Strain Name: |
C3.B6-Tg(Fabp1-Ccnd1)4Rdb/J |
|---|---|
Stock Number: |
005143 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | Ccnd1; Fabp1; Tg(Fabp1-Ccnd1)4Rdb; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator IMR Colony, The Jackson Laboratory Generation N7pN1 Strain Description
These transgenic mice express mouse cyclin D1 under the direction of the rat fatty acid binding protein 1 promoter (-596 to +21 fragment). Mice that are hemizygous for the transgene are viable, normal in size and do not display any gross physical or behavioral abnormalities. Hemizygous mutant mice exhibit reduced fertility after 3 months of age. Transgenic mice overexpress cyclin D1 in the liver and epithelial cells of the small intestine and colon. A lower level of expression occurs in the kidneys. Onset of progressive liver disease begins at one month of age with hepatomegaly. Male transgenic mice exhibit more severe hepatomegaly than transgenic females. By 6 months of age, all transgenic males and half of the transgenic females display hepatocellular dysplasia. Eighty-seven percent of transgenic males and 69% of transgenic females develop hepatocellular adenoma or hepatocellular carcinoma by 15 months of age. This mutant mouse strain may be useful in studies of hepatocellular carcinogenesis.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Strain Development
A transgenic construct containing a mouse cyclin D1 gene inserted into sequence encoding the rabbit Beta- globin under the control of the rat fatty acid binding protein 1 promoter was injected into fertilized C57BL/6 mouse eggs. Founder animals were backcrossed at least twice to C57BL/6 and then backcrossed to C3H for 5 generations.
Gene & Allele Details
| Allele Symbol | Tg(Fabp1-Ccnd1)4Rdb | ||
|---|---|---|---|
| Allele Name | transgene insertion 4, R Daniel Beauchamp | ||
| Mutation Made By | Natasha Deane, Vanderbilt University Medical Center | ||
| Strain of Origin | C57BL/6 | ||
| Expressed Gene | Ccnd1, cyclin D1, mouse, laboratory | ||
| Promoter | Fabp1, fatty acid binding protein 1, liver, rat | ||
| General Note | Hemizygous transgenic mice are viable, normal in size, and do not display any gross physical or behavioral abnormalities. These mice exhibit reduced fertility after 3 months of age. Onset of progressive liver disease begins at one month of age with hepatomegaly. Male transgenic mice exhibit more severe hepatomegaly than transgenic females. By 6 months of age, all transgenic males and half of the transgenic females display hepatocellular dysplasia. 87% of transgenic males and 69% of transgenic females develop hepatocellular adenoma or hepatocellular carcinoma by 15 months of age. This mutant mouse strain may be useful in studies of hepatocellular carcinogenesis. | ||
| Molecular Note | This transgenic construct contains the mouse cyclin D1 gene driven by the rat fatty acid binding protein promoter. Transgenic mice overexpress cyclin D1 in the liver and epithelial cells of the small intestine and colon. A lower level of expression occurs in the kidneys. [MGI Ref ID J:70394] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Tg(Fabp1-Ccnd1)4Rdb
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice are bred as hemizygotes. |
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Related Strains
Strains carrying Tg(Fabp1-Ccnd1)4Rdb allele
004834 C57BL/6-Tg(Fabp1-Ccnd1)4Rdb/J View Strains carrying Tg(Fabp1-Ccnd1)4Rdb (1 strain)
002537 B6;129S2-Ccnd1tm1Wbg/J 002935 FVB.129S2(B6)-Ccnd1tm1Wbg/J
Additional Web Information
Congenic Nomenclature
Genetic Quality Control Annual Report
Research Applications
This mouse can be used to support research in many areas including:
Cancer Research
Increased Tumor Incidence (Adenomas)
Increased Tumor Incidence (Hepatomas: hepatacellular carcinoma)
Reproductive Biology Research
Fertility Defects
References
Selected Reference(s)
Additional ReferencesDeane NG; Parker MA; Aramandla R; Diehl L; Lee WJ; Washington MK; Nanney LB; Shyr Y; Beauchamp RD. 2001. Hepatocellular carcinoma results from chronic cyclin D1 overexpression in transgenic mice. Cancer Res 61(14):5389-95. [PubMed: 11454681] [MGI Ref ID J:70394]
Price and Supply Information
| Strain Name: | C3.B6-Tg(Fabp1-Ccnd1)4Rdb/J |
| Stock Number: | 005143 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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