Strain Name: |
B6.Cg-Tg(Fabp4-cre)1Rev/J |
|---|---|
Stock Number: |
005069 |
Availability: | Repository- Live |
Price and Supply Information | |
General Terms and Conditions |
| Genes & Alleles | Fabp4; cre; Tg(Fabp4-cre)1Rev; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Inbred x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Ronald Evans, The Salk Inst for Biological Studies Generation N9+N10 (27-APR-08) Strain Description
These transgenic mice express Cre recombinase under the control of the mouse Fabp4, fatty acid binding protein 4, adipocyte, promoter. Cre recombinase expression is detected in brown and white gonadal and subcutaneous adipose tissue. No expression is detected in skeletal muscle. When crossed with a strain containing a loxP site flanked sequence of interest, Cre-mediated recombination results in deletion of the targeted gene specifically in adipose tissue. Mice that are homozygous for the targeted mutation are viable. This strain represents an effective tool for generating tissue-specific targeted mutants.Strain Development
A transgenic construct containing cre coding sequence under the control of the 5.4 kb Fabp4, fatty acid binding protein 4, adipocyte, promoter was introduced into unknown outbred donor eggs. The resulting male transgenic mice were crossed to B6.129-Ppargtm2Rev/J (Stock No. 004584) mice and subsequently crossed to C57BL/6J mice for 9 generations in the laboratory of Dr. Yaacov Barak at The Jackson Laboratory. The Ppargtm2Rev/J allele was bred out of the strain during the backcrossing to C57BL/6J.
Gene & Allele Details
| Allele Symbol | Tg(Fabp4-cre)1Rev | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Ronald M Evans | ||
| Common Name(s) | aP2-CRE; | ||
| Mutation Made By | Weiman He, Institute of Biosciences and Technology | ||
| Site of Expression | adipose tissue | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Fabp4, fatty acid binding protein 4, adipocyte, mouse, laboratory | ||
| Molecular Note | Sequence encoding cre recombinase was adjoined to the Fabp4 promoter, which is active in white and brown adipose tissue. Northern blot analysis and a functional assay showed the transgene to be abundantly expressed in brown and white adipose tissue. Significant expression was not detected in other tissues including skeletal muscle, liver, and heart. [MGI Ref ID J:73557] [MGI Ref ID J:88210] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Genotyping Protocols
Generic Cre
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice are bred as hemizygotes. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Fabp4
003393 B6;SJL-Tg(aP2-SREBF1c)9884Reh/J 004100 FVB-Tg(AZIP/F)1Vsn/J View Strains carrying other alleles of Fabp4 (2 strains)
Additional Web Information
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
Genetic Quality Control Annual Report
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System (Cre Recombinase Expression)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
References
Selected Reference(s)
Additional ReferencesHe W; Barak Y; Hevener A; Olson P; Liao D; Le J; Nelson M; Ong E; Olefsky JM; Evans RM. 2003. Adipose-specific peroxisome proliferator-activated receptor gamma knockout causes insulin resistance in fat and liver but not in muscle. Proc Natl Acad Sci U S A 100(26):15712-7. [PubMed: 14660788] [MGI Ref ID J:88210]
Price and Supply Information
| Strain Name: | B6.Cg-Tg(Fabp4-cre)1Rev/J |
| Stock Number: | 005069 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
International
| Price(s) in US dollars ($) | Genotype(s) Provided | ||||
|---|---|---|---|---|---|
| Individual Mouse Price | $307.40 | Hemizygous for Tg(Fabp4-cre)1Rev | |||
| Pair | $375.30 | Hemizygous for Tg(Fabp4-cre)1Rev x Noncarrier | |||
| Pair | $375.30 | Noncarrier x Hemizygous for Tg(Fabp4-cre)1Rev | |||
| Pair | $614.70 | Hemizygous for Tg(Fabp4-cre)1Rev x Hemizygous for Tg(Fabp4-cre)1Rev | |||
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-207-288-5845. Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Contact Information
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