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| When these mice are used in conjunction with a Cre recombinase-expressing strain, successful Cre-mediated excision results in the constitutive expression of mouse smoothened homolog (Drosophila) and unrestrained Hedgehog signaling in Cre-expressing tissues. As examples, this strain may be useful in studies of tumorigenic potential in the hedgehog pathway, progenitor cell involvement in the development of medulloblastomas, craniofacial development, thalamic development, and of oligodendrocyte maturation and CNS myelination when used in combination with specific cre-expressing strains | |||||||||||||||||||
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Description
Strain Information
Former Names STOCK Gt(ROSA)26Sortm1(Smo/YFP)Amc/J (Changed: 23-MAY-06 ) Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Homozygote (Female x Male) 01-MAR-06 Species laboratory mouse Generation N?+1F15 (27-NOV-11)
Generation DefinitionsDonating Investigator Andrew P McMahon, University of Southern California Description
These mice contain an Enhanced Yellow Fluorescent Protein/Smoothened homolog (Drosophila) fusion gene inserted into the Gt(ROSA)26Sor locus. The mutant allele consists of a fusion product involving Enhanced Yellow Fluorescent Protein (EYFP) and the constitutively active W539L point mutation of the mouse smoothened homolog (Drosophila) gene (SmoM2). Expression of the Smo/EYFP fusion gene is blocked by a loxP-flanked STOP fragment placed between the Gt(ROSA)26Sor promoter and the Smo/EYFP sequence. When used in conjunction with a Cre recombinase-expressing strain, successful Cre-mediated excision results in the constitutive expression of mouse smoothened homolog (Drosophila) and unrestrained Hedgehog signaling in Cre-expressing tissues. Expression of the SmoM2 fusion protein can be monitored using EYFP-specific fluorescence protocols. Mice that are homozygous for the mutant allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.For example, when crossed to a strain expressing tamoxifen inducible Cre recombinase in developing embryos (see Stock No. 004453), in neural progenitor cells (see Stock No. 007684), in cells that received positive hedgehog signalling (see Stock No. 007913), or in cells that express Shh (see Stock No. 005623), this mutant mouse strain may be useful in studies of tumorigenic potential in the hedgehog pathway.
When bred to a strain expressing Cre recombinase in the central nervous system(see Stock No. 004600 for example), this mutant mouse strain may be useful in studies of progenitor cell involvement in the development of medulloblastomas.
When bred to a strain expressing Cre recombinase in midbrain/dorsal spinal cord (see Stock No. 007807 or 009107 for example), this mutant mouse strain may be useful in studies of craniofacial development.
When bred to a strain expressing Cre recombinase in the nervous system (see Stock No. 003771 for example), this mutant mouse strain may be useful in studies of thalamic development.
When bred to a strain expressing Cre recombinase in oligodendrycytes (see Stock No. 011103 for example), this mutant mouse strain may be useful in studies of oligodendrocyte maturation and CNS myelination.
Development
A targeting vector containing a loxP flanked PGK-neo-stop cassette upstream of the Yellow Fluorescent Protein/Smoothened homolog (Drosophila) fusion gene (Smo/YFP) was inserted into the Gt(ROSA)26Sor locus. The construct was introduced into 129X1/SvJ-derived AV3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were bred to C57BL/6 and outbred Swiss Webster mice. (Additional information from Donating Investigator is forthcoming)
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
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Phenotype
Phenotype Information
- Model with phenotypic similarity to human disease where etiologies are distinct. Human genes are associated with this disease. Orthologs of these genes do not appear in the mouse genotype(s).
Medulloblastoma; MDB
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Gt(ROSA)26Sortm1(Smo/EYFP)Amc/?
