Strain Name: |
NOD.FVB-Tg(Ins1-Cat,Tyr)25Pne/PneJ |
|---|---|
Stock Number: |
005114 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Former Name |
NOD.FVB-Tg(Ins1-Cat, Tyr)25Pne/PneJ (Changed: 15-DEC-04
) |
| Genes & Alleles | Cat; Ins1; Tyr; Tg(Ins1-Cat,Tyr)25Pne; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Background Strain NOD Donor Strain FVB Donating Investigator Paul Epstein, University of Louisville H2 Haplotype g7 Strain Description
Transgenic mice are viable, fertile, and normal in size, display accelerated spontaneous diabetes onset in males, and have coat color pigmentation. Northern blot analysis and immunohistochemistry confirm pancreatic islet specific expression of the Ins1-Cat transgene. There is a 50-fold increase in catalase activity in transgenic islets when compared to wild-type controls, which approximates catalase activity normally found in the liver. Insulin and DNA content, insulin staining and islet morphology in transgenic animals is essentially the same as in wild-type controls.Transgenic islets have a similar reactive oxygen species (ROS) level as wild-type controls in normal media. However, after exposure to cytokines there is a significant increase of ROS florescence in control islets, but much smaller increase in transgenic islets. Transgenic mice are resistant to diabetes when treated with streptozotocin or alloxan. Transgenic beta cells generate less ROS fluorescence when treated with hydrogen peroxide, superoxide or peroxynitrite, but surprisingly cyclophosphamide accelerates diabetes onset in transgenic mice.
This model provides a tool for looking at the role of oxidative stress in diabetes.
Strain Development
NOD.FVB-Tg(Ins1-Cat, Tyr)25Pne/PneJ expresses the full-length rat catalase cDNA under the control of the rat insulin 1 promoter. In addition, these mice express tyrosinase under the control of the tyrosinase promoter, commonly referred to as TyBs or tyrosinase minigene (Xu et al, 1999, Overbeek et al., 1991). These transgenes were first co-inserted by Xu et al., (1999) into FVB oocytes. Transgenic founders carrying both genes were mated to FVB and found to co-segregate. This strain was maintained by Epstein et al., and backcrossed to NOD for 8 generations. A genome wide scan confirmed that all known Idd markers were homozygous for the NOD allele. In 2004, The Jackson Laboratory received NOD.FVB-Tg(Ins1-Cat,Tyr)25Pne/PneJ at generation N8F7.
Related Disease (OMIM) Terms |
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(Ins1-Cat,Tyr)25Pne | ||
|---|---|---|---|
| Allele Name | transgene insertion 25, Paul N Epstein | ||
| Common Name(s) | RipCat; | ||
| Mutation Made By | Paul Epstein, University of Louisville | ||
| Strain of Origin | FVB | ||
| Expressed Gene | Tyr, tyrosinase, mouse, laboratory | ||
| Expressed Gene | Cat, catalase, rat | ||
| Promoter | Ins1, insulin 1, rat | ||
| Molecular Note | The symbol represents a double transgene. In one transgene, a rat insulin I promoter drives the expression of a rat catalase cDNA. Northern blot analysis and immunohistochemistry detected expression of this transgene in pancreatic beta cells. A second transgene that expresses tyrosinase under control of the tyrosinase promoter was coinjected with the first transgene, and apparently cosegregated. [MGI Ref ID J:59725] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001976 NOD/ShiLtJ | ||
| Considerations for Choosing Controls | ||
Colony Maintenance
| Diet Information | LabDiet® 5K52/5K67 |
|---|
Related Strains
Strains carrying other alleles of Ins1
005282 NOD/ShiLtJ-Tg(Ins1-EGFP/GH1)14Hara/HaraJ View Strains carrying other alleles of Ins1 (1 strain)
Additional Web Information
Genetic Quality Control Annual Report
Research Applications
This mouse can be used to support research in many areas including:
Diabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains (NOD Transgenics)
Immunology and Inflammation Research
Autoimmunity (Type 1 Diabetes)
Research Tools
Apoptosis Research
References
Selected Reference(s)
Additional ReferencesXu B; Moritz JT; Epstein PN. 1999. Overexpression of catalase provides partial protection to transgenic mouse beta cells. Free Radic Biol Med 27(7-8):830-7. [PubMed: 10515587] [MGI Ref ID J:59725]
Price and Supply Information
| Strain Name: | NOD.FVB-Tg(Ins1-Cat,Tyr)25Pne/PneJ |
| Stock Number: | 005114 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
*NO Shipping Destination selected!
Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form