Strain Name: |
FVB.129S6(B6)-Gt(ROSA)26Sortm1(Luc)Kael/J |
|---|---|
Stock Number: |
005125 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Gt(ROSA)26Sor; Gt(ROSA)26Sortm1(Luc)Kael; luc; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator William Kaelin, Dana Farber Cancer Institute Generation N4+2F2 (06-DEC-06) Strain Description
These mice contain the firefly luciferase (luc) gene inserted into the Gt(ROSA)26Sor locus. Expression of the luciferase gene is blocked by a loxP-flanked STOP fragment placed between the luc sequence and the Gt(ROSA)26Sor promoter. This strain serves as a Cre reporter strain. When used in conjunction with a Cre recombinase-expressing strain, successful Cre-mediated excision is indicated by luciferase expression in Cre-expressing tissues. Mice that are heterozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.Strain Development
A targeting vector containing a PGK-neo cassette and a loxP-stop-loxP cassette upstream of the firefly luciferase (luc) gene was inserted into the Gt(ROSA)26Sor locus. The construct was introduced into 129S6/SvEvTac-derived TC-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were bred to FVB mice and backcrossed for 4 generations on the FVB background.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Gt(ROSA)26Sortm1(Luc)Kael | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, William G Kaelin | ||
| Common Name(s) | ROSA26 L-S-L-Luc; | ||
| Mutation Made By | Michal Safran, Dana Farber Cancer Institute | ||
| Strain of Origin | 129S6/SvEvTac | ||
| ES Cell Line Name | TC-1 | ||
| ES Cell Line Strain | 129S6/SvEvTac | ||
| Site of Expression | when crossed to a cre recombinase-expressing strain, luciferase expression is observed in the cre-expressing tissues | ||
| Expressed Gene | luc, luciferase, firefly | ||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
| Molecular Note | The endogenous locus is interrupted by a construct comprising a loxP site, a neomycin resistance cassette with a strong, triple SV40 polyadenylation signal, and a second loxP site in the same orientation as the first followed by the cDNA sequence encoding firefly luciferase and the bovine growth hormone polyadenylation signal. In cells in which Cre recombinase is expressed, the DNA between the loxP sites is excised, along with one loxP site, permitting expression of the luciferase gene under control of the ubiquitous ROSA26 promoter. [MGI Ref ID J:92043] | ||
Control Information
| Allele | Control | |
|---|---|---|
| Gt(ROSA)26Sortm1(Luc)Kael | 001800 FVB/NJ | |
| Considerations for Choosing Controls | ||
Genotyping Protocols
Gt(ROSA)26Sortm1(Luc)Kael
Colony Maintenance
| Breeding & Husbandry | This strain is maintained as a homozygote. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Fluorescent Protein Strains
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Additional Web Information
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
Fluorescent Proteins/lacZ Systems
Genetic Quality Control Annual Report
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
Research Tools
Cre-lox System (loxP-flanked Sequences: Test/Reporter)
Developmental Biology Research (Cre-lox System)
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers: Cre-lox System)
References
Selected Reference(s)
Safran M; Kim WY; Kung AL; Horner JW; DePinho RA; Kaelin WG Jr. 2003. Mouse reporter strain for noninvasive bioluminescent imaging of cells that have undergone Cre-mediated recombination. Mol Imaging 2(4):297-302. [PubMed: 14717328] [MGI Ref ID J:92043]
Price and Supply Information
| Strain Name: | FVB.129S6(B6)-Gt(ROSA)26Sortm1(Luc)Kael/J |
| Stock Number: | 005125 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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