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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
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Description
Strain Information
Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Hemizygote x +/+ sibling (Female x Male) 13-MAY-08 Species laboratory mouse Generation F?+9 (31-DEC-08) Donating Investigator Elaine Fuchs, The Rockefeller University Important Note
This strain is segregating for Gnat2cpfl3, cone photoreceptor function loss 3, which affects bright light (photopic) vision.Description
These transgenic mice express the human histone 1, H2bj, protein (HIST1H2BJ) and Green Fluorescent Protein (GFP) fusion protein, HIST1H2BJ/GFP, under the control of a tetracycline-responsive promoter element (TRE; tetO). Transgenic expression occurs in widespread fashion. When hemizygotes are bred with another transgenic mouse expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA) to create bitransgenic animals, tissue-specific HIST1H2BJ/GFP transgene expression can be regulated with the tetracycline analog, doxycycline. Pulse-chase administration of doxycycline results in retention of signal in rarely dividing, or infrequently cycling, label-retaining cells (LRCs). Potential constitutive transgene expression should be examined in tissues of interest.Development
A transgenic construct containing sequences encoding the human histone 1, H2bj, protein (HIST1H2BJ) and enhanced Green Fluorescent Protein (GFP) fusion protein, HIST1H2BJ/GFP, under the control of a tetracycline-responsive promoter element (TRE) and cytomegalovirus minimal promoter (mCMV), was introduced into CD-1 donor eggs. Founder line Male 47 was subsequently established.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (225 strains)
003072 ALS/LtJ 006180 CD10/JlsJ 005052 PN/nBSwUmabJ 002746 SENCARA/PtJ 002747 SENCARB/PtJ 002748 SENCARC/PtJ 006135 STOCK Sgk3fz-ica/McirJ 003773 STOCK Tg(CAG-ECFP)CK6Nagy/J 005645 STOCK Tg(CAG-mRFP1)1F1Hadj/J 004623 STOCK Tg(Fos-lacZ)34Efu/J 005667 STOCK Tg(Neurog3-cre)C1Able/J 003262 STOCK Tg(Trp53A135V)L3Ber/J 005699 STOCK Tg(tetO-Ipf1,EGFP)956.6Macd/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Genetic Quality Control Annual Report
Tet Expression Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
GFP relatedCell Biology Research
Cell Cycle Regulation
Transcriptional Regulation
Research Tools
Cell Biology Research
Fluorescent Proteins
Tet Expression Systems
tTA/rtTA Responsive Strains
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(tetO-HIST1H2BJ/GFP)47Efu | ||
|---|---|---|---|
| Allele Name | transgene insertion 47, Elaine Fuchs | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | K14-H2B-GFP; K14H2BGFP; pTRE-H2BGFP; tetO-H2bGFP; | ||
| Mutation Made By | Tudorita Tumbar, | ||
| Strain of Origin | CD-1 | ||
| Site of Expression | These mice express the human histone 1, H2BJ, protein (HIST1H2BJ) and Green Fluorescent Protein (GFP) fusion protein, HIST1H2BJ/GFP, under the control of a tetracycline-responsive promoter element (TRE;tetO). When crossed with mice expressing a tetracycline-transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), tissue specific transgene expression in the bitransgenic progeny can be regulated by the administration of the tetracycline analog, doxycycline. Potential constitutive transgene expression should be examined in tissues of interest. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Expressed Gene | HIST1H2BJ, histone cluster 1, H2bj, human | ||
| Promoter | tetO, tet operator, | ||
| Molecular Note | This transgene contains sequence encoding the human histone 1, H2bj, protein (HIST1H2BJ) and green fluorescent protein fusion protein, HIST1H2BJ/GFP, under the control of a tetracycline-responsive promoter element (TRE) and cytomegalovirus regulatory elements (mCMV). [MGI Ref ID J:90563] | ||
| Allele Symbol | Gnat2cpfl3 | ||
| Allele Name | cone photoreceptor function loss 3 | ||
| Allele Type | Spontaneous | ||
| Common Name(s) | Gnat2; | ||
| Strain of Origin | various | ||
| Gene Symbol and Name | Gnat2, guanine nucleotide binding protein, alpha transducing 2 | ||
| Chromosome | 3 | ||
| Gene Common Name(s) | ACHM4; AW490837; GNATC; Gnat-2; Gt-2; Tcalpha; expressed sequence AW490837; | ||
| General Note |
This allele has been detected in the following strains either by genotyping or complementation testing: ALS/LtJ, SENCARA/PtJ, SENCARB/PtJ, SENCARC/PtJ, PN/nBSwUmabJ. (J:122428) Phenotypic Similarity to Human Syndrome in Orthologous Human Gene: OMIM +139340 ACHROMATOPSIA 4. | ||