involves: 129X1/SvJ
- tumorigenesis
- *normal* tumorigenesis
- no tumors are observed up to 12 months of age (MGI Ref ID J:114992)
The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.Gli1tm3(cre/ERT2)Alj/Gli1+ Gt(ROSA)26Sortm1(Smo/EYFP)Amc/Gt(ROSA)26Sortm1(Smo/EYFP)Amc
involves: 129S6/SvEvTac * 129X1/SvJ (conditional)
- mortality/aging
- premature death
- mice survive 45 days (MGI Ref ID J:139574)
- tumorigenesis
- medulloblastoma
- all mice develop medulloblastomas that are located in lobes VI through IX and have a mean survival of 45 days (MGI Ref ID J:139574)
Gt(ROSA)26Sortm1(Smo/EYFP)Amc/Gt(ROSA)26Sor+ Tg(Nes-cre)1Kln/0
involves: 129X1/SvJ * C57BL/6 * SJL (conditional)
- mortality/aging
- complete neonatal lethality
- animals die by the end of the first postnatal day (P0) (MGI Ref ID J:147427)
- nervous system phenotype
- abnormal thalamus morphology
- based on cell fate analysis and molecular marker analysis, the intergeniculate leaflet (IGL) nucleus is expanded caudodorsally along the surface of the diencephalon at E16.5 (MGI Ref ID J:147427)
- abnormal lateral geniculate nucleus morphology
- based on cell fate analysis and molecular marker analysis, the dorsal lateral geniculate (dLG) nucleus is expanded caudodorsally along the surface of the diencephalon at E16.5 (MGI Ref ID J:147427)
- increased brain size
- in all embryos, brain size is larger than in controls, especially in the dorsal telencephalon (MGI Ref ID J:147427)
Gt(ROSA)26Sortm1(Smo/EYFP)Amc/Gt(ROSA)26Sor+ Tg(Wnt1-cre)11Rth/0
involves: 129X1/SvJ * C57BL/6J * CBA/J (conditional)
- mortality/aging
- partial embryonic lethality during organogenesis
- half of mice die at E11.5 (MGI Ref ID J:135134)
- embryogenesis phenotype
- abnormal neural crest cell morphology
- the number of cardiac neural crest cells is increased in the cardiac jelly compared to in wild-type mice (MGI Ref ID J:135134)
- cardiovascular system phenotype
- abnormal outflow tract development
- at E11.5, surviving mice exhibit a lack of cushions in the distal outflow tract while more proximal outflow tract cushions are closer to each other unlike in wild-type mice (MGI Ref ID J:135134)
- persistent truncus arteriosis
- at E15.5 (MGI Ref ID J:135134)
- nervous system phenotype
- abnormal neural crest cell morphology
- the number of cardiac neural crest cells is increased in the cardiac jelly compared to in wild-type mice (MGI Ref ID J:135134)
Gt(ROSA)26Sortm1(Smo/EYFP)Amc/Gt(ROSA)26Sortm1(Smo/EYFP)Amc Olig2tm2(TVA,cre)Rth/Olig2+
involves: 129 * 129X1/SvJ
- mortality/aging
- premature death
- mice survive 33 days (MGI Ref ID J:139574)
- tumorigenesis
- medulloblastoma
- mice develop focal medulloblastomas localized to the posterior-lateral lobes and have mean survival of 33 days (MGI Ref ID J:139574)
Gt(ROSA)26Sortm1(Smo/EYFP)Amc/Gt(ROSA)26Sortm1(Smo/EYFP)Amc Shhtm2(cre/ERT2)Cjt/Shh+
involves: 129S6/SvEvTac * 129X1/SvJ (conditional)
- tumorigenesis
- *normal* tumorigenesis
- following induction with tamoxifen, mice do not exhibit medulloblastoma (MGI Ref ID J:139574)
Gt(ROSA)26Sortm1(Smo/EYFP)Amc/Gt(ROSA)26Sortm1(Smo/EYFP)Amc Tg(Atoh1-cre/Esr1*)14Fsh/0
involves: 129X1/SvJ * FVB/N (conditional)
- mortality/aging
- premature death
- mice survive 41 days (MGI Ref ID J:139574)
- nervous system phenotype
- abnormal cerebellum external granule cell layer morphology
- mice exhibit hyperplasia on the external granule cell layer beginning at P0 and more prominently at P7 (MGI Ref ID J:139574)
- tumorigenesis
- medulloblastoma
- all mice develop diffuse medulloblastomas and have a mean survival of 41 days (MGI Ref ID J:139574)
Gt(ROSA)26Sortm1(Smo/EYFP)Amc/Gt(ROSA)26Sortm1(Smo/EYFP)Amc Tg(GFAP-cre)25Mes/0
involves: 129X1/SvJ * FVB/N (conditional)
- mortality/aging
- premature death
- mice survive 57 days (MGI Ref ID J:139574)
- tumorigenesis
- medulloblastoma
- mice develop diffuse medulloblastoma tumors and have a mean survival of 57 days (MGI Ref ID J:139574)
Gt(ROSA)26Sortm1(Smo/EYFP)Amc/? Tg(CAG-cre/Esr1*)5Amc/?
