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| These transgenic mice have a tamoxifen inducible Cre-mediated recombination system driven by the human keratin 14 (KRT14) promoter, which is active in dividing cells of epidermis and some other stratified squamous epithelia. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring can be used to generate tamoxifen-induced, Cre-mediated targeted keratinocyte-specific deletions. This strain may be useful for studying somatic mutations of the skin, including squamous and basal cell carcinomas. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Former Names STOCK Tg(KRT14-cre/Esr1)20Efu/J (Changed: 01-APR-10 ) STOCK Tg(KRT14-cre/Esr1)20Efu (Changed: 04-MAY-05 ) Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Homozygote (Female x Male) 01-MAR-06 Species laboratory mouse Generation F?+14 (24-MAR-11)
Generation DefinitionsDonating Investigator Elaine Fuchs, The Rockefeller University Description
These transgenic mice have a tamoxifen inducible Cre-mediated recombination system driven by the human keratin 14 (KRT14) promoter. The transgene insert contains a fusion product involving Cre recombinase and a mutant form of the mouse estrogen receptor ligand binding domain. The mutant mouse estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen (tamoxifen). Restricted to the cytoplasm, the cre/Esr1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted keratinocyte-specific deletions. Oral tamoxifen administration induces Cre recombination in toe, back skin and tongue. Topically administered tamoxifen induces Cre-mediated recombination in a specific localized area of the skin, occuring in 50 to 60% of the cells in the epidermis. Hemizygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.View cre expression characterization.
Development
A transgenic construct containing sequence encoding cre/Esr1 under the control of the human keratin 14 (KRT14) promoter, was introduced into CD-1 donor eggs. Founder line 20 was subsequently established.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| See control note: | This strain is on a background of the random bred CD1 strain. There is no appropriate control for this strain. | |
| Considerations for Choosing Controls | ||
Related Strains
Facebase: tools
006084 B6.129P2(Cg)-Foxg1tm1(cre)Skm/J 003771 B6.Cg-Tg(Nes-cre)1Kln/J 005584 B6.Cg-Tg(Prrx1-cre)1Cjt/J 009388 B6;129S1-Osr2tm2(cre)Jian/J 006774 FVB-Tg(Col2a1-cre/ERT)KA3Smac/J 012719 STOCK Tgfb3tm1(cre)Vk/J 004782 STOCK Tg(KRT14-cre)1Amc/J 003829 STOCK Tg(Wnt1-cre)11Rth Tg(Wnt1-GAL4)11Rth/J View Facebase: tools (8 strains)
006403 129S.B6-Tg(KRT14-Esr1/HRAS)1Pkha/J 006661 129S.B6-Tg(KRT14-RAF1/ESR1)1Pkha/J 004853 B6.C3-Tg(KRT14-Birc5)19Gros/J 009687 B6.Cg-Tg(KRT14-Kitl*)4XTG2Bjl/J 007622 B6;SJL-Tg(KRT14-Hr)551Cct/J 007678 B6;SJL-Tg(KRT14-rtTA)208Jek/J 002980 C57BL/6-Tg(Cd152Ig)1Jbs/J 006822 FVB-Tg(KRT14-MAP2K1/Esr1)12Pkha/J 005705 FVB-Tg(KRT14-Vegfa)3Dtm/J 008099 FVB-Tg(KRT14-rtTA)F42Efu/J 017005 FVB.Cg-Tg(KRT14-Fyn*)aJsey/J 004782 STOCK Tg(KRT14-cre)1Amc/J
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedDermatology Research
