Strain Name:

B6.129P2-Ltb/Tnf/Ltatm1Dvk/J

Stock Number:

005108

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
GenerationN13p (10-APR-05)
Generation Definitions
 
Donating Investigator Sergei Nedospasov,   NCI-FCRDC

Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA) from any of the three targeted genes is detected by RT-PCR analysis of concanavalin-A activated splenocytes. Leukocyte numbers in the spleen, blood and peritoneal cavity are increased 2 to 3 fold. Homozygous mice have abnormal lymphoid development and do not develop Peyer's patches or lymph nodes. Spleen microarchitecture is abnormal. No germinal center forms in the spleen following antigenic challenge. Mutant mice display defective antibody responses. This strain displays a phenotype more severe than the phenotypes exhibited by any of the single targeted mutation strains of the genes and may be useful in studies of peripheral lymphoid organ development, and antibody response to T cell dependent antigens.

Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes, and four loxP sites, flanking each of the three genes, was used to disrupt 8.4 kb of sequence encoding all of the Tnf gene and the last exons of the Lta and Ltb genes. The construct was electroporated into 129P2/OlaHsd derived E14.1 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a cre expression plasmid for the purpose of removing the selectable marker cassette. ES cells that had successfully undergone Cre-mediated recombination and no longer retained the cassette were injected into C57BL/6 blastocysts. The donating investigator reported that the resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to for 12 generations (see SNP note below).

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 3 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Lta
002258   B6.129S2-Ltatm1Dch/J
002257   B6;129S2-Ltatm1Dch/J
005579   C57BL/6J-Ltahlb382/J
View Strains carrying other alleles of Lta     (3 strains)

Strains carrying other alleles of Ltb
003530   B6;129-Ltbtm1Flv/J
005112   STOCK Ltb/Tnftm1.1Dvk/J
View Strains carrying other alleles of Ltb     (2 strains)

Strains carrying other alleles of Tnf
005540   B6.129S-Tnftm1Gkl/J
003008   B6;129S-Tnftm1Gkl/J
007082   CByJ.129S(B6)-Tnftm1Gkl/J
005112   STOCK Ltb/Tnftm1.1Dvk/J
View Strains carrying other alleles of Tnf     (4 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Ltb/Tnf/Ltatm1Dvk/Ltb/Tnf/Ltatm1Dvk

        involves: C57BL/6
  • hematopoietic system phenotype
  • decreased spleen white pulp amount
    • percentage of splenic white pulp is lower than in wild-type, Ltb, the two Lta mutant lines, Ltb or Ltbr knockouts   (MGI Ref ID J:109621)
  • immune system phenotype
  • decreased spleen white pulp amount
    • percentage of splenic white pulp is lower than in wild-type, Ltb, the two Lta mutant lines, Ltb or Ltbr knockouts   (MGI Ref ID J:109621)

Ltb/Tnf/Ltatm1Dvk/Ltb/Tnf/Ltatm1Dvk

        B6.Cg-Ltb/Tnf/Ltatm1Dvk
  • immune system phenotype
  • abnormal class switch recombination
    • specific IgG antibodies are almost undetectable in SRBC immunized mutants, indicating defective class switching   (MGI Ref ID J:80616)
  • abnormal spleen morphology
    • spleen architecture is severely disturbed   (MGI Ref ID J:80616)
    • distribution of lymphocytes and their functional compartmentalization are severely disorganized in the spleen, with IgD+ B cells scattered along red and white pulp and T cells mostly condensed around central arterioles   (MGI Ref ID J:80616)
    • abnormal spleen B cell follicle morphology
      • absence of polarized B-cell follicles   (MGI Ref ID J:80616)
      • absent spleen germinal center
        • do not form germinal centers   (MGI Ref ID J:80616)
    • absent spleen marginal zone   (MGI Ref ID J:80616)
    • decreased spleen white pulp amount
      • gradual reduction of white pulp size   (MGI Ref ID J:80616)
  • absent Peyer's patches   (MGI Ref ID J:80616)
  • absent axillary lymph nodes   (MGI Ref ID J:80616)
  • absent brachial lymph nodes   (MGI Ref ID J:80616)
  • absent inguinal lymph nodes   (MGI Ref ID J:80616)
  • absent lymph nodes   (MGI Ref ID J:80616)
  • absent mesenteric lymph nodes   (MGI Ref ID J:80616)
  • absent popliteal lymph nodes   (MGI Ref ID J:80616)
  • decreased IgG level
    • specific IgG antibodies are almost undetectable in SRBC immunized mutants   (MGI Ref ID J:80616)
  • increased leukocyte cell number
    • 2-3 fold increase in the number of leukocytes in the spleen, blood, and peritoneal cavity   (MGI Ref ID J:80616)
  • increased susceptibility to bacterial infection
    • increased susceptibility to Listeria monocytogenes infections, with mutants dying by day 8 while wild-type mice survive   (MGI Ref ID J:80616)
    • increased susceptibility to Salmonella enterica infection   (MGI Ref ID J:80616)
  • hematopoietic system phenotype
  • abnormal class switch recombination
    • specific IgG antibodies are almost undetectable in SRBC immunized mutants, indicating defective class switching   (MGI Ref ID J:80616)
  • abnormal spleen morphology
    • spleen architecture is severely disturbed   (MGI Ref ID J:80616)
    • distribution of lymphocytes and their functional compartmentalization are severely disorganized in the spleen, with IgD+ B cells scattered along red and white pulp and T cells mostly condensed around central arterioles   (MGI Ref ID J:80616)
    • abnormal spleen B cell follicle morphology
      • absence of polarized B-cell follicles   (MGI Ref ID J:80616)
      • absent spleen germinal center
        • do not form germinal centers   (MGI Ref ID J:80616)
    • absent spleen marginal zone   (MGI Ref ID J:80616)
    • decreased spleen white pulp amount
      • gradual reduction of white pulp size   (MGI Ref ID J:80616)
  • decreased IgG level
    • specific IgG antibodies are almost undetectable in SRBC immunized mutants   (MGI Ref ID J:80616)
  • increased leukocyte cell number
    • 2-3 fold increase in the number of leukocytes in the spleen, blood, and peritoneal cavity   (MGI Ref ID J:80616)

