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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
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Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Inbred x Hemizygote (Female x Male) 01-MAR-06 Species laboratory mouse Generation N9+N14 (17-NOV-09) Donating Investigator Ronald Evans, The Salk Inst for Biological Studies Description
These transgenic mice express Cre recombinase under the control of the mouse Fabp4, fatty acid binding protein 4, adipocyte, promoter. Cre recombinase expression is detected in brown and white gonadal and subcutaneous adipose tissue. No expression is detected in skeletal muscle. When crossed with a strain containing a loxP site flanked sequence of interest, Cre-mediated recombination results in deletion of the targeted gene specifically in adipose tissue. Mice that are homozygous for the targeted mutation are viable. This strain represents an effective tool for generating tissue-specific targeted mutants.View cre expression characterization.
Development
A transgenic construct containing cre coding sequence under the control of the 5.4 kb Fabp4, fatty acid binding protein 4, adipocyte, promoter was introduced into unknown outbred donor eggs. The resulting male transgenic mice were crossed to B6.129-Ppargtm2Rev/J (Stock No. 004584) mice and subsequently crossed to C57BL/6J mice for 9 generations in the laboratory of Dr. Yaacov Barak at The Jackson Laboratory. The Ppargtm2Rev/J allele was bred out of the strain during the backcrossing to C57BL/6J.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Fabp4
003393 B6;SJL-Tg(aP2-SREBF1c)9884Reh/J 004100 FVB-Tg(AZIP/F)1Vsn/J View Strains carrying other alleles of Fabp4 (2 strains)
Additional Web Information
Genetic Quality Control Annual Report
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System
Cre Recombinase Expression
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Fabp4-cre)1Rev | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Ronald M Evans | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | aP2-CRE; | ||
| Mutation Made By | Weiman He, Texas A&M University | ||
| Site of Expression | adipose tissue | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Fabp4, fatty acid binding protein 4, adipocyte, mouse, laboratory | ||
| Gene Symbol and Name | Tg(Fabp4-cre)1Rev, transgene insertion 1, Ronald M Evans | ||
| Chromosome | UN | ||
| Gene Common Name(s) | aP2-CRE; | ||
| Driver Note | Fabp4 | ||
| Molecular Note | Sequence encoding cre recombinase was adjoined to the Fabp4 promoter, which is active in white and brown adipose tissue. Northern blot analysis and a functional assay showed the transgene to be abundantly expressed in brown and white adipose tissue. Significant expression was not detected in other tissues including skeletal muscle, liver, and heart. [MGI Ref ID J:73557] [MGI Ref ID J:88210] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
He W; Barak Y; Hevener A; Olson P; Liao D; Le J; Nelson M; Ong E; Olefsky JM; Evans RM. 2003. Adipose-specific peroxisome proliferator-activated receptor gamma knockout causes insulin resistance in fat and liver but not in muscle. Proc Natl Acad Sci U S A 100(26):15712-7. [PubMed: 14660788] [MGI Ref ID J:88210]
Additional References
Tg(Fabp4-cre)1Rev relatedBarak Y; Liao D; He W; Ong ES; Nelson MC; Olefsky JM; Boland R; Evans RM. 2002. Effects of peroxisome proliferator-activated receptor delta on placentation, adiposity, and colorectal cancer. Proc Natl Acad Sci U S A 99(1):303-8. [PubMed: 11756685] [MGI Ref ID J:73557]
