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- Description
- Disease & phenotype
- Genes & alleles
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Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names STOCK Tg(Chx10-GFP/cre, ALPP)2Clc/J (Changed: 15-DEC-04 ) Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Connie Cepko, Harvard Medical School, HHMI Description
Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The transgene insert contains a fusion product involving Cre recombinase and an Enhanced Green Fluorescent Protein (EGFP) and a fusion product involving an internal ribosome entry site/human placental alkaline phosphatase under the control of the mouse Chx10, C. elegans ceh-10 homeo domain containing homolog promoter. This strain serves as a multifunctional reporter strain. Expression of the Chx10 BAC, as detected by in situ hybridization, mimics the endogenous Chx10 gene expression pattern. Alkaline phosphatase expression is mosaic, but specific to the retina and Muller glial cells. EGFP expression is detected in the outer neuroblastic layer of the retina at embryonic days 14.5, 17.5 and neonates. When crossed to a cre reporter strain, the resulting mice exhibit mosaicism in reporter gene expression. This mutant mouse strain may be useful in studies of retinal progenitor and bipolar cell lineage or fate mapping.Development
A transgenic construct encoding an EGFP/cre fusion protein, an internal ribosome entry site/human placental alkaline phosphatase fusion protein and an FRT-flanked selection cassette (composed of neomycin resistance and kanamycin resistance genes under the control of phosphoglycerate kinase and Tn5 transposon promoter), was inserted into a BAC encoding the mouse Chx10, C. elegans ceh-10 homeo domain containing homolog promoter. This modified BAC transgene was microinjected into B6;SJL fertilized eggs. The founder animals were bred to FVB. The mice were crossed to 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J mice (see Stock No. 003946) to remove the FRT-flanked selection cassette, and then later crossed to C57BL/6 for one generation.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (358 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
Tg(Chx10-EGFP/cre,-ALPP)2Clc/0
involves: 129/Sv * C57BL/6 * FVB * SJL
- normal phenotype
- no abnormal phenotype detected
- mice hemizygous for the transgene are viable and exhibit no gross physical or behavioral abnormalities (MGI Ref ID J:91498)
This mouse can be used to support research in many areas including:
GFP relatedNeurobiology Research
Fluorescent protein expression in neural tissue
Research Tools
Cre-lox System
Cre Recombinase Expression
Fluorescent Proteins
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers
Tissue/Cell Markers: Cre-lox System
Neurobiology Research
cell marker
Sensorineural Research
cre relatedResearch Tools
Fluorescent Proteins
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Chx10-EGFP/cre,-ALPP)2Clc | ||
|---|---|---|---|
| Allele Name | transgene insertion 2, Constance L Cepko | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | Chx10 BAC; Chx10 BAC line 2; Chx10-cre; Chx10::Cre; Tg(Chx10-EGFP/cre-ALPP)2Clc; Tg(Chx10-GFP/cre, ALPP)2Clc/J; Tg(Vsx2-EGFP/cre-ALPP)2Clc; | ||
| Strain of Origin | C57BL/6 and SJL | ||
| Site of Expression | expression pattern is mosaic, but specific to the retina and Muller glial cells | ||
| Expressed Gene | ALPP, alkaline phosphatase, placental, human | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Vsx2, visual system homeobox 2, rat | ||
| Gene Symbol and Name | Tg(Chx10-EGFP/cre,-ALPP)2Clc, transgene insertion 2, Constance L Cepko | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Chx10 BAC; Chx10 BAC line 2; Chx10-cre; Chx10::Cre; Tg(Chx10-EGFP/cre-ALPP)2Clc; Tg(Chx10-GFP/cre, ALPP)2Clc/J; | ||