Ltb/Tnf/Ltatm1Dvk/Ltb/Tnf/Ltatm1Dvk

        B6.Cg-Ltb/Tnf/Ltatm1Dvk
  • immune system phenotype
  • abnormal class switch recombination
    • specific IgG antibodies are almost undetectable in SRBC immunized mutants, indicating defective class switching   (MGI Ref ID J:80616)
  • abnormal spleen morphology
    • spleen architecture is severely disturbed   (MGI Ref ID J:80616)
    • distribution of lymphocytes and their functional compartmentalization are severely disorganized in the spleen, with IgD+ B cells scattered along red and white pulp and T cells mostly condensed around central arterioles   (MGI Ref ID J:80616)
    • abnormal spleen B cell follicle morphology
      • absence of polarized B-cell follicles   (MGI Ref ID J:80616)
      • absent spleen germinal center
        • do not form germinal centers   (MGI Ref ID J:80616)
    • absent spleen marginal zone   (MGI Ref ID J:80616)
    • decreased spleen white pulp amount
      • gradual reduction of white pulp size   (MGI Ref ID J:80616)
  • absent Peyer's patches   (MGI Ref ID J:80616)
  • absent axillary lymph nodes   (MGI Ref ID J:80616)
  • absent brachial lymph nodes   (MGI Ref ID J:80616)
  • absent inguinal lymph nodes   (MGI Ref ID J:80616)
  • absent lymph nodes   (MGI Ref ID J:80616)
  • absent mesenteric lymph nodes   (MGI Ref ID J:80616)
  • absent popliteal lymph nodes   (MGI Ref ID J:80616)
  • decreased IgG level
    • specific IgG antibodies are almost undetectable in SRBC immunized mutants   (MGI Ref ID J:80616)
  • increased leukocyte cell number
    • 2-3 fold increase in the number of leukocytes in the spleen, blood, and peritoneal cavity   (MGI Ref ID J:80616)
  • increased susceptibility to bacterial infection
    • increased susceptibility to Listeria monocytogenes infections, with mutants dying by day 8 while wild-type mice survive   (MGI Ref ID J:80616)
    • increased susceptibility to Salmonella enterica infection   (MGI Ref ID J:80616)
  • hematopoietic system phenotype
  • abnormal class switch recombination
    • specific IgG antibodies are almost undetectable in SRBC immunized mutants, indicating defective class switching   (MGI Ref ID J:80616)
  • abnormal spleen morphology
    • spleen architecture is severely disturbed   (MGI Ref ID J:80616)
    • distribution of lymphocytes and their functional compartmentalization are severely disorganized in the spleen, with IgD+ B cells scattered along red and white pulp and T cells mostly condensed around central arterioles   (MGI Ref ID J:80616)
    • abnormal spleen B cell follicle morphology
      • absence of polarized B-cell follicles   (MGI Ref ID J:80616)
      • absent spleen germinal center
        • do not form germinal centers   (MGI Ref ID J:80616)
    • absent spleen marginal zone   (MGI Ref ID J:80616)
    • decreased spleen white pulp amount
      • gradual reduction of white pulp size   (MGI Ref ID J:80616)
  • decreased IgG level
    • specific IgG antibodies are almost undetectable in SRBC immunized mutants   (MGI Ref ID J:80616)
  • increased leukocyte cell number
    • 2-3 fold increase in the number of leukocytes in the spleen, blood, and peritoneal cavity   (MGI Ref ID J:80616)

