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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Inbred x Hemizygote (Female x Male) 01-MAR-06 Species laboratory mouse Generation N9+N17 (21-APR-11)
Generation DefinitionsDonating Investigator Ronald Evans, The Salk Inst for Biological Studies Description
These transgenic mice express Cre recombinase under the control of the mouse Fabp4, fatty acid binding protein 4, adipocyte, promoter. Cre recombinase expression is detected in brown and white gonadal and subcutaneous adipose tissue. No expression is detected in skeletal muscle. When crossed with a strain containing a loxP site flanked sequence of interest, Cre-mediated recombination results in deletion of the targeted gene specifically in adipose tissue. Mice that are homozygous for the targeted mutation are viable. This strain represents an effective tool for generating tissue-specific targeted mutants.View cre expression characterization.
Development
A transgenic construct containing cre coding sequence under the control of the 5.4 kb Fabp4, fatty acid binding protein 4, adipocyte, promoter was introduced into unknown outbred donor eggs. The resulting male transgenic mice were crossed to B6.129-Ppargtm2Rev/J (Stock No. 004584) mice and subsequently crossed to C57BL/6J mice for 9 generations in the laboratory of Dr. Yaacov Barak at The Jackson Laboratory. The Ppargtm2Rev/J allele was bred out of the strain during the backcrossing to C57BL/6J.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Fabp4
017560 B6.FVB-Tg(Fabp4-IKBKB*)49Hxu/J 003393 B6;SJL-Tg(aP2-SREBF1c)9884Reh/J 017321 C57BL/6-Tg(Fabp4-Dgat1)2Far/J View Strains carrying other alleles of Fabp4 (3 strains)
Additional Web Information
Genetic Quality Control Annual Report
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System
Cre Recombinase Expression
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Fabp4-cre)1Rev | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Ronald M Evans | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | FABP4-Cre; aP2-CRE; | ||
| Mutation Made By | Weiman He, Texas A&M University | ||
| Site of Expression | adipose tissue | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Fabp4, fatty acid binding protein 4, adipocyte, mouse, laboratory | ||
| Gene Symbol and Name | Tg(Fabp4-cre)1Rev, transgene insertion 1, Ronald M Evans | ||
| Chromosome | UN | ||
| Gene Common Name(s) | aP2-CRE; | ||
| Driver Note | Fabp4 | ||
| Molecular Note | Sequence encoding cre recombinase was adjoined to the Fabp4 promoter, which is active in white and brown adipose tissue. Northern blot analysis and a functional assay showed the transgene to be abundantly expressed in brown and white adipose tissue. Significant expression was not detected in other tissues including skeletal muscle, liver, and heart. [MGI Ref ID J:73557] [MGI Ref ID J:88210] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
He W; Barak Y; Hevener A; Olson P; Liao D; Le J; Nelson M; Ong E; Olefsky JM; Evans RM. 2003. Adipose-specific peroxisome proliferator-activated receptor gamma knockout causes insulin resistance in fat and liver but not in muscle. Proc Natl Acad Sci U S A 100(26):15712-7. [PubMed: 14660788] [MGI Ref ID J:88210]
Additional References
Tg(Fabp4-cre)1Rev relatedAhmadian M; Abbott MJ; Tang T; Hudak CS; Kim Y; Bruss M; Hellerstein MK; Lee HY; Samuel VT; Shulman GI; Wang Y; Duncan RE; Kang C; Sul HS. 2011. Desnutrin/ATGL is regulated by AMPK and is required for a brown adipose phenotype. Cell Metab 13(6):739-48. [PubMed: 21641555] [MGI Ref ID J:176083]
Barak Y; Liao D; He W; Ong ES; Nelson MC; Olefsky JM; Boland R; Evans RM. 2002. Effects of peroxisome proliferator-activated receptor delta on placentation, adiposity, and colorectal cancer. Proc Natl Acad Sci U S A 99(1):303-8. [PubMed: 11756685] [MGI Ref ID J:73557]
Cernkovich ER; Deng J; Bond MC; Combs TP; Harp JB. 2008. Adipose-specific disruption of signal transducer and activator of transcription 3 increases body weight and adiposity. Endocrinology 149(4):1581-90. [PubMed: 18096662] [MGI Ref ID J:136030]