involves: 129X1/SvJ * C57BL/6 * CBA (conditional)
- mortality/aging
- premature death
- following tamoxifen treatment, all mice are dead by 18 weeks of observation unlike untreated mice and Ptch1tm1Mps heterozygotes (MGI Ref ID J:114992)
- tumorigenesis
- basal cell carcinoma
- following tamoxifen treatment, mice exhibit macroscopic basal cell carcinomas (BBC) where as all other mice develop BBC tumors at 8 weeks (MGI Ref ID J:114992)
- medulloblastoma
- found in 27% of mice and 40% of mice following tamoxifen treatment (MGI Ref ID J:114992)
- rhabdomyosarcoma
- present without tamoxifen treatment (average number 3) (MGI Ref ID J:114992)
- following tamoxifen treatment, the multiplicity of tumors is increased (average number 7) (MGI Ref ID J:114992)
- mostly confined to rear thigh and abdominal wall (MGI Ref ID J:114992)
- following tamoxifen treatment, tumors are detected in skeletal muscle of the head, neck, tongue and paratesticular regions (MGI Ref ID J:114992)
- following tamoxifen treatment at P10, age of onset is accelerated to week 5 compared to week 9 in untreated mice (MGI Ref ID J:114992)
- digestive/alimentary phenotype
- gastric polyps
- diverticular harmatomatous lesions in the stomach occur at a higher rate following treatment with tamoxifen (less than 5% of mice after treatment) (MGI Ref ID J:114992)
- intestine polyps
- diverticular harmatomatous lesions in the intestine occur at a higher rate following treatment with tamoxifen (20% without treatment and 80% following treatment) (MGI Ref ID J:114992)
- endocrine/exocrine gland phenotype
- abnormal pancreas morphology
- cystic metaplastic lesions are observed with increased frequency following tamoxifen treatment (MGI Ref ID J:114992)
- integument phenotype
- basal cell carcinoma
- following tamoxifen treatment, mice exhibit macroscopic basal cell carcinomas (BBC) where as all other mice develop BBC tumors at 8 weeks (MGI Ref ID J:114992)
Gt(ROSA)26Sortm1(Smo/EYFP)Amc/? Tg(Wnt1-cre)11Rth/?
involves: 129X1/SvJ * C57BL/6J * CBA/J (conditional)This mouse can be used to support research in many areas including:
Gt(ROSA)26Sortm1(Smo/EYFP)Amc relatedNeurobiology Research
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Fluorescent Proteins
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Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Gt(ROSA)26Sortm1(Smo/EYFP)Amc | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Andrew P McMahon | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | Gt(ROSA)26Sortm1(smo/YFP)Amc; R26-lsl-SmoM2; R26SmoM2; R26SmoM2; SmoOEX; SmoM2; SmoM2-YFPfl; SmoM2/Yfp; SmoM2fl; | ||
| Mutation Made By | Junhao Mao, Harvard University | ||
| Strain of Origin | 129X1/SvJ | ||
| ES Cell Line Name | AV3 | ||
| ES Cell Line Strain | 129X1/SvJ | ||
| Site of Expression | when crossed to a Cre recombinase-expressing strain, expression of Enhanced Yellow Fluorescent Protein and the mouse smoothened homolog (Drosophila) protein is observed in the cre-expressing tissues, leading to unrestrained Hedgehog signaling | ||
| Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish | ||
| Yellow Fluorescent Protein (YFP) is a derivative of Green Fluorescent Protein (GFP), a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance fluorescence intensity and shift the wavelength emitted when excited. When YFP is utilized in a transgenic construct, tissue expressing sufficient amounts of YFP will fluoresce yellowish-green when exposed to a 513 nm light source. | |||
