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Description
Strain Information
Former Names STOCK Tg(Chx10-GFP/cre, ALPP)2Clc/J (Changed: 15-DEC-04 ) Type Mutant Stock; Transgenic; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Donating Investigator Connie Cepko, Harvard Medical School, HHMI Description
Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The transgene insert contains a fusion product involving Cre recombinase and an Enhanced Green Fluorescent Protein (EGFP) and a fusion product involving an internal ribosome entry site/human placental alkaline phosphatase under the control of the mouse Chx10, C. elegans ceh-10 homeo domain containing homolog promoter. This strain serves as a multifunctional reporter strain. Expression of the Chx10 BAC, as detected by in situ hybridization, mimics the endogenous Chx10 gene expression pattern. Alkaline phosphatase expression is mosaic, but specific to the retina and Muller glial cells. EGFP expression is detected in the outer neuroblastic layer of the retina at embryonic days 14.5, 17.5 and neonates. When crossed to a cre reporter strain, the resulting mice exhibit mosaicism in reporter gene expression. This mutant mouse strain may be useful in studies of retinal progenitor and bipolar cell lineage or fate mapping.Development
A transgenic construct encoding an EGFP/cre fusion protein, an internal ribosome entry site/human placental alkaline phosphatase fusion protein and an FRT-flanked selection cassette (composed of neomycin resistance and kanamycin resistance genes under the control of phosphoglycerate kinase and Tn5 transposon promoter), was inserted into a BAC encoding the mouse Chx10, C. elegans ceh-10 homeo domain containing homolog promoter. This modified BAC transgene was microinjected into B6;SJL fertilized eggs. The founder animals were bred to FVB. The mice were crossed to 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J mice (see Stock No. 003946) to remove the FRT-flanked selection cassette, and then later crossed to C57BL/6 for one generation.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (170 strains)
Additional Web Information
Cre-lox Systems
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(Chx10-EGFP/cre-ALPP)2Clc/0
involves: 129S4/SvJaeSor * C57BL/6 * SJL * FVB
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:91498)
- mice hemizygous for the transgene are viable and exhibit no gross physical or behavioral abnormalities
This mouse can be used to support research in many areas including:
GFP relatedNeurobiology Research
Fluorescent protein expression in neural tissue
Research Tools
Cre-lox System (Cre Recombinase Expression)
Fluorescent Proteins
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Genetics Research (Tissue/Cell Markers)
Genetics Research (Tissue/Cell Markers: Cre-lox System)
Neurobiology Research (cell marker)
Sensorineural Research
cre relatedResearch Tools
Fluorescent Proteins
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Chx10-EGFP/cre-ALPP)2Clc | ||
|---|---|---|---|
| Allele Name | transgene insertion 2, Constance L Cepko | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | Chx10 BAC; Chx10 BAC line 2; Chx10-cre; Tg(Chx10-GFP/cre, ALPP)2Clc/J; | ||
| Strain of Origin | C57BL/6 and SJL | ||
| Site of Expression | expression pattern is mosaic, but specific to the retina and Muller glial cells | ||
| Expressed Gene | ALPP, alkaline phosphatase, placental (Regan isozyme), human | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Vsx2, visual system homeobox 2, rat | ||
| General Note |
According to Rowan and Cepko (2004), the modified BAC DNA "was injected into male pronuclei of SJL/B6 fertilized eggs." Two transgenic lines were generated. Line 1 exhibited highly mosaic expression of the EGFP reporter. Line 2 expressed EGFP more uniformly, but weakly; excision of the FRT-neo cassette increased EGFP expression in subsequent generations, so line 2 was used for further studies. Hemizygous transgenic mice are viable, fertile, normal in size and do not display any gross physical orbehavioral abnormalities. Alkaline phosphatase expression is mosaic, but specific to the retina, and is also detected in Muller glial cells. Green Fluorescent Protein (GFP) expression is detected in the outer neuroblastic layer of the retina at embryonic days 14.5, 17.5 and neonates. When crossed to a cre reporter strain, the resulting mice exhibit mosaicism in reporter gene expression. | ||
| Molecular Note | This reporter gene expresses an enhanced green fluorescent protein-Cre recombinase fusion protein and alkaline phosphatase under control of Chx10 promoter and enhancer elements.The transgene was generated in a 129/Sv-derived bacterial artificial chromosome (BAC) containing the Chx10 gene and extending approximately 55 kb upstream of the translation start site and about 22 kb downstream of the polyadenylation signal. The 8th codon of Chx10 is joined in-frame to an EGFP-cre fusion gene followed by an internal ribosome entry sequence (IRES) coupled to the human placental alkaline phosphatase gene; 32 codons are deleted from within the N-terminal coding sequence of Chx10. The transgene was found by Southern blot analysis to be present in multiple copies, most or all of them intact. [MGI Ref ID J:91498] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Quantitative PCR, QPCR, vers. 1
