Strain Name: |
C57BL/6-Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck/J |
|---|---|
Stock Number: |
005070 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
C57BL/6J-Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck/J (Changed: 11-AUG-06
) |
|
C57BL/6J-Tg(Csf1r-GFP, NGFR/FKBP12)2Bck/J (Changed: 15-DEC-04
) | |
| Genes & Alleles | Csf1r; FKBP1A; GFP; NGFR; TNFRSF6B; Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Strain Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Donald Cohen, University of Kentucky Generation N6F?+N1F11 (31-DEC-07) Strain Description
These transgenic mice have an inducible Fas suicide/ apoptotic system driven by the mouse Csf1r, colony stimulating factor 1 receptor promoter. The transgene insert contains a mutant human FK506 binding protein 1A, 12kDa (FKBP12) which preferentially binds the dimerization drug AP20187. Enhanced Green Fluorescent Protein (EGFP) fluorescence and transgene expression is detected in 78% of isolated peritoneal cells. EGFP fluorescence is variable among tissues (B- and T-cells do not express EGFP). Administration of the dimerizing reagent, AP20187, induces apoptosis in macrophages and dendritic cells (intravenous injection of dimerizer is recommended, since the intraperitoneal route can elicit peritoneal adhesions). In treated mice, EGFP fluorescing peritoneal and bone marrow macrophage numbers are depleted by more than 90%, and macrophage numbers in blood, spleen, lung and thymus by more than 70%. 7 days after cessation of treatment, the EGFP fluorescing macrophage and dendritic cell populations rebound. Homozygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. As this strain is known to contain multiple transgene insertion sites, the donating investigator recommends that all offspring be screened for EGFP expression by flow cytometry.Strain Development
An FKBP-Fas suicide construct (containing an IRES sequence, human low affinity nerve growth factor receptor [deltaLNGFR], 2 copies of the 12kDa human FK506 binding protein 1A [FKBP12], and the intracellular domain region of the Fas gene) was inserted immediately downstream of the Enhanced Green Fluorescent Protein (EGFP, Clonetech) gene. This entire construct was placed under the control of the mouse colony stimulating factor 1 receptor (Csf1r) promoter. The resulting 11.1 kb transgene (termed Macrophage Fas-Induced Apoptosis or MaFIA) was introduced into C57BL/6 donor eggs. The resulting male transgenic mice (founder line 2) were bred to C57BL/6 mice for 6 generations prior to arrival at The Jackson Laboratory.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck | ||
|---|---|---|---|
| Allele Name | transgene insertion 2, Donald A Cohen | ||
| Common Name(s) | MaFIA (Macrophage Fas-Induced Apoptosis); | ||
| Mutation Made By | Sandra Hale-Burnett, Brigham Young University | ||
| Strain of Origin | C57BL/6 | ||
| Site of Expression | Expression of GFP and the NGFR/FKBP12 transgene is detected in 78% of isolated peritoneal cells. GFP expression is variable among tissues. B and T cells do not express GFP. Administration of AP20187 induces apoptosis inmacrophages and dendritic cells. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Expressed Gene | NGFR, nerve growth factor receptor (TNFR superfamily, member 16), human | ||
| Expressed Gene | FKBP1A, FK506 binding protein 1A, 12kDa, human | ||
| Expressed Gene | TNFRSF6B, tumor necrosis factor receptor superfamily, member 6b, decoy, human | ||
| Promoter | Csf1r, colony stimulating factor 1 receptor, mouse, laboratory | ||
| General Note |
Homozygous mutant mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. The transgene is expressed constitutively in macrophages and dendritic cells and permits conditional ablation of these cellpopulations in the mouse. Oligomerization of the fusion protein by binding of a bivalent FK506 analog causes them to undergo apoptosis via the Fas-mediated pathway. In transgenic mice treated with the dimerizing reagent AP20187, EGFP fluorescing peritoneal and bone marrow macrophage numbers are depleted by more than 90%, and macrophage numbers in blood, spleen, lung and thymus by more than 70%. Seven days after cessation of treatment, the EGFP fluorescing macrophage and dendritic cell populations rebound. | ||
| Molecular Note | The transgene's bicistronic transcript, expressed from the promoter of the macrophage-expressed colony stimulating factor 1 receptor gene, encodes enhanced green fluorescent protein (EGFP) and a conditionally apoptotic fusion protein. The latter comprises the extracellular and transmembrane domains of the human low affinity nerve growth factor receptor, two tandem copies of the human FK506 binding protein, and the intracellular domain of human TNFRSF6 - the Fas receptor protein - and is translated from an internal RNA expression signal (IRES) inserted at the translation start codon of the nerve growth factor receptor (NGFR) coding sequence. [J:89035] | ||
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Fluorescent Proteins
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, transgenic mice can be bred together, to wildtype siblings, or to C57BL/6J wildtype mice. The donating investigator recommends that all offspring be screened for EGFP expression by flow cytometry. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
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Additional Web Information
Fluorescent Proteins/lacZ Systems
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
GFP relatedResearch Tools
Apoptosis Research
Cell Biology Research
Fluorescent Proteins
Hematological Research
Immunology and Inflammation Research
Research Tools
Fluorescent Proteins
References
Selected Reference(s)
Additional ReferencesBurnett SH; Kershen EJ; Zhang J; Zeng L; Straley SC; Kaplan AM; Cohen DA. 2004. Conditional macrophage ablation in transgenic mice expressing a Fas-based suicide gene. J Leukoc Biol 75(4):612-23. [PubMed: 14726498] [J:89035]
Price and Supply Information
| Strain Name: | C57BL/6-Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck/J |
| Stock Number: | 005070 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. No age specifications accepted. Colony sized to produce minimal quantities (typically up to 6 mice) upon order receipt; one order per strain. Expected delivery: 1-3 months. Larger quantities or custom orders arranged upon request. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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