Cybulski N; Polak P; Auwerx J; Ruegg MA; Hall MN. 2009. mTOR complex 2 in adipose tissue negatively controls whole-body growth. Proc Natl Acad Sci U S A 106(24):9902-7. [PubMed: 19497867] [MGI Ref ID J:150064]
Dusaulcy R; Rancoule C; Gres S; Wanecq E; Colom A; Guigne C; van Meeteren LA; Moolenaar WH; Valet P; Saulnier-Blache JS. 2011. Adipose-specific disruption of autotaxin enhances nutritional fattening and reduces plasma lysophosphatidic acid. J Lipid Res 52(6):1247-55. [PubMed: 21421848] [MGI Ref ID J:173584]
Ellis JM; Li LO; Wu PC; Koves TR; Ilkayeva O; Stevens RD; Watkins SM; Muoio DM; Coleman RA. 2010. Adipose acyl-CoA synthetase-1 directs fatty acids toward beta-oxidation and is required for cold thermogenesis. Cell Metab 12(1):53-64. [PubMed: 20620995] [MGI Ref ID J:162996]
Genetic Resource Sciences at The Jackson Laboratory. 2008. Expression/Specificity patterns of Cre transgenes MGI Direct Data Submission :. [MGI Ref ID J:137887]
Hesse D; Hommel A; Jaschke A; Moser M; Bernhardt U; Zahn C; Kluge R; Wittschen P; Gruber AD; Al-Hasani H; Joost HG; Schurmann A. 2010. Altered GLUT4 trafficking in adipocytes in the absence of the GTPase Arfrp1. Biochem Biophys Res Commun 394(4):896-903. [PubMed: 20230794] [MGI Ref ID J:159231]
Hofmann SM; Zhou L; Perez-Tilve D; Greer T; Grant E; Wancata L; Thomas A; Pfluger PT; Basford JE; Gilham D; Herz J; Tschop MH; Hui DY. 2007. Adipocyte LDL receptor-related protein-1 expression modulates postprandial lipid transport and glucose homeostasis in mice. J Clin Invest 117(11):3271-82. [PubMed: 17948131] [MGI Ref ID J:127398]
Hommel A; Hesse D; Volker W; Jaschke A; Moser M; Engel T; Bluher M; Zahn C; Chadt A; Ruschke K; Vogel H; Kluge R; Robenek H; Joost HG; Schurmann A. 2010. The ARF-like GTPase ARFRP1 is essential for lipid droplet growth and is involved in the regulation of lipolysis. Mol Cell Biol 30(5):1231-42. [PubMed: 20038528] [MGI Ref ID J:161742]
Kumar A; Lawrence JC Jr; Jung DY; Ko HJ; Keller SR; Kim JK; Magnuson MA; Harris TE. 2010. Fat cell-specific ablation of rictor in mice impairs insulin-regulated fat cell and whole-body glucose and lipid metabolism. Diabetes 59(6):1397-406. [PubMed: 20332342] [MGI Ref ID J:169357]
Lenz LS; Marx J; Chamulitrat W; Kaiser I; Grone HJ; Liebisch G; Schmitz G; Elsing C; Straub BK; Fullekrug J; Stremmel W; Herrmann T. 2011. Adipocyte-specific inactivation of Acyl-CoA synthetase fatty acid transport protein 4 (Fatp4) in mice causes adipose hypertrophy and alterations in metabolism of complex lipids under high fat diet. J Biol Chem 286(41):35578-87. [PubMed: 21808061] [MGI Ref ID J:177596]
Li P; Fan W; Xu J; Lu M; Yamamoto H; Auwerx J; Sears DD; Talukdar S; Oh D; Chen A; Bandyopadhyay G; Scadeng M; Ofrecio JM; Nalbandian S; Olefsky JM. 2011. Adipocyte NCoR knockout decreases PPARgamma phosphorylation and enhances PPARgamma activity and insulin sensitivity. Cell 147(4):815-26. [PubMed: 22078880] [MGI Ref ID J:178828]
Luo W; Cao J; Li J; He W. 2008. Adipose tissue-specific PPARgamma deficiency increases resistance to oxidative stress. Exp Gerontol 43(3):154-63. [PubMed: 18083318] [MGI Ref ID J:132506]
Mao J; Yang T; Gu Z; Heird WC; Finegold MJ; Lee B; Wakil SJ. 2009. aP2-Cre-mediated inactivation of acetyl-CoA carboxylase 1 causes growth retardation and reduced lipid accumulation in adipose tissues. Proc Natl Acad Sci U S A 106(41):17576-81. [PubMed: 19805143] [MGI Ref ID J:153681]
Martens K; Bottelbergs A; Baes M. 2010. Ectopic recombination in the central and peripheral nervous system by aP2/FABP4-Cre mice: implications for metabolism research. FEBS Lett 584(5):1054-8. [PubMed: 20138876] [MGI Ref ID J:157524]
Minamino T; Orimo M; Shimizu I; Kunieda T; Yokoyama M; Ito T; Nojima A; Nabetani A; Oike Y; Matsubara H; Ishikawa F; Komuro I. 2009. A crucial role for adipose tissue p53 in the regulation of insulin resistance. Nat Med 15(9):1082-7. [PubMed: 19718037] [MGI Ref ID J:154323]
Pan D; Fujimoto M; Lopes A; Wang YX. 2009. Twist-1 is a PPARdelta-inducible, negative-feedback regulator of PGC-1alpha in brown fat metabolism. Cell 137(1):73-86. [PubMed: 19345188] [MGI Ref ID J:147973]
Polak P; Cybulski N; Feige JN; Auwerx J; Ruegg MA; Hall MN. 2008. Adipose-specific knockout of raptor results in lean mice with enhanced mitochondrial respiration. Cell Metab 8(5):399-410. [PubMed: 19046571] [MGI Ref ID J:143749]
