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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Generation F?+15N5F2 (05-DEC-07) Donating Investigator IMR Colony, The Jackson Laboratory Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA or protein) is detected. Although lymphocyte development appears unremarkable, irregularities are observed in lymphocyte trafficking. Tail-injected lymphocytes derived from null animals exhibit an impaired ability to traffic to peripheral lymph nodes, and to a much greater degree, the thymus. Transcription and translation of the targeted allele subsequently lead to the synthesis of the lacZ protein under control of the 5' regulatory elements of the endogenous locus in all cells and tissues normally expressing one or several of the CD44 isoforms.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Development
A targeting vector containing a neomycin resistance/lacZ cassette was used to disrupt exon1 and a portion of intron 1 resulting in total loss of Cd44 transcription. Endogenous Cd44 regulatory elements direct the transcription of the inserted neo/lacZ cassette. The construct was electroporated into 129-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were backcrossed to C57BL/6. After arrival at The Jackson Laboratory (as Stock No. 003899), SNP analysis suggested that the strain had at least one other unidentified strain in its genetic background. These mixed background were mice were then backcrossed to C57BL/6J inbred mice for at least 5 generations to generate this congenic strain (Stock No. 005085).
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (176 strains)
005878 NOD.Cg-Cd44tm1Hbg/J 003899 STOCK Cd44tm1Hbg/J
008008 STOCK Cd44tm1Ugu/J
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Cd44tm1Hbg/Cd44tm1Hbg
involves: 129S1/Sv * 129X1/SvJ * C57BL/6
- immune system phenotype
- abnormal CD4-positive T cell morphology (MGI Ref ID J:68069)
- CD4+ T cells do not have a CD25low+ population
- abnormal CD8-positive T cell morphology (MGI Ref ID J:68069)
- CD8+ T cells do not have a CD25low+ population
- abnormal leukocyte migration/homing (MGI Ref ID J:68069)
- tail injected lymphocytes derived from homozygotes enter thymus 10-20 times less efficiently than controls
- migration to peripheral lymph nodes is delayed initially, but is balanced out in 24 hours
- lymphocyte migration to spleen is similar in mutant and control
- hematopoietic system phenotype
- abnormal CD4-positive T cell morphology (MGI Ref ID J:68069)
- CD4+ T cells do not have a CD25low+ population
- abnormal CD8-positive T cell morphology (MGI Ref ID J:68069)
- CD8+ T cells do not have a CD25low+ population
This mouse can be used to support research in many areas including:
Cd44tm1Hbg relatedDevelopmental Biology Research
Internal/Organ Defects (Lymphoid Tissue Defects)
Lymphoid Tissue Defects
Immunology and Inflammation Research
Lymphoid Tissue Defects (Lymphocyte Homing)
Internal/Organ Research
Lymphoid Tissue Defects
Research Tools
lacZ Expression
Cancer Research
Other (tumor metastasis)
Cell Biology Research
Cell Motility Defects
Defects in Cell Adhesion Molecules
Developmental Biology Research
Defects in Cell Adhesion Molecules
Hematological Research
Immunological Defects
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Inflammation
Lymphoid Tissue Defects (Lymphocyte Homing)
Research Tools
Cancer Research (tumor immunology)
Immunology and Inflammation Research
Internal/Organ Research
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Cd44tm1Hbg | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Frank Hilberg | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | CD44-; | ||
| Mutation Made By | Frank Hilberg, Boehringer Ingelheim R&D Vienna | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | lacZ expression is detected in all cells and tissues that normally express one or several CD44 isoforms. | ||
| Gene Symbol and Name | Cd44, CD44 antigen | ||
| Chromosome | 2 | ||
| Gene Common Name(s) | AU023126; AW121933; AW146109; CD44A; CDW44; CSPG8; ECMR-III; HCELL; HERMES; IN; LHR; Ly-24; MC56; MDU2; MDU3; METAA; MGC10468; MGC124941; MIC4; MUTCH-I; Pgp-1; Pgp1; RHAMM; expressed sequence AU023126; expressed sequence AW121933; expressed sequence AW146109; lymphocyte antigen 24; phagocyte glycoprotein 1; | ||
| Molecular Note | A promoterless lacZ gene followed by a neomycin selection cassette was fused in frame directly following the translation start codon. Northern blot analysis on RNA derived from E13.5 homozygous embryos demonstrated that no wild-type transcript was detectable. Flow cytometry analysis on cells derived from thymus, spleen and lymph nodes of homozygous mice confirmed that no stable encoded protein was expressed on the cell surface. [MGI Ref ID J:68069] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Cd44tm1Hbg, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Protin U; Schweighoffer T; Jochum W; Hilberg F. 1999. CD44-deficient mice develop normally with changes in subpopulations and recirculation of lymphocyte subsets. J Immunol 163(9):4917-23. [PubMed: 10528194] [MGI Ref ID J:68069]
