Strain Name: |
NOD.Cg-Tg(tetO-EGFP/FADD)1Doi/DoiJ |
|---|---|
Stock Number: |
005076 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Former Name |
NOD.Cg-Tg(tetO-GFP/FADD)1Doi/DoiJ (Changed: 24-JAN-05
) |
|
NOD.Cg-Tg(tetO-GFP/FADD)/DoiJ (Changed: 13-JAN-05
) | |
| Genes & Alleles | FADD; GFP; Tg(tetO-EGFP/FADD)1Doi; tetO; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Background Strain NOD/ShiLtJ Donor Strain B6SJLF2 Donating Investigator Christophe Benoist, Joslin Diabetes Center H2 Haplotype g7 Appearance
albino, pink-eyed
Related Genotype: A/? Tyrc/TyrcStrain Description
Mice hemizygous for this transgenic insert are viable, fertile, normal in size. Diabetes incidence and onset is similar to that observed in NOD inbred mice, but otherwise, the mice do not display any gross physical or behavioral abnormalities. These transgenic mutant mice may be mated to strains carrying either rtTA (reverse tetracycline trans-activator protein) or tTA (the tetracycline trans-activator) transgenes regulated by tissue specific promoters. In the bi-transgenic offspring of such crosses, tissue-specific expression of the EGFP/FADD fusion protein can be either induced (in rtTA transgenic mice) or suppressed (in tTA transgenice mice) by administration of the tetracycline analog, doxycycline (dox). Dox may be administered in the animals? water supply.Double transgenic mice generated by intercrossing Tg(tetO-EGFP/FADD)1Doi transgenic mice with NOD.Cg-Tg(Ins2-rtTA)2Doi/DoiJ (Stock No. 004602) transgenic mice express the EGFP/FADD fusion protein exclusively in the pancreatic b-cells in the presence of dox. Strikingly, only about 35% of the b-cells express the EGFP/FADD fusion protein in the presence of dox. (Vence L, Mathis D, Benoist C. 2004, graduate thesis, unpublished). Alternatively, when Tg(tetO-EGFP/FADD)1Doi transgenic mice are crossed with NOD.Cg-Tg(Ins2-tTA)1Doi/DoiJ (Stock No. 004937) the EGFP/FADD fusion protein is expressed in pancreatic b-cells, in the absence of dox, and its expression can be down-regulated by dox administration.
These two double transgenic systems can be used to study the influence of FADD in apoptosis of pancreatic b-cells.
Strain Development
The fusion transgene tetO-EGFP/FADD is under the control of the tetracycline responsive promoter (TRE; tetO), and encodes a Fas associated death domain (FADD) molecule in which a full-length enhanced green florescent protein (EGFP) replaces the FADD death effector domain (amino acids 1-79) (Vence L, Mathis D, Benoist C. 2004, graduate thesis, unpublished). Wajant et al, 1998, provided the EGFP/FADD fusion product. The tetO-EGFP/FADD transgenic construct was microinjected into B6SJLF2 embryos, and the resulting transgenice founder was backcrossed onto the NOD background. In 2004, the Type 1 Diabetes Resource received NOD.Cg-Tg(tetO-EGFP/FADD)1Doi/DoiJ at generation N15. These animals were subsequently backcrossed once to NOD/ShiLtJ, and are maintained as Tg/? x Tg/?
Gene & Allele Details
| Allele Symbol | Tg(tetO-EGFP/FADD)1Doi | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Christophe Benoist | ||
| Mutation Made By | Christophe Benoist, Joslin Diabetes Center | ||
| Strain of Origin | (C57BL/6 x SJL)F2 | ||
| Site of Expression | May be mated to strains carrying either rtTA or tTA transgenes regulated by tissue specific promoters for the study of tissue-specific expression of the EGFP/FADD fusion protein either induced (by the rtTA construct) or suppressed (by the tTA construct) by respective administration or removal of the tetracycline analog, doxycycline (Dox). | ||
| Expressed Gene | FADD, Fas (TNFRSF6)-associated via death domain, human | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | tetO, tet operator, | ||
| Molecular Note | The transgene encodes a fusion protein consisting of a truncated, dominant-negative mutant Fas-associated death domain protein in which amino acids 1-79, constituting the death effector domain, have been replaced by the full-length enhanced green fluorescent protein. The coding sequence, preceded by a minimal CMV promoter and a rabbit beta-globin intron and followed by the beta-globin polyadenylation signal, resides downstream of seven copies of the tet operator (tetO). TetO, when present in cells also carrying a tetracycline transactivator (tTA) or reverse transactivator (rtTA) transgene, is inactivated or activated, respectively, by the administration of tetracycline. [MGI Ref ID J:94935] [MGI Ref ID J:95005] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001976 NOD/ShiLtJ | ||
| Considerations for Choosing Controls | ||
Colony Maintenance
| Diet Information | LabDiet® 5K52/5K67 |
|---|
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (145 strains)
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Tet Expression Systems
Research Applications
This mouse can be used to support research in many areas including:
GFP relatedApoptosis Research
Death Receptors
Diabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains (NOD Transgenics)
Research Tools
Apoptosis Research
Diabetes and Obesity Research
Fluorescent Proteins
Tet Expression Systems (tTA/rtTA Responsive Strains)
Research Tools
Fluorescent Proteins
References
Additional ReferencesPrice and Supply Information
| Strain Name: | NOD.Cg-Tg(tetO-EGFP/FADD)1Doi/DoiJ |
| Stock Number: | 005076 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
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View JAX® Mice & Services Conditions of Use.
Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.
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