Strain Name: |
C57BL/6-Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck/J |
|---|---|
Stock Number: |
005070 |
Availability: | Repository- Live |
Price and Supply Information | |
General Terms and Conditions |
| Former Name |
C57BL/6J-Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck/J (Changed: 11-AUG-06
) |
|
C57BL/6J-Tg(Csf1r-GFP, NGFR/FKBP12)2Bck/J (Changed: 15-DEC-04
) | |
| Genes & Alleles | Csf1r; FKBP1A; GFP; NGFR; TNFRSF6B; Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Strain Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Donald Cohen, University of Kentucky Generation N6F?+N1F11 (31-DEC-07) Strain Description
These transgenic mice have an inducible Fas suicide/ apoptotic system driven by the mouse Csf1r, colony stimulating factor 1 receptor promoter. The transgene insert contains a mutant human FK506 binding protein 1A, 12kDa (FKBP12) which preferentially binds the dimerization drug AP20187. Enhanced Green Fluorescent Protein (EGFP) fluorescence and transgene expression is detected in 78% of isolated peritoneal cells. EGFP fluorescence is variable among tissues (B- and T-cells do not express EGFP). Administration of the dimerizing reagent, AP20187, induces apoptosis in macrophages and dendritic cells (intravenous injection of dimerizer is recommended, since the intraperitoneal route can elicit peritoneal adhesions). In treated mice, EGFP fluorescing peritoneal and bone marrow macrophage numbers are depleted by more than 90%, and macrophage numbers in blood, spleen, lung and thymus by more than 70%. 7 days after cessation of treatment, the EGFP fluorescing macrophage and dendritic cell populations rebound. Homozygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.Strain Development
An FKBP-Fas suicide construct (containing an IRES sequence, human low affinity nerve growth factor receptor [deltaLNGFR], 2 copies of the 12kDa human FK506 binding protein 1A [FKBP12], and the intracellular domain region of the Fas gene) was inserted immediately downstream of the Enhanced Green Fluorescent Protein (EGFP, Clonetech) gene. This entire construct was placed under the control of the mouse colony stimulating factor 1 receptor (Csf1r) promoter. The resulting 11.1 kb transgene (termed Macrophage Fas-Induced Apoptosis or MaFIA) was introduced into C57BL/6 donor eggs. The resulting male transgenic mice (founder line 2) were bred to C57BL/6 mice for 6 generations prior to arrival at The Jackson Laboratory.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck | ||
|---|---|---|---|
| Allele Name | transgene insertion 2, Donald A Cohen | ||
| Common Name(s) | MaFIA (Macrophage Fas-Induced Apoptosis); Mafia; | ||
| Mutation Made By | Sandra Hale-Burnett, Brigham Young University | ||
| Strain of Origin | C57BL/6 | ||
| Site of Expression | Expression of GFP and the NGFR/FKBP12 transgene is detected in 78% of isolated peritoneal cells. GFP expression is variable among tissues. B and T cells do not express GFP. Administration of AP20187 induces apoptosis inmacrophages and dendritic cells. | ||
| Expressed Gene | NGFR, nerve growth factor receptor (TNFR superfamily, member 16), human | ||
| Expressed Gene | TNFRSF6B, tumor necrosis factor receptor superfamily, member 6b, decoy, human | ||
| Expressed Gene | FKBP1A, FK506 binding protein 1A, 12kDa, human | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Csf1r, colony stimulating factor 1 receptor, mouse, laboratory | ||
| General Note |
Homozygous mutant mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. The transgene is expressed constitutively in macrophages and dendritic cells and permits conditional ablation of these cellpopulations in the mouse. Oligomerization of the fusion protein by binding of a bivalent FK506 analog causes them to undergo apoptosis via the Fas-mediated pathway. In transgenic mice treated with the dimerizing reagent AP20187, EGFP fluorescing peritoneal and bone marrow macrophage numbers are depleted by more than 90%, and macrophage numbers in blood, spleen, lung and thymus by more than 70%. Seven days after cessation of treatment, the EGFP fluorescing macrophage and dendritic cell populations rebound. | ||
| Molecular Note | The transgene's bicistronic transcript, expressed from the promoter of the macrophage-expressed colony stimulating factor 1 receptor gene, encodes enhanced green fluorescent protein (EGFP) and a conditionally apoptotic fusion protein. The latter comprises the extracellular and transmembrane domains of the human low affinity nerve growth factor receptor, two tandem copies of the human FK506 binding protein, and the intracellular domain of human TNFRSF6 - the Fas receptor protein - and is translated from an internal RNA expression signal (IRES) inserted at the translation start codon of the nerve growth factor receptor (NGFR) coding sequence. [MGI Ref ID J:89035] | ||
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Genotyping Protocols
Fluorescent Proteins (Generic GFP)
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, transgenic mice can be bred together, to wildtype siblings, or to C57BL/6J wildtype mice. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (145 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
GFP relatedResearch Tools
Apoptosis Research
Cell Biology Research
Fluorescent Proteins
Hematological Research
Immunology and Inflammation Research
Research Tools
Fluorescent Proteins
References
Selected Reference(s)
Additional ReferencesBurnett SH; Kershen EJ; Zhang J; Zeng L; Straley SC; Kaplan AM; Cohen DA. 2004. Conditional macrophage ablation in transgenic mice expressing a Fas-based suicide gene. J Leukoc Biol 75(4):612-23. [PubMed: 14726498] [MGI Ref ID J:89035]
Price and Supply Information
| Strain Name: | C57BL/6-Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck/J |
| Stock Number: | 005070 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
International
| Price(s) in US dollars ($) | Genotype(s) Provided | ||||
|---|---|---|---|---|---|
| Individual Mouse Price | $136.30 | Homozygous for Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck | |||
| Pair | $272.50 | Homozygous for Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck x Homozygous for Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck | |||
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-207-288-5845. Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE requires an MTA prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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