Other
Developmental Biology Research
Craniofacial and Palate Defects
Orofacial clefting-specific cre expression
Research Tools
Cre-lox System
Cre Recombinase Expression
Cre Recombinase Expression: Inducible
Dermatology Research
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(KRT14-cre/ERT)20Efu | ||
|---|---|---|---|
| Allele Name | transgene insertion 20, Elaine Fuchs | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | K14-CreERtam; K14-CreERT; K14CreERTam; Ker14-CreERT; Tg(KRT14-cre/Esr1)2Efu; | ||
| Mutation Made By | Valeri Vasioukhin, Fred Hutchinson Cancer Research Center | ||
| Site of Expression | when crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted keratinocyte-specific deletions | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | KRT14, keratin 14, human | ||
| Gene Symbol and Name | Tg(KRT14-cre/ERT)20Efu, transgene insertion 20, Elaine Fuchs | ||
| Chromosome | UN | ||
| Gene Common Name(s) | K14-CreERtam; K14-CreERT; Tg(KRT14-cre/Esr1)20Efu; Tg(KRT14-cre/Esr1)2Efu; transgene insertion 2, Elaine Fuchs; | ||
| Driver Note | KRT14 | ||
| Inducible Note | Induced by tamoxifen. | ||
| Molecular Note | Expression of a tamoxifen-inducible form of a Cre recombinase and estrogen receptor fusion protein in the basal layer of the epidermis, the outer root sheath of the hair follicle and the oral epithelium, driven by the human keratin 14 promoter (KRT14). Promoter is active by embryonic day 14.5 (E14.5). [MGI Ref ID J:56822] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Quantitative PCR, QPCR
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Vasioukhin V; Degenstein L; Wise B; Fuchs E. 1999. The magical touch: genome targeting in epidermal stem cells induced by tamoxifen application to mouse skin. Proc Natl Acad Sci U S A 96(15):8551-6. [PubMed: 10411913] [MGI Ref ID J:56822]
Additional References
Tg(KRT14-cre/ERT)20Efu relatedAmbler CA; Watt FM. 2010. Adult epidermal Notch activity induces dermal accumulation of T cells and neural crest derivatives through upregulation of jagged 1. Development 137(21):3569-79. [PubMed: 20940224] [MGI Ref ID J:165557]
Benitah SA; Frye M; Glogauer M; Watt FM. 2005. Stem cell depletion through epidermal deletion of Rac1. Science 309(5736):933-5. [PubMed: 16081735] [MGI Ref ID J:99995]
Benitah SA; Watt FM. 2007. Epidermal deletion of Rac1 causes stem cell depletion, irrespective of whether deletion occurs during embryogenesis or adulthood. J Invest Dermatol 127(6):1555-7. [PubMed: 17301832] [MGI Ref ID J:121550]
Bian Y; Hall B; Sun ZJ; Molinolo A; Chen W; Gutkind JS; Waes CV; Kulkarni AB. 2012. Loss of TGF-beta signaling and PTEN promotes head and neck squamous cell carcinoma through cellular senescence evasion and cancer-related inflammation. Oncogene 31(28):3322-32. [PubMed: 22037217] [MGI Ref ID J:194578]
Bian Y; Terse A; Du J; Hall B; Molinolo A; Zhang P; Chen W; Flanders KC; Gutkind JS; Wakefield LM; Kulkarni AB. 2009. Progressive tumor formation in mice with conditional deletion of TGF-beta signaling in head and neck epithelia is associated with activation of the PI3K/Akt pathway. Cancer Res 69(14):5918-26. [PubMed: 19584284] [MGI Ref ID J:150640]
