Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote         (Female x Male) Species laboratory mouse Generation N11F?+F9 (04-DEC-07)   Donating Investigator William Parks,   University of Washington

Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA) is detected by Northern blot analysis of the small intestine. Immunohistochemical analysis of intestinal tissue from homozygotes does not detect the gene product (protein) in Paneth cells. Mutant mice have impaired innate host defense response due to the lack of mature crypt defensin proteins in intestinal epithelium. These mice are more susceptible to bacterial infection of the small intestine mucosal epithelium. Wound repair (reepithelialization) and neutrophil infiltration following respiratory airway injury is defective. Apoptosis is reduced in prostate tissue following castration, and in pancreatic acinar cells following pancreatic duct ligation. This mutant mouse strain may be useful in studies related to intestinal and pancreatic tumorigenesis, epithelial wound repair, inflammation and mucosal immune response.

Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt 95 nucleotides in exon 4. The construct was electroporated into 129 derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to the same for 11 generations (January 2004). Heterozygotes were bred to generate homozygotes.

Control Information

	Control
	000664 C57BL/6J	(approximate)
Considerations for Choosing Controls

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Mmp7^tm1Lmm/Mmp7^tm1Lmm

        involves: 129S1/Sv * 129X1/SvJ * C57BL/6

• normal phenotype
• no abnormal phenotype detected (MGI Ref ID J:38609)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Immunology and Inflammation Research
Inflammation (Neutrophil defects)

Internal/Organ Research
Wound Healing (delayed/impaired)

Genes & Alleles

Gene & Allele Information

Allele Symbol		Mmp7tm1Lmm
Allele Name		targeted mutation 1, Lynn M Matrisian
Allele Type		Targeted (knock-out)
Common Name(s)		MAT-; Mmp7tm1vu; mmp7-;
Mutation Made By		Carole Wilson and Lynn Matrisian,   Univ. Washington School of Medicine
Strain of Origin		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line Name		R1
ES Cell Line Strain		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name		Mmp7, matrix metallopeptidase 7
Chromosome		9
Gene Common Name(s)		MAT; MMP-7; MPMM; MPSL1; PUMP-1; matrilysin;
Molecular Note		A neomycin resistance cassette replaced approximately 95 nucleotides of coding sequence in exon 4 of the gene. No transcript for the targeted gene was detectable by Northern blot analysis of RNA from the small intestine of homozygous mutant mice. [MGI Ref ID J:38609]

Genotyping

Genotyping Information

Genotyping Protocols

Mmp7^tm1Lmm, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Wilson CL; Heppner KJ; Labosky PA; Hogan BL; Matrisian LM. 1997. Intestinal tumorigenesis is suppressed in mice lacking the metalloproteinase matrilysin. Proc Natl Acad Sci U S A 94(4):1402-7. [PubMed: 9037065]  [MGI Ref ID J:38609]

Additional References

Li Q; Park PW; Wilson CL; Parks WC. 2002. Matrilysin shedding of syndecan-1 regulates chemokine mobilization and transepithelial efflux of neutrophils in acute lung injury. Cell 111(5):635-46. [PubMed: 12464176]  [MGI Ref ID J:80692]

Mmp7^tm1Lmm related

Acuff HB; Carter KJ; Fingleton B; Gorden DL; Matrisian LM. 2006. Matrix metalloproteinase-9 from bone marrow-derived cells contributes to survival but not growth of tumor cells in the lung microenvironment. Cancer Res 66(1):259-66. [PubMed: 16397239]  [MGI Ref ID J:105098]

Betsuyaku T; Fukuda Y; Parks WC; Shipley JM; Senior RM. 2000. Gelatinase B is required for alveolar bronchiolization after intratracheal bleomycin. Am J Pathol 157(2):525-35. [PubMed: 10934155]  [MGI Ref ID J:108171]

Chen P; McGuire JK; Hackman RC; Kim KH; Black RA; Poindexter K; Yan W; Liu P; Chen AJ; Parks WC; Madtes DK. 2008. Tissue inhibitor of metalloproteinase-1 moderates airway re-epithelialization by regulating matrilysin activity. Am J Pathol 172(5):1256-70. [PubMed: 18385523]  [MGI Ref ID J:134273]

Crawford HC; Scoggins CR; Washington MK; Matrisian LM; Leach SD. 2002. Matrix metalloproteinase-7 is expressed by pancreatic cancer precursors and regulates acinar-to-ductal metaplasia in exocrine pancreas. J Clin Invest 109(11):1437-44. [PubMed: 12045257]  [MGI Ref ID J:76811]

Dunsmore SE; Saarialho-Kere UK; Roby JD; Wilson CL; Matrisian LM; Welgus HG; Parks WC. 1998. Matrilysin expression and function in airway epithelium. J Clin Invest 102(7):1321-31. [PubMed: 9769324]  [MGI Ref ID J:115243]

Fujino RS; Ishikawa Y; Tanaka K; Kanatsu-Shinohara M; Tamura K; Kogo H; Shinohara T; Hara T. 2006. Capillary morphogenesis gene (CMG)-1 is among the genes differentially expressed in mouse male germ line stem cells and embryonic stem cells. Mol Reprod Dev 73(8):955-66. [PubMed: 16705683]  [MGI Ref ID J:119227]

