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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names B6.Cg-Sema7atm1Alk/J (Changed: 06-SEP-06 ) Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N7p (15-MAY-05)
Generation DefinitionsDonating Investigator Alex Kolodkin, Johns Hopkins Univ Schl of Medicine Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA) is detected by Northern blot analysis. By age embryonic day 16, homozygotes exhibit abnormal lateral olfactory tract outgrowth. The lateral olfactory tract (LOT) from mutants have a more narrow morphology when compared to wildtype controls. In many mutants, no LOT is detected in the most caudal regions. This mutant mouse strain may be useful in studies of lateral olfactory tract development and axonal growth.Development
A loxP- and FRT-flanked targeting vector containing a neomycin resistance gene driven by the mouse phosphoglycerate kinase promoter, exon 1 and 1.1kb of flanking sequence, was utilized in the construction of this mutant. The construct was electroporated into 129S6/SvEvTac derived MC1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric male mice were crossed to C57BL/6 mice, and then crossed to cre deleter strain, BALB/c-Tg(CMV-cre)1Cgn/J (STOCK#3465) to remove the floxed targeting vector. The mice were then backcrossed onto C57BL/6 for 6 generations. The Donating Investigator reports that it is possible that an additional cross to a non-C57BL/6 strain prior to the strain's shipment to The Jackson Laboratory may have occurred. This is consistnet with the observation of agouti pups among the offspring.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
Phenotype
Phenotype Information
View Related Disease (OMIM) Terms
Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Blood Group, John Milton Hagen System (SEMA7A)
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Sema7atm1Alk/Sema7atm1Alk
involves: 129S6/SvEvTac * C57BL/6
- nervous system phenotype
- abnormal axon outgrowth
- retrograde DiI axon tracing in coronal brain sections indicates that the overall LOT projection is either significantly reduced in size or entirely absent at the most caudal levels, suggesting that most OB axons fail to project into the most caudal parts of the olfactory cortex by E16 (MGI Ref ID J:87191)
- in vivo, branching of the LOT appears to be reduced probably due to an overall outgrowth impairment; however, no effect on OB axon branching is observed in vitro or in situ (MGI Ref ID J:87191)
- abnormal olfactory tract morphology
- at E16, homozygotes display impaired lateral olfactory tract (LOT) outgrowth relative to wild-type and heterozygous control littermates (MGI Ref ID J:87191)
- an abnormally narrow LOT morphology is observed in E16 whole-mount mutant brains (MGI Ref ID J:87191)
- most olfactory bulb (OB) axons fail to project into the most caudal parts of the olfactory cortex by E16, as shown by retrograde DiI axon tracing in coronal brain sections (MGI Ref ID J:87191)
- however, both OB cytoarchitecture and mitral/tufted cell numbers are normal, and tract positioning in the telencephalon remains unaffected (MGI Ref ID J:87191)
- cellular phenotype
- abnormal axon outgrowth
- retrograde DiI axon tracing in coronal brain sections indicates that the overall LOT projection is either significantly reduced in size or entirely absent at the most caudal levels, suggesting that most OB axons fail to project into the most caudal parts of the olfactory cortex by E16 (MGI Ref ID J:87191)
- in vivo, branching of the LOT appears to be reduced probably due to an overall outgrowth impairment; however, no effect on OB axon branching is observed in vitro or in situ (MGI Ref ID J:87191)
This mouse can be used to support research in many areas including:Sema7atm1Alk related
Neurobiology Research
Neurodevelopmental Defects
Sensorineural Research
Olfactory Defects
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Sema7atm1Alk | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Alex L Kolodkin | ||
| Allele Type | Targeted (knock-out) | ||
| Common Name(s) | Sema7a-; null- SEMA 7A; | ||
| Mutation Made By | R. Pasterkamp, Rudolf Magnus Institute of Neuroscience | ||