Boutin AT; Weidemann A; Fu Z; Mesropian L; Gradin K; Jamora C; Wiesener M; Eckardt KU; Koch CJ; Ellies LG; Haddad G; Haase VH; Simon MC; Poellinger L; Powell FL; Johnson RS. 2008. Epidermal sensing of oxygen is essential for systemic hypoxic response. Cell 133(2):223-34. [PubMed: 18423195] [MGI Ref ID J:145307]
Choi N; Zhang B; Zhang L; Ittmann M; Xin L. 2012. Adult Murine Prostate Basal and Luminal Cells Are Self-Sustained Lineages that Can Both Serve as Targets for Prostate Cancer Initiation. Cancer Cell 21(2):253-65. [PubMed: 22340597] [MGI Ref ID J:181459]
Collins CA; Watt FM. 2008. Dynamic regulation of retinoic acid-binding proteins in developing, adult and neoplastic skin reveals roles for beta-catenin and Notch signalling. Dev Biol 324(1):55-67. [PubMed: 18805411] [MGI Ref ID J:142873]
Croyle MJ; Lehman JM; O'Connor AK; Wong SY; Malarkey EB; Iribarne D; Dowdle WE; Schoeb TR; Verney ZM; Athar M; Michaud EJ; Reiter JF; Yoder BK. 2011. Role of epidermal primary cilia in the homeostasis of skin and hair follicles. Development 138(9):1675-85. [PubMed: 21429982] [MGI Ref ID J:171221]
Demehri S; Turkoz A; Kopan R. 2009. Epidermal Notch1 loss promotes skin tumorigenesis by impacting the stromal microenvironment. Cancer Cell 16(1):55-66. [PubMed: 19573812] [MGI Ref ID J:150340]
Driskell I; Oda H; Blanco S; Nascimento E; Humphreys P; Frye M. 2012. The histone methyltransferase Setd8 acts in concert with c-Myc and is required to maintain skin. EMBO J 31(3):616-29. [PubMed: 22117221] [MGI Ref ID J:181779]
Estrach S; Ambler CA; Lo Celso CL; Hozumi K; Watt FM. 2006. Jagged 1 is a {beta}-catenin target gene required for ectopic hair follicle formation in adult epidermis. Development 133(22):4427-38. [PubMed: 17035290] [MGI Ref ID J:115280]
Fujioka M; Tokano H; Fujioka KS; Okano H; Edge AS. 2011. Generating mouse models of degenerative diseases using Cre/lox-mediated in vivo mosaic cell ablation. J Clin Invest 121(6):2462-9. [PubMed: 21576819] [MGI Ref ID J:173515]
Gu D; Fan Q; Zhang X; Xie J. 2012. A role for transcription factor STAT3 signaling in oncogene smoothened-driven carcinogenesis. J Biol Chem 287(45):38356-66. [PubMed: 22992748] [MGI Ref ID J:192468]
House JS; Zhu S; Ranjan R; Linder K; Smart RC. 2010. C/EBPalpha and C/EBPbeta are required for Sebocyte differentiation and stratified squamous differentiation in adult mouse skin. PLoS One 5(3):e9837. [PubMed: 20352127] [MGI Ref ID J:158721]
Hunziker L; Aznar Benitah S; Braun KM; Jensen K; McNulty K; Butler C; Potton E; Nye E; Boyd R; Laurent G; Glogauer M; Wright NA; Watt FM; Janes SM. 2011. Rac1 deletion causes thymic atrophy. PLoS One 6(4):e19292. [PubMed: 21559396] [MGI Ref ID J:172344]
Jamieson T; Clarke M; Steele CW; Samuel MS; Neumann J; Jung A; Huels D; Olson MF; Das S; Nibbs RJ; Sansom OJ. 2012. Inhibition of CXCR2 profoundly suppresses inflammation-driven and spontaneous tumorigenesis. J Clin Invest 122(9):3127-44. [PubMed: 22922255] [MGI Ref ID J:190744]