| General Note | Phenotypic Similarity to Human Syndrome: Medullablastoma (J:139574) | ||
| Molecular Note | A loxP flanked PGK-neo-stop cassette upstream of the Yellow Fluorescent Protein/Smoothened homolog (Drosophila) fusion gene (Smo/EYFP) was inserted into the Gt(ROSA)26Sor locus. The mutant allele consists of a fusion product involving Yellow FluorescentProtein and the constitutively active W539L point mutation of the mouse smoothened homolog (Drosophila) gene (SmoM2). Expression of the Smo/EYFP fusion gene is blocked by a loxP-flanked STOP fragment placed between the Gt(ROSA)26Sor promoter and the Smo/EYFP sequence. [MGI Ref ID J:89445] | ||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gt(ROSA)26Sortm1(Smo/EYFP)Amc, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Jeong J; Mao J; Tenzen T; Kottmann AH; McMahon AP. 2004. Hedgehog signaling in the neural crest cells regulates the patterning and growth of facial primordia. Genes Dev 18(8):937-51. [PubMed: 15107405] [MGI Ref ID J:89445]
Additional References
Gt(ROSA)26Sortm1(Smo/EYFP)Amc relatedBluske KK; Kawakami Y; Koyano-Nakagawa N; Nakagawa Y. 2009. Differential activity of Wnt/beta-catenin signaling in the embryonic mouse thalamus. Dev Dyn 238(12):3297-3309. [PubMed: 19924825] [MGI Ref ID J:154363]
Flora A; Klisch TJ; Schuster G; Zoghbi HY. 2009. Deletion of Atoh1 disrupts Sonic Hedgehog signaling in the developing cerebellum and prevents medulloblastoma. Science 326(5958):1424-7. [PubMed: 19965762] [MGI Ref ID J:155047]
Franco HL; Lee KY; Rubel CA; Creighton CJ; White LD; Broaddus RR; Lewis MT; Lydon JP; Jeong JW; DeMayo FJ. 2010. Constitutive activation of smoothened leads to female infertility and altered uterine differentiation in the mouse. Biol Reprod 82(5):991-9. [PubMed: 20130264] [MGI Ref ID J:159714]
Frick A; Grammel D; Schmidt F; Poschl J; Priller M; Pagella P; von Bueren AO; Peraud A; Tonn JC; Herms J; Rutkowski S; Kretzschmar HA; Schuller U. 2012. Proper cerebellar development requires expression of beta1-integrin in Bergmann glia, but not in granule neurons. Glia 60(5):820-32. [PubMed: 22374686] [MGI Ref ID J:181620]
Gao J; Graves S; Koch U; Liu S; Jankovic V; Buonamici S; El Andaloussi A; Nimer SD; Kee BL; Taichman R; Radtke F; Aifantis I. 2009. Hedgehog signaling is dispensable for adult hematopoietic stem cell function. Cell Stem Cell 4(6):548-58. [PubMed: 19497283] [MGI Ref ID J:149820]
Goddeeris MM; Schwartz R; Klingensmith J; Meyers EN. 2007. Independent requirements for Hedgehog signaling by both the anterior heart field and neural crest cells for outflow tract development. Development 134(8):1593-604. [PubMed: 17344228] [MGI Ref ID J:135134]
Gu D; Fan Q; Zhang X; Xie J. 2012. A role for transcription factor STAT3 signaling in oncogene smoothened-driven carcinogenesis. J Biol Chem 287(45):38356-66. [PubMed: 22992748] [MGI Ref ID J:192468]
Han YG; Kim HJ; Dlugosz AA; Ellison DW; Gilbertson RJ; Alvarez-Buylla A. 2009. Dual and opposing roles of primary cilia in medulloblastoma development. Nat Med 15(9):1062-5. [PubMed: 19701203] [MGI Ref ID J:154130]
Han YG; Spassky N; Romaguera-Ros M; Garcia-Verdugo JM; Aguilar A; Schneider-Maunoury S; Alvarez-Buylla A. 2008. Hedgehog signaling and primary cilia are required for the formation of adult neural stem cells. Nat Neurosci 11(3):277-84. [PubMed: 18297065] [MGI Ref ID J:135664]