| Strain of Origin | 129S6/SvEvTac | ||
| ES Cell Line Name | MC1 | ||
| ES Cell Line Strain | 129S6/SvEvTac | ||
| Gene Symbol and Name | Sema7a, sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A | ||
| Chromosome | 9 | ||
| Gene Common Name(s) | 2900057C09Rik; CD108; CDw108; H-SEMA-K1; H-Sema-L; JMH; RIKEN cDNA 2900057C09 gene; SEMAK1; SEMAL; Semal; Semaphorin K1; semaphorin L; | ||
| Molecular Note | A single loxP site remained in place exon 1 as well as 1.1 kb of upstream DNA. The deleted exon includes the start codon and encodes 59 residues which comprise the entire signal sequence. [MGI Ref ID J:87191] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Sema7atm1Alk, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Pasterkamp RJ; Peschon JJ; Spriggs MK; Kolodkin AL. 2003. Semaphorin 7A promotes axon outgrowth through integrins and MAPKs. Nature 424(6947):398-405. [PubMed: 12879062] [MGI Ref ID J:87191]
Additional References
Sema7atm1Alk relatedCzopik AK; Bynoe MS; Palm N; Raine CS; Medzhitov R. 2006. Semaphorin 7A is a negative regulator of T cell responses. Immunity 24(5):591-600. [PubMed: 16713976] [MGI Ref ID J:113360]
Ishii H; Kubo T; Kumanogoh A; Yamashita T. 2010. Th1 cells promote neurite outgrowth from cortical neurons via a mechanism dependent on semaphorins. Biochem Biophys Res Commun 402(1):168-72. [PubMed: 20946887] [MGI Ref ID J:166064]
Kang HR; Lee CG; Homer RJ; Elias JA. 2007. Semaphorin 7A plays a critical role in TGF-beta1-induced pulmonary fibrosis. J Exp Med 204(5):1083-93. [PubMed: 17485510] [MGI Ref ID J:125730]
Kang S; Okuno T; Takegahara N; Takamatsu H; Nojima S; Kimura T; Yoshida Y; Ito D; Ohmae S; You DJ; Toyofuku T; Jang MH; Kumanogoh A. 2012. Intestinal epithelial cell-derived semaphorin 7A negatively regulates development of colitis via alphavbeta1 integrin. J Immunol 188(3):1108-16. [PubMed: 22198947] [MGI Ref ID J:180764]
Messina A; Ferraris N; Wray S; Cagnoni G; Donohue DE; Casoni F; Kramer PR; Derijck AA; Adolfs Y; Fasolo A; Pasterkamp RJ; Giacobini P. 2011. Dysregulation of Semaphorin7A/{beta}1-integrin signaling leads to defective GnRH-1 cell migration, abnormal gonadal development and altered fertility. Hum Mol Genet :. [PubMed: 21903667] [MGI Ref ID J:177773]
Morote-Garcia JC; Napiwotzky D; Kohler D; Rosenberger P. 2012. Endothelial Semaphorin 7A promotes neutrophil migration during hypoxia. Proc Natl Acad Sci U S A 109(35):14146-51. [PubMed: 22891341] [MGI Ref ID J:188572]
Sultana H; Neelakanta G; Foellmer HG; Montgomery RR; Anderson JF; Koski RA; Medzhitov RM; Fikrig E. 2012. Semaphorin 7A contributes to West Nile virus pathogenesis through TGF-beta1/Smad6 signaling. J Immunol 189(6):3150-8. [PubMed: 22896629] [MGI Ref ID J:189946]
Suzuki K; Okuno T; Yamamoto M; Pasterkamp RJ; Takegahara N; Takamatsu H; Kitao T; Takagi J; Rennert PD; Kolodkin AL; Kumanogoh A; Kikutani H. 2007. Semaphorin 7A initiates T-cell-mediated inflammatory responses through alpha1beta1 integrin. Nature 446(7136):680-4. [PubMed: 17377534] [MGI Ref ID J:121431]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry This strain is maintained as a homozygote.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2250.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Embryos
Price (US dollars $) Frozen Embryo $1600.00 Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryopreserved Embryos
Available to most shipping destinations1
This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.
1 Shipments cannot be made to Australia due to Australian government import restrictions.
2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2925.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Embryos
Price (US dollars $) Frozen Embryo $2080.00 Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryopreserved Embryos
Available to most shipping destinations1
This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.
1 Shipments cannot be made to Australia due to Australian government import restrictions.
2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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JAX® Mice, Products & Services Conditions of Use
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