Strain Name: |
STOCK Tg(tetO-HIST1H2BJ/GFP)47Efu/J |
|---|---|
Stock Number: |
005104 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | GFP; Gnat2; Gnat2cpfl3; HIST1H2BJ; Tg(tetO-HIST1H2BJ/GFP)47Efu; tetO; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Mating System Hemizygote x +/+ sibling (Female x Male) Species laboratory mouse Donating Investigator Elaine Fuchs, The Rockefeller University Generation F?+7 (31-DEC-07) Important Note
This strain is homozygous for Gnat2cpfl3, cone photoreceptor function loss 3, which affects bright light (photopic) vision.Strain Description
These transgenic mice express the human histone 1, H2bj, protein (HIST1H2BJ) and Green Fluorescent Protein (GFP) fusion protein, HIST1H2BJ/GFP, under the control of a tetracycline-responsive promoter element (TRE; tetO). Transgenic expression occurs in widespread fashion. When hemizygotes are bred with another transgenic mouse expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA) to create bitransgenic animals, tissue-specific HIST1H2BJ/GFP transgene expression can be regulated with the tetracycline analog, doxycycline. Pulse-chase administration of doxycycline results in retention of signal in rarely dividing, or infrequently cycling, label-retaining cells (LRCs). Potential constitutive transgene expression should be examined in tissues of interest.Strain Development
A transgenic construct containing sequences encoding the human histone 1, H2bj, protein (HIST1H2BJ) and enhanced Green Fluorescent Protein (GFP) fusion protein, HIST1H2BJ/GFP, under the control of a tetracycline-responsive promoter element (TRE) and cytomegalovirus minimal promoter (mCMV), was introduced into CD-1 donor eggs. Founder line Male 47 was subsequently established.
Gene & Allele Details
| Allele Symbol | Tg(tetO-HIST1H2BJ/GFP)47Efu | ||
|---|---|---|---|
| Allele Name | transgene insertion 47, Elaine Fuchs | ||
| Common Name(s) | pTRE-H2BGFP; tetO-H2bGFP; | ||
| Mutation Made By | Tudorita Tumbar, | ||
| Strain of Origin | CD-1 | ||
| Site of Expression | These mice express the human histone 1, H2BJ, protein (HIST1H2BJ) and Green Fluorescent Protein (GFP) fusion protein, HIST1H2BJ/GFP, under the control of a tetracycline-responsive promoter element (TRE;tetO). When crossed with mice expressing a tetracycline-transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), tissue specific transgene expression in the bitransgenic progeny can be regulated by the administration of the tetracycline analog, doxycycline. Potential constitutive transgene expression should be examined in tissues of interest. | ||
| Expressed Gene | HIST1H2BJ, histone cluster 1, H2bj, human | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | tetO, tet operator, | ||
| Molecular Note | This transgene contains sequence encoding the human histone 1, H2bj, protein (HIST1H2BJ) and green fluorescent protein fusion protein, HIST1H2BJ/GFP, under the control of a tetracycline-responsive promoter element (TRE) and cytomegalovirus regulatory elements (mCMV). [MGI Ref ID J:90563] | ||
| Allele Symbol | Gnat2cpfl3 | ||
| Allele Name | cone photoreceptor function loss 3 | ||
| Common Name(s) | Gnat2; | ||
| Strain of Origin | various | ||
| Gene Symbol and Name | Gnat2, guanine nucleotide binding protein, alpha transducing 2 | ||
| Chromosome | 3 | ||
| Gene Common Name(s) | ACHM4; AW490837; GNATC; Gnat-2; Gt-2; Tcalpha; expressed sequence AW490837; | ||
| General Note |
This allele has been detected in the following strains either by genotyping or complementation testing: ALS/LtJ, SENCARA/PtJ, SENCARB/PtJ, SENCARC/PtJ, PN/nBSwUmabJ. (J:122428) Phenotypic Similarity to Human Syndrome in Orthologous Human Gene: OMIM +139340 ACHROMATOPSIA 4. | ||
| Molecular Note | A single nucleotide substitution of G to A at position 598 in exon 6. This mutation converts codon 200 from apartic acid to asparagine. [MGI Ref ID J:122428] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Fluorescent Proteins (Generic GFP)
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice are maintained as hemizygote. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
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Fluorescent Protein Strains
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002448 129S1/SvImJ 003072 ALS/LtJ 006180 CD10/JlsJ 005052 PN/nBSwUmabJ 002746 SENCARA/PtJ 002747 SENCARB/PtJ 002748 SENCARC/PtJ 006135 STOCK Sgk3fz-ica/McirJ 003773 STOCK Tg(CAG-ECFP)CK6Nagy/J 005645 STOCK Tg(CAG-mRFP1)1F1Hadj/J 004623 STOCK Tg(Fos-lacZ)34Efu/J 005667 STOCK Tg(Neurog3-cre)C1Able/J 003262 STOCK Tg(Trp53A135V)L3Ber/J 005699 STOCK Tg(tetO-Ipf1,EGFP)956.6Macd/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Genetic Quality Control Annual Report
Tet Expression Systems
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
GFP relatedCell Biology Research
Cell Cycle Regulation
Transcriptional Regulation
Research Tools
Cell Biology Research
Fluorescent Proteins
Tet Expression Systems (tTA/rtTA Responsive Strains)
Research Tools
Fluorescent Proteins
References
Selected Reference(s)
Additional ReferencesTumbar T; Guasch G; Greco V; Blanpain C; Lowry WE; Rendl M; Fuchs E. 2004. Defining the epithelial stem cell niche in skin. Science 303(5656):359-63. [PubMed: 14671312] [MGI Ref ID J:90563]
Price and Supply Information
| Strain Name: | STOCK Tg(tetO-HIST1H2BJ/GFP)47Efu/J |
| Stock Number: | 005104 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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