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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names STOCK Tg(KRT14-cre/Esr1)20Efu (Changed: 04-MAY-05 ) Type Mutant Stock; Transgenic; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Generation F?+9 (31-DEC-07) Donating Investigator Elaine Fuchs, The Rockefeller University Description
These transgenic mice have a tamoxifen inducible Cre-mediated recombination system driven by the human keratin 14 (KRT14) promoter. The transgene insert contains a fusion product involving Cre recombinase and a mutant form of the mouse estrogen receptor ligand binding domain. The mutant mouse estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen (tamoxifen). Restricted to the cytoplasm, the cre/Esr1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted keratinocyte-specific deletions. Oral tamoxifen administration induces Cre recombination in toe, back skin and tongue. Topically administered tamoxifen induces Cre-mediated recombination in a specific localized area of the skin, occuring in 50 to 60% of the cells in the epidermis. Hemizygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.Development
A transgenic construct containing sequence encoding cre/Esr1 under the control of the human keratin 14 (KRT14) promoter, was introduced into CD-1 donor eggs. Founder line 20 was subsequently established.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| This strain is on a background of the random bred CD1 strain. There is no appropriate control for this strain. | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Esr1
006403 129S.B6-Tg(KRT14-Esr1/HRAS)1Pkha/J 004744 B6.129P2-Esr1tm1Ksk/J 004682 B6.Cg-Tg(CAG-cre/Esr1)5Amc/J 005657 B6.Cg-Tg(Myh6-cre/Esr1)1Jmk/J 005650 B6129-Tg(Myh6-cre/Esr1)1Jmk/J 006774 FVB-Tg(Col2a1-cre/ESR1)KA3Smac/J 006822 FVB-Tg(KRT14-MAP2K1/Esr1)12Pkha/J 008464 STOCK Foxa2tm2.1(cre/Esr1)Moon/J 004453 STOCK Tg(CAG-cre/Esr1)5Amc/J 008122 STOCK Tg(Ins2-cre/Esr1)1Dam/J 008119 STOCK Tg(Neurog3-cre/Esr1)1Dam/J View Strains carrying other alleles of Esr1 (11 strains)
006403 129S.B6-Tg(KRT14-Esr1/HRAS)1Pkha/J 006661 129S.B6-Tg(KRT14-RAF1/ESR1)1Pkha/J 004853 B6.C3-Tg(KRT14-Birc5)19Gros/J 007622 B6;SJL-Tg(KRT14-Hr)551Cct/J 007678 B6;SJL-Tg(KRT14-rtTA)208Jek/J 002980 C57BL/6-Tg(Cd152Ig)1Jbs/J 006822 FVB-Tg(KRT14-MAP2K1/Esr1)12Pkha/J 005705 FVB-Tg(KRT14-Vegfa)3Dtm/J 008099 FVB-Tg(KRT14-rtTA)F42Efu/J 004782 STOCK Tg(KRT14-cre)1Amc/J
Additional Web Information
Cre-lox Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedDermatology Research
Other
Research Tools
Cre-lox System (Cre-Recombinase Expression: Inducible)
Dermatology Research
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(KRT14-cre/Esr1)20Efu | ||
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| Allele Name | transgene insertion 20, Elaine Fuchs | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | K14-CreERtam; Tg(KRT14-cre/Esr1)2Efu; | ||
| Mutation Made By | Valera Vasioukhin, Fred Hutchinson Cancer Research Center | ||
| Site of Expression | when crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted keratinocyte-specific deletions | ||
| Expressed Gene | Esr1, estrogen receptor 1 (alpha), mouse, laboratory | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | KRT14, keratin 14, human | ||
| Molecular Note | Expression of a tamoxifen-inducible form of a Cre recombinase and estrogen receptor fusion protein in the basal layer of the epidermis, the outer root sheath of the hair follicle and the oral epithelium, driven by the human keratin 14 promoter (KRT14). Promoter is active by embryonic day 14.5 (E14.5). [MGI Ref ID J:56822] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Quantitative PCR, QPCR, vers. 1
Generic Cre, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Vasioukhin V; Degenstein L; Wise B; Fuchs E. 1999. The magical touch: genome targeting in epidermal stem cells induced by tamoxifen application to mouse skin. Proc Natl Acad Sci U S A 96(15):8551-6. [PubMed: 10411913] [MGI Ref ID J:56822]
Additional References
Tg(KRT14-cre/Esr1)20Efu relatedBenitah SA; Frye M; Glogauer M; Watt FM. 2005. Stem cell depletion through epidermal deletion of Rac1. Science 309(5736):933-5. [PubMed: 16081735] [MGI Ref ID J:99995]
Benitah SA; Watt FM. 2007. Epidermal deletion of Rac1 causes stem cell depletion, irrespective of whether deletion occurs during embryogenesis or adulthood. J Invest Dermatol 127(6):1555-7. [PubMed: 17301832] [MGI Ref ID J:121550]
Estrach S; Ambler CA; Lo Celso CL; Hozumi K; Watt FM. 2006. Jagged 1 is a {beta}-catenin target gene required for ectopic hair follicle formation in adult epidermis. Development 133(22):4427-38. [PubMed: 17035290] [MGI Ref ID J:115280]
Lopez-Rovira T; Silva-Vargas V; Watt FM. 2005. Different Consequences of beta1 Integrin Deletion in Neonatal and Adult Mouse Epidermis Reveal a Context-Dependent Role of Integrins in Regulating Proliferation, Differentiation, and Intercellular Communication. J Invest Dermatol 125(6):1215-27. [PubMed: 16354192] [MGI Ref ID J:103202]
Silva-Vargas V; Lo Celso C; Giangreco A; Ofstad T; Prowse DM; Braun KM; Watt FM. 2005. Beta-catenin and Hedgehog signal strength can specify number and location of hair follicles in adult epidermis without recruitment of bulge stem cells. Dev Cell 9(1):121-31. [PubMed: 15992546] [MGI Ref ID J:129791]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous mice may be bred. Mating System Homozygote x Homozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $155.60 Female or Male Homozygous for Tg(KRT14-cre/Esr1)20Efu *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $311.20 Homozygous for Tg(KRT14-cre/Esr1)20Efu x Homozygous for Tg(KRT14-cre/Esr1)20Efu
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $202.30 Female or Male Homozygous for Tg(KRT14-cre/Esr1)20Efu *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $404.60 Homozygous for Tg(KRT14-cre/Esr1)20Efu x Homozygous for Tg(KRT14-cre/Esr1)20Efu
Additional Supply Details
| Supply Notes |
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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Control Information
| Control | ||
|---|---|---|
| None Available | ||
| This strain is on a background of the random bred CD1 strain. There is no appropriate control for this strain. | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Notice Regarding cre/Esr1 (Tamoxifen Inducible Cre) Strains.
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice & Services Conditions of Use
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