Haraguchi R; Matsumaru D; Nakagata N; Miyagawa S; Suzuki K; Kitazawa S; Yamada G. 2012. The hedgehog signal induced modulation of bone morphogenetic protein signaling: an essential signaling relay for urinary tract morphogenesis. PLoS One 7(7):e42245. [PubMed: 22860096] [MGI Ref ID J:189675]
Hatley ME; Tang W; Garcia MR; Finkelstein D; Millay DP; Liu N; Graff J; Galindo RL; Olson EN. 2012. A mouse model of rhabdomyosarcoma originating from the adipocyte lineage. Cancer Cell 22(4):536-46. [PubMed: 23079662] [MGI Ref ID J:192027]
Heine VM; Priller M; Ling J; Rowitch DH; Schuller U. 2010. Dexamethasone destabilizes Nmyc to inhibit the growth of hedgehog-associated medulloblastoma. Cancer Res 70(13):5220-5. [PubMed: 20530674] [MGI Ref ID J:161601]
Heine VM; Rowitch DH. 2009. Hedgehog signaling has a protective effect in glucocorticoid-induced mouse neonatal brain injury through an 11betaHSD2-dependent mechanism. J Clin Invest 119(2):267-77. [PubMed: 19164857] [MGI Ref ID J:146149]
Huang X; Dubuc AM; Hashizume R; Berg J; He Y; Wang J; Chiang C; Cooper MK; Northcott PA; Taylor MD; Barnes MJ; Tihan T; Chen J; Hackett CS; Weiss WA; James CD; Rowitch DH; Shuman MA; Jan YN; Jan LY. 2012. Voltage-gated potassium channel EAG2 controls mitotic entry and tumor growth in medulloblastoma via regulating cell volume dynamics. Genes Dev 26(16):1780-96. [PubMed: 22855790] [MGI Ref ID J:186629]
Huang X; Ketova T; Fleming JT; Wang H; Dey SK; Litingtung Y; Chiang C. 2009. Sonic hedgehog signaling regulates a novel epithelial progenitor domain of the hindbrain choroid plexus. Development 136(15):2535-43. [PubMed: 19570847] [MGI Ref ID J:152851]
Huang X; Liu J; Ketova T; Fleming JT; Grover VK; Cooper MK; Litingtung Y; Chiang C. 2010. Transventricular delivery of Sonic hedgehog is essential to cerebellar ventricular zone development. Proc Natl Acad Sci U S A 107(18):8422-7. [PubMed: 20400693] [MGI Ref ID J:160335]
Hwang SH; Lee H; Yamamoto M; Jones LA; Dayalan J; Hopkins R; Zhou XJ; Yarovinsky F; Connolly JE; Curotto de Lafaille MA; Wakeland EK; Fairhurst AM. 2012. B Cell TLR7 Expression Drives Anti-RNA Autoantibody Production and Exacerbates Disease in Systemic Lupus Erythematosus-Prone Mice. J Immunol 189(12):5786-96. [PubMed: 23150717] [MGI Ref ID J:190857]
Hyman JM; Firestone AJ; Heine VM; Zhao Y; Ocasio CA; Han K; Sun M; Rack PG; Sinha S; Wu JJ; Solow-Cordero DE; Jiang J; Rowitch DH; Chen JK. 2009. Small-molecule inhibitors reveal multiple strategies for Hedgehog pathway blockade. Proc Natl Acad Sci U S A 106(33):14132-7. [PubMed: 19666565] [MGI Ref ID J:151950]
Kerr CL; Huang J; Williams T; West-Mays JA. 2012. Activation of the hedgehog signaling pathway in the developing lens stimulates ectopic FoxE3 expression and disruption in fiber cell differentiation. Invest Ophthalmol Vis Sci 53(7):3316-30. [PubMed: 22491411] [MGI Ref ID J:196835]
Li Y; Gordon J; Manley NR; Litingtung Y; Chiang C. 2008. Bmp4 is required for tracheal formation: a novel mouse model for tracheal agenesis. Dev Biol 322(1):145-55. [PubMed: 18692041] [MGI Ref ID J:142133]
Lin AC; Seeto BL; Bartoszko JM; Khoury MA; Whetstone H; Ho L; Hsu C; Ali AS; Alman BA. 2009. Modulating hedgehog signaling can attenuate the severity of osteoarthritis. Nat Med 15(12):1421-5. [PubMed: 19915594] [MGI Ref ID J:155889]
