Strain Name: |
NOD/ShiLt-Tg(ACTB-Ica1/EGFP)18Mdos/MdosJ |
|---|---|
Stock Number: |
005082 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Former Name |
NOD/Lt-Tg(ACTB-Ica1/EGFP)18Mdos/MdosJ (Changed: 23-FEB-07
) |
|
NOD/Lt-Tg(ACTb-Ica1/EGFP)18Mdos/MdosJ (Changed: 17-FEB-05
) | |
| Genes & Alleles | ACTB; GFP; Ica1; Tg(ACTB-Ica1/EGFP)18Mdos; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Coisogenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator H. Dosch, University of Toronto H2 Haplotype g7 Appearance
albino, pink eyed
Related Genotype: A/A Tyrc/TyrcStrain Description
ACTB-Ica1/EGFP (commonly referred to as Ica69/EGFP) Line 18 transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Tissue fluorescence is strongest in the heart, intermediate in the exocrine pancreas and kidney and weak in the liver. Tissue fluorescence is observed in isolated transgenic islets. No fluorescence was observed in the brain, thymocytes or spleen cells. NOD transgenic females exhibit reduced diabetes incidence (45%) by 40 weeks of age and more rapid insulitis onset (50%, severe infiltration) at 12 weeks of age compared with NOD controls (80% diabetic by 40 wk and 20% insulitis-positive at 12 weeks). Intra-islet lymphocytes expressing interferon gamma were 50 times more frequent in sections from transgenic Line 18 than in wild-type controls, while IL4 stained cells were barely detectable in either.NOD-Tg(ACTB-Ica1/EGFP)18Mdos/MdosJ can be used as a tool to study the constant over expression of Ica1 in NOD mice and to understand tissue selectively in activating T cells recognizing a widely-expressed antigen.
Strain Development
The fusion transgene ACTB-Ica1/EGFP is under the control of the chicken beta actin (ACTb) promoter and encodes an islet cell autoantigen 1 (Ica1) molecule in which a full-length green florescent protein (EGFP) replaces the Ica1 3' stop codon. This transgenic construct was injected into NOD/Lt oocytes and the resulting founders were backcrossed to NOD/Lt (A. Song and H.M. Dosch et al, unpublished). In 2004, transgenic line 18 arrived at The Jackson Laboratory, was backcrossed to NOD/LtJ one generation and is maintained by hemizygote x +/+ matings.
Gene & Allele Details
| Allele Symbol | Tg(ACTB-Ica1/EGFP)18Mdos | ||
|---|---|---|---|
| Allele Name | transgene insertion 18, H Michael Dosch | ||
| Mutation Made By | H. Dosch, University of Toronto | ||
| Strain of Origin | NOD/ShiLt | ||
| Site of Expression | Strongest in the heart, intermediate in the exocrine pancreas and kidney and weak in the liver. Tissue fluorescence is observed in isolated transgenic islets. No fluorescence was observed in the brain, thymocytes or spleen cells. | ||
| Expressed Gene | Ica1, islet cell autoantigen 1, mouse, laboratory | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | ACTB, actin, beta, chicken | ||
| General Note | Line 18 transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. NOD transgenic females exhibit reduced diabetes incidence (45%) by 40 weeks of age and more rapid insulitis onset (50%, severe infiltration) at 12 weeks of age compared with NOD controls (80% diabetic by 40 wk and 20% insulitis-positive at 12 weeks). Intra-islet lymphocytes expressing interferon gamma were 50 times more frequent in sections from transgenic Line 18 than in wild-type controls, while IL4 stained cells were barely detectable in either. | ||
| Molecular Note | A chicken beta actin promoter drives the expression of the fusion gene Ica1/EGFP, which encodes an islet cell autoantigen 1 (Ica1) molecule in which a full-length enhanced green florescent protein (EGFP) replaces the Ica1 natural 3'-stop codon. Tissue fluorescence is strongest in the heart, intermediate in the exocrine pancreas and kidney and weak in the liver. Tissue fluorescence is observed in isolated transgenic islets. No fluorescence was observed in the brain, thymocytes or spleen cells. | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001976 NOD/ShiLtJ | ||
| Considerations for Choosing Controls | ||
Colony Maintenance
| Diet Information | LabDiet® 5K52/5K67 |
|---|
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005080 NOD.129-Ica1tm1Mdos/MdosJ
Additional Web Information
Fluorescent Proteins/lacZ Systems
Research Applications
This mouse can be used to support research in many areas including:
GFP relatedDiabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains (NOD Transgenics)
Immunology and Inflammation Research
Autoimmunity (Type 1 Diabetes)
Research Tools
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers: pancreatic beta cells)
Research Tools
Fluorescent Proteins
References
Additional ReferencesPrice and Supply Information
| Strain Name: | NOD/ShiLt-Tg(ACTB-Ica1/EGFP)18Mdos/MdosJ |
| Stock Number: | 005082 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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