| Driver Note | Chx10 | ||
| General Note |
According to Rowan and Cepko (2004), the modified BAC DNA "was injected into male pronuclei of SJL/B6 fertilized eggs." Two transgenic lines were generated. Line 1 exhibited highly mosaic expression of the EGFP reporter. Line 2 expressed EGFP more uniformly, but weakly; excision of the FRT-neo cassette increased EGFP expression in subsequent generations, so line 2 was used for further studies. Hemizygous transgenic mice are viable, fertile, normal in size and do not display any gross physical orbehavioral abnormalities. Alkaline phosphatase expression is mosaic, but specific to the retina, and is also detected in Muller glial cells. Green Fluorescent Protein (GFP) expression is detected in the outer neuroblastic layer of the retina at embryonic days 14.5, 17.5 and neonates. When crossed to a cre reporter strain, the resulting mice exhibit mosaicism in reporter gene expression. | ||
| Molecular Note | This reporter gene expresses an enhanced green fluorescent protein-Cre recombinase fusion protein and alkaline phosphatase under control of Chx10 promoter and enhancer elements.The transgene was generated in a 129/Sv-derived bacterial artificial chromosome (BAC) containing the Chx10 gene and extending approximately 55 kb upstream of the translation start site and about 22 kb downstream of the polyadenylation signal. The 8th codon of Chx10 is joined in-frame to an EGFP-cre fusion gene followed by an internal ribosome entry sequence (IRES) coupled to the human placental alkaline phosphatase gene; 32 codons are deleted from within the N-terminal coding sequence of Chx10. The transgene was found by Southern blot analysis to be present in multiple copies, most or all of them intact. The mice were crossed to 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J mice to remove the FRT-flanked selection cassette. [MGI Ref ID J:91498] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre Quantitative PCR, QPCR
Generic Cre, Standard PCR
Tg(Chx10-EGFP/cre-ALPP)2Clc, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Rowan S; Cepko CL. 2004. Genetic analysis of the homeodomain transcription factor Chx10 in the retina using a novel multifunctional BAC transgenic mouse reporter. Dev Biol 271(2):388-402. [PubMed: 15223342] [MGI Ref ID J:91498]
Additional References
Tg(Chx10-EGFP/cre,-ALPP)2Clc relatedAjioka I; Martins RA; Bayazitov IT; Donovan S; Johnson DA; Frase S; Cicero SA; Boyd K; Zakharenko SS; Dyer MA. 2007. Differentiated horizontal interneurons clonally expand to form metastatic retinoblastoma in mice. Cell 131(2):378-90. [PubMed: 17956737] [MGI Ref ID J:130181]
Alves CH; Sanz Sanz A; Park B; Pellissier LP; Tanimoto N; Beck SC; Huber G; Murtaza M; Richard F; Sridevi Gurubaran I; Garcia Garrido M; Levelt CN; Rashbass P; Le Bivic A; Seeliger MW; Wijnholds J. 2013. Loss of CRB2 in the mouse retina mimics human retinitis pigmentosa due to mutations in the CRB1 gene. Hum Mol Genet 22(1):35-50. [PubMed: 23001562] [MGI Ref ID J:191149]
Brennan RC; Federico S; Bradley C; Zhang J; Flores-Otero J; Wilson M; Stewart C; Zhu F; Guy K; Dyer MA. 2011. Targeting the p53 Pathway in Retinoblastoma with Subconjunctival Nutlin-3a. Cancer Res 71(12):4205-13. [PubMed: 21515735] [MGI Ref ID J:173754]
Chen D; Opavsky R; Pacal M; Tanimoto N; Wenzel P; Seeliger MW; Leone G; Bremner R. 2007. Rb-Mediated Neuronal Differentiation through Cell-Cycle-Independent Regulation of E2f3a. PLoS Biol 5(7):e179. [PubMed: 17608565] [MGI Ref ID J:124204]
Chen WV; Alvarez FJ; Lefebvre JL; Friedman B; Nwakeze C; Geiman E; Smith C; Thu CA; Tapia JC; Tasic B; Sanes JR; Maniatis T. 2012. Functional significance of isoform diversification in the protocadherin gamma gene cluster. Neuron 75(3):402-9. [PubMed: 22884324] [MGI Ref ID J:188341]