Generic Cre, STD PCR, vers. 1
Tg(Chx10-EGFP/cre-ALPP)2Clc, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Rowan S; Cepko CL. 2004. Genetic analysis of the homeodomain transcription factor Chx10 in the retina using a novel multifunctional BAC transgenic mouse reporter. Dev Biol 271(2):388-402. [PubMed: 15223342] [MGI Ref ID J:91498]
Additional References
Tg(Chx10-EGFP/cre-ALPP)2Clc relatedAjioka I; Martins RA; Bayazitov IT; Donovan S; Johnson DA; Frase S; Cicero SA; Boyd K; Zakharenko SS; Dyer MA. 2007. Differentiated horizontal interneurons clonally expand to form metastatic retinoblastoma in mice. Cell 131(2):378-90. [PubMed: 17956737] [MGI Ref ID J:130181]
Chen D; Opavsky R; Pacal M; Tanimoto N; Wenzel P; Seeliger MW; Leone G; Bremner R. 2007. Rb-Mediated Neuronal Differentiation through Cell-Cycle-Independent Regulation of E2f3a. PLoS Biol 5(7):e179. [PubMed: 17608565] [MGI Ref ID J:124204]
Damiani D; Alexander JJ; O'Rourke JR; McManus M; Jadhav AP; Cepko CL; Hauswirth WW; Harfe BD; Strettoi E. 2008. Dicer inactivation leads to progressive functional and structural degeneration of the mouse retina. J Neurosci 28(19):4878-87. [PubMed: 18463241] [MGI Ref ID J:135193]
Donovan SL; Dyer MA. 2004. Developmental defects in Rb-deficient retinae. Vision Res 44(28):3323-33. [PubMed: 15536000] [MGI Ref ID J:102508]
Donovan SL; Schweers B; Martins R; Johnson D; Dyer MA. 2006. Compensation by tumor suppressor genes during retinal development in mice and humans. BMC Biol 4:14. [PubMed: 16672052] [MGI Ref ID J:110865]
Jadhav AP; Cho SH; Cepko CL. 2006. Notch activity permits retinal cells to progress through multiple progenitor states and acquire a stem cell property. Proc Natl Acad Sci U S A 103(50):18998-9003. [PubMed: 17148603] [MGI Ref ID J:118372]
Jadhav AP; Mason HA; Cepko CL. 2006. Notch 1 inhibits photoreceptor production in the developing mammalian retina. Development 133(5):913-23. [PubMed: 16452096] [MGI Ref ID J:105970]
Johnson DA; Zhang J; Frase S; Wilson M; Rodriguez-Galindo C; Dyer MA. 2007. Neuronal differentiation and synaptogenesis in retinoblastoma. Cancer Res 67(6):2701-11. [PubMed: 17363591] [MGI Ref ID J:120315]
Laurie NA; Donovan SL; Shih CS; Zhang J; Mills N; Fuller C; Teunisse A; Lam S; Ramos Y; Mohan A; Johnson D; Wilson M; Rodriguez-Galindo C; Quarto M; Francoz S; Mendrysa SM; Guy RK; Marine JC; Jochemsen AG; Dyer MA. 2006. Inactivation of the p53 pathway in retinoblastoma. Nature 444(7115):61-6. [PubMed: 17080083] [MGI Ref ID J:115580]
Livet J; Weissman TA; Kang H; Draft RW; Lu J; Bennis RA; Sanes JR; Lichtman JW. 2007. Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system. Nature 450(7166):56-62. [PubMed: 17972876] [MGI Ref ID J:125961]
Poche RA; Kwan KM; Raven MA; Furuta Y; Reese BE; Behringer RR. 2007. Lim1 is essential for the correct laminar positioning of retinal horizontal cells. J Neurosci 27(51):14099-107. [PubMed: 18094249] [MGI Ref ID J:130904]
Poche RA; Raven MA; Kwan KM; Furuta Y; Behringer RR; Reese BE. 2008. Somal positioning and dendritic growth of horizontal cells are regulated by interactions with homotypic neighbors. Eur J Neurosci 27(7):1607-14. [PubMed: 18380663] [MGI Ref ID J:133201]
Rowan S; Chen CM; Young TL; Fisher DE; Cepko CL. 2004. Transdifferentiation of the retina into pigmented cells in ocular retardation mice defines a new function of the homeodomain gene Chx10. Development 131(20):5139-52. [PubMed: 15459106] [MGI Ref ID J:93571]
Storch KF; Paz C; Signorovitch J; Raviola E; Pawlyk B; Li T; Weitz CJ. 2007. Intrinsic circadian clock of the Mammalian retina: importance for retinal processing of visual information. Cell 130(4):730-41. [PubMed: 17719549] [MGI Ref ID J:124157]
Sun H; Chang Y; Schweers B; Dyer MA; Zhang X; Hayward SW; Goodrich DW. 2006. An E2F binding-deficient Rb1 protein partially rescues developmental defects associated with Rb1 nullizygosity. Mol Cell Biol 26(4):1527-37. [PubMed: 16449662] [MGI Ref ID J:105548]
Zhang J; Gray J; Wu L; Leone G; Rowan S; Cepko CL; Zhu X; Craft CM; Dyer MA. 2004. Rb regulates proliferation and rod photoreceptor development in the mouse retina. Nat Genet 36(4):351-60. [PubMed: 14991054] [MGI Ref ID J:109491]
Zhang J; Schweers B; Dyer MA. 2004. The first knockout mouse model of retinoblastoma. Cell Cycle 3(7):952-9. [PubMed: 15190215] [MGI Ref ID J:103618]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $1900.00
Additional Supply Details
| Pricing for International shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $2470.00
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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