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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Background Strain NOD Donor Strain (129X1/SvJ x 129S1/Sv)F1-Kitl+ H2 Haplotype g7 Generation N20F?+N1F4pN1
Generation DefinitionsDonating Investigator H. Michael Dosch, The Hospital for Sick Children Appearance
pink-eyed, albino
Related Genotype: A/? Tyrc/TyrcDescription
Ica1tm1Mdos homozygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. There is no detectable expression of Ica1 by western blot analysis. Ica1tm1Mdos homozygous animals develop mild salivary gland infiltrations with progression of disease 55%-65% slower than progression of submandibular gland monouclear foci in wildtype and heterozygote controls. Dacryodenitis was undetectable in 35-40 week old male Ica1tm1Mdos homozygous mice compared to 64% of wildtype and 58% of heterozygotes similarly aged (Winer et al., 2002). Development of spontaneous diabetes is slightly delayed in this stock (by 4 weeks) and incidence is modestly diminished (65% incidence in females after 36 weeks versus 85% in wild-type NOD females). Ica1tm1Mdos homozygous females are significantly protected from cyclophosphomide (CY) induced diabetes (10% in females post CY treatment versus 70% incidence in female heterozygote controls). Many unmanipulated Ica1tm1Mdos homozygous females not diagnosed as diabetic died unexpectedly of unknown causes, resulting in a 35% death rate by 50 weeks of age (Winer et al., 2002).This model provides a tool for studying the role of Ica1 as a beta cell autoantigen in the NOD mouse model of Type 1 diabetes and sialadenitis and dacryoadenitis.
Development
A construct containing a tTA-neo cassette replaced the Tep69 epitope of exon 2 region of Islet cell antigen 1,Ica1, commonly referred to as Ica69 and cloned from a 129/Sv mouse genomic library and transfected into R1 ((129X1/SvJ x 129S1/Sv)F1-Kitl+) embryonic stem cells (ES cells). These ES cells were injected into NOD/Lt blastocysts. To establish a colony with germ line transmission, chimeric mice were backcrossed to NOD/Lt. In 2004, The Jackson Laboratory received NOD.129-Ica1tm1Mdos/MdosJ at generation N20. These animals were subsequently backcrossed once to NOD/LtJ prior to intercrossing.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 001976 NOD/ShiLtJ | ||
| Considerations for Choosing Controls | ||
Additional Web Information
Genetic Quality Control Annual Report
Phenotype
Phenotype Information
View Research Applications
Research Applications
This mouse can be used to support research in many areas including:
Diabetes and Obesity Research
Type 1 Diabetes (IDDM)
Immunology and Inflammation Research
Autoimmunity
Type 1 Diabetes
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Ica1tm1Mdos | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, H Michael Dosch | ||
| Allele Type | Targeted (knock-out) | ||
| Common Name(s) | ICA69null; | ||
| Mutation Made By | H. Dosch, University of Toronto | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Gene Symbol and Name | Ica1, islet cell autoantigen 1 | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | 69kDa; ICA69; ICAp69; | ||
| Molecular Note | The gene was disrupted by insertion of a tTA-neo cassette into the Tep69 epitope sequence of exon 2 via homologous recombination. Absence of gene expression in homozygous mutant animals was confirmed by Western blot analysis of brain and salivary gland proteins. [MGI Ref ID J:83526] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Ica1tm1Mdos, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Winer S; Astsaturov I; Gaedigk R; Hammond-McKibben D; Pilon M; Song A; Kubiak V; Karges W; Arpaia E; McKerlie C; Zucker P; Singh B; Dosch HM. 2002. ICA69(null) nonobese diabetic mice develop diabetes, but resist disease acceleration by cyclophosphamide. J Immunol 168(1):475-82. [PubMed: 11751995] [MGI Ref ID J:83526]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.Colony Maintenance
Breeding & Husbandry Females homozygote for the targeted mutation at The Jackson Laboratory have fertility issues. Recommended breeding scheme is heterozygote x heterozygote or heterozygote female x homozygote male.
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
General Supply Notes
- This strain is included in the Type 1 Diabetes Repository collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 001976 NOD/ShiLtJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
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