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- Description
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- Genes & alleles
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Description
Strain Information
Former Names C57BL/6J-Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck/J (Changed: 11-AUG-06 ) C57BL/6J-Tg(Csf1r-GFP, NGFR/FKBP12)2Bck/J (Changed: 15-DEC-04 ) Type Mutant Strain; Transgenic; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Generation N6F?+N1F11 (31-DEC-07) Donating Investigator Donald Cohen, University of Kentucky Description
These transgenic mice have an inducible Fas suicide/ apoptotic system driven by the mouse Csf1r, colony stimulating factor 1 receptor promoter. The transgene insert contains a mutant human FK506 binding protein 1A, 12kDa (FKBP12) which preferentially binds the dimerization drug AP20187. Enhanced Green Fluorescent Protein (EGFP) fluorescence and transgene expression is detected in 78% of isolated peritoneal cells. EGFP fluorescence is variable among tissues (B- and T-cells do not express EGFP). Administration of the dimerizing reagent, AP20187, induces apoptosis in macrophages and dendritic cells (intravenous injection of dimerizer is recommended, since the intraperitoneal route can elicit peritoneal adhesions). In treated mice, EGFP fluorescing peritoneal and bone marrow macrophage numbers are depleted by more than 90%, and macrophage numbers in blood, spleen, lung and thymus by more than 70%. 7 days after cessation of treatment, the EGFP fluorescing macrophage and dendritic cell populations rebound. Homozygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.Development
An FKBP-Fas suicide construct (containing an IRES sequence, human low affinity nerve growth factor receptor [deltaLNGFR], 2 copies of the 12kDa human FK506 binding protein 1A [FKBP12], and the intracellular domain region of the Fas gene) was inserted immediately downstream of the Enhanced Green Fluorescent Protein (EGFP, Clonetech) gene. This entire construct was placed under the control of the mouse colony stimulating factor 1 receptor (Csf1r) promoter. The resulting 11.1 kb transgene (termed Macrophage Fas-Induced Apoptosis or MaFIA) was introduced into C57BL/6 donor eggs. The resulting male transgenic mice (founder line 2) were bred to C57BL/6 mice for 6 generations prior to arrival at The Jackson Laboratory.
Control Information
| Control | ||
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| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (170 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck/Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck
C57BL/6
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:89035)
- mice homozygous for the transgene are viable and exhibit no gross physical or behavioral abnormalities
This mouse can be used to support research in many areas including:
GFP relatedResearch Tools
Apoptosis Research
Cell Biology Research
Fluorescent Proteins
Hematological Research
Immunology and Inflammation Research
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck | ||
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| Allele Name | transgene insertion 2, Donald A Cohen | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | MaFIA (Macrophage Fas-Induced Apoptosis); Mafia; | ||
| Mutation Made By | Sandra Hale-Burnett, Brigham Young University | ||
| Strain of Origin | C57BL/6 | ||
| Site of Expression | Expression of GFP and the NGFR/FKBP12 transgene is detected in 78% of isolated peritoneal cells. GFP expression is variable among tissues. B and T cells do not express GFP. Administration of AP20187 induces apoptosis inmacrophages and dendritic cells. | ||
| Expressed Gene | NGFR, nerve growth factor receptor (TNFR superfamily, member 16), human | ||
| Expressed Gene | TNFRSF6B, tumor necrosis factor receptor superfamily, member 6b, decoy, human | ||
| Expressed Gene | FKBP1A, FK506 binding protein 1A, 12kDa, human | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Csf1r, colony stimulating factor 1 receptor, mouse, laboratory | ||
| General Note |
Homozygous mutant mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. The transgene is expressed constitutively in macrophages and dendritic cells and permits conditional ablation of these cellpopulations in the mouse. Oligomerization of the fusion protein by binding of a bivalent FK506 analog causes them to undergo apoptosis via the Fas-mediated pathway. In transgenic mice treated with the dimerizing reagent AP20187, EGFP fluorescing peritoneal and bone marrow macrophage numbers are depleted by more than 90%, and macrophage numbers in blood, spleen, lung and thymus by more than 70%. Seven days after cessation of treatment, the EGFP fluorescing macrophage and dendritic cell populations rebound. | ||
| Molecular Note | The transgene's bicistronic transcript, expressed from the promoter of the macrophage-expressed colony stimulating factor 1 receptor gene, encodes enhanced green fluorescent protein (EGFP) and a conditionally apoptotic fusion protein. The latter comprises the extracellular and transmembrane domains of the human low affinity nerve growth factor receptor, two tandem copies of the human FK506 binding protein, and the intracellular domain of human TNFRSF6 - the Fas receptor protein - and is translated from an internal RNA expression signal (IRES) inserted at the translation start codon of the nerve growth factor receptor (NGFR) coding sequence. [MGI Ref ID J:89035] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), MCA, vers. 2
Fluorescent Proteins (Generic GFP), STD PCR, vers. 1
Fluorescent Proteins -- Generic GFP, QPCR, vers. 5
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Burnett SH; Kershen EJ; Zhang J; Zeng L; Straley SC; Kaplan AM; Cohen DA. 2004. Conditional macrophage ablation in transgenic mice expressing a Fas-based suicide gene. J Leukoc Biol 75(4):612-23. [PubMed: 14726498] [MGI Ref ID J:89035]
Additional References
Luther SA; Lopez T; Bai W; Hanahan D; Cyster JG. 2000. BLC expression in pancreatic islets causes B cell recruitment and lymphotoxin-dependent lymphoid neogenesis. Immunity 12(5):471-81. [PubMed: 10843380] [MGI Ref ID J:110548]
Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck relatedQualls JE; Kaplan AM; van Rooijen N; Cohen DA. 2006. Suppression of experimental colitis by intestinal mononuclear phagocytes. J Leukoc Biol 80(4):802-15. [PubMed: 16888083] [MGI Ref ID J:113033]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, transgenic mice can be bred together, to wildtype siblings, or to C57BL/6J wildtype mice. Mating System Homozygote x Homozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $104.80 Female or Male Homozygous for Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $209.60 Homozygous for Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck x Homozygous for Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $136.30 Female or Male Homozygous for Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $272.50 Homozygous for Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck x Homozygous for Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
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