Cho SH; Kim JY; Simons DL; Song JY; Le JH; Swindell EC; Jamrich M; Wu SM; Kim S. 2012. Genetic ablation of Pals1 in retinal progenitor cells models the retinal pathology of Leber congenital amaurosis. Hum Mol Genet 21(12):2663-76. [PubMed: 22398208] [MGI Ref ID J:184469]
Damiani D; Alexander JJ; O'Rourke JR; McManus M; Jadhav AP; Cepko CL; Hauswirth WW; Harfe BD; Strettoi E. 2008. Dicer inactivation leads to progressive functional and structural degeneration of the mouse retina. J Neurosci 28(19):4878-87. [PubMed: 18463241] [MGI Ref ID J:135193]
Donovan SL; Dyer MA. 2004. Developmental defects in Rb-deficient retinae. Vision Res 44(28):3323-33. [PubMed: 15536000] [MGI Ref ID J:102508]
Donovan SL; Schweers B; Martins R; Johnson D; Dyer MA. 2006. Compensation by tumor suppressor genes during retinal development in mice and humans. BMC Biol 4:14. [PubMed: 16672052] [MGI Ref ID J:110865]
Fuerst PG; Bruce F; Rounds RP; Erskine L; Burgess RW. 2012. Cell autonomy of DSCAM function in retinal development. Dev Biol 361(2):326-37. [PubMed: 22063212] [MGI Ref ID J:179393]
Hafler BP; Surzenko N; Beier KT; Punzo C; Trimarchi JM; Kong JH; Cepko CL. 2012. Transcription factor Olig2 defines subpopulations of retinal progenitor cells biased toward specific cell fates. Proc Natl Acad Sci U S A 109(20):7882-7. [PubMed: 22543161] [MGI Ref ID J:184797]
Ivanova E; Hwang GS; Pan ZH. 2010. Characterization of transgenic mouse lines expressing Cre recombinase in the retina. Neuroscience 165(1):233-43. [PubMed: 19837136] [MGI Ref ID J:158209]
Jackson CR; Ruan GX; Aseem F; Abey J; Gamble K; Stanwood G; Palmiter RD; Iuvone PM; McMahon DG. 2012. Retinal Dopamine Mediates Multiple Dimensions of Light-Adapted Vision. J Neurosci 32(27):9359-9368. [PubMed: 22764243] [MGI Ref ID J:185955]
Jadhav AP; Cho SH; Cepko CL. 2006. Notch activity permits retinal cells to progress through multiple progenitor states and acquire a stem cell property. Proc Natl Acad Sci U S A 103(50):18998-9003. [PubMed: 17148603] [MGI Ref ID J:118372]
Jadhav AP; Mason HA; Cepko CL. 2006. Notch 1 inhibits photoreceptor production in the developing mammalian retina. Development 133(5):913-23. [PubMed: 16452096] [MGI Ref ID J:105970]
Johnson DA; Zhang J; Frase S; Wilson M; Rodriguez-Galindo C; Dyer MA. 2007. Neuronal differentiation and synaptogenesis in retinoblastoma. Cancer Res 67(6):2701-11. [PubMed: 17363591] [MGI Ref ID J:120315]
Kanadia RN; Clark VE; Punzo C; Trimarchi JM; Cepko CL. 2008. Temporal requirement of the alternative-splicing factor Sfrs1 for the survival of retinal neurons. Development 135(23):3923-33. [PubMed: 18987029] [MGI Ref ID J:143205]
Kim IJ; Zhang Y; Meister M; Sanes JR. 2010. Laminar restriction of retinal ganglion cell dendrites and axons: subtype-specific developmental patterns revealed with transgenic markers. J Neurosci 30(4):1452-62. [PubMed: 20107072] [MGI Ref ID J:157687]
Lambertz I; Nittner D; Mestdagh P; Denecker G; Vandesompele J; Dyer MA; Marine JC. 2010. Monoallelic but not biallelic loss of Dicer1 promotes tumorigenesis in vivo. Cell Death Differ 17(4):633-41. [PubMed: 20019750] [MGI Ref ID J:171794]
Laurie N; Mohan A; McEvoy J; Reed D; Zhang J; Schweers B; Ajioka I; Valentine V; Johnson D; Ellison D; Dyer MA. 2009. Changes in retinoblastoma cell adhesion associated with optic nerve invasion. Mol Cell Biol 29(23):6268-82. [PubMed: 19786571] [MGI Ref ID J:154999]
Laurie NA; Donovan SL; Shih CS; Zhang J; Mills N; Fuller C; Teunisse A; Lam S; Ramos Y; Mohan A; Johnson D; Wilson M; Rodriguez-Galindo C; Quarto M; Francoz S; Mendrysa SM; Guy RK; Marine JC; Jochemsen AG; Dyer MA. 2006. Inactivation of the p53 pathway in retinoblastoma. Nature 444(7115):61-6. [PubMed: 17080083] [MGI Ref ID J:115580]