Kitamura H; Cambier S; Somanath S; Barker T; Minagawa S; Markovics J; Goodsell A; Publicover J; Reichardt L; Jablons D; Wolters P; Hill A; Marks JD; Lou J; Pittet JF; Gauldie J; Baron JL; Nishimura SL. 2011. Mouse and human lung fibroblasts regulate dendritic cell trafficking, airway inflammation, and fibrosis through integrin alphavbeta8-mediated activation of TGF-beta. J Clin Invest 121(7):2863-75. [PubMed: 21646718] [MGI Ref ID J:175658]
Kobielak K; Stokes N; de la Cruz J; Polak L; Fuchs E. 2007. Loss of a quiescent niche but not follicle stem cells in the absence of bone morphogenetic protein signaling. Proc Natl Acad Sci U S A 104(24):10063-8. [PubMed: 17553962] [MGI Ref ID J:142940]
Lapouge G; Youssef KK; Vokaer B; Achouri Y; Michaux C; Sotiropoulou PA; Blanpain C. 2011. Identifying the cellular origin of squamous skin tumors. Proc Natl Acad Sci U S A 108(18):7431-6. [PubMed: 21502497] [MGI Ref ID J:172048]
Lehoczky JA; Robert B; Tabin CJ. 2011. Mouse digit tip regeneration is mediated by fate-restricted progenitor cells. Proc Natl Acad Sci U S A 108(51):20609-14. [PubMed: 22143790] [MGI Ref ID J:180520]
Liu L; Lee S; Zhang J; Peters SB; Hannah J; Zhang Y; Yin Y; Koff A; Ma L; Zhou P. 2009. CUL4A abrogation augments DNA damage response and protection against skin carcinogenesis. Mol Cell 34(4):451-60. [PubMed: 19481525] [MGI Ref ID J:150427]
Lopez-Rovira T; Silva-Vargas V; Watt FM. 2005. Different Consequences of beta1 Integrin Deletion in Neonatal and Adult Mouse Epidermis Reveal a Context-Dependent Role of Integrins in Regulating Proliferation, Differentiation, and Intercellular Communication. J Invest Dermatol 125(6):1215-27. [PubMed: 16354192] [MGI Ref ID J:103202]
Lu CP; Polak L; Rocha AS; Pasolli HA; Chen SC; Sharma N; Blanpain C; Fuchs E. 2012. Identification of stem cell populations in sweat glands and ducts reveals roles in homeostasis and wound repair. Cell 150(1):136-50. [PubMed: 22770217] [MGI Ref ID J:186240]
Nascimento EM; Cox CL; Macarthur S; Hussain S; Trotter M; Blanco S; Suraj M; Nichols J; Kubler B; Benitah SA; Hendrich B; Odom DT; Frye M. 2011. The opposing transcriptional functions of Sin3a and c-Myc are required to maintain tissue homeostasis. Nat Cell Biol 13(12):1395-405. [PubMed: 22101514] [MGI Ref ID J:178941]
Nishio M; Hamada K; Kawahara K; Sasaki M; Noguchi F; Chiba S; Mizuno K; Suzuki SO; Dong Y; Tokuda M; Morikawa T; Hikasa H; Eggenschwiler J; Yabuta N; Nojima H; Nakagawa K; Hata Y; Nishina H; Mimori K; Mori M; Sasaki T; Mak TW; Nakano T; Itami S; Suzuki A. 2012. Cancer susceptibility and embryonic lethality in Mob1a/1b double-mutant mice. J Clin Invest 122(12):4505-18. [PubMed: 23143302] [MGI Ref ID J:193981]
Omori E; Inagaki M; Mishina Y; Matsumoto K; Ninomiya-Tsuji J. 2012. Epithelial transforming growth factor beta-activated kinase 1 (TAK1) is activated through two independent mechanisms and regulates reactive oxygen species. Proc Natl Acad Sci U S A 109(9):3365-70. [PubMed: 22331902] [MGI Ref ID J:182165]
Omori E; Matsumoto K; Ninomiya-Tsuji J. 2011. Non-canonical beta-catenin degradation mediates reactive oxygen species-induced epidermal cell death. Oncogene 30(30):3336-44. [PubMed: 21383695] [MGI Ref ID J:174637]