Guillen-Ahlers H; Buechler SA; Suckow MA; Castellino FJ; Ploplis VA. 2008. Sulindac treatment alters collagen and matrilysin expression in adenomas of ApcMin/+ mice. Carcinogenesis 29(7):1421-7. [PubMed: 18499699]  [MGI Ref ID J:139063]

Haro H; Crawford HC; Fingleton B; Shinomiya K; Spengler DM; Matrisian LM. 2000. Matrix metalloproteinase-7-dependent release of tumor necrosis factor-alpha in a model of herniated disc resorption. J Clin Invest 105(2):143-50. [PubMed: 10642592]  [MGI Ref ID J:124841]

Hulboy DL; Gautam S; Fingleton B; Matrisian LM. 2004. The influence of matrix metalloproteinase-7 on early mammary tumorigenesis in the multiple intestinal neoplasia mouse. Oncol Rep 12(1):13-7. [PubMed: 15201952]  [MGI Ref ID J:91647]

Kure T; Chang JH; Kato T; Hernandez-Quintela E; Ye H; Lu PC; Matrisian LM; Gatinel D; Shapiro S; Gosheh F; Azar DT. 2003. Corneal neovascularization after excimer keratectomy wounds in matrilysin-deficient mice. Invest Ophthalmol Vis Sci 44(1):137-44. [PubMed: 12506066]  [MGI Ref ID J:119224]

Lee MM; Yoon BJ; Osiewicz K; Preston M; Bundy B; van Heeckeren AM; Werb Z; Soloway PD. 2005. Tissue inhibitor of metalloproteinase 1 regulates resistance to infection. Infect Immun 73(1):661-5. [PubMed: 15618213]  [MGI Ref ID J:94836]

Li Q; Park PW; Wilson CL; Parks WC. 2002. Matrilysin shedding of syndecan-1 regulates chemokine mobilization and transepithelial efflux of neutrophils in acute lung injury. Cell 111(5):635-46. [PubMed: 12464176]  [MGI Ref ID J:80692]

Lindsey ML; Escobar GP; Mukherjee R; Goshorn DK; Sheats NJ; Bruce JA; Mains IM; Hendrick JK; Hewett KW; Gourdie RG; Matrisian LM; Spinale FG. 2006. Matrix metalloproteinase-7 affects connexin-43 levels, electrical conduction, and survival after myocardial infarction. Circulation 113(25):2919-28. [PubMed: 16769909]  [MGI Ref ID J:122893]

Martin MD; Carter KJ; Jean-Philippe SR; Chang M; Mobashery S; Thiolloy S; Lynch CC; Matrisian LM; Fingleton B. 2008. Effect of ablation or inhibition of stromal matrix metalloproteinase-9 on lung metastasis in a breast cancer model is dependent on genetic background. Cancer Res 68(15):6251-9. [PubMed: 18676849]  [MGI Ref ID J:140033]

Pal S; Schmidt AP; Peterson EM; Wilson CL; de la Maza LM. 2006. Role of matrix metalloproteinase-7 in the modulation of a Chlamydia trachomatis infection. Immunology 117(2):213-9. [PubMed: 16423057]  [MGI Ref ID J:106779]

Reil JC; Gilles S; Zahler S; Brandl A; Drexler H; Hultner L; Matrisian LM; Welsch U; Becker BF. 2007. Insights from knock-out models concerning postischemic release of TNFalpha from isolated mouse hearts. J Mol Cell Cardiol 42(1):133-41. [PubMed: 17101148]  [MGI Ref ID J:119661]

Rudolph-Owen LA; Hulboy DL; Wilson CL; Mudgett J; Matrisian LM. 1997. Coordinate expression of matrix metalloproteinase family members in the uterus of normal, matrilysin-deficient, and stromelysin-1-deficient mice. Endocrinology 138(11):4902-11. [PubMed: 9348221]  [MGI Ref ID J:43855]

Swee M; Wilson CL; Wang Y; McGuire JK; Parks WC. 2008. Matrix metalloproteinase-7 (matrilysin) controls neutrophil egress by generating chemokine gradients. J Leukoc Biol 83(6):1404-12. [PubMed: 18334539]  [MGI Ref ID J:136866]

Takahashi N; Vanlaere I; de Rycke R; Cauwels A; Joosten LA; Lubberts E; van den Berg WB; Libert C. 2008. IL-17 produced by Paneth cells drives TNF-induced shock. J Exp Med 205(8):1755-61. [PubMed: 18663129]  [MGI Ref ID J:139519]

Wang M; Qin X; Mudgett JS; Ferguson TA; Senior RM; Welgus HG. 1999. Matrix metalloproteinase deficiencies affect contact hypersensitivity: stromelysin-1 deficiency prevents the response and gelatinase B deficiency prolongs the response. Proc Natl Acad Sci U S A 96(12):6885-9. [PubMed: 10359808]  [MGI Ref ID J:55723]

Xu J; Park PW; Kheradmand F; Corry DB. 2005. Endogenous attenuation of allergic lung inflammation by syndecan-1. J Immunol 174(9):5758-65. [PubMed: 15843578]  [MGI Ref ID J:98401]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Mating System	Homozygote x Homozygote         (Female x Male)
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.

Supply Notes

Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	000664 C57BL/6J	(approximate)
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

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In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.