Lee SC; Cowgill EJ; Al-Nabulsi A; Quinn EJ; Evans SM; Reese BE. 2011. Homotypic regulation of neuronal morphology and connectivity in the mouse retina. J Neurosci 31(40):14126-33. [PubMed: 21976497] [MGI Ref ID J:177107]
Lefebvre JL; Kostadinov D; Chen WV; Maniatis T; Sanes JR. 2012. Protocadherins mediate dendritic self-avoidance in the mammalian nervous system. Nature 488(7412):517-21. [PubMed: 22842903] [MGI Ref ID J:186772]
Lefebvre JL; Zhang Y; Meister M; Wang X; Sanes JR. 2008. {gamma}-Protocadherins regulate neuronal survival but are dispensable for circuit formation in retina. Development 135(24):4141-51. [PubMed: 19029044] [MGI Ref ID J:142186]
Livet J; Weissman TA; Kang H; Draft RW; Lu J; Bennis RA; Sanes JR; Lichtman JW. 2007. Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system. Nature 450(7166):56-62. [PubMed: 17972876] [MGI Ref ID J:125961]
Martins RA; Davis D; Kerekes R; Zhang J; Bayazitov IT; Hiler D; Karakaya M; Frase S; Gleason S; Zakharenko SS; Johnson DA; Dyer MA. 2011. Retinoblastoma (Rb) regulates laminar dendritic arbor reorganization in retinal horizontal neurons. Proc Natl Acad Sci U S A 108(52):21111-6. [PubMed: 22160703] [MGI Ref ID J:180146]
Matsushima D; Heavner W; Pevny LH. 2011. Combinatorial regulation of optic cup progenitor cell fate by SOX2 and PAX6. Development 138(3):443-54. [PubMed: 21205789] [MGI Ref ID J:170543]
McEvoy J; Flores-Otero J; Zhang J; Nemeth K; Brennan R; Bradley C; Krafcik F; Rodriguez-Galindo C; Wilson M; Xiong S; Lozano G; Sage J; Fu L; Louhibi L; Trimarchi J; Pani A; Smeyne R; Johnson D; Dyer MA. 2011. Coexpression of normally incompatible developmental pathways in retinoblastoma genesis. Cancer Cell 20(2):260-75. [PubMed: 21840489] [MGI Ref ID J:176061]
Nittner D; Lambertz I; Clermont F; Mestdagh P; Kohler C; Nielsen SJ; Jochemsen A; Speleman F; Vandesompele J; Dyer MA; Schramm A; Schulte JH; Marine JC. 2012. Synthetic lethality between Rb, p53 and Dicer or miR-17-92 in retinal progenitors suppresses retinoblastoma formation. Nat Cell Biol 14(9):958-65. [PubMed: 22864477] [MGI Ref ID J:193515]
Oron-Karni V; Farhy C; Elgart M; Marquardt T; Remizova L; Yaron O; Xie Q; Cvekl A; Ashery-Padan R. 2008. Dual requirement for Pax6 in retinal progenitor cells. Development 135(24):4037-47. [PubMed: 19004853] [MGI Ref ID J:142509]
Park B; Alves CH; Lundvig DM; Tanimoto N; Beck SC; Huber G; Richard F; Klooster J; Andlauer TF; Swindell EC; Jamrich M; Le Bivic A; Seeliger MW; Wijnholds J. 2011. PALS1 Is Essential for Retinal Pigment Epithelium Structure and Neural Retina Stratification. J Neurosci 31(47):17230-17241. [PubMed: 22114289] [MGI Ref ID J:178056]
Poche RA; Furuta Y; Chaboissier MC; Schedl A; Behringer RR. 2008. Sox9 is expressed in mouse multipotent retinal progenitor cells and functions in Muller glial cell development. J Comp Neurol 510(3):237-50. [PubMed: 18626943] [MGI Ref ID J:187354]
Poche RA; Kwan KM; Raven MA; Furuta Y; Reese BE; Behringer RR. 2007. Lim1 is essential for the correct laminar positioning of retinal horizontal cells. J Neurosci 27(51):14099-107. [PubMed: 18094249] [MGI Ref ID J:130904]
Poche RA; Raven MA; Kwan KM; Furuta Y; Behringer RR; Reese BE. 2008. Somal positioning and dendritic growth of horizontal cells are regulated by interactions with homotypic neighbors. Eur J Neurosci 27(7):1607-14. [PubMed: 18380663] [MGI Ref ID J:133201]
Rao S; Chun C; Fan J; Kofron JM; Yang MB; Hegde RS; Ferrara N; Copenhagen DR; Lang RA. 2013. A direct and melanopsin-dependent fetal light response regulates mouse eye development. Nature 494(7436):243-6. [PubMed: 23334418] [MGI Ref ID J:194554]
Riesenberg AN; Liu Z; Kopan R; Brown NL. 2009. Rbpj cell autonomous regulation of retinal ganglion cell and cone photoreceptor fates in the mouse retina. J Neurosci 29(41):12865-77. [PubMed: 19828801] [MGI Ref ID J:154061]
