Strain Name: |
C57BL/6-Tg(Camk2a-IDE)1Selk/J |
|---|---|
Stock Number: |
005087 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | Camk2a; IDE; Tg(Camk2a-IDE)1Selk; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Strain Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator Dennis Selkoe, Brigham & Women’s Hsp, Harvard Med Schl Strain Description
These transgenic mice express human insulin-degrading enzyme (IDE) under the direction of the mouse calcium/calmodulin-dependent protein kinase II alpha (Camk2a) promoter. Transgene expression is approximately 2 fold relative to wildtype controls, as detected by quantitative Western blot analysis and protease activity assay. The levels of soluble alpha beta-amyloid X-40 and soluble alpha beta-amyloid X-42 are reduced in 2 to 3 month old transgenic mice. Mice that are hemizygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed to B6.Cg-Tg(PDGFB-APPSwInd)20Lms/2J (Stock No. 006293) mice, amyloid beta plaque burden in the resulting double transgenic mice is reduced by half. This mutant mouse strain may be useful in studies of Alzheimer's Disease, specifically the enhancement of amyloid beta-protein degradation.Strain Development
A transgenic construct containing the human insulin-degrading enzyme, IDE, gene under the control of the mouse calcium/calmodulin-dependent protein kinase II alpha (Camk2a) promoter, and SV40 polyadenylation site sequence was injected into fertilized C57BL/6 oocytes. The resulting founder animals were crossed to C57BL/6 mice, and are maintained on the C57BL/6 background.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(Camk2a-IDE)1Selk | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Dennis J Selkoe | ||
| Common Name(s) | 2xIDE; | ||
| Mutation Made By | Malcolm Leissring, Brigham & Women’s Hsp, Harvard Med Schl | ||
| Strain of Origin | C57BL/6 | ||
| Expressed Gene | IDE, insulin-degrading enzyme, human | ||
| Promoter | Camk2a, calcium/calmodulin-dependent protein kinase II alpha, mouse, laboratory | ||
| General Note |
Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. The levels of soluble alpha beta-amyloid X-40 and soluble alpha beta-amyloid X-42 are reduced in 2 to 3 month old transgenic mice. In conjunction with Tg(PDGFB-APPSwInd)20Lms, transgenic mice develop only about half the amyloid beta plaque burden displayed by transgenic mice carrying Tg(PDGFB-APPSwInd)20Lms alone. | ||
| Molecular Note | Transgenic mice express human insulin-degrading enzyme (IDE) under the direction of the mouse calcium/calmodulin-dependent protein kinase II alpha (Camk2a) promoter. Transgene expression is approximately 2 fold relative to wildtype controls as detected by quantitative Western blot analysis and protease activity assay. The levels of soluble alpha beta-amyloid X-40 and soluble alpha beta-amyloid X-42 are reduced in 2 to 3 month old transgenic mice. [MGI Ref ID J:87150] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Tg(Camk2a-IDE)1Selk
Colony Maintenance
| Breeding & Husbandry | The strain is maintained as a hemizygote. The Donating Investigator reports reproductive performance of this strain is poor. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Camk2a
002362 B6.129P2-Camk2atm1Sva/J 007574 B6.Cg-Tg(Camk2a-Crebbp*)1364Tabe/J 005855 B6.Cg-Tg(Camk2a-Prkaca)426Tabe/J 005359 B6.Cg-Tg(Camk2a-cre)T29-1Stl/J 007004 B6.Cg-Tg(Camk2a-tTA)1Mmay/DboJ 003010 B6;CBA-Tg(Camk2a-tTA)1Mmay/J 004995 C3H-Tg(Camk2a-Creb1/ESR1)3Sva/J 006575 C57BL/6-Camk2atm1Vyb/J 006579 C57BL/6-Tg(Camk2a-Bdnf)A9Stl/J 005086 C57BL/6-Tg(Camk2a-MME)3Selk/J View Strains carrying other alleles of Camk2a (10 strains)
Additional Web Information
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.
Research Applications
This mouse can be used to support research in many areas including:
Neurobiology Research
Alzheimer's Disease
References
Selected Reference(s)
Leissring MA; Farris W; Chang AY; Walsh DM; Wu X; Sun X; Frosch MP; Selkoe DJ. 2003. Enhanced proteolysis of beta-amyloid in APP transgenic mice prevents plaque formation, secondary pathology, and premature death. Neuron 40(6):1087-93. [PubMed: 14687544] [MGI Ref ID J:87150]
Price and Supply Information
| Strain Name: | C57BL/6-Tg(Camk2a-IDE)1Selk/J |
| Stock Number: | 005087 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
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- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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