Additional References
Cd44tm1Hbg relatedAssimakopoulos D; Kolettas E; Patrikakos G; Evangelou A. 2002. The role of CD44 in the development and prognosis of head and neck squamous cell carcinomas. Histol Histopathol 17(4):1269-81. [PubMed: 12371152] [MGI Ref ID J:79681]
Bonder CS; Clark SR; Norman MU; Johnson P; Kubes P. 2006. Use of CD44 by CD4+ Th1 and Th2 lymphocytes to roll and adhere. Blood 107(12):4798-806. [PubMed: 16497973] [MGI Ref ID J:132863]
Bourguignon LY; Ramez M; Gilad E; Singleton PA; Man MQ; Crumrine DA; Elias PM; Feingold KR. 2006. Hyaluronan-CD44 interaction stimulates keratinocyte differentiation, lamellar body formation/secretion, and permeability barrier homeostasis. J Invest Dermatol 126(6):1356-65. [PubMed: 16557236] [MGI Ref ID J:108938]
Bradl H; Schuh W; Jack HM. 2004. CD44 is dispensable for B lymphopoiesis. Immunol Lett 95(1):71-5. [PubMed: 15325800] [MGI Ref ID J:110964]
Cao JJ; Singleton PA; Majumdar S; Boudignon B; Burghardt A; Kurimoto P; Wronski TJ; Bourguignon LY; Halloran BP. 2005. Hyaluronan increases RANKL expression in bone marrow stromal cells through CD44. J Bone Miner Res 20(1):30-40. [PubMed: 15619667] [MGI Ref ID J:101563]
Hayer S; Steiner G; Gortz B; Reiter E; Tohidast-Akrad M; Amling M; Hoffmann O; Redlich K; Zwerina J; Skriner K; Hilberg F; Wagner EF; Smolen JS; Schett G. 2005. CD44 is a determinant of inflammatory bone loss. J Exp Med 201(6):903-14. [PubMed: 15781582] [MGI Ref ID J:97992]
Hidalgo A; Peired AJ; Wild MK; Vestweber D; Frenette PS. 2007. Complete identification of E-selectin ligands on neutrophils reveals distinct functions of PSGL-1, ESL-1, and CD44. Immunity 26(4):477-89. [PubMed: 17442598] [MGI Ref ID J:123577]
Ho J; Kurtz CC; Naganuma M; Ernst PB; Cominelli F; Rivera-Nieves J. 2008. A CD8+/CD103high T cell subset regulates TNF-mediated chronic murine ileitis. J Immunol 180(4):2573-80. [PubMed: 18250468] [MGI Ref ID J:131978]
Huebener P; Abou-Khamis T; Zymek P; Bujak M; Ying X; Chatila K; Haudek S; Thakker G; Frangogiannis NG. 2008. CD44 is critically involved in infarct healing by regulating the inflammatory and fibrotic response. J Immunol 180(4):2625-33. [PubMed: 18250474] [MGI Ref ID J:131974]
Kawana H; Karaki H; Higashi M; Miyazaki M; Hilberg F; Kitagawa M; Harigaya K. 2008. CD44 Suppresses TLR-Mediated Inflammation. J Immunol 180(6):4235-45. [PubMed: 18322236] [MGI Ref ID J:132955]
Khan AI; Kerfoot SM; Heit B; Liu L; Andonegui G; Ruffell B; Johnson P; Kubes P. 2004. Role of CD44 and hyaluronan in neutrophil recruitment. J Immunol 173(12):7594-601. [PubMed: 15585887] [MGI Ref ID J:94850]
Kipnis A; Basaraba RJ; Turner J; Orme IM. 2003. Increased neutrophil influx but no impairment of protective immunity to tuberculosis in mice lacking the CD44 molecule. J Leukoc Biol 74(6):992-7. [PubMed: 12972514] [MGI Ref ID J:86947]
Krause DS; Lazarides K; von Andrian UH; Van Etten RA. 2006. Requirement for CD44 in homing and engraftment of BCR-ABL-expressing leukemic stem cells. Nat Med 12(10):1175-80. [PubMed: 16998483] [MGI Ref ID J:115161]
Larkin J; Renukaradhya GJ; Sriram V; Du W; Gervay-Hague J; Brutkiewicz RR. 2006. CD44 differentially activates mouse NK T cells and conventional T cells. J Immunol 177(1):268-79. [PubMed: 16785522] [MGI Ref ID J:134432]
Morioka Y; Yamasaki K; Leung D; Gallo RL. 2008. Cathelicidin antimicrobial peptides inhibit hyaluronan-induced cytokine release and modulate chronic allergic dermatitis. J Immunol 181(6):3915-22. [PubMed: 18768846] [MGI Ref ID J:139105]
Taylor KR; Yamasaki K; Radek KA; Di Nardo A; Goodarzi H; Golenbock D; Beutler B; Gallo RL. 2007. Recognition of hyaluronan released in sterile injury involves a unique receptor complex dependent on Toll-like receptor 4, CD44, and MD-2. J Biol Chem 282(25):18265-75. [PubMed: 17400552] [MGI Ref ID J:123387]
Zarbock A; Lowell CA; Ley K. 2007. Spleen tyrosine kinase Syk is necessary for E-selectin-induced alpha(L)beta(2) integrin-mediated rolling on intercellular adhesion molecule-1. Immunity 26(6):773-83. [PubMed: 17543554] [MGI Ref ID J:123587]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are bred as homozygotes. Mating System Homozygote x Homozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $155.70 Female or Male Homozygous for Cd44tm1Hbg *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $311.40 Homozygous for Cd44tm1Hbg x Homozygous for Cd44tm1Hbg
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $202.50 Female or Male Homozygous for Cd44tm1Hbg *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $404.90 Homozygous for Cd44tm1Hbg x Homozygous for Cd44tm1Hbg
Additional Supply Details
| Supply Notes |
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
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Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
Contact information
General inquiries
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice & Services Conditions of Use
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