Ltb/Tnf/Ltatm1Dvk/Ltb/Tnf/Ltatm1Dvk

        B6.Cg-Ltb/Tnf/Ltatm1Dvk
  • immune system phenotype
  • abnormal class switch recombination
    • specific IgG antibodies are almost undetectable in SRBC immunized mutants, indicating defective class switching   (MGI Ref ID J:80616)
  • abnormal spleen morphology
    • spleen architecture is severely disturbed   (MGI Ref ID J:80616)
    • distribution of lymphocytes and their functional compartmentalization are severely disorganized in the spleen, with IgD+ B cells scattered along red and white pulp and T cells mostly condensed around central arterioles   (MGI Ref ID J:80616)
    • abnormal spleen B cell follicle morphology
      • absence of polarized B-cell follicles   (MGI Ref ID J:80616)
      • absent spleen germinal center
        • do not form germinal centers   (MGI Ref ID J:80616)
    • absent spleen marginal zone   (MGI Ref ID J:80616)
    • decreased spleen white pulp amount
      • gradual reduction of white pulp size   (MGI Ref ID J:80616)
  • absent Peyer's patches   (MGI Ref ID J:80616)
  • absent axillary lymph nodes   (MGI Ref ID J:80616)
  • absent brachial lymph nodes   (MGI Ref ID J:80616)
  • absent inguinal lymph nodes   (MGI Ref ID J:80616)
  • absent lymph nodes   (MGI Ref ID J:80616)
  • absent mesenteric lymph nodes   (MGI Ref ID J:80616)
  • absent popliteal lymph nodes   (MGI Ref ID J:80616)
  • decreased IgG level
    • specific IgG antibodies are almost undetectable in SRBC immunized mutants   (MGI Ref ID J:80616)
  • increased leukocyte cell number
    • 2-3 fold increase in the number of leukocytes in the spleen, blood, and peritoneal cavity   (MGI Ref ID J:80616)
  • increased susceptibility to bacterial infection
    • increased susceptibility to Listeria monocytogenes infections, with mutants dying by day 8 while wild-type mice survive   (MGI Ref ID J:80616)
    • increased susceptibility to Salmonella enterica infection   (MGI Ref ID J:80616)
  • hematopoietic system phenotype
  • abnormal class switch recombination
    • specific IgG antibodies are almost undetectable in SRBC immunized mutants, indicating defective class switching   (MGI Ref ID J:80616)
  • abnormal spleen morphology
    • spleen architecture is severely disturbed   (MGI Ref ID J:80616)
    • distribution of lymphocytes and their functional compartmentalization are severely disorganized in the spleen, with IgD+ B cells scattered along red and white pulp and T cells mostly condensed around central arterioles   (MGI Ref ID J:80616)
    • abnormal spleen B cell follicle morphology
      • absence of polarized B-cell follicles   (MGI Ref ID J:80616)
      • absent spleen germinal center
        • do not form germinal centers   (MGI Ref ID J:80616)
    • absent spleen marginal zone   (MGI Ref ID J:80616)
    • decreased spleen white pulp amount
      • gradual reduction of white pulp size   (MGI Ref ID J:80616)
  • decreased IgG level
    • specific IgG antibodies are almost undetectable in SRBC immunized mutants   (MGI Ref ID J:80616)
  • increased leukocyte cell number
    • 2-3 fold increase in the number of leukocytes in the spleen, blood, and peritoneal cavity   (MGI Ref ID J:80616)

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Ltb/Tnf/Ltatm1Dvk
Allele Name targeted mutation 1, Dmitry V Kuprash
Allele Type Targeted (Null/Knockout)
Common Name(s) TNF/LTDelta3; Tnfabc-;
Mutation Made By Dmitry Kuprash,   Engelhardt Inst of Molecular Biology
Gene Symbol and Name Lta, lymphotoxin A
Chromosome 17
Gene Common Name(s) LT; LT-[a]; LT-alpha; LT[a]; LTalpha; Ltx; TNF beta; TNF-beta; TNFB; TNFSF1; Tnfb; Tnfsf1b; heart, lung and blood 382; hlb382; lymphotoxin; lymphotoxin alpha; tumor necrosis factor beta;
Molecular Note An 8.4 kb region, containing the entire Tnf gene and the last coding exons of the Lta and Ltb genes, was deleted by cre mediated recombination of surrounding loxP sites inserted within Lta and Ltb via homologous recombiantion. Partial coding regions of Lta and Ltb were left intact separtated by a single loxP site, however RT-PCR analysis of homozygous mutant splenocytes activated by concanavalin A indicated an absence of transcript from each locus. [MGI Ref ID J:80616]
 
