Strain Name: |
STOCK Tg(Chx10-EGFP/cre-ALPP)2Clc/J |
|---|---|
Stock Number: |
005105 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Former Name |
STOCK Tg(Chx10-GFP/cre, ALPP)2Clc/J (Changed: 15-DEC-04
) |
| Genes & Alleles | ALPP; GFP; Vsx2; cre; Tg(Chx10-EGFP/cre-ALPP)2Clc; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator Connie Cepko, Harvard Medical School, HHMI Strain Description
Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The transgene insert contains a fusion product involving Cre recombinase and an Enhanced Green Fluorescent Protein (EGFP) and a fusion product involving an internal ribosome entry site/human placental alkaline phosphatase under the control of the mouse Chx10, C. elegans ceh-10 homeo domain containing homolog promoter. This strain serves as a multifunctional reporter strain. Expression of the Chx10 BAC, as detected by in situ hybridization, mimics the endogenous Chx10 gene expression pattern. Alkaline phosphatase expression is mosaic, but specific to the retina and Muller glial cells. EGFP expression is detected in the outer neuroblastic layer of the retina at embryonic days 14.5, 17.5 and neonates. When crossed to a cre reporter strain, the resulting mice exhibit mosaicism in reporter gene expression. This mutant mouse strain may be useful in studies of retinal progenitor and bipolar cell lineage or fate mapping.Strain Development
A transgenic construct encoding an EGFP/cre fusion protein, an internal ribosome entry site/human placental alkaline phosphatase fusion protein and an FRT-flanked selection cassette (composed of neomycin resistance and kanamycin resistance genes under the control of phosphoglycerate kinase and Tn5 transposon promoter), was inserted into a BAC encoding the mouse Chx10, C. elegans ceh-10 homeo domain containing homolog promoter. This modified BAC transgene was microinjected into B6;SJL fertilized eggs. The founder animals were bred to FVB. The mice were crossed to 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J mice (see Stock No. 003946) to remove the FRT-flanked selection cassette, and then later crossed to C57BL/6 for one generation.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(Chx10-EGFP/cre-ALPP)2Clc | ||
|---|---|---|---|
| Allele Name | transgene insertion 2, Constance L Cepko | ||
| Common Name(s) | Chx10 BAC; Chx10 BAC line 2; Chx10-cre; Tg(Chx10-GFP/cre, ALPP)2Clc/J; | ||
| Strain of Origin | C57BL/6 and SJL | ||
| Site of Expression | expression pattern is mosaic, but specific to the retina and Muller glial cells | ||
| Expressed Gene | ALPP, alkaline phosphatase, placental (Regan isozyme), human | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Vsx2, visual system homeobox 2, rat | ||
| General Note |
According to Rowan and Cepko (2004), the modified BAC DNA "was injected into male pronuclei of SJL/B6 fertilized eggs." Two transgenic lines were generated. Line 1 exhibited highly mosaic expression of the EGFP reporter. Line 2 expressed EGFP more uniformly, but weakly; excision of the FRT-neo cassette increased EGFP expression in subsequent generations, so line 2 was used for further studies. Hemizygous transgenic mice are viable, fertile, normal in size and do not display any gross physical orbehavioral abnormalities. Alkaline phosphatase expression is mosaic, but specific to the retina, and is also detected in Muller glial cells. Green Fluorescent Protein (GFP) expression is detected in the outer neuroblastic layer of the retina at embryonic days 14.5, 17.5 and neonates. When crossed to a cre reporter strain, the resulting mice exhibit mosaicism in reporter gene expression. | ||
| Molecular Note | This reporter gene expresses an enhanced green fluorescent protein-Cre recombinase fusion protein and alkaline phosphatase under control of Chx10 promoter and enhancer elements.The transgene was generated in a 129/Sv-derived bacterial artificial chromosome (BAC) containing the Chx10 gene and extending approximately 55 kb upstream of the translation start site and about 22 kb downstream of the polyadenylation signal. The 8th codon of Chx10 is joined in-frame to an EGFP-cre fusion gene followed by an internal ribosome entry sequence (IRES) coupled to the human placental alkaline phosphatase gene; 32 codons are deleted from within the N-terminal coding sequence of Chx10. The transgene was found by Southern blot analysis to be present in multiple copies, most or all of them intact. [MGI Ref ID J:91498] | ||
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Tg(Chx10-EGFP/cre-ALPP)2Clc
Colony Maintenance
| Diet Information | LabDiet® 5K52/5K67 |
|---|
Related Strains
Fluorescent Protein Strains
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007066 B6.SJL-Tg(Crx-GFP,-ALPP)1Clc/J 006882 STOCK Tg(CAG-Bgeo,-AML1/ETO,-ALPP)1Lbe/J 006613 STOCK Tg(CAG-Bgeo,-Tle1,-ALPP)1Lbe/J 003919 STOCK Tg(CAG-Bgeo/ALPP)1Lbe/J
Additional Web Information
Cre-lox or FLP-FRT Systems
Fluorescent Proteins/lacZ Systems
Research Applications
This mouse can be used to support research in many areas including:
GFP relatedNeurobiology Research
Fluorescent protein expression in neural tissue
Research Tools
Cre-lox System (Cre Recombinase Expression)
Fluorescent Proteins
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Genetics Research (Tissue/Cell Markers)
Genetics Research (Tissue/Cell Markers: Cre-lox System)
Neurobiology Research (cell marker)
Sensorineural Research
cre relatedResearch Tools
Fluorescent Proteins
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
References
Selected Reference(s)
Additional ReferencesRowan S; Cepko CL. 2004. Genetic analysis of the homeodomain transcription factor Chx10 in the retina using a novel multifunctional BAC transgenic mouse reporter. Dev Biol 271(2):388-402. [PubMed: 15223342] [MGI Ref ID J:91498]
Price and Supply Information
| Strain Name: | STOCK Tg(Chx10-EGFP/cre-ALPP)2Clc/J |
| Stock Number: | 005105 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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