Pospisilik JA; Schramek D; Schnidar H; Cronin SJ; Nehme NT; Zhang X; Knauf C; Cani PD; Aumayr K; Todoric J; Bayer M; Haschemi A; Puviindran V; Tar K; Orthofer M; Neely GG; Dietzl G; Manoukian A; Funovics M; Prager G; Wagner O; Ferrandon D; Aberger F; HuiCC; Esterbauer H; Penninger JM. 2010. Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. Cell 140(1):148-60. [PubMed: 20074523] [MGI Ref ID J:166285]
Sabio G; Das M; Mora A; Zhang Z; Jun JY; Ko HJ; Barrett T; Kim JK; Davis RJ. 2008. A stress signaling pathway in adipose tissue regulates hepatic insulin resistance. Science 322(5907):1539-43. [PubMed: 19056984] [MGI Ref ID J:142035]
Singh R; Xiang Y; Wang Y; Baikati K; Cuervo AM; Luu YK; Tang Y; Pessin JE; Schwartz GJ; Czaja MJ. 2009. Autophagy regulates adipose mass and differentiation in mice. J Clin Invest 119(11):3329-39. [PubMed: 19855132] [MGI Ref ID J:154591]
Starkey JD; Yamamoto M; Yamamoto S; Goldhamer DJ. 2011. Skeletal muscle satellite cells are committed to myogenesis and do not spontaneously adopt nonmyogenic fates. J Histochem Cytochem 59(1):33-46. [PubMed: 21339173] [MGI Ref ID J:169754]
Terrand J; Bruban V; Zhou L; Gong W; El Asmar Z; May P; Zurhove K; Haffner P; Philippe C; Woldt E; Matz RL; Gracia C; Metzger D; Auwerx J; Herz J; Boucher P. 2009. LRP1 controls intracellular cholesterol storage and fatty acid synthesis through modulation of Wnt signaling. J Biol Chem 284(1):381-8. [PubMed: 18990694] [MGI Ref ID J:146007]
Urs S; Harrington A; Liaw L; Small D. 2006. Selective expression of an aP2/Fatty Acid Binding Protein 4-Cre transgene in non-adipogenic tissues during embryonic development. Transgenic Res 15(5):647-53. [PubMed: 16952017] [MGI Ref ID J:114599]
Urs S; Venkatesh D; Tang Y; Henderson T; Yang X; Friesel RE; Rosen CJ; Liaw L. 2010. Sprouty1 is a critical regulatory switch of mesenchymal stem cell lineage allocation. FASEB J 24(9):3264-73. [PubMed: 20410440] [MGI Ref ID J:163565]
Wang ZV; Deng Y; Wang QA; Sun K; Scherer PE. 2010. Identification and characterization of a promoter cassette conferring adipocyte-specific gene expression. Endocrinology 151(6):2933-9. [PubMed: 20363877] [MGI Ref ID J:160881]
Wueest S; Rapold RA; Schoenle EJ; Konrad D. 2010. Fas activation in adipocytes impairs insulin-stimulated glucose uptake by reducing Akt. FEBS Lett 584(19):4187-92. [PubMed: 20828573] [MGI Ref ID J:164421]
Wueest S; Rapold RA; Schumann DM; Rytka JM; Schildknecht A; Nov O; Chervonsky AV; Rudich A; Schoenle EJ; Donath MY; Konrad D. 2010. Deletion of Fas in adipocytes relieves adipose tissue inflammation and hepatic manifestations of obesity in mice. J Clin Invest 120(1):191-202. [PubMed: 19955656] [MGI Ref ID J:156776]
Yu IC; Lin HY; Liu NC; Wang RS; Sparks JD; Yeh S; Chang C. 2008. Hyperleptinemia without obesity in male mice lacking androgen receptor in adipose tissue. Endocrinology 149(5):2361-8. [PubMed: 18276764] [MGI Ref ID J:136018]
Zhang Y; Goldman S; Baerga R; Zhao Y; Komatsu M; Jin S. 2009. Adipose-specific deletion of autophagy-related gene 7 (atg7) in mice reveals a role in adipogenesis. Proc Natl Acad Sci U S A 106(47):19860-5. [PubMed: 19910529] [MGI Ref ID J:154736]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are bred as hemizygotes. Mating System Inbred x Hemizygote (Female x Male) 01-MAR-06 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $261.00 Female or Male Hemizygous for Tg(Fabp4-cre)1Rev
Pairs /Price (US dollars $) Pair Genotype $321.00 Hemizygous for Tg(Fabp4-cre)1Rev x Noncarrier $321.00 Noncarrier x Hemizygous for Tg(Fabp4-cre)1Rev $522.00 Hemizygous for Tg(Fabp4-cre)1Rev x Hemizygous for Tg(Fabp4-cre)1Rev Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $339.30 Female or Male Hemizygous for Tg(Fabp4-cre)1Rev
Pairs /Price (US dollars $) Pair Genotype $417.30 Hemizygous for Tg(Fabp4-cre)1Rev x Noncarrier $417.30 Noncarrier x Hemizygous for Tg(Fabp4-cre)1Rev $678.60 Hemizygous for Tg(Fabp4-cre)1Rev x Hemizygous for Tg(Fabp4-cre)1Rev Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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