Cernkovich ER; Deng J; Bond MC; Combs TP; Harp JB. 2008. Adipose-specific disruption of signal transducer and activator of transcription 3 increases body weight and adiposity. Endocrinology 149(4):1581-90. [PubMed: 18096662] [MGI Ref ID J:136030]
Cybulski N; Polak P; Auwerx J; Ruegg MA; Hall MN. 2009. mTOR complex 2 in adipose tissue negatively controls whole-body growth. Proc Natl Acad Sci U S A 106(24):9902-7. [PubMed: 19497867] [MGI Ref ID J:150064]
Genetic Resource Sciences at The Jackson Laboratory. 2008. Expression/Specificity patterns of Cre transgenes MGI Direct Data Submission :. [MGI Ref ID J:137887]
Hofmann SM; Zhou L; Perez-Tilve D; Greer T; Grant E; Wancata L; Thomas A; Pfluger PT; Basford JE; Gilham D; Herz J; Tschop MH; Hui DY. 2007. Adipocyte LDL receptor-related protein-1 expression modulates postprandial lipid transport and glucose homeostasis in mice. J Clin Invest 117(11):3271-82. [PubMed: 17948131] [MGI Ref ID J:127398]
Luo W; Cao J; Li J; He W. 2008. Adipose tissue-specific PPARgamma deficiency increases resistance to oxidative stress. Exp Gerontol 43(3):154-63. [PubMed: 18083318] [MGI Ref ID J:132506]
Mao J; Yang T; Gu Z; Heird WC; Finegold MJ; Lee B; Wakil SJ. 2009. aP2-Cre-mediated inactivation of acetyl-CoA carboxylase 1 causes growth retardation and reduced lipid accumulation in adipose tissues. Proc Natl Acad Sci U S A 106(41):17576-81. [PubMed: 19805143] [MGI Ref ID J:153681]
Pan D; Fujimoto M; Lopes A; Wang YX. 2009. Twist-1 is a PPARdelta-inducible, negative-feedback regulator of PGC-1alpha in brown fat metabolism. Cell 137(1):73-86. [PubMed: 19345188] [MGI Ref ID J:147973]
Polak P; Cybulski N; Feige JN; Auwerx J; Ruegg MA; Hall MN. 2008. Adipose-specific knockout of raptor results in lean mice with enhanced mitochondrial respiration. Cell Metab 8(5):399-410. [PubMed: 19046571] [MGI Ref ID J:143749]
Sabio G; Das M; Mora A; Zhang Z; Jun JY; Ko HJ; Barrett T; Kim JK; Davis RJ. 2008. A stress signaling pathway in adipose tissue regulates hepatic insulin resistance. Science 322(5907):1539-43. [PubMed: 19056984] [MGI Ref ID J:142035]
Terrand J; Bruban V; Zhou L; Gong W; El Asmar Z; May P; Zurhove K; Haffner P; Philippe C; Woldt E; Matz RL; Gracia C; Metzger D; Auwerx J; Herz J; Boucher P. 2009. LRP1 controls intracellular cholesterol storage and fatty acid synthesis through modulation of Wnt signaling. J Biol Chem 284(1):381-8. [PubMed: 18990694] [MGI Ref ID J:146007]
Urs S; Harrington A; Liaw L; Small D. 2006. Selective expression of an aP2/Fatty Acid Binding Protein 4-Cre transgene in non-adipogenic tissues during embryonic development. Transgenic Res 15(5):647-53. [PubMed: 16952017] [MGI Ref ID J:114599]
Yu IC; Lin HY; Liu NC; Wang RS; Sparks JD; Yeh S; Chang C. 2008. Hyperleptinemia without obesity in male mice lacking androgen receptor in adipose tissue. Endocrinology 149(5):2361-8. [PubMed: 18276764] [MGI Ref ID J:136018]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are bred as hemizygotes. Mating System Inbred x Hemizygote (Female x Male) 01-MAR-06 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Hemizygous for Tg(Fabp4-cre)1Rev
Pairs /Price (US dollars $) Pair Genotype $297.85 Hemizygous for Tg(Fabp4-cre)1Rev x Noncarrier $297.85 Noncarrier x Hemizygous for Tg(Fabp4-cre)1Rev $487.00 Hemizygous for Tg(Fabp4-cre)1Rev x Hemizygous for Tg(Fabp4-cre)1Rev
Additional Supply Details
| Pricing for International shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Hemizygous for Tg(Fabp4-cre)1Rev
Pairs /Price (US dollars $) Pair Genotype $387.30 Hemizygous for Tg(Fabp4-cre)1Rev x Noncarrier $387.30 Noncarrier x Hemizygous for Tg(Fabp4-cre)1Rev $633.10 Hemizygous for Tg(Fabp4-cre)1Rev x Hemizygous for Tg(Fabp4-cre)1Rev
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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