Omori E; Matsumoto K; Zhu S; Smart RC; Ninomiya-Tsuji J. 2010. Ablation of TAK1 upregulates reactive oxygen species and selectively kills tumor cells. Cancer Res 70(21):8417-25. [PubMed: 20959492] [MGI Ref ID J:166056]
Omori E; Morioka S; Matsumoto K; Ninomiya-Tsuji J. 2008. TAK1 regulates reactive oxygen species and cell death in keratinocytes, which is essential for skin integrity. J Biol Chem 283(38):26161-8. [PubMed: 18606807] [MGI Ref ID J:142025]
Paul E; Cronan R; Weston PJ; Boekelheide K; Sedivy JM; Lee SY; Wiest DL; Resnick MB; Klysik JE. 2009. Disruption of Supv3L1 damages the skin and causes sarcopenia, loss of fat, and death. Mamm Genome 20(2):92-108. [PubMed: 19145458] [MGI Ref ID J:144991]
Pazolli E; Luo X; Brehm S; Carbery K; Chung JJ; Prior JL; Doherty J; Demehri S; Salavaggione L; Piwnica-Worms D; Stewart SA. 2009. Senescent stromal-derived osteopontin promotes preneoplastic cell growth. Cancer Res 69(3):1230-9. [PubMed: 19155301] [MGI Ref ID J:144976]
Poulson ND; Lechler T. 2010. Robust control of mitotic spindle orientation in the developing epidermis. J Cell Biol 191(5):915-22. [PubMed: 21098114] [MGI Ref ID J:166997]
Rabbani P; Takeo M; Chou W; Myung P; Bosenberg M; Chin L; Taketo MM; Ito M. 2011. Coordinated activation of wnt in epithelial and melanocyte stem cells initiates pigmented hair regeneration. Cell 145(6):941-55. [PubMed: 21663796] [MGI Ref ID J:173080]
Raimondi AR; Molinolo A; Gutkind JS. 2009. Rapamycin prevents early onset of tumorigenesis in an oral-specific K-ras and p53 two-hit carcinogenesis model. Cancer Res 69(10):4159-66. [PubMed: 19435901] [MGI Ref ID J:148476]
Rinkevich Y; Lindau P; Ueno H; Longaker MT; Weissman IL. 2011. Germ-layer and lineage-restricted stem/progenitors regenerate the mouse digit tip. Nature 476(7361):409-13. [PubMed: 21866153] [MGI Ref ID J:188630]
Shin MK; Pitot HC; Lambert PF. 2012. Pocket proteins suppress head and neck cancer. Cancer Res 72(5):1280-9. [PubMed: 22237625] [MGI Ref ID J:181495]
Shin MK; Sage J; Lambert PF. 2012. Inactivating all three rb family pocket proteins is insufficient to initiate cervical cancer. Cancer Res 72(20):5418-27. [PubMed: 22942253] [MGI Ref ID J:192917]
Silva-Vargas V; Lo Celso C; Giangreco A; Ofstad T; Prowse DM; Braun KM; Watt FM. 2005. Beta-catenin and Hedgehog signal strength can specify number and location of hair follicles in adult epidermis without recruitment of bulge stem cells. Dev Cell 9(1):121-31. [PubMed: 15992546] [MGI Ref ID J:129791]
Spassov DS; Wong CH; Wong SY; Reiter JF; Moasser MM. 2013. Trask loss enhances tumorigenic growth by liberating integrin signaling and growth factor receptor cross-talk in unanchored cells. Cancer Res 73(3):1168-79. [PubMed: 23243018] [MGI Ref ID J:194368]
Sun ZJ; Zhang L; Hall B; Bian Y; Gutkind JS; Kulkarni AB. 2012. Chemopreventive and chemotherapeutic actions of mTOR inhibitor in genetically defined head and neck squamous cell carcinoma mouse model. Clin Cancer Res 18(19):5304-13. [PubMed: 22859719] [MGI Ref ID J:194652]
Van Keymeulen A; Mascre G; Youseff KK; Harel I; Michaux C; De Geest N; Szpalski C; Achouri Y; Bloch W; Hassan BA; Blanpain C. 2009. Epidermal progenitors give rise to Merkel cells during embryonic development and adult homeostasis. J Cell Biol 187(1):91-100. [PubMed: 19786578] [MGI Ref ID J:163216]
