collection.

Control Information

	Control
	000664 C57BL/6J	(approximate)
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

      Purchasing Information
      JAX^® Mice Orders
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Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.