Lin C; Yin Y; Veith GM; Fisher AV; Long F; Ma L. 2009. Temporal and spatial dissection of Shh signaling in genital tubercle development. Development 136(23):3959-67. [PubMed: 19906863] [MGI Ref ID J:158288]
Mao J; Kim BM; Rajurkar M; Shivdasani RA; McMahon AP. 2010. Hedgehog signaling controls mesenchymal growth in the developing mammalian digestive tract. Development 137(10):1721-9. [PubMed: 20430747] [MGI Ref ID J:160363]
Mao J; Ligon KL; Rakhlin EY; Thayer SP; Bronson RT; Rowitch D; McMahon AP. 2006. A novel somatic mouse model to survey tumorigenic potential applied to the Hedgehog pathway. Cancer Res 66(20):10171-8. [PubMed: 17047082] [MGI Ref ID J:114992]
Matsumaru D; Haraguchi R; Miyagawa S; Motoyama J; Nakagata N; Meijlink F; Yamada G. 2011. Genetic analysis of Hedgehog signaling in ventral body wall development and the onset of omphalocele formation. PLoS One 6(1):e16260. [PubMed: 21283718] [MGI Ref ID J:169568]
Migone FF; Ren Y; Cowan RG; Harman RM; Nikitin AY; Quirk SM. 2012. Dominant activation of the hedgehog signaling pathway alters development of the female reproductive tract. Genesis 50(1):28-40. [PubMed: 21809434] [MGI Ref ID J:181061]
Miyagawa S; Matsumaru D; Murashima A; Omori A; Satoh Y; Haraguchi R; Motoyama J; Iguchi T; Nakagata N; Hui CC; Yamada G. 2011. The role of sonic hedgehog-gli2 pathway in the masculinization of external genitalia. Endocrinology 152(7):2894-903. [PubMed: 21586556] [MGI Ref ID J:174885]
Nielsen CM; Dymecki SM. 2010. Sonic hedgehog is required for vascular outgrowth in the hindbrain choroid plexus. Dev Biol 340(2):430-7. [PubMed: 20123094] [MGI Ref ID J:160263]
Nitzki F; Zibat A; Konig S; Wijgerde M; Rosenberger A; Brembeck FH; Carstens PO; Frommhold A; Uhmann A; Klingler S; Reifenberger J; Pukrop T; Aberger F; Schulz-Schaeffer W; Hahn H. 2010. Tumor stroma-derived Wnt5a induces differentiation of basal cell carcinoma of Ptch-mutant mice via CaMKII. Cancer Res 70(7):2739-48. [PubMed: 20233865] [MGI Ref ID J:158915]
Olsen O; Funke L; Long JF; Fukata M; Kazuta T; Trinidad JC; Moore KA; Misawa H; Welling PA; Burlingame AL; Zhang M; Bredt DS. 2007. Renal defects associated with improper polarization of the CRB and DLG polarity complexes in MALS-3 knockout mice. J Cell Biol 179(1):151-64. [PubMed: 17923534] [MGI Ref ID J:134806]
Park KS; Martelotto LG; Peifer M; Sos ML; Karnezis AN; Mahjoub MR; Bernard K; Conklin JF; Szczepny A; Yuan J; Guo R; Ospina B; Falzon J; Bennett S; Brown TJ; Markovic A; Devereux WL; Ocasio CA; Chen JK; Stearns T; Thomas RK; Dorsch M; Buonamici S; Watkins DN; Peacock CD; Sage J. 2011. A crucial requirement for Hedgehog signaling in small cell lung cancer. Nat Med 17(11):1504-8. [PubMed: 21983857] [MGI Ref ID J:178123]
Poschl J; Lorenz A; Hartmann W; von Bueren AO; Kool M; Li S; Peraud A; Tonn JC; Herms J; Xiang M; Rutkowski S; Kretzschmar HA; Schuller U. 2011. Expression of BARHL1 in medulloblastoma is associated with prolonged survival in mice and humans. Oncogene 30(47):4721-30. [PubMed: 21602885] [MGI Ref ID J:178575]
Ren Y; Cowan RG; Harman RM; Quirk SM. 2009. Dominant activation of the hedgehog signaling pathway in the ovary alters theca development and prevents ovulation. Mol Endocrinol 23(5):711-23. [PubMed: 19196835] [MGI Ref ID J:147787]
Ren Y; Cowan RG; Migone FF; Quirk SM. 2012. Overactivation of hedgehog signaling alters development of the ovarian vasculature in mice. Biol Reprod 86(6):174. [PubMed: 22402963] [MGI Ref ID J:185828]