| Molecular Note | A single nucleotide substitution of G to A at position 598 in exon 6. This mutation converts codon 200 from apartic acid to asparagine. [MGI Ref ID J:122428] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), Melt Curve Analysis
Fluorescent Proteins (Generic GFP), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Tumbar T; Guasch G; Greco V; Blanpain C; Lowry WE; Rendl M; Fuchs E. 2004. Defining the epithelial stem cell niche in skin. Science 303(5656):359-63. [PubMed: 14671312] [MGI Ref ID J:90563]
Additional References
Chang B; Dacey MS; Hawes NL; Hitchcock PF; Milam AH; Atmaca-Sonmez P; Nusinowitz S; Heckenlively JR. 2006. Cone photoreceptor function loss-3, a novel mouse model of achromatopsia due to a mutation in Gnat2. Invest Ophthalmol Vis Sci 47(11):5017-21. [PubMed: 17065522] [MGI Ref ID J:122428]
Gnat2cpfl3 relatedTg(tetO-HIST1H2BJ/GFP)47Efu relatedAlexander JJ; Umino Y; Everhart D; Chang B; Min SH; Li Q; Timmers AM; Hawes NL; Pang JJ; Barlow RB; Hauswirth WW. 2007. Restoration of cone vision in a mouse model of achromatopsia. Nat Med 13(6):685-7. [PubMed: 17515894] [MGI Ref ID J:121897]
Chang B; Dacey MS; Hawes NL; Hitchcock PF; Milam AH; Atmaca-Sonmez P; Nusinowitz S; Heckenlively JR. 2006. Cone photoreceptor function loss-3, a novel mouse model of achromatopsia due to a mutation in Gnat2. Invest Ophthalmol Vis Sci 47(11):5017-21. [PubMed: 17065522] [MGI Ref ID J:122428]
Deng WT; Sakurai K; Liu J; Dinculescu A; Li J; Pang J; Min SH; Chiodo VA; Boye SL; Chang B; Kefalov VJ; Hauswirth WW. 2009. Functional interchangeability of rod and cone transducin alpha-subunits. Proc Natl Acad Sci U S A 106(42):17681-6. [PubMed: 19815523] [MGI Ref ID J:153749]
Nusinowitz S; Ridder WH rd; Ramirez J. 2007. Temporal response properties of the primary and secondary rod-signaling pathways in normal and Gnat2 mutant mice. Exp Eye Res 84(6):1104-14. [PubMed: 17408617] [MGI Ref ID J:126462]
Umino Y; Solessio E; Barlow RB. 2008. Speed, spatial, and temporal tuning of rod and cone vision in mouse. J Neurosci 28(1):189-98. [PubMed: 18171936] [MGI Ref ID J:131050]
Brennand K; Huangfu D; Melton D. 2007. All beta Cells Contribute Equally to Islet Growth and Maintenance. PLoS Biol 5(7):e163. [PubMed: 17535113] [MGI Ref ID J:124045]
Creamer BA; Triplett AA; Wagner KU. 2009. Longitudinal analysis of mammogenesis using a novel tetracycline-inducible mouse model and in vivo imaging. Genesis 47(4):234-45. [PubMed: 19208431] [MGI Ref ID J:148684]
Devenport D; Fuchs E. 2008. Planar polarization in embryonic epidermis orchestrates global asymmetric morphogenesis of hair follicles. Nat Cell Biol 10(11):1257-68. [PubMed: 18849982] [MGI Ref ID J:145627]
Greco V; Chen T; Rendl M; Schober M; Pasolli HA; Stokes N; Dela Cruz-Racelis J; Fuchs E. 2009. A two-step mechanism for stem cell activation during hair regeneration. Cell Stem Cell 4(2):155-69. [PubMed: 19200804] [MGI Ref ID J:149690]
Wilson A; Laurenti E; Oser G; van der Wath RC; Blanco-Bose W; Jaworski M; Offner S; Dunant CF; Eshkind L; Bockamp E; Lio P; Macdonald HR; Trumpp A. 2008. Hematopoietic stem cells reversibly switch from dormancy to self-renewal during homeostasis and repair. Cell 135(6):1118-29. [PubMed: 19062086] [MGI Ref ID J:147599]
Zhang YV; Cheong J; Ciapurin N; McDermitt DJ; Tumbar T. 2009. Distinct self-renewal and differentiation phases in the niche of infrequently dividing hair follicle stem cells. Cell Stem Cell 5(3):267-78. [PubMed: 19664980] [MGI Ref ID J:154136]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are maintained as hemizygote. Mating System Hemizygote x +/+ sibling (Female x Male) 13-MAY-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Hemizygous for Tg(tetO-HIST1H2BJ/GFP)47Efu
Pairs /Price (US dollars $) Pair Genotype $297.85 Hemizygous for Tg(tetO-HIST1H2BJ/GFP)47Efu x Noncarrier $297.85 Noncarrier x Hemizygous for Tg(tetO-HIST1H2BJ/GFP)47Efu
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Hemizygous for Tg(tetO-HIST1H2BJ/GFP)47Efu
Pairs /Price (US dollars $) Pair Genotype $387.30 Hemizygous for Tg(tetO-HIST1H2BJ/GFP)47Efu x Noncarrier $387.30 Noncarrier x Hemizygous for Tg(tetO-HIST1H2BJ/GFP)47Efu
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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| Important Note | |
| This strain is segregating for Gnat2cpfl3, cone photoreceptor function loss 3, which affects bright light (photopic) vision. | |
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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