Rocha SF; Lopes SS; Gossler A; Henrique D. 2009. Dll1 and Dll4 function sequentially in the retina and pV2 domain of the spinal cord to regulate neurogenesis and create cell diversity. Dev Biol 328(1):54-65. [PubMed: 19389377] [MGI Ref ID J:149456]
Rowan S; Chen CM; Young TL; Fisher DE; Cepko CL. 2004. Transdifferentiation of the retina into pigmented cells in ocular retardation mice defines a new function of the homeodomain gene Chx10. Development 131(20):5139-52. [PubMed: 15459106] [MGI Ref ID J:93571]
Roy A; de Melo J; Chaturvedi D; Thein T; Cabrera-Socorro A; Houart C; Meyer G; Blackshaw S; Tole S. 2013. LHX2 is necessary for the maintenance of optic identity and for the progression of optic morphogenesis. J Neurosci 33(16):6877-84. [PubMed: 23595746] [MGI Ref ID J:196967]
Sakagami K; Chen B; Nusinowitz S; Wu H; Yang XJ. 2012. PTEN regulates retinal interneuron morphogenesis and synaptic layer formation. Mol Cell Neurosci 49(2):171-83. [PubMed: 22155156] [MGI Ref ID J:191532]
Sakagami K; Gan L; Yang XJ. 2009. Distinct effects of Hedgehog signaling on neuronal fate specification and cell cycle progression in the embryonic mouse retina. J Neurosci 29(21):6932-44. [PubMed: 19474320] [MGI Ref ID J:149518]
Storch KF; Paz C; Signorovitch J; Raviola E; Pawlyk B; Li T; Weitz CJ. 2007. Intrinsic circadian clock of the Mammalian retina: importance for retinal processing of visual information. Cell 130(4):730-41. [PubMed: 17719549] [MGI Ref ID J:124157]
Sun H; Chang Y; Schweers B; Dyer MA; Zhang X; Hayward SW; Goodrich DW. 2006. An E2F binding-deficient Rb1 protein partially rescues developmental defects associated with Rb1 nullizygosity. Mol Cell Biol 26(4):1527-37. [PubMed: 16449662] [MGI Ref ID J:105548]
Ueki Y; Le YZ; Chollangi S; Muller W; Ash JD. 2009. Preconditioning-induced protection of photoreceptors requires activation of the signal-transducing receptor gp130 in photoreceptors. Proc Natl Acad Sci U S A 106(50):21389-94. [PubMed: 19948961] [MGI Ref ID J:155525]
Whitney IE; Raven MA; Ciobanu DC; Poche RA; Ding Q; Elshatory Y; Gan L; Williams RW; Reese BE. 2011. Genetic modulation of horizontal cell number in the mouse retina. Proc Natl Acad Sci U S A 108(23):9697-702. [PubMed: 21576457] [MGI Ref ID J:173233]
Zhang J; Gray J; Wu L; Leone G; Rowan S; Cepko CL; Zhu X; Craft CM; Dyer MA. 2004. Rb regulates proliferation and rod photoreceptor development in the mouse retina. Nat Genet 36(4):351-60. [PubMed: 14991054] [MGI Ref ID J:109491]
Zhang J; Schweers B; Dyer MA. 2004. The first knockout mouse model of retinoblastoma. Cell Cycle 3(7):952-9. [PubMed: 15190215] [MGI Ref ID J:103618]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2450.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Embryos
Price (US dollars $) Frozen Embryo $1600.00 Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryopreserved Embryos
Available to most shipping destinations1
This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.
1 Shipments cannot be made to Australia due to Australian government import restrictions.
2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $3185.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Embryos
Price (US dollars $) Frozen Embryo $2080.00 Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryopreserved Embryos
Available to most shipping destinations1
This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.
1 Shipments cannot be made to Australia due to Australian government import restrictions.
2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
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| Considerations for Choosing Controls | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.