Strain Name: |
129S-Pnpla6tm1Blw/J |
|---|---|
Stock Number: |
005091 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Former Name |
129S-Ntetm1Blw/J (Changed: 19-JUL-06
) |
| Genes & Alleles | Pnpla6; Pnpla6tm1Blw; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Strain Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse Donating Investigator Carrolee Barlow, Brain Cells Inc Strain Description
Homozygous null mice have an embryonic lethal phenotype, failing to develop past embryonic day 8.5. Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and are more active than wildtype littermates. A reduced level of protein gene product is detected by immunoprecipitation and Western blot analysis of brain, testes and kidney, but protein levels are not reduced in liver. Beta galactosidase activity in heterozygotes aged embryonic day 13.5 is found in the developing lens and spinal cord. In adult heterozygotes, beta galactosidase activity is detected throughout the brain, especially in the cerebellar Purkinje cells and hippocampus. Beta galactosidase activity patterns mimic the endogenous gene expression pattern. NTE activity in brain tissue is reduced by 40%. Heterozygotes are more sensitive to organophosphate toxicity with increased motor activity and mortality. This mutant mouse strain may be useful in studies of neuropathological hyperactivity, Gulf War Syndrome, and organophosphate-induced sub-acute neurotoxicity.Strain Development
A targeting vector containing lacZ, neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exons 4 to 10. The lacZ coding sequence was inserted into exon 4, and exons 5 to 10 were deleted. The construct was electroporated into 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals from two independent ES cell clones were crossed. The offspring were mated to 129S1/SvImJ (STOCK#2448) mice.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Pnpla6tm1Blw | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Carrolee Barlow | ||
| Common Name(s) | Nte-/-; | ||
| Mutation Made By | Christopher Winrow, Salk Institute for Biological Studies | ||
| Strain of Origin | 129S6/SvEvTac | ||
| ES Cell Line Name | TC-1 | ||
| ES Cell Line Strain | 129S6/SvEvTac | ||
| Site of Expression | lacZ espression in heterozygotes at embryonic day 13.5 is found in the developing lens and spinal chord. In adults, beta-galgalactosidase is detected throughout the brain, especially in the cerebellar Purkinje cells and the hippocampus. | ||
| Gene Symbol and Name | Pnpla6, patatin-like phospholipase domain containing 6 | ||
| Chromosome | 8 | ||
| Gene Common Name(s) | AI661849; MSws; NTE; NTEMND; Nte; RGD1564611; SPG39; Swiss-cheese; expressed sequence AI661849; neuropathy target esterase; sws; | ||
| Molecular Note | The gene was disrupted by an in-frame fusion of lacZ to exon 4 and replacement of exons 5-10 with a neomycin resistance gene. [MGI Ref ID J:82791] | ||
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Nte tm1Blw
Colony Maintenance
| Breeding & Husbandry | This strain is maintained as a heterozygote due to homozygous embryonic lethality. Heterozygotes are more active than wildtype littermates. |
|---|
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (174 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Genetic Quality Control Annual Report
Research Applications
This mouse can be used to support research in many areas including:
Neurobiology Research
Behavioral and Learning Defects
Research Tools
lacZ Expression
Toxicology Research
References
Selected Reference(s)
Additional ReferencesWinrow CJ; Hemming ML; Allen DM; Quistad GB; Casida JE; Barlow C. 2003. Loss of neuropathy target esterase in mice links organophosphate exposure to hyperactivity. Nat Genet 33(4):477-85. [PubMed: 12640454] [MGI Ref ID J:82791]
Price and Supply Information
| Strain Name: | 129S-Pnpla6tm1Blw/J |
| Stock Number: | 005091 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to the Supply Notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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