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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote (Female x Male) 01-MAR-06 Species laboratory mouse Generation N4+2F9 (24-MAR-11)
Generation DefinitionsDonating Investigator William Kaelin, Dana Farber Cancer Institute Description
These mice contain the firefly luciferase (luc) gene inserted into the Gt(ROSA)26Sor locus. Expression of the luciferase gene is blocked by a loxP-flanked STOP fragment placed between the luc sequence and the Gt(ROSA)26Sor promoter. This strain serves as a Cre reporter strain. When used in conjunction with a Cre recombinase-expressing strain, successful Cre-mediated excision is indicated by luciferase expression in Cre-expressing tissues. Mice that are heterozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.Development
A targeting vector containing a PGK-neo cassette and a loxP-stop-loxP cassette upstream of the firefly luciferase (luc) gene was inserted into the Gt(ROSA)26Sor locus. The construct was introduced into 129S6/SvEvTac-derived TC-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were bred to FVB mice and backcrossed for 4 generations on the FVB background.
Control Information
| Control | ||
|---|---|---|
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (345 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Genetic Quality Control Annual Report
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Gt(ROSA)26Sortm1(Luc)Kael/Gt(ROSA)26Sor+
involves: 129S6/SvEvTac * FVB/NJ
- normal phenotype
- no abnormal phenotype detected
- mice heterozygous for this targeted reporter gene are viable, fertile and exhibit no gross physical or behavioral abnormalities (MGI Ref ID J:92043)
This mouse can be used to support research in many areas including:
Research Tools
Cre-lox System
loxP-flanked Sequences
loxP-flanked Sequences: Test/Reporter
Developmental Biology Research
Cre-lox System
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers
Tissue/Cell Markers: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Gt(ROSA)26Sortm1(Luc)Kael | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, William G Kaelin | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | ROSA26 L-S-L-Luc; | ||
| Mutation Made By | Michal Safran, Dana Farber Cancer Institute | ||
| Strain of Origin | 129S6/SvEvTac | ||
| ES Cell Line Name | TC1/TC-1 | ||
| ES Cell Line Strain | 129S6/SvEvTac | ||
| Site of Expression | when crossed to a cre recombinase-expressing strain, luciferase expression is observed in the cre-expressing tissues | ||
| Expressed Gene | luc, luciferase, firefly | ||
| Molecular Note | The endogenous locus is interrupted by a construct comprising a loxP site, a neomycin resistance cassette with a strong, triple SV40 polyadenylation signal, and a second loxP site in the same orientation as the first followed by the cDNA sequence encoding firefly luciferase and the bovine growth hormone polyadenylation signal. In cells in which Cre recombinase is expressed, the DNA between the loxP sites is excised, along with one loxP site, permitting expression of the luciferase gene under control of the ubiquitous ROSA26 promoter. [MGI Ref ID J:92043] | ||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gt(ROSA)26Sortm1(Luc)Kael, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Safran M; Kim WY; Kung AL; Horner JW; DePinho RA; Kaelin WG Jr. 2003. Mouse reporter strain for noninvasive bioluminescent imaging of cells that have undergone Cre-mediated recombination. Mol Imaging 2(4):297-302. [PubMed: 14717328] [MGI Ref ID J:92043]
Additional References
Gt(ROSA)26Sortm1(Luc)Kael relatedDorner M; Horwitz JA; Robbins JB; Barry WT; Feng Q; Mu K; Jones CT; Schoggins JW; Catanese MT; Burton DR; Law M; Rice CM; Ploss A. 2011. A genetically humanized mouse model for hepatitis C virus infection. Nature 474(7350):208-11. [PubMed: 21654804] [MGI Ref ID J:173453]
Li Z; Tognon CE; Godinho FJ; Yasaitis L; Hock H; Herschkowitz JI; Lannon CL; Cho E; Kim SJ; Bronson RT; Perou CM; Sorensen PH; Orkin SH. 2007. ETV6-NTRK3 fusion oncogene initiates breast cancer from committed mammary progenitors via activation of AP1 complex. Cancer Cell 12(6):542-58. [PubMed: 18068631] [MGI Ref ID J:130323]
Premsrirut PK; Dow LE; Kim SY; Camiolo M; Malone CD; Miething C; Scuoppo C; Zuber J; Dickins RA; Kogan SC; Shroyer KR; Sordella R; Hannon GJ; Lowe SW. 2011. A rapid and scalable system for studying gene function in mice using conditional RNA interference. Cell 145(1):145-58. [PubMed: 21458673] [MGI Ref ID J:171191]
Svensson RU; Haverkamp JM; Thedens DR; Cohen MB; Ratliff TL; Henry MD. 2011. Slow disease progression in a C57BL/6 pten-deficient mouse model of prostate cancer. Am J Pathol 179(1):502-12. [PubMed: 21703427] [MGI Ref ID J:173997]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry This strain is maintained as a homozygote. Mating System Homozygote x Homozygote (Female x Male) 01-MAR-06 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $172.00 Female or Male Homozygous for Gt(ROSA)26Sortm1(Luc)Kael
Pairs /Price (US dollars $) Pair Genotype $344.00 Homozygous for Gt(ROSA)26Sortm1(Luc)Kael x Homozygous for Gt(ROSA)26Sortm1(Luc)Kael Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $223.60 Female or Male Homozygous for Gt(ROSA)26Sortm1(Luc)Kael
Pairs /Price (US dollars $) Pair Genotype $447.20 Homozygous for Gt(ROSA)26Sortm1(Luc)Kael x Homozygous for Gt(ROSA)26Sortm1(Luc)Kael Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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