Schuller U; Heine VM; Mao J; Kho AT; Dillon AK; Han YG; Huillard E; Sun T; Ligon AH; Qian Y; Ma Q; Alvarez-Buylla A; McMahon AP; Rowitch DH; Ligon KL. 2008. Acquisition of granule neuron precursor identity is a critical determinant of progenitor cell competence to form Shh-induced medulloblastoma. Cancer Cell 14(2):123-34. [PubMed: 18691547] [MGI Ref ID J:139574]
Spassov DS; Wong CH; Wong SY; Reiter JF; Moasser MM. 2013. Trask loss enhances tumorigenic growth by liberating integrin signaling and growth factor receptor cross-talk in unanchored cells. Cancer Res 73(3):1168-79. [PubMed: 23243018] [MGI Ref ID J:194368]
Visbal AP; LaMarca HL; Villanueva H; Toneff MJ; Li Y; Rosen JM; Lewis MT. 2011. Altered differentiation and paracrine stimulation of mammary epithelial cell proliferation by conditionally activated Smoothened. Dev Biol 352(1):116-27. [PubMed: 21276786] [MGI Ref ID J:171474]
Vue TY; Bluske K; Alishahi A; Yang LL; Koyano-Nakagawa N; Novitch B; Nakagawa Y. 2009. Sonic hedgehog signaling controls thalamic progenitor identity and nuclei specification in mice. J Neurosci 29(14):4484-97. [PubMed: 19357274] [MGI Ref ID J:147427]
Wong SY; Reiter JF. 2011. From the Cover: Wounding mobilizes hair follicle stem cells to form tumors. Proc Natl Acad Sci U S A 108(10):4093-8. [PubMed: 21321207] [MGI Ref ID J:170482]
Wong SY; Seol AD; So PL; Ermilov AN; Bichakjian CK; Epstein EH Jr; Dlugosz AA; Reiter JF. 2009. Primary cilia can both mediate and suppress Hedgehog pathway-dependent tumorigenesis. Nat Med 15(9):1055-61. [PubMed: 19701205] [MGI Ref ID J:154128]
Youssef KK; Lapouge G; Bouvree K; Rorive S; Brohee S; Appelstein O; Larsimont JC; Sukumaran V; Van de Sande B; Pucci D; Dekoninck S; Berthe JV; Aerts S; Salmon I; del Marmol V; Blanpain C. 2012. Adult interfollicular tumour-initiating cells are reprogrammed into an embryonic hair follicle progenitor-like fate during basal cell carcinoma initiation. Nat Cell Biol 14(12):1282-94. [PubMed: 23178882] [MGI Ref ID J:195242]
Youssef KK; Van Keymeulen A; Lapouge G; Beck B; Michaux C; Achouri Y; Sotiropoulou PA; Blanpain C. 2010. Identification of the cell lineage at the origin of basal cell carcinoma. Nat Cell Biol 12(3):299-305. [PubMed: 20154679] [MGI Ref ID J:195334]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Mating System Homozygote x Homozygote (Female x Male) 01-MAR-06 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $195.00 Female or Male Homozygous for Gt(ROSA)26Sortm1(Smo/EYFP)Amc
Price per Pair (US dollars $) Pair Genotype $390.00 Homozygous for Gt(ROSA)26Sortm1(Smo/EYFP)Amc x Homozygous for Gt(ROSA)26Sortm1(Smo/EYFP)Amc Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
| Pricing for International shipping destinations |
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Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $253.50 Female or Male Homozygous for Gt(ROSA)26Sortm1(Smo/EYFP)Amc
Price per Pair (US dollars $) Pair Genotype $507.00 Homozygous for Gt(ROSA)26Sortm1(Smo/EYFP)Amc x Homozygous for Gt(ROSA)26Sortm1(Smo/EYFP)Amc Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
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Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
JAX® Mice
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JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
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Terms of Use
Terms of Use
General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.