Gene Symbol and Name Tnf, tumor necrosis factor
Chromosome 17
Gene Common Name(s) DIF; TNF alpha; TNF-alpha; TNFA; TNFSF2; TNFalpha; Tnfa; Tnfsf1a; tumor necrosis factor, alpha; tumor necrosis factor-alpha;
 
Gene Symbol and Name Ltb, lymphotoxin B
Chromosome 17
Gene Common Name(s) AI662801; LTbeta; TNFC; TNFSF3; Tnfc; expressed sequence AI662801; lymphotoxin beta; p33; tumor necrosis factor C;

Genotyping

Genotyping Information

Genotyping Protocols

Ltb/Tnf/Ltatm1Dvk, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Kuprash DV; Alimzhanov MB; Tumanov AV; Grivennikov SI; Shakhov AN; Drutskaya LN; Marino MW; Turetskaya RL; Anderson AO; Rajewsky K; Pfeffer K; Nedospasov SA. 2002. Redundancy in tumor necrosis factor (TNF) and lymphotoxin (LT) signaling in vivo: mice with inactivation of the entire TNF/LT locus versus single-knockout mice. Mol Cell Biol 22(24):8626-34. [PubMed: 12446781]  [MGI Ref ID J:80616]

Additional References

Ltb/Tnf/Ltatm1Dvk related

Baseta JG; Stutman O. 2000. TNF regulates thymocyte production by apoptosis and proliferation of the triple negative (CD3-CD4-CD8-) subset. J Immunol 165(10):5621-30. [PubMed: 11067918]  [MGI Ref ID J:112282]

Chen X; Wu X; Zhou Q; Howard OM; Netea MG; Oppenheim JJ. 2013. TNFR2 is critical for the stabilization of the CD4+Foxp3+ regulatory T. cell phenotype in the inflammatory environment. J Immunol 190(3):1076-84. [PubMed: 23277487]  [MGI Ref ID J:193029]

Kendall GS; Hirstova M; Horn S; Dafou D; Acosta-Saltos A; Almolda B; Zbarsky V; Rumajogee P; Heuer H; Castellano B; Pfeffer K; Nedospasov SA; Peebles DM; Raivich G. 2011. TNF gene cluster deletion abolishes lipopolysaccharide-mediated sensitization of the neonatal brain to hypoxic ischemic insult. Lab Invest 91(3):328-41. [PubMed: 21135813]  [MGI Ref ID J:169257]

Liepinsh DJ; Grivennikov SI; Klarmann KD; Lagarkova MA; Drutskaya MS; Lockett SJ; Tessarollo L; McAuliffe M; Keller JR; Kuprash DV; Nedospasov SA. 2006. Novel lymphotoxin alpha (LTalpha) knockout mice with unperturbed tumor necrosis factor expression: reassessing LTalpha biological functions. Mol Cell Biol 26(11):4214-25. [PubMed: 16705172]  [MGI Ref ID J:109621]

Maddox DM; Hicks WL; Vollrath D; LaVail MM; Naggert JK; Nishina PM. 2011. An ENU-induced mutation in the Mertk gene (Mertknmf12) leads to a slow form of retinal degeneration. Invest Ophthalmol Vis Sci 52(7):4703-9. [PubMed: 21436282]  [MGI Ref ID J:175587]

Mouchess ML; Arpaia N; Souza G; Barbalat R; Ewald SE; Lau L; Barton GM. 2011. Transmembrane Mutations in Toll-like Receptor 9 Bypass the Requirement for Ectodomain Proteolysis and Induce Fatal Inflammation. Immunity 35(5):721-32. [PubMed: 22078797]  [MGI Ref ID J:178832]

Rebholz B; Haase I; Eckelt B; Paxian S; Flaig MJ; Ghoreschi K; Nedospasov SA; Mailhammer R; Debey-Pascher S; Schultze JL; Weindl G; Forster I; Huss R; Stratis A; Ruzicka T; Rocken M; Pfeffer K; Schmid RM; Rupec RA. 2007. Crosstalk between Keratinocytes and Adaptive Immune Cells in an IkappaBalpha Protein-Mediated Inflammatory Disease of the Skin. Immunity 27(2):296-307. [PubMed: 17692539]  [MGI Ref ID J:124343]

Tumanov AV; Kuprash DV; Mach JA; Nedospasov SA; Chervonsky AV. 2004. Lymphotoxin and TNF produced by B cells are dispensable for maintenance of the follicle-associated epithelium but are required for development of lymphoid follicles in the Peyer's patches. J Immunol 173(1):86-91. [PubMed: 15210762]  [MGI Ref ID J:90815]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryThese mice are considered immunocompromised; SPF conditions are recommended.
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $1650.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $2145.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(6.8)