Wang X; Ouyang H; Yamamoto Y; Kumar PA; Wei TS; Dagher R; Vincent M; Lu X; Bellizzi AM; Ho KY; Crum CP; Xian W; McKeon F. 2011. Residual Embryonic Cells as Precursors of a Barrett's-like Metaplasia. Cell 145(7):1023-35. [PubMed: 21703447] [MGI Ref ID J:173368]
Wang XP; O'Connell DJ; Lund JJ; Saadi I; Kuraguchi M; Turbe-Doan A; Cavallesco R; Kim H; Park PJ; Harada H; Kucherlapati R; Maas RL. 2009. Apc inhibition of Wnt signaling regulates supernumerary tooth formation during embryogenesis and throughout adulthood. Development 136(11):1939-49. [PubMed: 19429790] [MGI Ref ID J:149542]
Wong SY; Reiter JF. 2011. From the Cover: Wounding mobilizes hair follicle stem cells to form tumors. Proc Natl Acad Sci U S A 108(10):4093-8. [PubMed: 21321207] [MGI Ref ID J:170482]
Wong SY; Seol AD; So PL; Ermilov AN; Bichakjian CK; Epstein EH Jr; Dlugosz AA; Reiter JF. 2009. Primary cilia can both mediate and suppress Hedgehog pathway-dependent tumorigenesis. Nat Med 15(9):1055-61. [PubMed: 19701205] [MGI Ref ID J:154128]
Yang C; Hayashida T; Forster N; Li C; Shen D; Maheswaran S; Chen L; Anderson KS; Ellisen LW; Sgroi D; Schmidt EV. 2011. The integrin alpha(v)beta(3-5) ligand MFG-E8 is a p63/p73 target gene in triple-negative breast cancers but exhibits suppressive functions in ER(+) and erbB2(+) breast cancers. Cancer Res 71(3):937-45. [PubMed: 21127199] [MGI Ref ID J:169326]
Youssef KK; Lapouge G; Bouvree K; Rorive S; Brohee S; Appelstein O; Larsimont JC; Sukumaran V; Van de Sande B; Pucci D; Dekoninck S; Berthe JV; Aerts S; Salmon I; del Marmol V; Blanpain C. 2012. Adult interfollicular tumour-initiating cells are reprogrammed into an embryonic hair follicle progenitor-like fate during basal cell carcinoma initiation. Nat Cell Biol 14(12):1282-94. [PubMed: 23178882] [MGI Ref ID J:195242]
Youssef KK; Van Keymeulen A; Lapouge G; Beck B; Michaux C; Achouri Y; Sotiropoulou PA; Blanpain C. 2010. Identification of the cell lineage at the origin of basal cell carcinoma. Nat Cell Biol 12(3):299-305. [PubMed: 20154679] [MGI Ref ID J:195334]
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Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous mice may be bred. Mating System Homozygote x Homozygote (Female x Male) 01-MAR-06 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Homozygous for Tg(KRT14-cre/Esr1)20Efu
Price per Pair (US dollars $) Pair Genotype $464.00 Homozygous for Tg(KRT14-cre/Esr1)20Efu x Homozygous for Tg(KRT14-cre/Esr1)20Efu Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Homozygous for Tg(KRT14-cre/Esr1)20Efu
Price per Pair (US dollars $) Pair Genotype $603.20 Homozygous for Tg(KRT14-cre/Esr1)20Efu x Homozygous for Tg(KRT14-cre/Esr1)20Efu Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
|
|
|
Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| See control note: | This strain is on a background of the random bred CD1 strain. There is no appropriate control for this strain. | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.